Rh5BG502400

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020137
Physical Location & Seq
Reverse (-)
79883839 .. 79886454
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG502400.1

Sequence Viewer

Length: 447 bp
ATGGCGTCGAAACGAATCTTGAAGGAGCTCAAGGATTTGCAGAAGGACCCGCCTACCTCTTGCAGTGCTGGTCCCGTGGCGGAGGACATGTTCCATTGGCAAGCAACAATTATGGGACCTGCTGATAGCCCATATGCAGGTGGTGTTTTTCTAGTTTCAATTCACTTCCCTCCGGATTATCCATTCAAGCCTCCAAAGGTTGCATTCAGGACCAAGGTATTCCATCCAAACATCAATAGCAATGGAAGCATATGTCTTGATATTCTTAAGGAACAATGGAGCCCAGCACTAACCATTTCCAAGGTGCTGTTATCTATTTGCTCTCTGTTGACAGATCCCAACCCTGATGATCCCCTTGTCCCCGAAATTGCACACATGTACAAGACCGACAGGGCAAAGTATGAAGCCACTGCTCGCAGCTGGACACAAAAGTATGCCATGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

148

Amino Acids

16.46

Weight (kDa)

7.72

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 5.8e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020137)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0073541
rosa_multiflora Rmu_ssc0000226.1_g000007
rosa_roxburghii Rroxscaffold_1G00007730
rosa_rugosa Rorug05G0424600
rosa_samantha Rh5BG502400
rosa_wichuraiana Rw5G044750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 2 cut(s) 127, 128
AccIII TCCGGA 1 cut(s) 172
AciI CCGC 2 cut(s) 50, 80
AclWI GGATC 2 cut(s) 329, 344
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 137
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 87, 375
AgsI TTSAA 3 cut(s) 22, 159, 187
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw21I GWGCWC 1 cut(s) 30
AlwI GGATC 2 cut(s) 329, 344
Aor13HI TCCGGA 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 46, 71, 116, 210
AvaII GGWCC 4 cut(s) 46, 71, 116, 210
BanII GRGCYC 2 cut(s) 30, 284
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 231
BfaI CTAG 1 cut(s) 152
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 127, 128
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme18I GGWCC 4 cut(s) 46, 71, 116, 210
BmgT120I GGNCC 4 cut(s) 46, 71, 116, 210
BmiI GGNNCC 4 cut(s) 48, 73, 117, 281
BpuEI CTTGAG 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 75, 213, 300, 438
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 247
BseAI TCCGGA 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 75, 213, 300, 438
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 3 cut(s) 57, 129, 344
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 3 cut(s) 57, 129, 344
BsmI GAATGC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 30, 284
Bsp13I TCCGGA 1 cut(s) 172
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 2 cut(s) 334, 349
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 2 cut(s) 50, 80
BspEI TCCGGA 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 48, 73, 117, 281
BspMI ACCTGC 2 cut(s) 127, 128
BspPI GGATC 2 cut(s) 329, 344
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 4 cut(s) 75, 213, 300, 438
BssMI GATC 2 cut(s) 334, 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 213, 300, 438
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 102, 415
BstDSI CCRYGG 2 cut(s) 75, 438
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 2 cut(s) 337, 352
BstMBI GATC 2 cut(s) 334, 349
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 91, 379
BstV1I GCAGC 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BtgI CCRYGG 2 cut(s) 75, 438
BtsCI GGATG 1 cut(s) 223
BtsI GCAGTG 2 cut(s) 70, 408
BtsIMutI CAGTG 2 cut(s) 70, 408
BveI ACCTGC 2 cut(s) 127, 128
Cac8I GCNNGC 2 cut(s) 102, 415
Cfr13I GGNCC 4 cut(s) 46, 71, 116, 210
Csp6I GTAC 1 cut(s) 379
CviAII CATG 3 cut(s) 88, 376, 439
CviJI RGCY 7 cut(s) 28, 129, 190, 282, 407, 420, 444
CviKI_1 RGCY 7 cut(s) 28, 129, 190, 282, 407, 420, 444
CviQI GTAC 1 cut(s) 379
DpnI GATC 2 cut(s) 336, 351
DpnII GATC 2 cut(s) 334, 349
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 213, 300, 438
Eco24I GRGCYC 2 cut(s) 30, 284
Eco47I GGWCC 4 cut(s) 46, 71, 116, 210
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 2 cut(s) 46, 116
EcoT14I CCWWGG 3 cut(s) 213, 300, 438
EcoT38I GRGCYC 2 cut(s) 30, 284
ErhI CCWWGG 3 cut(s) 213, 300, 438
FaeI CATG 3 cut(s) 91, 379, 442
FaqI GGGAC 3 cut(s) 57, 129, 344
FatI CATG 3 cut(s) 87, 375, 438
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 2 cut(s) 133, 251
Fnu4HI GCNGC 1 cut(s) 418
FokI GGATG 1 cut(s) 210
FriOI GRGCYC 2 cut(s) 30, 284
Fsp4HI GCNGC 1 cut(s) 418
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 418
GsaI CCCAGC 1 cut(s) 287
HapII CCGG 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 91, 379, 442
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 15
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 330
Hpy188III TCNNGA 4 cut(s) 19, 173, 208, 257
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 16, 37
HpyCH4V TGCA 5 cut(s) 40, 63, 137, 203, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 91, 379, 442
Kpn2I TCCGGA 1 cut(s) 172
Kzo9I GATC 2 cut(s) 334, 349
LmnI GCTCC 2 cut(s) 25, 279
LpnPI CCDG 9 cut(s) 54, 123, 132, 186, 193, 297, 357, 376, 406
Lsp1109I GCAGC 1 cut(s) 429
MaeI CTAG 1 cut(s) 152
MalI GATC 2 cut(s) 336, 351
MboI GATC 2 cut(s) 334, 349
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 30, 284
MluCI AATT 3 cut(s) 108, 159, 366
MnlI CCTC 4 cut(s) 67, 76, 180, 201
MroI TCCGGA 1 cut(s) 172
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 420
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 246
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 2 cut(s) 133, 251
NdeII GATC 2 cut(s) 334, 349
NlaIII CATG 3 cut(s) 91, 379, 442
NlaIV GGNNCC 4 cut(s) 48, 73, 117, 281
NspI RCATGY 2 cut(s) 91, 379
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 2 cut(s) 87, 375
PctI GAATGC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 419
PpuMI RGGWCCY 2 cut(s) 46, 116
PscI ACATGT 2 cut(s) 87, 375
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 2 cut(s) 46, 116
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 4 cut(s) 48, 73, 117, 281
PspPI GGNCC 4 cut(s) 46, 71, 116, 210
PspPPI RGGWCCY 2 cut(s) 46, 116
PsuI RGATCY 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 420
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 2 cut(s) 334, 349
Sau96I GGNCC 4 cut(s) 46, 71, 116, 210
SduI GDGCHC 2 cut(s) 30, 284
SetI ASST 8 cut(s) 30, 59, 121, 142, 201, 219, 306, 422
SinI GGWCC 4 cut(s) 46, 71, 116, 210
SmlI CTYRAG 2 cut(s) 29, 266
SmoI CTYRAG 2 cut(s) 29, 266
Sse9I AATT 3 cut(s) 108, 159, 366
SsiI CCGC 2 cut(s) 50, 80
SspMI CTAG 1 cut(s) 152
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 3 cut(s) 213, 300, 438
TaqI TCGA 1 cut(s) 8
TaqII GACCGA 1 cut(s) 401
TasI AATT 3 cut(s) 108, 159, 366
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 70, 415
TseI GCWGC 1 cut(s) 417
TspDTI ATGAA 1 cut(s) 417
TspRI CASTG 2 cut(s) 70, 415
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 4 cut(s) 46, 71, 116, 210
XceI RCATGY 2 cut(s) 91, 379
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.