Rh5BG454400

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0025485
Physical Location & Seq
Reverse (-)
72874864 .. 72875229
366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG454400.1

Sequence Viewer

Length: 366 bp
ATGACGACGATGTTAACCAACCCCAACACCAGTCCCAGTTCCAATCCCAAAGAACAAACTAATTCCCGATCACCCAAAAGTCAGCGATGGCGGCAGAGCCAGGTGGCGACGGCCGTGATAGCCATCGCAGTGCTCTTGATCTCCACCGCCGTGTGGCGCTCTTTGGTGTTCTTCGGCAGTTCTCCATCGCTTATGGTGTCCGAGGACATCTCTAGGTTTCAGTACACAAACCCGACATGTCACCTATCGGGGATCCGAATCTCTCGGATAATTTTCGAATGCTTCTGCCTCATGCCCAAAAGCCGGTGGTTTGTGCTCGCAGATGACGACACCATCATCAACGCTGATAACCTCGTCGAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

121

Amino Acids

13.65

Weight (kDa)

8.59

Isoelectric Point (pI)

64.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025485)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000605.1_g000047
rosa_samantha Rh5BG454400 Rh5CG476700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 91, 147
AclWI GGATC 2 cut(s) 247, 260
AcoI YGGCCR 1 cut(s) 111
AfaI GTAC 2 cut(s) 224, 362
AfiI CCNNNNNNNGG 2 cut(s) 153, 303
AflIII ACRYGT 1 cut(s) 236
AjnI CCWGG 1 cut(s) 99
AleI CACNNNNGTG 1 cut(s) 149
Alw21I GWGCWC 2 cut(s) 135, 318
AlwI GGATC 2 cut(s) 247, 260
AoxI GGCC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 159
AsuHPI GGTGA 2 cut(s) 63, 233
AsuII TTCGAA 1 cut(s) 276
BamHI GGATCC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 135, 318
BccI CCATC 4 cut(s) 81, 131, 193, 341
BceAI ACGGC 3 cut(s) 98, 126, 134
BciT130I CCWGG 1 cut(s) 101
BfaI CTAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 160
BisI GCNGC 1 cut(s) 92
BlsI GCNGC 1 cut(s) 93
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 101
BmrI ACTGGG 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 194, 226
Bpu14I TTCGAA 1 cut(s) 276
BsaBI GATNNNNATC 2 cut(s) 122, 257
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 303
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 2 cut(s) 30, 36
Bse8I GATNNNNATC 2 cut(s) 122, 257
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 201
BseJI GATNNNNATC 2 cut(s) 122, 257
BseLI CCNNNNNNNGG 2 cut(s) 153, 303
BseNI ACTGG 2 cut(s) 30, 36
BseX3I CGGCCG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 2 cut(s) 135, 318
BsiSI CCGG 1 cut(s) 304
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 153, 303
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 135, 318
Bsp143I GATC 3 cut(s) 68, 138, 252
BspACI CCGC 2 cut(s) 91, 147
BspANI GGCC 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 254
BspPI GGATC 2 cut(s) 247, 260
BspT104I TTCGAA 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 2 cut(s) 30, 36
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 3 cut(s) 68, 138, 252
Bst2UI CCWGG 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 3 cut(s) 71, 141, 255
BstMBI GATC 3 cut(s) 68, 138, 252
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 91, 119
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 111
BsuRI GGCC 1 cut(s) 113
BtgZI GCGATG 3 cut(s) 100, 109, 171
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 303
Csp6I GTAC 2 cut(s) 223, 361
CviAII CATG 2 cut(s) 237, 292
CviJI RGCY 4 cut(s) 99, 113, 122, 303
CviKI_1 RGCY 4 cut(s) 99, 113, 122, 303
CviQI GTAC 2 cut(s) 223, 361
DpnI GATC 3 cut(s) 70, 140, 254
DpnII GATC 3 cut(s) 68, 138, 252
EaeI YGGCCR 1 cut(s) 111
EagI CGGCCG 1 cut(s) 111
EclXI CGGCCG 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 99
FaeI CATG 2 cut(s) 240, 295
FaiI YATR 3 cut(s) 194, 238, 293
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 236, 291
Fnu4HI GCNGC 1 cut(s) 92
Fsp4HI GCNGC 1 cut(s) 92
FspBI CTAG 1 cut(s) 213
GlaI GCGC 1 cut(s) 158
GluI GCNGC 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 1 cut(s) 113
HapII CCGG 1 cut(s) 304
HhaI GCGC 1 cut(s) 159
Hin1II CATG 2 cut(s) 240, 295
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 1 cut(s) 258
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 1 cut(s) 304
HphI GGTGA 2 cut(s) 63, 233
Hpy166II GTNNAC 2 cut(s) 15, 225
Hpy188I TCNGA 3 cut(s) 202, 257, 267
Hpy188III TCNNGA 2 cut(s) 66, 136
Hpy8I GTNNAC 2 cut(s) 15, 225
Hpy99I CGWCG 3 cut(s) 10, 112, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 119
Hsp92II CATG 2 cut(s) 240, 295
HspAI GCGC 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 3 cut(s) 68, 138, 252
LpnPI CCDG 5 cut(s) 43, 49, 86, 113, 317
MaeI CTAG 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 70, 140, 254
MboI GATC 3 cut(s) 68, 138, 252
MboII GAAGA 1 cut(s) 163
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 2 cut(s) 135, 318
MluCI AATT 2 cut(s) 61, 270
MnlI CCTC 3 cut(s) 196, 299, 362
MseI TTAA 1 cut(s) 14
MslI CAYNNNNRTG 2 cut(s) 128, 149
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 91, 119
NdeII GATC 3 cut(s) 68, 138, 252
NlaIII CATG 2 cut(s) 240, 295
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 239
NspI RCATGY 1 cut(s) 240
NspV TTCGAA 1 cut(s) 276
OliI CACNNNNGTG 1 cut(s) 149
PciI ACATGT 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 93
PscI ACATGT 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 254
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 2 cut(s) 224, 362
RsaNI GTAC 2 cut(s) 223, 361
RseI CAYNNNNRTG 2 cut(s) 128, 149
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 1 cut(s) 92
Sau3AI GATC 3 cut(s) 68, 138, 252
ScaI AGTACT 1 cut(s) 362
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 2 cut(s) 135, 318
SetI ASST 4 cut(s) 105, 218, 246, 354
SfuI TTCGAA 1 cut(s) 276
SmiMI CAYNNNNRTG 2 cut(s) 128, 149
Sse9I AATT 2 cut(s) 61, 270
SsiI CCGC 2 cut(s) 91, 147
SspMI CTAG 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 99
TaqI TCGA 2 cut(s) 276, 357
TasI AATT 2 cut(s) 61, 270
TatI WGTACW 2 cut(s) 222, 360
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 1 cut(s) 135
TseFI GTSAC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 239
TspRI CASTG 1 cut(s) 135
XceI RCATGY 1 cut(s) 240
XspI CTAG 1 cut(s) 213
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.