Rh5BG042800

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0032734
Physical Location & Seq
Reverse (-)
3296132 .. 3301174
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG042800.1

Sequence Viewer

Length: 711 bp
ATGACATCATGGGTGTCCTCCAGTGACCCCTCTACCGGAGAATTTACTTTCAGTCTGGACCGGCCTGATCAAGCACCTCAGTTGGTGGTTAGGAAAGGGTCTGAGAAGCTAAATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCGAGGAGCTTCAGCCCAATCCGCTCTTGGACAATGGCAATCTGATCTTGAGAGATGAAAGTGGTGCAGATGCAGAGGGGTATGTGATTTGGCAGAGTTTTGATGACATAACAGACACTTTGTTGCCGGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACGTCATGGTTGTCCTCCAGTGACCCCTCTGCCGGAGAATTTACTTTCAGTGTGGAAAGGCCTGCTCAAGCACCTCAGTTGGTGGTTACGAAAAGGTCTGCGAAGCTATATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCAATGAGCTCAAGTCCAATCCGGTTTTCACTCCAATGTTCAATATAAGCAGTGAAGAGTTTATTGGTTGCTTAGAGTCTATTGCTGATGCTAAGAAGAAAAAGCGAGTGAAAGTGGCTGTCACCATTGTCATTTCATCACTTAGTGGGACGCTTATTTTTGGTGTTGTTGTCTGCATCATTAATCGGAATAGGAAGACAAAGAGGAGAGGTATTAAAGTTTATGAAATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

236

Amino Acids

26.31

Weight (kDa)

8.51

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 49 - 112 1.1e-15 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0032734)

Species Orthologous Gene IDs
rosa_samantha Rh5AG043200 Rh5BG042800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 332
AccBSI CCGCTC 1 cut(s) 175
AciI CCGC 1 cut(s) 173
AcsI RAATTY 4 cut(s) 41, 298, 365, 702
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 329
AdeI CACNNNGTG 1 cut(s) 620
AfiI CCNNNNNNNGG 4 cut(s) 35, 131, 359, 455
AgsI TTSAA 1 cut(s) 517
AjuI GAANNNNNNNTTGG 6 cut(s) 273, 305, 485, 517, 522, 554
AluBI AGCT 5 cut(s) 109, 160, 289, 433, 484
AluI AGCT 5 cut(s) 109, 160, 289, 433, 484
Alw21I GWGCWC 1 cut(s) 486
AoxI GGCC 4 cut(s) 62, 122, 386, 446
ApoI RAATTY 4 cut(s) 41, 298, 365, 702
AseI ATTAAT 1 cut(s) 657
AspS9I GGNCC 3 cut(s) 58, 122, 446
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 4 cut(s) 111, 125, 435, 449
BclI TGATCA 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 58, 122, 446
BmiI GGNNCC 2 cut(s) 123, 447
BmsI GCATC 3 cut(s) 211, 553, 660
BpiI GAAGAC 1 cut(s) 677
BpmI CTGGAG 2 cut(s) 4, 328
BpuEI CTTGAG 3 cut(s) 220, 378, 470
BsaHI GRCGYC 1 cut(s) 329
BsaJI CCNNGG 2 cut(s) 125, 449
BsaWI WCCGGW 3 cut(s) 35, 318, 496
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 131, 359, 455
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 2 cut(s) 21, 345
Bse3DI GCAATG 1 cut(s) 484
BseDI CCNNGG 2 cut(s) 125, 449
BseGI GGATG 2 cut(s) 136, 460
BseLI CCNNNNNNNGG 4 cut(s) 35, 131, 359, 455
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 92, 93, 416
BseNI ACTGG 2 cut(s) 21, 345
BseRI GAGGAG 2 cut(s) 170, 694
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 4 cut(s) 64, 124, 388, 448
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 6 cut(s) 36, 61, 278, 319, 360, 497
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 4 cut(s) 35, 131, 359, 455
BsmFI GGGAC 1 cut(s) 637
BsnI GGCC 4 cut(s) 64, 124, 388, 448
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 2 cut(s) 67, 195
BspACI CCGC 1 cut(s) 173
BspANI GGCC 4 cut(s) 64, 124, 388, 448
BspCNI CTCAG 3 cut(s) 91, 94, 415
BspLI GGNNCC 2 cut(s) 123, 447
BsrBI CCGCTC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 2 cut(s) 21, 345
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 125, 449
BssMI GATC 2 cut(s) 67, 195
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 2 cut(s) 125, 449
Bst6I CTCTTC 1 cut(s) 525
BstACI GRCGYC 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 6 cut(s) 78, 102, 402, 547, 567, 617
BstF5I GGATG 2 cut(s) 136, 460
BstKTI GATC 2 cut(s) 70, 198
BstMBI GATC 2 cut(s) 67, 195
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 677
BsuRI GGCC 4 cut(s) 64, 124, 388, 448
BtsCI GGATG 2 cut(s) 136, 460
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 6 cut(s) 28, 151, 352, 382, 475, 532
Cac8I GCNNGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 58, 122, 446
CseI GACGC 1 cut(s) 634
CviAII CATG 2 cut(s) 9, 333
DdeI CTNAG 6 cut(s) 78, 102, 402, 547, 567, 617
DpnI GATC 2 cut(s) 69, 197
DpnII GATC 2 cut(s) 67, 195
DraI TTTAAA 1 cut(s) 315
DraIII CACNNNGTG 1 cut(s) 620
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 125, 449
Eco147I AGGCCT 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 484
EcoO109I RGGNCCY 2 cut(s) 122, 446
EcoT14I CCWWGG 2 cut(s) 125, 449
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 2 cut(s) 125, 449
FaeI CATG 2 cut(s) 12, 336
FaiI YATR 9 cut(s) 10, 234, 260, 326, 334, 436, 438, 521, 699
FaqI GGGAC 1 cut(s) 637
FatI CATG 2 cut(s) 8, 332
FbaI TGATCA 1 cut(s) 67
FokI GGATG 2 cut(s) 143, 467
FriOI GRGCYC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 4, 328
HaeIII GGCC 4 cut(s) 64, 124, 388, 448
HapII CCGG 6 cut(s) 36, 61, 278, 319, 360, 497
HgaI GACGC 1 cut(s) 634
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 2 cut(s) 12, 336
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 5 cut(s) 135, 141, 459, 465, 551
HpaII CCGG 6 cut(s) 36, 61, 278, 319, 360, 497
HphI GGTGA 1 cut(s) 589
Hpy188I TCNGA 5 cut(s) 103, 140, 195, 464, 663
Hpy188III TCNNGA 2 cut(s) 56, 199
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 3 cut(s) 218, 224, 651
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 6 cut(s) 78, 102, 402, 547, 567, 617
HpySE526I ACGT 1 cut(s) 329
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 2 cut(s) 12, 336
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 2 cut(s) 67, 195
LmnI GCTCC 1 cut(s) 157
LweI GCATC 3 cut(s) 211, 553, 660
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 5 cut(s) 23, 304, 347, 412, 595
MalI GATC 2 cut(s) 69, 197
MbiI CCGCTC 1 cut(s) 175
MboI GATC 2 cut(s) 67, 195
MboII GAAGA 3 cut(s) 542, 583, 682
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 4 cut(s) 41, 298, 365, 702
MlyI GAGTC 3 cut(s) 144, 468, 560
MseI TTAA 4 cut(s) 314, 657, 690, 709
MslI CAYNNNNRTG 1 cut(s) 509
MspI CCGG 6 cut(s) 36, 61, 278, 319, 360, 497
MssI GTTTAAAC 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 2 cut(s) 67, 195
NlaIII CATG 2 cut(s) 12, 336
NlaIV GGNNCC 2 cut(s) 123, 447
NmuCI GTSAC 4 cut(s) 23, 304, 347, 595
PceI AGGCCT 1 cut(s) 388
PfeI GAWTC 2 cut(s) 141, 465
PleI GAGTC 3 cut(s) 143, 467, 559
PmeI GTTTAAAC 1 cut(s) 315
PpsI GAGTC 3 cut(s) 143, 467, 559
PshBI ATTAAT 1 cut(s) 657
Psp124BI GAGCTC 1 cut(s) 486
PspN4I GGNNCC 2 cut(s) 123, 447
PspPI GGNCC 3 cut(s) 58, 122, 446
RseI CAYNNNNRTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 4 cut(s) 314, 657, 690, 709
Sau3AI GATC 2 cut(s) 67, 195
Sau96I GGNCC 3 cut(s) 58, 122, 446
SchI GAGTC 3 cut(s) 144, 468, 560
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 3 cut(s) 211, 553, 660
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 3 cut(s) 199, 393, 485
SmoI CTYRAG 3 cut(s) 199, 393, 485
Sse9I AATT 4 cut(s) 41, 298, 365, 702
SseBI AGGCCT 1 cut(s) 388
SsiI CCGC 1 cut(s) 173
SstI GAGCTC 1 cut(s) 486
StuI AGGCCT 1 cut(s) 388
StyI CCWWGG 2 cut(s) 125, 449
TaiI ACGT 1 cut(s) 332
TasI AATT 4 cut(s) 41, 298, 365, 702
TfiI GAWTC 2 cut(s) 141, 465
Tru1I TTAA 4 cut(s) 314, 657, 690, 709
Tru9I TTAA 4 cut(s) 314, 657, 690, 709
TscAI CASTG 6 cut(s) 28, 151, 352, 382, 475, 532
TseFI GTSAC 4 cut(s) 23, 304, 347, 595
Tsp45I GTSAC 4 cut(s) 23, 304, 347, 595
TspDTI ATGAA 3 cut(s) 222, 299, 600
TspRI CASTG 6 cut(s) 28, 151, 352, 382, 475, 532
VpaK11BI GGWCC 1 cut(s) 58
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 4 cut(s) 41, 298, 365, 702
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
ZraI GACGTC 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.