Rw5G040860

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016128
Physical Location & Seq
Forward (+)
72111320 .. 72113846
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040860.1

Sequence Viewer

Length: 1554 bp
ATGGCTGCAACTCTCACTTCCAATTCATTCGTTCTAACTTCCTCACCCAATTCAAGAGCAACAACCACTCTCAGGAACCCCCGAAGGTTCACCGTCTTTGCCAAGAAGGCTGGACCCTTCTCTACATTTCGGCTTGGCAAGTCATCAGGGGATGTTGGTTCATCGTCCTCCGGCGAAGGCGAAGCCGATGACTCCAATTCAAGTCCATTCAGGTTTAATTTCGGGAAATTACCCGATGTCAAGTCTTTGATCCCCGTTGCGAGTAGGCCTTCTTTTTCATTTGGGACCTCCAGAAGCAAGGACCCTGGGACTGTGTTTGTTGCTGGTGCAACAGGGCAGGCTGGGGTTCGAATAGCACAGACCCTTCTGCGTGAAGGTTTTAGAGTGAGGGCTGGTGTGCCTGAACTGGGTGATGCCCAAGAATTGGCTCTTGTTGCTTCTAAATACAAGATCCTATCAAATGAGGAATCAAAACGCCTCAATGCTGTGGGGTCTACCTTCGAAGATGCTGAGACTATCGCCAAAGCCATCGGGAATGCCAGCAAAGTTGTTGTCACAATTGGCCCCTTCGAGAATGGTCCAACGACTGAAGTCACACCATCAGACGCCTTACAAGTAATCCAAGCTGCTCAGCTCGCCGGTGTCGGTCATGTGGCCATCGTCTATGATGGTAACTTAGCAGGTGCATCGACTTACAATGTAGTAGATGGCATATCTTCATTCTTTAGCAACATCTTCTCGCGATCTCAGCCGTTGAGCATCACTGAATTGTTGCAAAAAGTCATTCAAACAGACGTTAGCTACACTTTCATAAAGACAAGTTTGACGGATGATTTTTCGCCCGAGAGCTCGTATAATGTTGTTGTGTCAGCTGAAGGAAACGGAGGTGCTAACGACTACAAAGTAGCCAAGTCTCAGATTGCTTCCCTGGTGGCAGATGTTTTCTCAAACACTTCTGTGGCAGAAAATAAGGTTGTGGAGGTATATACTGATCCATCAGCACCCTTGAGGCCTGTAGATCAACTTTTCAGTACAATCCCTGAGGATGGAAGAAGGAAAGCTTATGCAGAAGCGATTGCAAAGGCAAAGGCAGAAGAAGAGGCAATAATAGCTGCTGAGAGATCTCGCAAAGCAGCCGAGGCAGCAAGGTTACTTGAAGAAGAAGTAAAGAAGCTATCCGAACAAGAAGCTCAAGCCTCAAGTTTGGCAGAAGAAGCTCAAGTAAAGGCTGAGGCTGCAGGGGCCTCATTTGATGGCCTCGTAAGCAAAGCAAAAGACTTTGGCTCAGGGCTTTCTTGGGATAAGTTTAGCTCACAACTTGCAGACACAGTTTCAAAATCCATGGAGACACCAAAAGTTCAGATTGCTACTGTAAGGGGACAGGCCAAGGCTAAGACACTGCCTCCGCTGAAAGCAGTTGCTAAGAAAGTTGCAACTCCGAAACCAAAGCAGGAGGCAAAGCCTAAATCCAAACAAACAGAGGCGAAGACTGAAGTGAGGAAGGTATTTGGTGGCTTGTTCACACAAGAGACTGTGTATGTTGATGATGAATGA

Protein Analysis

517

Amino Acids

54.77

Weight (kDa)

7.67

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 104 - 218 7.6e-06 NmrA-like family
NAD_binding_10 PF13460 109 - 317 1.6e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46780
fragaria_vesca FvH4_3g36950
malus_domestica MD03G1086000.v1.1
prunus_persica Prupe.6G070200_v2.0.a1
pyrus_communis pycom03g06790 pycom11g08030
rosa_chinensis RchiOBHm_Chr5g0066371
rosa_laevigata RLG00000035855
rosa_multiflora Rmu_ssc0000249.1_g000009
rosa_roxburghii Rroxscaffold_1G00014770
rosa_samantha Rh5AG436200 Rh5BG452600 Rh5CG475000 Rh5DG467800
rosa_wichuraiana Rw5G040860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 424
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 742
AciI CCGC 1 cut(s) 1406
AclWI GGATC 3 cut(s) 244, 445, 986
AcoI YGGCCR 1 cut(s) 654
AcuI CTGAAG 3 cut(s) 609, 894, 1512
AcyI GRCGYC 1 cut(s) 606
AfaI GTAC 1 cut(s) 1033
AfiI CCNNNNNNNGG 4 cut(s) 72, 407, 424, 1046
AgsI TTSAA 5 cut(s) 54, 201, 788, 1157, 1335
AjnI CCWGG 2 cut(s) 304, 927
AleI CACNNNNGTG 1 cut(s) 956
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 4 cut(s) 506, 918, 1340, 1523
AlwI GGATC 3 cut(s) 244, 445, 986
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 7 cut(s) 266, 562, 654, 1010, 1242, 1255, 1383
ApeKI GCWGC 6 cut(s) 5, 626, 1112, 1133, 1142, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AspS9I GGNCC 6 cut(s) 113, 285, 301, 563, 578, 1242
AsuHPI GGTGA 3 cut(s) 36, 82, 422
AsuII TTCGAA 2 cut(s) 349, 501
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 4 cut(s) 113, 285, 301, 578
AxyI CCTNAGG 1 cut(s) 1041
BalI TGGCCA 1 cut(s) 656
BanII GRGCYC 1 cut(s) 851
BbsI GAAGAC 1 cut(s) 1493
Bbv12I GWGCWC 1 cut(s) 851
BbvCI CCTCAGC 1 cut(s) 1230
BbvI GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1222
BccI CCATC 8 cut(s) 536, 607, 662, 665, 701, 1003, 1040, 1247
BceAI ACGGC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 2 cut(s) 306, 929
BcoDI GTCTC 4 cut(s) 506, 918, 1340, 1523
BfmI CTRYAG 2 cut(s) 1014, 1236
BfuAI ACCTGC 1 cut(s) 671
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 1 cut(s) 1121
BisI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1236
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 6 cut(s) 7, 628, 1114, 1135, 1144, 1237
Bme1390I CCNGG 2 cut(s) 306, 929
Bme18I GGWCC 4 cut(s) 113, 285, 301, 578
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 6 cut(s) 113, 285, 301, 563, 578, 1242
BmiI GGNNCC 6 cut(s) 77, 115, 286, 303, 565, 1243
BmrFI CCNGG 2 cut(s) 306, 929
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 4 cut(s) 403, 496, 695, 768
BmuI ACTGGG 1 cut(s) 416
BoxI GACNNNNGTC 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 1493
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 2 cut(s) 1230, 1285
Bpu1102I GCTNAGC 1 cut(s) 630
Bpu14I TTCGAA 2 cut(s) 349, 501
BpuEI CTTGAG 4 cut(s) 1027, 1176, 1183, 1203
BsaHI GRCGYC 1 cut(s) 606
BsaJI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1341, 1386
BsaXI ACNNNNNCTCC 2 cut(s) 1387, 1417
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 407, 424, 1046
Bse118I RCCGGY 1 cut(s) 638
Bse1I ACTGG 1 cut(s) 411
Bse21I CCTNAGG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 306, 929
BseDI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1341, 1386
BseGI GGATG 3 cut(s) 157, 835, 1051
BseLI CCNNNNNNNGG 4 cut(s) 72, 407, 424, 1046
BseMII CTCAG 9 cut(s) 85, 501, 644, 761, 929, 1032, 1107, 1221, 1299
BseNI ACTGG 1 cut(s) 411
BseXI GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1222
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 7 cut(s) 268, 564, 656, 1012, 1244, 1257, 1385
BsiHKAI GWGCWC 1 cut(s) 851
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 2 cut(s) 171, 639
BslFI GGGAC 3 cut(s) 298, 322, 1392
BslI CCNNNNNNNGG 4 cut(s) 72, 407, 424, 1046
BsmAI GTCTC 4 cut(s) 506, 918, 1340, 1523
BsmFI GGGAC 3 cut(s) 298, 322, 1392
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 7 cut(s) 268, 564, 656, 1012, 1244, 1257, 1385
BsoBI CYCGRG 1 cut(s) 842
Bsp119I TTCGAA 2 cut(s) 349, 501
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 1341
Bsp68I TCGCGA 1 cut(s) 742
BspACI CCGC 1 cut(s) 1406
BspANI GGCC 7 cut(s) 268, 564, 656, 1012, 1244, 1257, 1385
BspCNI CTCAG 9 cut(s) 84, 502, 643, 760, 928, 1033, 1108, 1222, 1298
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 6 cut(s) 77, 115, 286, 303, 565, 1243
BspMAI CTGCAG 1 cut(s) 1240
BspMI ACCTGC 1 cut(s) 671
BspPI GGATC 3 cut(s) 244, 445, 986
BspT104I TTCGAA 2 cut(s) 349, 501
BsrFI RCCGGY 1 cut(s) 638
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 638
BssECI CCNNGG 6 cut(s) 304, 305, 927, 1137, 1341, 1386
BssMI GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
BssNI GRCGYC 1 cut(s) 606
BssT1I CCWWGG 2 cut(s) 1341, 1386
Bst2UI CCWGG 2 cut(s) 306, 929
Bst4CI ACNGT 5 cut(s) 94, 313, 1330, 1372, 1534
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 1 cut(s) 606
BstBI TTCGAA 2 cut(s) 349, 501
BstC8I GCNNGC 3 cut(s) 339, 541, 636
BstDSI CCRYGG 1 cut(s) 1341
BstF5I GGATG 3 cut(s) 157, 835, 1051
BstFNI CGCG 1 cut(s) 742
BstKTI GATC 6 cut(s) 252, 453, 746, 994, 1021, 1124
BstMAI GTCTC 4 cut(s) 506, 918, 1340, 1523
BstMBI GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
BstNI CCWGG 2 cut(s) 306, 929
BstPAI GACNNNNGTC 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 304, 927
BstSFI CTRYAG 2 cut(s) 1014, 1236
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1222
BstV2I GAAGAC 1 cut(s) 1493
BstX2I RGATCY 2 cut(s) 450, 1121
BstYI RGATCY 2 cut(s) 450, 1121
Bsu36I CCTNAGG 1 cut(s) 1041
BsuRI GGCC 7 cut(s) 268, 564, 656, 1012, 1244, 1257, 1385
BtgI CCRYGG 1 cut(s) 1341
BtsCI GGATG 3 cut(s) 157, 835, 1051
BtsI GCAGTG 1 cut(s) 1397
BtsIMutI CAGTG 2 cut(s) 762, 1397
BtuMI TCGCGA 1 cut(s) 742
BveI ACCTGC 1 cut(s) 671
Cac8I GCNNGC 3 cut(s) 339, 541, 636
Cfr10I RCCGGY 1 cut(s) 638
Cfr13I GGNCC 6 cut(s) 113, 285, 301, 563, 578, 1242
CseI GACGC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 1032
CviAII CATG 2 cut(s) 650, 1342
CviQI GTAC 1 cut(s) 1032
DpnI GATC 6 cut(s) 251, 452, 745, 993, 1020, 1123
DpnII GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
Ecl136II GAGCTC 1 cut(s) 849
Eco130I CCWWGG 2 cut(s) 1341, 1386
Eco147I AGGCCT 2 cut(s) 268, 1012
Eco24I GRGCYC 1 cut(s) 851
Eco47I GGWCC 4 cut(s) 113, 285, 301, 578
Eco53kI GAGCTC 1 cut(s) 849
Eco57I CTGAAG 3 cut(s) 609, 894, 1512
Eco81I CCTNAGG 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 842
EcoICRI GAGCTC 1 cut(s) 849
EcoO109I RGGNCCY 3 cut(s) 285, 301, 1242
EcoRII CCWGG 2 cut(s) 304, 927
EcoT14I CCWWGG 2 cut(s) 1341, 1386
EcoT38I GRGCYC 1 cut(s) 851
ErhI CCWWGG 2 cut(s) 1341, 1386
FaeI CATG 2 cut(s) 653, 1345
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 3 cut(s) 298, 322, 1392
FatI CATG 2 cut(s) 649, 1341
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1236
FokI GGATG 3 cut(s) 164, 842, 1058
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1236
GluI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1236
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 7 cut(s) 268, 564, 656, 1012, 1244, 1257, 1385
HapII CCGG 2 cut(s) 171, 639
HgaI GACGC 1 cut(s) 614
Hin1I GRCGYC 1 cut(s) 606
Hin1II CATG 2 cut(s) 653, 1345
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 2 cut(s) 191, 467
HpaII CCGG 2 cut(s) 171, 639
HphI GGTGA 3 cut(s) 36, 82, 422
Hpy166II GTNNAC 3 cut(s) 90, 495, 1521
Hpy188I TCNGA 5 cut(s) 604, 918, 1180, 1362, 1440
Hpy188III TCNNGA 7 cut(s) 54, 73, 223, 291, 532, 571, 741
Hpy8I GTNNAC 3 cut(s) 90, 495, 1521
HpyCH4III ACNGT 5 cut(s) 94, 313, 1330, 1372, 1534
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 9 cut(s) 8, 329, 686, 775, 1067, 1079, 1238, 1322, 1433
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 1 cut(s) 606
Hsp92II CATG 2 cut(s) 653, 1345
Kzo9I GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
Lsp1109I GCAGC 5 cut(s) 613, 1099, 1145, 1154, 1222
LweI GCATC 4 cut(s) 403, 496, 695, 768
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 4 cut(s) 553, 592, 671, 1149
MalI GATC 6 cut(s) 251, 452, 745, 993, 1020, 1123
MboI GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 2 cut(s) 450, 1121
MhlI GDGCHC 1 cut(s) 851
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 605
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 956
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 872, 1408
MspI CCGG 2 cut(s) 171, 639
MspR9I CCNGG 2 cut(s) 306, 929
MunI CAATTG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 306, 929
MvnI CGCG 1 cut(s) 742
NcoI CCATGG 1 cut(s) 1341
NdeII GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
NlaIII CATG 2 cut(s) 653, 1345
NlaIV GGNNCC 6 cut(s) 77, 115, 286, 303, 565, 1243
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 2 cut(s) 553, 592
NruI TCGCGA 1 cut(s) 742
NspV TTCGAA 2 cut(s) 349, 501
OliI CACNNNNGTG 1 cut(s) 956
PaqCI CACCTGC 1 cut(s) 671
PasI CCCWGGG 1 cut(s) 305
PceI AGGCCT 2 cut(s) 268, 1012
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 424
PkrI GCNGC 6 cut(s) 7, 628, 1114, 1135, 1144, 1237
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PpuMI RGGWCCY 2 cut(s) 285, 301
PshAI GACNNNNGTC 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 851
Psp5II RGGWCCY 2 cut(s) 285, 301
Psp6I CCWGG 2 cut(s) 304, 927
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 304, 927
PspN4I GGNNCC 6 cut(s) 77, 115, 286, 303, 565, 1243
PspPI GGNCC 6 cut(s) 113, 285, 301, 563, 578, 1242
PspPPI RGGWCCY 2 cut(s) 285, 301
PstI CTGCAG 1 cut(s) 1240
PsuI RGATCY 2 cut(s) 450, 1121
PvuII CAGCTG 1 cut(s) 872
RruI TCGCGA 1 cut(s) 742
RsaI GTAC 1 cut(s) 1033
RsaNI GTAC 1 cut(s) 1032
RseI CAYNNNNRTG 1 cut(s) 956
SacI GAGCTC 1 cut(s) 851
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 6 cut(s) 6, 627, 1113, 1134, 1143, 1236
Sau3AI GATC 6 cut(s) 249, 450, 743, 991, 1018, 1121
Sau96I GGNCC 6 cut(s) 113, 285, 301, 563, 578, 1242
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 306, 929
SduI GDGCHC 1 cut(s) 851
SfaNI GCATC 4 cut(s) 403, 496, 695, 768
SfcI CTRYAG 2 cut(s) 1014, 1236
SfuI TTCGAA 2 cut(s) 349, 501
SgrAI CRCCGGYG 1 cut(s) 638
SinI GGWCC 4 cut(s) 113, 285, 301, 578
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 4 cut(s) 1006, 1191, 1198, 1218
SmoI CTYRAG 4 cut(s) 1006, 1191, 1198, 1218
Sse9I AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
SseBI AGGCCT 2 cut(s) 268, 1012
SsiI CCGC 1 cut(s) 1406
SstI GAGCTC 1 cut(s) 851
StuI AGGCCT 2 cut(s) 268, 1012
StyD4I CCNGG 2 cut(s) 304, 927
StyI CCWWGG 2 cut(s) 1341, 1386
TaaI ACNGT 5 cut(s) 94, 313, 1330, 1372, 1534
TaiI ACGT 1 cut(s) 798
TaqI TCGA 4 cut(s) 349, 501, 570, 689
TaqII GACCGA 1 cut(s) 635
TasI AATT 8 cut(s) 22, 49, 196, 217, 227, 422, 558, 767
TatI WGTACW 1 cut(s) 1031
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 2 cut(s) 769, 1404
TseFI GTSAC 2 cut(s) 553, 592
TseI GCWGC 6 cut(s) 5, 626, 1112, 1133, 1142, 1235
Tsp45I GTSAC 2 cut(s) 553, 592
TspDTI ATGAA 5 cut(s) 15, 150, 267, 708, 799
TspGWI ACGGA 2 cut(s) 842, 897
TspRI CASTG 2 cut(s) 769, 1404
Van91I CCANNNNNTGG 1 cut(s) 424
VpaK11BI GGWCC 4 cut(s) 113, 285, 301, 578
XmiI GTMKAC 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.