Rh5DG031200

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0029389
Physical Location & Seq
Reverse (-)
2211031 .. 2214910
3880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG031200.1

Sequence Viewer

Length: 594 bp
ATGACGAGAATGCCACCGCCTTCGCTGCTGACGTTGGGGCCGATGGAGGCCGTGGCGTTCGTGATGGCCTGGTGGGACGATATCAACGAGTCGACTAAGTGGCAGGACGGCATCTTCTACTCTCTCTGCGCCGCCTACGCCCTCGTCTCCTCCGTCGCTCTGTTTCAACTAATAAGGATTGAATTGAGAGTGCCGGAGTACGGTTGGACGACGCAGAAGGTGTTTCATCTTATGAATTTCATTGTCAATGGAGTGCGCGCAATTGTCTTTGGATGTCACAAGCAAGTCTTTCTCTTGCAACCAACGGTGTTGATATGGGTCTTATTGGATCTTCCTGGACTGCTATTCTTCTCAACGTACACACTTCTTGTCCTATTTTGGGCAGAGATATATCACCAGGCAAGAAGTTTACCGACAGATAAGCTCAGGATTGTTTATATTTCAGTCAATGCAGGAATGTACTTCGTACAGATTTGCCTCTGGATATACCTATGGTTTGATGACAACAGCGTTGTGCAGTTCATTGGAAAGATATTTATTGCAGTTGTGTCATTTTTGGCCGCACTAGGTTTCTTAGTCTATGGAGGAAGGTGA

Protein Analysis

197

Amino Acids

22.63

Weight (kDa)

6.81

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 197 3.9e-81 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029389)

Species Orthologous Gene IDs
pyrus_communis pycom10g26980
rosa_samantha Rh5DG031200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 92
AccII CGCG 1 cut(s) 258
AciI CCGC 3 cut(s) 17, 132, 561
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 4 cut(s) 200, 359, 461, 468
AfiI CCNNNNNNNGG 2 cut(s) 200, 379
AgsI TTSAA 2 cut(s) 167, 182
AjnI CCWGG 3 cut(s) 68, 334, 396
AluBI AGCT 1 cut(s) 424
AluI AGCT 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 1 cut(s) 336
AoxI GGCC 4 cut(s) 38, 48, 66, 558
ApeKI GCWGC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 3 cut(s) 131, 258, 260
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 386
BbvI GCAGC 1 cut(s) 12
BccI CCATC 2 cut(s) 37, 58
BceAI ACGGC 2 cut(s) 35, 124
BciT130I CCWGG 3 cut(s) 70, 336, 398
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 1 cut(s) 566
BisI GCNGC 3 cut(s) 26, 132, 561
BlsI GCNGC 3 cut(s) 27, 133, 562
Bme1390I CCNGG 3 cut(s) 70, 336, 398
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 3 cut(s) 70, 336, 398
BmsI GCATC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 379
BseBI CCWGG 3 cut(s) 70, 336, 398
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 278
BseLI CCNNNNNNNGG 2 cut(s) 200, 379
BseMII CTCAG 1 cut(s) 439
BsePI GCGCGC 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 4 cut(s) 40, 50, 68, 560
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 200, 379
BsmAI GTCTC 1 cut(s) 151
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 4 cut(s) 40, 50, 68, 560
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 3 cut(s) 17, 132, 561
BspANI GGCC 4 cut(s) 40, 50, 68, 560
BspCNI CTCAG 1 cut(s) 438
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 51
BssHII GCGCGC 1 cut(s) 256
BssMI GATC 1 cut(s) 328
Bst2UI CCWGG 3 cut(s) 70, 336, 398
Bst4CI ACNGT 2 cut(s) 203, 307
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 96, 425, 574
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 3 cut(s) 131, 258, 260
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 2 cut(s) 25, 137
BstNI CCWGG 3 cut(s) 70, 336, 398
BstSCI CCNGG 3 cut(s) 68, 334, 396
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 4 cut(s) 40, 50, 68, 560
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 3 cut(s) 131, 258, 260
Cfr13I GGNCC 1 cut(s) 38
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 199, 358, 460, 467
CviJI RGCY 5 cut(s) 40, 50, 68, 424, 560
CviKI_1 RGCY 5 cut(s) 40, 50, 68, 424, 560
CviQI GTAC 4 cut(s) 199, 358, 460, 467
DdeI CTNAG 3 cut(s) 96, 425, 574
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 558
Eco32I GATATC 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 68, 334, 396
EcoRV GATATC 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 151
FaiI YATR 7 cut(s) 233, 316, 391, 438, 487, 493, 582
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 89
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 3 cut(s) 26, 132, 561
FokI GGATG 1 cut(s) 285
Fsp4HI GCNGC 3 cut(s) 26, 132, 561
FspBI CTAG 1 cut(s) 566
GlaI GCGC 3 cut(s) 130, 257, 259
GluI GCNGC 3 cut(s) 26, 132, 561
HaeIII GGCC 4 cut(s) 40, 50, 68, 560
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 220
HhaI GCGC 3 cut(s) 131, 258, 260
Hin6I GCGC 3 cut(s) 129, 256, 258
HinP1I GCGC 3 cut(s) 129, 256, 258
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 1 cut(s) 89
HpaII CCGG 1 cut(s) 194
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 3 cut(s) 93, 360, 410
Hpy188III TCNNGA 3 cut(s) 61, 427, 481
Hpy8I GTNNAC 3 cut(s) 93, 360, 410
Hpy99I CGWCG 2 cut(s) 158, 214
HpyAV CCTTC 3 cut(s) 30, 211, 582
HpyCH4III ACNGT 2 cut(s) 203, 307
HpyCH4IV ACGT 2 cut(s) 32, 356
HpyCH4V TGCA 4 cut(s) 298, 452, 517, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 137
HpyF3I CTNAG 3 cut(s) 96, 425, 574
HpySE526I ACGT 2 cut(s) 32, 356
HspAI GCGC 3 cut(s) 129, 256, 258
Kzo9I GATC 1 cut(s) 328
Lsp1109I GCAGC 1 cut(s) 12
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 32, 356
MaeIII GTNAC 1 cut(s) 275
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 3 cut(s) 106, 323, 340
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 1 cut(s) 328
MluCI AATT 3 cut(s) 182, 235, 261
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 5 cut(s) 40, 152, 160, 488, 578
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 70, 336, 398
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 3 cut(s) 70, 336, 398
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 25, 137
NdeII GATC 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 275
PauI GCGCGC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 4 cut(s) 29, 38, 84, 150
PctI GAATGC 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 3 cut(s) 27, 133, 562
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 3 cut(s) 68, 334, 396
PspGI CCWGG 3 cut(s) 68, 334, 396
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 38
PsuI RGATCY 1 cut(s) 328
PteI GCGCGC 1 cut(s) 256
RsaI GTAC 4 cut(s) 200, 359, 461, 468
RsaNI GTAC 4 cut(s) 199, 358, 460, 467
SalI GTCGAC 1 cut(s) 91
SatI GCNGC 3 cut(s) 26, 132, 561
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 70, 336, 398
SetI ASST 7 cut(s) 35, 222, 359, 426, 492, 571, 593
SfaNI GCATC 1 cut(s) 120
Sse9I AATT 3 cut(s) 182, 235, 261
SsiI CCGC 3 cut(s) 17, 132, 561
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 3 cut(s) 68, 334, 396
TaaI ACNGT 2 cut(s) 203, 307
TaiI ACGT 2 cut(s) 35, 359
TaqI TCGA 1 cut(s) 92
TasI AATT 3 cut(s) 182, 235, 261
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 134, 563
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 4 cut(s) 215, 229, 248, 511
TspGWI ACGGA 1 cut(s) 142
XapI RAATTY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 92
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.