Rh5DG521400

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0022712
Physical Location & Seq
Reverse (-)
80925488 .. 80926225
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG521400.1

Sequence Viewer

Length: 558 bp
ATGGAGCGGCAGAAGGAGCAAAAACAAGGACCGCAAATGGGGATTGGTTTGGAGACCTTACCCAGCTGCGTATTGATCACCGCTATCCTCCCCAAGCTGGAACTGGAAACCCTGTGCTCTCTCGCTTGCGTCAGCAGAGCAATGAACTCCTCAGTCTCTCAAGCCCTTCCTTCACTCTCCTCCCTCAATCTCTCCGCGTTTTCTGCGAATGCCCAGATTTTGAGTAGCATTGTTGGTGGGTGCAGAGGCCTTAATAGCCTCACCCTCAATTGCCTGCGCCTAGACGACTCGTCGTTGGCGGTTTTTCTGGGTAAGCATCTCCAAGAACTGAACTTGCTGTGCTGCTCCTTGATGTCGTATCAAGTCCTTGCTTCCATTGGAGAGGCTTGCCCCAATCTCAGGGTGCTTGTGCTAGAATTGGTAGACCAGCAATCACCTGAAGCTTTTAAGATCAACCTGGACAGAATGCTTAATGGTTGTTTATGCTTGGAGGTATCAATTTTTAGCTCAAATTATAATTCTGATTCTGGTGTTTTAGGGTCTGATTATCATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

185

Amino Acids

19.9

Weight (kDa)

4.88

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 62 - 165 3.8e-25 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022712)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0493531
rosa_multiflora Rmu_co8163004.1_g000001
rosa_samantha Rh4DG094700 Rh5DG521400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AccBSI CCGCTC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 197
AciI CCGC 5 cut(s) 7, 32, 81, 195, 299
AcuI CTGAAG 1 cut(s) 459
AfiI CCNNNNNNNGG 3 cut(s) 38, 97, 399
AhdI GACNNNNNGTC 1 cut(s) 289
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 4 cut(s) 66, 97, 443, 507
AluI AGCT 4 cut(s) 66, 97, 443, 507
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 2 cut(s) 47, 160
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 66, 342
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 29
AsuHPI GGTGA 3 cut(s) 70, 253, 426
AvaII GGWCC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 53, 329
BciT130I CCWGG 1 cut(s) 458
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 47, 160
BfaI CTAG 2 cut(s) 281, 413
BisI GCNGC 3 cut(s) 8, 67, 343
BlsI GCNGC 3 cut(s) 9, 68, 344
Bme1390I CCNGG 1 cut(s) 458
Bme18I GGWCC 1 cut(s) 29
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 1 cut(s) 325
BpuEI CTTGAG 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 47
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 97, 399
Bse1I ACTGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 147
BseBI CCWGG 1 cut(s) 458
BseLI CCNNNNNNNGG 3 cut(s) 38, 97, 399
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 165, 412
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 2 cut(s) 139, 169
BseXI GCAGC 2 cut(s) 53, 329
BseYI CCCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 38, 97, 399
BsmAI GTCTC 2 cut(s) 47, 160
BsmI GAATGC 2 cut(s) 214, 471
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 2 cut(s) 75, 450
BspACI CCGC 5 cut(s) 7, 32, 81, 195, 299
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 2 cut(s) 164, 411
BspFNI CGCG 1 cut(s) 197
BspHI TCATGA 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 147
BsrI ACTGG 1 cut(s) 108
BssMI GATC 2 cut(s) 75, 450
Bst2UI CCWGG 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 127, 275, 388
BstDEI CTNAG 2 cut(s) 151, 398
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 2 cut(s) 78, 453
BstMAI GTCTC 2 cut(s) 47, 160
BstMBI GATC 2 cut(s) 75, 450
BstMWI GCNNNNNNNGC 3 cut(s) 16, 203, 255
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 456
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 53, 329
BsuRI GGCC 1 cut(s) 249
Cac8I GCNNGC 3 cut(s) 127, 275, 388
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 29
CseI GACGC 1 cut(s) 118
CviAII CATG 1 cut(s) 551
CviJI RGCY 8 cut(s) 66, 97, 164, 249, 258, 386, 443, 507
CviKI_1 RGCY 8 cut(s) 66, 97, 164, 249, 258, 386, 443, 507
DdeI CTNAG 2 cut(s) 151, 398
DpnI GATC 2 cut(s) 77, 452
DpnII GATC 2 cut(s) 75, 450
DriI GACNNNNNGTC 1 cut(s) 289
Eam1105I GACNNNNNGTC 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 456
FaeI CATG 1 cut(s) 554
FaiI YATR 3 cut(s) 484, 516, 552
FatI CATG 1 cut(s) 550
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 8, 67, 343
Fsp4HI GCNGC 3 cut(s) 8, 67, 343
FspBI CTAG 2 cut(s) 281, 413
GlaI GCGC 1 cut(s) 278
GluI GCNGC 3 cut(s) 8, 67, 343
GsaI CCCAGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 249
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 279
Hin1II CATG 1 cut(s) 554
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 2 cut(s) 287, 524
HphI GGTGA 3 cut(s) 70, 253, 426
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 2 cut(s) 523, 544
Hpy188III TCNNGA 1 cut(s) 551
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 7, 176, 180
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 203, 255
HpyF3I CTNAG 2 cut(s) 151, 398
Hsp92II CATG 1 cut(s) 554
HspAI GCGC 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 2 cut(s) 75, 450
LmnI GCTCC 3 cut(s) 4, 16, 350
Lsp1109I GCAGC 2 cut(s) 53, 329
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 281, 413
MalI GATC 2 cut(s) 77, 452
MbiI CCGCTC 1 cut(s) 7
MboI GATC 2 cut(s) 75, 450
MfeI CAATTG 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 5 cut(s) 268, 416, 498, 511, 517
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 9 cut(s) 98, 160, 190, 194, 239, 269, 275, 376, 484
MseI TTAA 3 cut(s) 252, 447, 471
MspA1I CMGCKG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 458
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 2 cut(s) 214, 471
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 16, 203, 255
NdeII GATC 2 cut(s) 75, 450
NlaIII CATG 1 cut(s) 554
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 1 cut(s) 249
PctI GAATGC 2 cut(s) 214, 471
PfeI GAWTC 1 cut(s) 524
PkrI GCNGC 3 cut(s) 9, 68, 344
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PsiI TTATAA 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 456
PspPI GGNCC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 66
SaqAI TTAA 3 cut(s) 252, 447, 471
SatI GCNGC 3 cut(s) 8, 67, 343
Sau3AI GATC 2 cut(s) 75, 450
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 1 cut(s) 119
SetI ASST 8 cut(s) 59, 68, 99, 439, 445, 459, 495, 509
SfaNI GCATC 1 cut(s) 325
SinI GGWCC 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 5 cut(s) 268, 416, 498, 511, 517
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 5 cut(s) 7, 32, 81, 195, 299
SspMI CTAG 2 cut(s) 281, 413
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 456
TasI AATT 5 cut(s) 268, 416, 498, 511, 517
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 3 cut(s) 252, 447, 471
Tru9I TTAA 3 cut(s) 252, 447, 471
TseI GCWGC 2 cut(s) 66, 342
TspDTI ATGAA 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 2 cut(s) 281, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.