Rh5DG391200

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0022197
Physical Location & Seq
Reverse (-)
61076814 .. 61080964
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG391200.1

Sequence Viewer

Length: 1254 bp
ATGGCCGACGACCGCGACGACCTCAGCGAATACTCGTCGGAGGACGAGGGCACCGAGGATTACAGGAAAGGAGGCTACCACGCCGTCCAGATTGGGGACTCCTTCAAGAATGGGCGCTACGTGGTTCAGACCAAGCTCGGCTGGGGTCACTTCTCCACCGTCTGGCTCGCTTGGGACACTCACACCTCGCGTTATGTTGCATTGAAGATTCAAAAGAGTGCTCGGATGTACACCGAAGCAGCAATGGATGAGATTAAGATTCTCAATCAGATTGCCGAGGGAGACCCCGGTGACAACAAGTTTGTTGTGAAGCTTTTGGATCATTTTAAGCATTTAGGTCCGAATGGGCAGCATGTTTGTATGGTTTTCGAGTACCTTGGGGATAATCTTTTGACTGTTATTAAGTATAGTGATTACCGGGGGACGCCGTTGCACATGGTTAAAGAGATTGCATTTCATATATTGGTGGGTTTGGATTACTTGCATCGTCAGCTTTCGATCATACACACGGATTTGAAGCCTGAGAATGTGTTGCTTTTGTCGATGATTGATCCATCTAAAGATCCGCGGAAGTCAGGTGTTCCTCTTGTCCTTCCAACGGGCAAAGATAAGACTGCGACGGTTTCTGGGGCTTCAAAAGATATTAAGAAGTTAAATGGGGATCTCACCAAGAATCAGAAAAAGAAGATTCGGAAAAAGGCTAAGAAGGCAACTCAGGGTTGTGGAGGGACTGAAACCTCTGAGGATGCTGAGGCAGACTCCACCATAGTCAGTCCAGATGATTCTATTAATGAAGTGAAATCAAATGGGAAAGCTGTGGAAGTACAAGTGAATAATTCTGTAGTTAAAGATGAATCAACTACCGGTGAAGAAGTAAAAGACAATCACCAAGAAAATAAGGATCAGAAAAGACCAGACCCTTCAACAAGGAAGAAGCTGATGGCAGCCGCTGACCTCAAATGCAAATTGGTTGATTTTGGAAGTGCTTGTTGGACATATAAACAATTCACAAGTGATATTCAGACAAGGCAGTACAGATGCCCAGAAGTTATCCTTGGAGATAAATACTCAACTCCAGCAGATATGTGGTCATTTGCTTGTGTTTGCTTTGAGCTAGCCACCGGTGATGTTCTTTTTGATCCACACAGCGGTGACAAATATGACAAAGATGAGGTAAGTGCAGTATTTGATTATCCCCTTTATAATTGTTACAGAAATTTTGACCGTCTGCAGTACTTCATTGCCTGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

417

Amino Acids

47.08

Weight (kDa)

5.98

Isoelectric Point (pI)

21.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 180 1.7e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 42 - 179 3.7e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 317 - 393 5.2e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022197)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g30170
rosa_samantha Rh5BG378100 Rh5CG401400 Rh5DG391200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1203
AccB1I GGYRCC 1 cut(s) 50
AccB7I CCANNNNNTGG 1 cut(s) 162
AccII CGCG 3 cut(s) 15, 190, 568
AciI CCGC 5 cut(s) 13, 566, 568, 948, 1149
AclWI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1216
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 5 cut(s) 230, 374, 825, 1034, 1235
AfiI CCNNNNNNNGG 4 cut(s) 94, 162, 598, 1148
AgeI ACCGGT 2 cut(s) 863, 1121
AgsI TTSAA 6 cut(s) 106, 205, 212, 517, 636, 924
AjnI CCWGG 1 cut(s) 1244
AjuI GAANNNNNNNTTGG 4 cut(s) 973, 1005, 1038, 1070
AleI CACNNNNGTG 1 cut(s) 1149
AluBI AGCT 6 cut(s) 136, 313, 493, 815, 937, 1114
AluI AGCT 6 cut(s) 136, 313, 493, 815, 937, 1114
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
AlwNI CAGNNNCTG 1 cut(s) 950
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 239, 349, 944
ApoI RAATTY 1 cut(s) 1216
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AseI ATTAAT 1 cut(s) 789
AsiGI ACCGGT 2 cut(s) 863, 1121
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 2 cut(s) 288, 419
AsuHPI GGTGA 6 cut(s) 302, 658, 878, 878, 1136, 1163
AsuNHI GCTAGC 1 cut(s) 1114
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 223
BbvCI CCTCAGC 2 cut(s) 23, 750
BbvI GCAGC 3 cut(s) 251, 361, 956
BccI CCATC 2 cut(s) 562, 934
BceAI ACGGC 2 cut(s) 68, 412
BciT130I CCWGG 1 cut(s) 1246
BcnI CCSGG 2 cut(s) 288, 419
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 1115
BfmI CTRYAG 2 cut(s) 840, 1229
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 4 cut(s) 240, 350, 945, 948
BlsI GCNGC 4 cut(s) 241, 351, 946, 949
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 3 cut(s) 288, 419, 1246
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 3 cut(s) 288, 419, 1246
BmsI GCATC 3 cut(s) 493, 736, 1028
BmtI GCTAGC 1 cut(s) 1118
BplI GAGNNNNNCTC 2 cut(s) 743, 775
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 2 cut(s) 23, 750
BpuMI CCSGG 2 cut(s) 288, 419
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 7 cut(s) 54, 276, 286, 376, 418, 566, 1054
BsaWI WCCGGW 2 cut(s) 863, 1121
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 162, 598, 1148
Bse118I RCCGGY 2 cut(s) 863, 1121
Bse3DI GCAATG 2 cut(s) 249, 1239
BseBI CCWGG 1 cut(s) 1246
BseDI CCNNGG 7 cut(s) 54, 276, 286, 376, 418, 566, 1054
BseGI GGATG 3 cut(s) 231, 253, 751
BseLI CCNNNNNNNGG 4 cut(s) 94, 162, 598, 1148
BseMI GCAATG 2 cut(s) 249, 1239
BseMII CTCAG 5 cut(s) 37, 513, 728, 732, 741
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 3 cut(s) 251, 361, 956
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 3 cut(s) 15, 190, 568
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 50
BshTI ACCGGT 2 cut(s) 863, 1121
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 4 cut(s) 288, 418, 864, 1122
BslFI GGGAC 4 cut(s) 110, 188, 436, 742
BslI CCNNNNNNNGG 4 cut(s) 94, 162, 598, 1148
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 4 cut(s) 110, 188, 436, 742
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 53, 223
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
BspACI CCGC 5 cut(s) 13, 566, 568, 948, 1149
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 36, 514, 727, 733, 742
BspFNI CGCG 3 cut(s) 15, 190, 568
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 1233
BspOI GCTAGC 1 cut(s) 1118
BspPI GGATC 6 cut(s) 327, 545, 557, 669, 909, 1133
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 2 cut(s) 249, 1239
BsrFI RCCGGY 2 cut(s) 863, 1121
BsrGI TGTACA 1 cut(s) 228
BssAI RCCGGY 2 cut(s) 863, 1121
BssECI CCNNGG 7 cut(s) 54, 276, 286, 376, 418, 566, 1054
BssMI GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 376, 1054
Bst2UI CCWGG 1 cut(s) 1246
Bst4CI ACNGT 4 cut(s) 160, 397, 622, 1226
BstACI GRCGYC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 228
BstBAI YACGTR 1 cut(s) 121
BstC8I GCNNGC 2 cut(s) 168, 1116
BstDEI CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 3 cut(s) 231, 253, 751
BstFNI CGCG 3 cut(s) 15, 190, 568
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 7 cut(s) 322, 501, 553, 565, 664, 904, 1141
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 490, 707
BstNI CCWGG 1 cut(s) 1246
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 286, 417, 1244
BstSFI CTRYAG 2 cut(s) 840, 1229
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 3 cut(s) 15, 190, 568
BstV1I GCAGC 3 cut(s) 251, 361, 956
BstX2I RGATCY 2 cut(s) 562, 661
BstYI RGATCY 2 cut(s) 562, 661
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 3 cut(s) 231, 253, 751
Cac8I GCNNGC 2 cut(s) 168, 1116
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 2 cut(s) 863, 1121
Cfr13I GGNCC 1 cut(s) 338
Cfr42I CCGCGG 1 cut(s) 569
CseI GACGC 1 cut(s) 433
Csp6I GTAC 5 cut(s) 229, 373, 824, 1033, 1234
CspAI ACCGGT 2 cut(s) 863, 1121
CviAII CATG 2 cut(s) 353, 436
CviQI GTAC 5 cut(s) 229, 373, 824, 1033, 1234
DdeI CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
DpnI GATC 7 cut(s) 321, 500, 552, 564, 663, 903, 1140
DpnII GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 376, 1054
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 1244
EcoT14I CCWWGG 2 cut(s) 376, 1054
ErhI CCWWGG 2 cut(s) 376, 1054
FaeI CATG 2 cut(s) 356, 439
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 4 cut(s) 110, 188, 436, 742
FatI CATG 2 cut(s) 352, 435
Fnu4HI GCNGC 4 cut(s) 240, 350, 945, 948
FokI GGATG 3 cut(s) 238, 260, 758
Fsp4HI GCNGC 4 cut(s) 240, 350, 945, 948
FspBI CTAG 1 cut(s) 1115
GlaI GCGC 1 cut(s) 116
GluI GCNGC 4 cut(s) 240, 350, 945, 948
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 1059
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 288, 418, 864, 1122
HgaI GACGC 1 cut(s) 433
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 2 cut(s) 356, 439
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 8 cut(s) 98, 208, 259, 673, 688, 758, 782, 854
HpaII CCGG 4 cut(s) 288, 418, 864, 1122
HphI GGTGA 6 cut(s) 302, 658, 878, 878, 1136, 1163
Hpy166II GTNNAC 1 cut(s) 231
Hpy188III TCNNGA 3 cut(s) 88, 106, 776
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 4 cut(s) 11, 20, 40, 622
HpyAV CCTTC 4 cut(s) 112, 602, 700, 930
HpyCH4III ACNGT 4 cut(s) 160, 397, 622, 1226
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 7 cut(s) 200, 433, 452, 484, 963, 1181, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 490, 707
HpyF3I CTNAG 6 cut(s) 23, 522, 702, 714, 741, 750
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 2 cut(s) 356, 439
HspAI GCGC 1 cut(s) 115
KspI CCGCGG 1 cut(s) 569
Kzo9I GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
Lsp1109I GCAGC 3 cut(s) 251, 361, 956
LweI GCATC 3 cut(s) 493, 736, 1028
MaeI CTAG 1 cut(s) 1115
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 4 cut(s) 146, 290, 1151, 1208
MalI GATC 7 cut(s) 321, 500, 552, 564, 663, 903, 1140
MboI GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
MboII GAAGA 4 cut(s) 217, 697, 881, 943
MflI RGATCY 2 cut(s) 562, 661
MhlI GDGCHC 2 cut(s) 53, 223
MluCI AATT 5 cut(s) 835, 965, 1004, 1204, 1216
MlyI GAGTC 2 cut(s) 92, 752
MmeI TCCRAC 3 cut(s) 18, 620, 971
MseI TTAA 8 cut(s) 255, 327, 402, 441, 645, 653, 789, 846
MslI CAYNNNNRTG 1 cut(s) 1149
MspA1I CMGCKG 3 cut(s) 568, 950, 1149
MspI CCGG 4 cut(s) 288, 418, 864, 1122
MspR9I CCNGG 3 cut(s) 288, 419, 1246
MvaI CCWGG 1 cut(s) 1246
MvnI CGCG 3 cut(s) 15, 190, 568
MwoI GCNNNNNNNGC 2 cut(s) 490, 707
NciI CCSGG 2 cut(s) 288, 419
NdeII GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
NheI GCTAGC 1 cut(s) 1114
NlaIII CATG 2 cut(s) 356, 439
NlaIV GGNNCC 1 cut(s) 52
NmeAIII GCCGAG 2 cut(s) 117, 301
NmuCI GTSAC 3 cut(s) 146, 290, 1151
NspI RCATGY 1 cut(s) 356
OliI CACNNNNGTG 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 24, 51
PfeI GAWTC 6 cut(s) 208, 259, 673, 688, 782, 854
PflMI CCANNNNNTGG 1 cut(s) 162
PinAI ACCGGT 2 cut(s) 863, 1121
PkrI GCNGC 4 cut(s) 241, 351, 946, 949
PleI GAGTC 2 cut(s) 92, 752
PpsI GAGTC 2 cut(s) 92, 752
Ppu21I YACGTR 1 cut(s) 121
PshBI ATTAAT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 1203
Psp6I CCWGG 1 cut(s) 1244
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 1244
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 338
PstI CTGCAG 1 cut(s) 1233
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 2 cut(s) 562, 661
RsaI GTAC 5 cut(s) 230, 374, 825, 1034, 1235
RsaNI GTAC 5 cut(s) 229, 373, 824, 1033, 1234
RseI CAYNNNNRTG 1 cut(s) 1149
SacII CCGCGG 1 cut(s) 569
SaqAI TTAA 8 cut(s) 255, 327, 402, 441, 645, 653, 789, 846
SatI GCNGC 4 cut(s) 240, 350, 945, 948
Sau3AI GATC 7 cut(s) 319, 498, 550, 562, 661, 901, 1138
Sau96I GGNCC 1 cut(s) 338
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 2 cut(s) 92, 752
ScrFI CCNGG 3 cut(s) 288, 419, 1246
SduI GDGCHC 2 cut(s) 53, 223
SfaNI GCATC 3 cut(s) 493, 736, 1028
SfcI CTRYAG 2 cut(s) 840, 1229
Sfr303I CCGCGG 1 cut(s) 569
SgrAI CRCCGGYG 1 cut(s) 1121
SgrBI CCGCGG 1 cut(s) 569
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 1149
Sse9I AATT 5 cut(s) 835, 965, 1004, 1204, 1216
SsiI CCGC 5 cut(s) 13, 566, 568, 948, 1149
SspMI CTAG 1 cut(s) 1115
StyD4I CCNGG 3 cut(s) 286, 417, 1244
StyI CCWWGG 2 cut(s) 376, 1054
TaaI ACNGT 4 cut(s) 160, 397, 622, 1226
TaiI ACGT 1 cut(s) 123
TaqI TCGA 3 cut(s) 369, 497, 542
TasI AATT 5 cut(s) 835, 965, 1004, 1204, 1216
TatI WGTACW 4 cut(s) 228, 823, 1032, 1233
TauI GCSGC 1 cut(s) 950
TfiI GAWTC 6 cut(s) 208, 259, 673, 688, 782, 854
Tru1I TTAA 8 cut(s) 255, 327, 402, 441, 645, 653, 789, 846
Tru9I TTAA 8 cut(s) 255, 327, 402, 441, 645, 653, 789, 846
TseFI GTSAC 3 cut(s) 146, 290, 1151
TseI GCWGC 3 cut(s) 239, 349, 944
Tsp45I GTSAC 3 cut(s) 146, 290, 1151
TspDTI ATGAA 4 cut(s) 446, 807, 867, 1228
TspGWI ACGGA 1 cut(s) 524
Van91I CCANNNNNTGG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 338
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 1 cut(s) 1216
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 1083
XspI CTAG 1 cut(s) 1115
ZrmI AGTACT 1 cut(s) 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.