Rh5CG239900

Olee1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017264
Physical Location & Seq
Forward (+)
24923536 .. 24925252
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG239900.1

Sequence Viewer

Length: 534 bp
ATGGAAAAGAACTTGGCAATCGTTGCAGTTGTTCTCACCGTGTCTTGCTTGTCATCTTTGGCCTATTGCGACGACGGCAACAGTGACGGCATCAATGTTGAGGGACAAGTGTATTGCGACCCATGCCGTGTTCAGTTCCCAACCAGAGTCAGTAAACCGATAGAAGGCGCCAAAGTAAAATTGGAGTGCAGGAGTCGTGAAAATGAAACTGTCGTGTATACGCTTGAAGGAGAGACCAATGATAAAGGCACCTATAGCCTTCCTGTGCATAAAGACTTTCAGGAGCAGATTTGTGAGGTGACAACAGTGGAAAGCCCGAAAGATGATTGCAATGAAAAATTTGACGATATTGAAAGAGCTAGAATTTTGATCACAAAAAAGAATGGCATCGAATCCAAGACTCGTTATGCCAACCCCCTTGGGTTCATGAAGAAAGAAACTAGTGACGAGTGCAAGGAAGTTCTTAAGGAGTTGTTCCCGGACGAGGACGACATGCCAGAAGAATTTAAGAAAAAGATCGGGGAATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

177

Amino Acids

20.02

Weight (kDa)

4.79

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 33 - 113 1.9e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017264)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18490
rosa_chinensis RchiOBHm_Chr5g0031251
rosa_laevigata RLG00000033294
rosa_multiflora Rmu_co8447941.1_g000001 Rmu_sc0004014.1_g000006
rosa_roxburghii Rroxscaffold_1G00049040
rosa_rugosa Rorug05G0123200
rosa_samantha Rh5AG216700 Rh5BG216100 Rh5CG239900 Rh5DG221300
rosa_wichuraiana Rw0G010920 Rw5G019820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 167, 248
AccI GTMKAC 1 cut(s) 218
AcsI RAATTY 3 cut(s) 338, 363, 503
AcyI GRCGYC 1 cut(s) 168
AfiI CCNNNNNNNGG 2 cut(s) 164, 484
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 2 cut(s) 227, 353
AhlI ACTAGT 1 cut(s) 440
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 227
AoxI GGCC 1 cut(s) 60
ApoI RAATTY 3 cut(s) 338, 363, 503
AspLEI GCGC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 28, 310
BanI GGYRCC 2 cut(s) 167, 248
BceAI ACGGC 3 cut(s) 91, 103, 111
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 2 cut(s) 360, 441
BfmI CTRYAG 1 cut(s) 253
BfoI RGCGCY 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 464
Bme1390I CCNGG 1 cut(s) 479
BmiI GGNNCC 2 cut(s) 169, 250
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 99, 396
BpuMI CCSGG 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 484
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 164, 484
BseMI GCAATG 1 cut(s) 337
BsgI GTGCAG 1 cut(s) 208
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 2 cut(s) 167, 248
BsiSI CCGG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 164, 484
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 62
Bso31I GGTCTC 1 cut(s) 227
Bsp143I GATC 2 cut(s) 369, 516
BspANI GGCC 1 cut(s) 62
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 169, 250
BspT107I GGYRCC 2 cut(s) 167, 248
BspTI CTTAAG 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 2 cut(s) 369, 516
BssNAI GTATAC 1 cut(s) 219
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 219
Bst4CI ACNGT 4 cut(s) 40, 83, 211, 307
BstACI GRCGYC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 372, 519
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 369, 516
BstMWI GCNNNNNNNGC 4 cut(s) 23, 75, 123, 255
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 253
BstZ17I GTATAC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 62
BtsIMutI CAGTG 2 cut(s) 88, 312
CciI TCATGA 1 cut(s) 426
CfoI GCGC 1 cut(s) 170
CviAII CATG 3 cut(s) 123, 427, 493
CviJI RGCY 4 cut(s) 62, 258, 315, 359
CviKI_1 RGCY 4 cut(s) 62, 258, 315, 359
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 371, 518
DpnII GATC 2 cut(s) 369, 516
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 418
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 3 cut(s) 126, 430, 496
FaiI YATR 7 cut(s) 124, 219, 255, 270, 408, 428, 494
FaqI GGGAC 1 cut(s) 117
FatI CATG 3 cut(s) 122, 426, 492
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 218
FspBI CTAG 2 cut(s) 360, 441
GlaI GCGC 1 cut(s) 169
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 62
HapII CCGG 1 cut(s) 479
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 3 cut(s) 126, 430, 496
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 5 cut(s) 147, 193, 392, 400, 524
HpaII CCGG 1 cut(s) 479
HphI GGTGA 2 cut(s) 28, 310
Hpy166II GTNNAC 2 cut(s) 155, 219
Hpy188III TCNNGA 3 cut(s) 197, 281, 427
Hpy8I GTNNAC 2 cut(s) 155, 219
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 3 cut(s) 158, 221, 269
HpyCH4III ACNGT 4 cut(s) 40, 83, 211, 307
HpyCH4V TGCA 5 cut(s) 26, 189, 268, 330, 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 75, 123, 255
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 3 cut(s) 126, 430, 496
HspAI GCGC 1 cut(s) 168
KasI GGCGCC 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 2 cut(s) 369, 516
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 6 cut(s) 157, 175, 266, 276, 492, 510
LweI GCATC 2 cut(s) 99, 396
MaeI CTAG 2 cut(s) 360, 441
MaeIII GTNAC 3 cut(s) 83, 298, 443
MalI GATC 2 cut(s) 371, 518
MboI GATC 2 cut(s) 369, 516
MboII GAAGA 2 cut(s) 442, 512
MluCI AATT 4 cut(s) 179, 338, 363, 503
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 3 cut(s) 156, 202, 394
MnlI CCTC 3 cut(s) 94, 289, 478
MseI TTAA 2 cut(s) 465, 507
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 1 cut(s) 479
MwoI GCNNNNNNNGC 4 cut(s) 23, 75, 123, 255
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 1 cut(s) 479
NdeII GATC 2 cut(s) 369, 516
NlaIII CATG 3 cut(s) 126, 430, 496
NlaIV GGNNCC 2 cut(s) 169, 250
NmuCI GTSAC 3 cut(s) 83, 298, 443
NspI RCATGY 1 cut(s) 496
PagI TCATGA 1 cut(s) 426
PfeI GAWTC 2 cut(s) 392, 524
PfoI TCCNGGA 1 cut(s) 477
PleI GAGTC 3 cut(s) 155, 201, 394
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 3 cut(s) 155, 201, 394
PspN4I GGNNCC 2 cut(s) 169, 250
SaqAI TTAA 2 cut(s) 465, 507
Sau3AI GATC 2 cut(s) 369, 516
SchI GAGTC 3 cut(s) 156, 202, 394
ScrFI CCNGG 1 cut(s) 479
SetI ASST 3 cut(s) 254, 300, 361
SfaNI GCATC 2 cut(s) 99, 396
SfcI CTRYAG 1 cut(s) 253
SfoI GGCGCC 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 4 cut(s) 179, 338, 363, 503
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 2 cut(s) 360, 441
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 4 cut(s) 40, 83, 211, 307
TaqI TCGA 1 cut(s) 390
TasI AATT 4 cut(s) 179, 338, 363, 503
TfiI GAWTC 2 cut(s) 392, 524
Tru1I TTAA 2 cut(s) 465, 507
Tru9I TTAA 2 cut(s) 465, 507
TscAI CASTG 2 cut(s) 88, 312
TseFI GTSAC 3 cut(s) 83, 298, 443
Tsp45I GTSAC 3 cut(s) 83, 298, 443
TspDTI ATGAA 4 cut(s) 219, 348, 415, 443
TspRI CASTG 2 cut(s) 88, 312
Vha464I CTTAAG 1 cut(s) 464
XapI RAATTY 3 cut(s) 338, 363, 503
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 218
XspI CTAG 2 cut(s) 360, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.