Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0022990

Physical Location & Seq

Genomic Coordinates Reverse (-)

83071166 .. 83078255

Gene Structure 7090 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG527100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 465 bp

ATGAAGATGGTTGATGTTATCACAACACCAATTCCCATATTGATGCCTTCTCGTCTGAACCTAAAATTGGGTTATTCCTCTGGTGGTGGTTTGTCAACATATGATCCAGCACTTGATTTTTTGAGACACATGGAGGAGAGTGAGAGAGATCGGCTTGAATACCTACAGAAGGTTGCGCAGAAGGCCTGTTCGCAGGATCCTGAGGTTGTAGCTTATGCCCGTTTTGCCGTGGAAGAGTTCAACAAGAGAGAGAATGCACAACTGCAATTTGTGAGAGGGCTATGTCCAGCATGTGTAGATTGTGATGGTCGTGTTTATTTGCTAAAGCTAGAAGCACTTGATGCTGATCATGGGCTCAAAACTTACCAAACTAAAGTTAAGGTCTTTCCAAATGGCAAAAATCCGCCGACTCTCCGTTTCTGTGGTCTATGGGATTTTAATGAACCTGACTCTGTCCAAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

154

Amino Acids

17.39

Weight (kDa)

5.71

Isoelectric Point (pI)

49.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQAPI	PF16845	65 - 126	1.8e-07	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022990)

Species	Orthologous Gene IDs
rosa_laevigata	RLG00000036645
rosa_multiflora	Rmu_sc0003323.1_g000021
rosa_samantha	Rh5BG527100 Rh5CG551200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	177
AciI	CCGC	1 cut(s)	404
AclWI	GGATC	3 cut(s)	98, 191, 204
AfiI	CCNNNNNNNGG	2 cut(s)	67, 169
AgsI	TTSAA	2 cut(s)	158, 241
AjuI	GAANNNNNNNTTGG	2 cut(s)	50, 82
AluBI	AGCT	2 cut(s)	212, 328
AluI	AGCT	2 cut(s)	212, 328
Alw26I	GTCTC	1 cut(s)	118
AlwI	GGATC	3 cut(s)	98, 191, 204
AoxI	GGCC	1 cut(s)	183
AspLEI	GCGC	1 cut(s)	178
AxyI	CCTNAGG	1 cut(s)	201
BamHI	GGATCC	1 cut(s)	196
BanII	GRGCYC	1 cut(s)	357
BccI	CCATC	1 cut(s)	299
BceAI	ACGGC	1 cut(s)	212
BclI	TGATCA	1 cut(s)	346
BcoDI	GTCTC	1 cut(s)	118
BfaI	CTAG	1 cut(s)	329
BfmI	CTRYAG	1 cut(s)	164
BmiI	GGNNCC	1 cut(s)	198
BmsI	GCATC	2 cut(s)	33, 331
BsaBI	GATNNNNATC	1 cut(s)	345
BsaJI	CCNNGG	2 cut(s)	228, 457
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 169
Bse21I	CCTNAGG	1 cut(s)	201
Bse8I	GATNNNNATC	1 cut(s)	345
BseDI	CCNNGG	2 cut(s)	228, 457
BseJI	GATNNNNATC	1 cut(s)	345
BseLI	CCNNNNNNNGG	2 cut(s)	67, 169
BseMII	CTCAG	1 cut(s)	192
BseRI	GAGGAG	1 cut(s)	149
BshFI	GGCC	1 cut(s)	185
BslI	CCNNNNNNNGG	2 cut(s)	67, 169
BsmAI	GTCTC	1 cut(s)	118
BsmI	GAATGC	1 cut(s)	259
BsnI	GGCC	1 cut(s)	185
Bsp1286I	GDGCHC	1 cut(s)	357
Bsp143I	GATC	4 cut(s)	103, 148, 196, 346
BspACI	CCGC	1 cut(s)	404
BspANI	GGCC	1 cut(s)	185
BspCNI	CTCAG	1 cut(s)	193
BspLI	GGNNCC	1 cut(s)	198
BspPI	GGATC	3 cut(s)	98, 191, 204
BssECI	CCNNGG	2 cut(s)	228, 457
BssMI	GATC	4 cut(s)	103, 148, 196, 346
BssT1I	CCWWGG	1 cut(s)	457
Bst6I	CTCTTC	1 cut(s)	228
BstAPI	GCANNNNNTGC	1 cut(s)	341
BstDEI	CTNAG	1 cut(s)	201
BstDSI	CCRYGG	1 cut(s)	228
BstENI	CCTNNNNNAGG	1 cut(s)	167
BstHHI	GCGC	1 cut(s)	178
BstKTI	GATC	4 cut(s)	106, 151, 199, 349
BstMAI	GTCTC	1 cut(s)	118
BstMBI	GATC	4 cut(s)	103, 148, 196, 346
BstMWI	GCNNNNNNNGC	3 cut(s)	182, 224, 341
BstNSI	RCATGY	1 cut(s)	294
BstSFI	CTRYAG	1 cut(s)	164
BstX2I	RGATCY	1 cut(s)	196
BstYI	RGATCY	1 cut(s)	196
Bsu36I	CCTNAGG	1 cut(s)	201
BsuRI	GGCC	1 cut(s)	185
BtgI	CCRYGG	1 cut(s)	228
CfoI	GCGC	1 cut(s)	178
CviAII	CATG	3 cut(s)	130, 291, 350
CviJI	RGCY	6 cut(s)	154, 185, 212, 280, 328, 355
CviKI_1	RGCY	6 cut(s)	154, 185, 212, 280, 328, 355
DdeI	CTNAG	1 cut(s)	201
DpnI	GATC	4 cut(s)	105, 150, 198, 348
DpnII	GATC	4 cut(s)	103, 148, 196, 346
Eam1104I	CTCTTC	1 cut(s)	228
EarI	CTCTTC	1 cut(s)	228
EciI	GGCGGA	1 cut(s)	393
Eco130I	CCWWGG	1 cut(s)	457
Eco147I	AGGCCT	1 cut(s)	185
Eco24I	GRGCYC	1 cut(s)	357
Eco81I	CCTNAGG	1 cut(s)	201
EcoNI	CCTNNNNNAGG	1 cut(s)	167
EcoT14I	CCWWGG	1 cut(s)	457
EcoT38I	GRGCYC	1 cut(s)	357
ErhI	CCWWGG	1 cut(s)	457
FaeI	CATG	3 cut(s)	133, 294, 353
FaiI	YATR	9 cut(s)	38, 100, 102, 131, 216, 283, 292, 351, 430
FatI	CATG	3 cut(s)	129, 290, 349
FauNDI	CATATG	1 cut(s)	100
FbaI	TGATCA	1 cut(s)	346
FriOI	GRGCYC	1 cut(s)	357
FspBI	CTAG	1 cut(s)	329
FspI	TGCGCA	1 cut(s)	177
GlaI	GCGC	1 cut(s)	177
HaeIII	GGCC	1 cut(s)	185
HhaI	GCGC	1 cut(s)	178
Hin1II	CATG	3 cut(s)	133, 294, 353
Hin6I	GCGC	1 cut(s)	176
HinP1I	GCGC	1 cut(s)	176
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HinfI	GANTC	2 cut(s)	409, 449
Hpy166II	GTNNAC	1 cut(s)	96
Hpy188I	TCNGA	1 cut(s)	57
Hpy188III	TCNNGA	1 cut(s)	200
Hpy8I	GTNNAC	1 cut(s)	96
HpyAV	CCTTC	3 cut(s)	57, 163, 175
HpyCH4V	TGCA	2 cut(s)	257, 265
HpyF10VI	GCNNNNNNNGC	3 cut(s)	182, 224, 341
HpyF3I	CTNAG	1 cut(s)	201
Hsp92II	CATG	3 cut(s)	133, 294, 353
HspAI	GCGC	1 cut(s)	176
Ksp22I	TGATCA	1 cut(s)	346
Kzo9I	GATC	4 cut(s)	103, 148, 196, 346
LpnPI	CCDG	7 cut(s)	66, 120, 179, 199, 213, 300, 459
LweI	GCATC	2 cut(s)	33, 331
MaeI	CTAG	1 cut(s)	329
MalI	GATC	4 cut(s)	105, 150, 198, 348
MboI	GATC	4 cut(s)	103, 148, 196, 346
MboII	GAAGA	2 cut(s)	16, 245
MflI	RGATCY	1 cut(s)	196
MhlI	GDGCHC	1 cut(s)	357
MluCI	AATT	3 cut(s)	30, 65, 266
MlyI	GAGTC	2 cut(s)	403, 443
MnlI	CCTC	4 cut(s)	88, 127, 196, 269
MseI	TTAA	3 cut(s)	378, 438, 463
MslI	CAYNNNNRTG	1 cut(s)	41
Mva1269I	GAATGC	1 cut(s)	259
MwoI	GCNNNNNNNGC	3 cut(s)	182, 224, 341
NdeI	CATATG	1 cut(s)	100
NdeII	GATC	4 cut(s)	103, 148, 196, 346
NlaIII	CATG	3 cut(s)	133, 294, 353
NlaIV	GGNNCC	1 cut(s)	198
NsbI	TGCGCA	1 cut(s)	177
NspI	RCATGY	1 cut(s)	294
PceI	AGGCCT	1 cut(s)	185
PctI	GAATGC	1 cut(s)	259
PflFI	GACNNNGTC	1 cut(s)	452
PleI	GAGTC	2 cut(s)	403, 443
PpsI	GAGTC	2 cut(s)	403, 443
PspN4I	GGNNCC	1 cut(s)	198
PsuI	RGATCY	1 cut(s)	196
PsyI	GACNNNGTC	1 cut(s)	452
RseI	CAYNNNNRTG	1 cut(s)	41
SaqAI	TTAA	3 cut(s)	378, 438, 463
Sau3AI	GATC	4 cut(s)	103, 148, 196, 346
SchI	GAGTC	2 cut(s)	403, 443
SduI	GDGCHC	1 cut(s)	357
SetI	ASST	9 cut(s)	63, 165, 174, 207, 214, 330, 384, 448, 463
SfaNI	GCATC	2 cut(s)	33, 331
SfcI	CTRYAG	1 cut(s)	164
SmiMI	CAYNNNNRTG	1 cut(s)	41
Sse9I	AATT	3 cut(s)	30, 65, 266
SseBI	AGGCCT	1 cut(s)	185
SsiI	CCGC	1 cut(s)	404
SspMI	CTAG	1 cut(s)	329
StuI	AGGCCT	1 cut(s)	185
StyI	CCWWGG	1 cut(s)	457
TasI	AATT	3 cut(s)	30, 65, 266
Tru1I	TTAA	3 cut(s)	378, 438, 463
Tru9I	TTAA	3 cut(s)	378, 438, 463
TspDTI	ATGAA	2 cut(s)	17, 456
TspGWI	ACGGA	1 cut(s)	404
Tth111I	GACNNNGTC	1 cut(s)	452
XagI	CCTNNNNNAGG	1 cut(s)	167
XceI	RCATGY	1 cut(s)	294
XspI	CTAG	1 cut(s)	329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.