Rh5BG378100

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0022197
Physical Location & Seq
Reverse (-)
59881450 .. 59885102
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG378100.1

Sequence Viewer

Length: 1191 bp
ATGGCCGACGATCGCGACGACCTCAGCGAATACTCGTCGGAGGACGAGGGCACCGAGGATTACAGGAAAGGAGGCTACCACGCCGTCCAGATTGGGGACTCCTTCAAGAATGGGCGCTACGTGGTTCAGACCAAGCTCGGCTGGGGTCACTTCTCTACCGTCTGGCTCGCTTGGGACACTCACACCTCGCGTTATGTTGCATTGAAGATTCAAAAGAGTGCTCGGATGTACACCGAAGCAGCAATGGATGAGATTAAGATTCTCAACCAGATTGCCGAGGGAGACCCCGGTGACAACAAGTTTGTTGTGAAGCTTTTGGATCATTTTAAGCATTTAGGTCCGAATGGGCAGCATGTCTGTATGGTTTTCGAGTACCTTGGGGATAATCTTTTGACTGTTATTAAGTATAGTGATTACAGAGGGACGCCGTTGCACATGGTTAAAGAGATTGCATTTCATATATTGGTGGGTTTGGATTACTTGCATCGTCAGCTTTCGATCATACACACTGATTTGAAGCCTGAGAATGTGTTGCTTTTGTCGATGATTGATCCATCTAAAGATCCGCGGAAGTCAGGTGTTCCTCTTGTCCTTCCAACGGGCAAAGATAAGACTGCGACGGTTTCTGGGGCTTCGAAAGATATTAAGAAGTCAAATGGGGATCTCACCAAGAATCAGAAAAAGAAGATTCGGAAAAAGGCTAAGAAGGCAACTCAGGGTTGTGGAGGGACTGAAACCTCTGAGGATGCTGAGGCAGCCTCCACCATAGTCAGTCCAGATGATTCTATTAATCAAGTGAAATCAAATGGGAAAGCTGTGGAAGTACAAGTGAATAATTCTGTGGTTAAAGATGAATCAACTACTGGTGAAGAAGTAAAAGACAATCACCAAGAAAATAAGGATCAGAAAAGACCAGACCCTTCAACAAGGAAGAAGCTGATGGCAGCTGCTGATCTCAAATGCAAATTGGTTGATTTTGGAAGTGCTTGTTGGACATATAAGCAATTCACAAGTGATATTCAGACAAGGCAGTACAGATGCCCAGAAGTTATTCTTGGAGATAAATACTCAACTCCAGCAGATATGTGGTCATTTGCTTGTGTTTGCTTTGAGCTAGCCACTGGTGATGTTCTTTTTGATCCACACAGCGGTGACAAATATGACAAAGATGAGGTAAATGCAGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

396

Amino Acids

44.18

Weight (kDa)

6.2

Isoelectric Point (pI)

21.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 180 1.5e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 42 - 180 2.7e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 317 - 390 8.7e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022197)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g30170
rosa_samantha Rh5BG378100 Rh5CG401400 Rh5DG391200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AccII CGCG 3 cut(s) 15, 190, 568
AciI CCGC 3 cut(s) 566, 568, 1149
AclWI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 4 cut(s) 230, 374, 825, 1034
AfiI CCNNNNNNNGG 3 cut(s) 94, 598, 1148
AgsI TTSAA 5 cut(s) 106, 205, 212, 517, 924
AjuI GAANNNNNNNTTGG 4 cut(s) 973, 1005, 1038, 1070
AleI CACNNNNGTG 1 cut(s) 1149
AluBI AGCT 7 cut(s) 136, 313, 493, 815, 937, 947, 1114
AluI AGCT 7 cut(s) 136, 313, 493, 815, 937, 947, 1114
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
AlwNI CAGNNNCTG 1 cut(s) 950
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 5 cut(s) 239, 349, 755, 944, 947
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 6 cut(s) 302, 658, 878, 878, 1136, 1163
AsuII TTCGAA 1 cut(s) 635
AsuNHI GCTAGC 1 cut(s) 1114
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 223
BbvCI CCTCAGC 2 cut(s) 23, 750
BbvI GCAGC 5 cut(s) 251, 361, 767, 934, 956
BccI CCATC 2 cut(s) 562, 934
BceAI ACGGC 2 cut(s) 68, 412
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 1115
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 5 cut(s) 240, 350, 756, 945, 948
BlsI GCNGC 5 cut(s) 241, 351, 757, 946, 949
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 288
BmsI GCATC 3 cut(s) 493, 736, 1028
BmtI GCTAGC 1 cut(s) 1118
BplI GAGNNNNNCTC 2 cut(s) 743, 775
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 2 cut(s) 23, 750
Bpu14I TTCGAA 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 5 cut(s) 54, 276, 286, 376, 566
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 598, 1148
Bse1I ACTGG 2 cut(s) 868, 1126
Bse3DI GCAATG 1 cut(s) 249
BseDI CCNNGG 5 cut(s) 54, 276, 286, 376, 566
BseGI GGATG 3 cut(s) 231, 253, 751
BseLI CCNNNNNNNGG 3 cut(s) 94, 598, 1148
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 5 cut(s) 37, 513, 728, 732, 741
BseNI ACTGG 2 cut(s) 868, 1126
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 5 cut(s) 251, 361, 767, 934, 956
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 3 cut(s) 15, 190, 568
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 50
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 4 cut(s) 110, 188, 436, 742
BslI CCNNNNNNNGG 3 cut(s) 94, 598, 1148
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 4 cut(s) 110, 188, 436, 742
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 53, 223
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
Bsp68I TCGCGA 1 cut(s) 15
BspACI CCGC 3 cut(s) 566, 568, 1149
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 36, 514, 727, 733, 742
BspFNI CGCG 3 cut(s) 15, 190, 568
BspLI GGNNCC 1 cut(s) 52
BspOI GCTAGC 1 cut(s) 1118
BspPI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 2 cut(s) 868, 1126
BssECI CCNNGG 5 cut(s) 54, 276, 286, 376, 566
BssMI GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 1 cut(s) 376
Bst4CI ACNGT 3 cut(s) 160, 397, 622
BstACI GRCGYC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 228
BstBAI YACGTR 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 168, 1116
BstDEI CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 3 cut(s) 231, 253, 751
BstFNI CGCG 3 cut(s) 15, 190, 568
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 9 cut(s) 13, 322, 501, 553, 565, 664, 904, 955, 1141
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 3 cut(s) 490, 707, 755
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 286
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 3 cut(s) 15, 190, 568
BstV1I GCAGC 5 cut(s) 251, 361, 767, 934, 956
BstX2I RGATCY 2 cut(s) 562, 661
BstYI RGATCY 2 cut(s) 562, 661
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 3 cut(s) 231, 253, 751
BtsIMutI CAGTG 2 cut(s) 507, 1119
BtuMI TCGCGA 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 168, 1116
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 338
Cfr42I CCGCGG 1 cut(s) 569
CseI GACGC 1 cut(s) 433
Csp6I GTAC 4 cut(s) 229, 373, 824, 1033
CviAII CATG 2 cut(s) 353, 436
CviQI GTAC 4 cut(s) 229, 373, 824, 1033
DdeI CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
DpnI GATC 9 cut(s) 12, 321, 500, 552, 564, 663, 903, 954, 1140
DpnII GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 2 cut(s) 356, 439
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 4 cut(s) 110, 188, 436, 742
FatI CATG 2 cut(s) 352, 435
Fnu4HI GCNGC 5 cut(s) 240, 350, 756, 945, 948
FokI GGATG 3 cut(s) 238, 260, 758
Fsp4HI GCNGC 5 cut(s) 240, 350, 756, 945, 948
FspBI CTAG 1 cut(s) 1115
GlaI GCGC 1 cut(s) 116
GluI GCNGC 5 cut(s) 240, 350, 756, 945, 948
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 1059
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 433
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 2 cut(s) 356, 439
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 7 cut(s) 98, 208, 259, 673, 688, 782, 854
HpaII CCGG 1 cut(s) 288
HphI GGTGA 6 cut(s) 302, 658, 878, 878, 1136, 1163
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 9 cut(s) 40, 129, 225, 342, 678, 693, 742, 906, 1023
Hpy188III TCNNGA 5 cut(s) 14, 88, 106, 776, 1188
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 4 cut(s) 11, 20, 40, 622
HpyAV CCTTC 4 cut(s) 112, 602, 700, 930
HpyCH4III ACNGT 3 cut(s) 160, 397, 622
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 6 cut(s) 200, 433, 452, 484, 963, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 490, 707, 755
HpyF3I CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 2 cut(s) 356, 439
HspAI GCGC 1 cut(s) 115
KspI CCGCGG 1 cut(s) 569
Kzo9I GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
Lsp1109I GCAGC 5 cut(s) 251, 361, 767, 934, 956
LweI GCATC 3 cut(s) 493, 736, 1028
MaeI CTAG 1 cut(s) 1115
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 146, 290, 1151
MalI GATC 9 cut(s) 12, 321, 500, 552, 564, 663, 903, 954, 1140
MboI GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
MboII GAAGA 4 cut(s) 217, 697, 881, 943
MflI RGATCY 2 cut(s) 562, 661
MhlI GDGCHC 2 cut(s) 53, 223
MluCI AATT 3 cut(s) 835, 965, 1004
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 3 cut(s) 18, 620, 971
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 7 cut(s) 255, 327, 402, 441, 645, 789, 846
MslI CAYNNNNRTG 1 cut(s) 1149
MspA1I CMGCKG 3 cut(s) 568, 947, 1149
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 288
MvnI CGCG 3 cut(s) 15, 190, 568
MwoI GCNNNNNNNGC 3 cut(s) 490, 707, 755
NciI CCSGG 1 cut(s) 288
NdeII GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
NheI GCTAGC 1 cut(s) 1114
NlaIII CATG 2 cut(s) 356, 439
NlaIV GGNNCC 1 cut(s) 52
NmeAIII GCCGAG 2 cut(s) 117, 301
NmuCI GTSAC 3 cut(s) 146, 290, 1151
NruI TCGCGA 1 cut(s) 15
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 635
OliI CACNNNNGTG 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 24, 51
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 6 cut(s) 208, 259, 673, 688, 782, 854
PkrI GCNGC 5 cut(s) 241, 351, 757, 946, 949
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 121
PshBI ATTAAT 1 cut(s) 789
PspFI CCCAGC 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 338
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 2 cut(s) 562, 661
PvuI CGATCG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 947
RruI TCGCGA 1 cut(s) 15
RsaI GTAC 4 cut(s) 230, 374, 825, 1034
RsaNI GTAC 4 cut(s) 229, 373, 824, 1033
RseI CAYNNNNRTG 1 cut(s) 1149
SacII CCGCGG 1 cut(s) 569
SaqAI TTAA 7 cut(s) 255, 327, 402, 441, 645, 789, 846
SatI GCNGC 5 cut(s) 240, 350, 756, 945, 948
Sau3AI GATC 9 cut(s) 10, 319, 498, 550, 562, 661, 901, 952, 1138
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 2 cut(s) 53, 223
SfaNI GCATC 3 cut(s) 493, 736, 1028
Sfr303I CCGCGG 1 cut(s) 569
SfuI TTCGAA 1 cut(s) 635
SgrBI CCGCGG 1 cut(s) 569
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 1149
Sse9I AATT 3 cut(s) 835, 965, 1004
SsiI CCGC 3 cut(s) 566, 568, 1149
SspMI CTAG 1 cut(s) 1115
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 3 cut(s) 160, 397, 622
TaiI ACGT 1 cut(s) 123
TaqI TCGA 4 cut(s) 369, 497, 542, 635
TasI AATT 3 cut(s) 835, 965, 1004
TatI WGTACW 3 cut(s) 228, 823, 1032
TfiI GAWTC 6 cut(s) 208, 259, 673, 688, 782, 854
Tru1I TTAA 7 cut(s) 255, 327, 402, 441, 645, 789, 846
Tru9I TTAA 7 cut(s) 255, 327, 402, 441, 645, 789, 846
TscAI CASTG 2 cut(s) 514, 1126
TseFI GTSAC 3 cut(s) 146, 290, 1151
TseI GCWGC 5 cut(s) 239, 349, 755, 944, 947
Tsp45I GTSAC 3 cut(s) 146, 290, 1151
TspDTI ATGAA 2 cut(s) 446, 867
TspRI CASTG 2 cut(s) 514, 1126
VpaK11BI GGWCC 1 cut(s) 338
VspI ATTAAT 1 cut(s) 789
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 1083
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 1 cut(s) 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.