Rh6AG257200

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0025057
Physical Location & Seq
Reverse (-)
44745943 .. 44748145
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG257200.1

Sequence Viewer

Length: 708 bp
ATGGCTGCCCTTCCCCTACCCCCTATTTATTCAAAAGCCCTAATTAAAGCAAGTGAACTCAACTGCCTGGAAGAGATGTTGATAATTGTTGCAATGCTCTCAGTAGAATCTACGTTTTTCGCTCCCAGTGAGAAGTTAGAAGAGGCGAGAACTGCAAGGAAACGCTTTGAAAGTCCAGAGGGAGATCATCTCACTTTGATTAATCTATATCTAGCATCTGATCAGTTTCTCAAGAGTAGATCAGGTCTTAGCACAGGGAACCATGAACAAAATTTCCAGAAATGGTGCAAGGAATATTTCATTGATAGTTGCTCCCTGAGAAGTGCTCGTGAGATTCATAGCCAAATTCGACAAAGTGTTGAACAAATGGGCCTTTGTGTTGCATCTTGCAGGGACAGCATGTTTCAGTTTCGTAGATGTCTTGCTGCTTCTTTCTTCCTCAAAGTAGCTTTAAAGGAATCTGATGGAGAATACAGGGTTTTAACATCTGATCTGGCAAATGAACTGGTTGTTCGGATTCACCCATCTTCTGTGATGTCCAAGAAAAAACCAGAGTGCATAGTTTTTAATGAACTAATTGAAACTAAAGACAAAAGGTTTATCTGCAACACGACTAGAATCAACTATCCATGGTTAAGTGAGCTTGCTCCTCCACGACTAAAAAAGGTATGGGAAAGACACAAGCTACCTTGGATCTCTAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

235

Amino Acids

27.07

Weight (kDa)

8.7

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 7 - 66 2.7e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 138 - 217 5.9e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025057)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0281851
rosa_samantha Rh6AG257200 Rh6DG251500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 701
AcsI RAATTY 2 cut(s) 271, 345
AgsI TTSAA 4 cut(s) 33, 170, 362, 581
AjnI CCWGG 1 cut(s) 66
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 449, 643, 685
AluI AGCT 3 cut(s) 449, 643, 685
Alw21I GWGCWC 1 cut(s) 328
AlwI GGATC 1 cut(s) 701
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 2 cut(s) 5, 425
ApoI RAATTY 2 cut(s) 271, 345
AseI ATTAAT 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 512
BauI CACGAG 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 458, 532
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 1 cut(s) 220
BfaI CTAG 2 cut(s) 212, 615
BisI GCNGC 2 cut(s) 6, 426
BlsI GCNGC 2 cut(s) 7, 427
Bme1390I CCNGG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 68
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 224, 392
BmuI ACTGGG 1 cut(s) 120
BplI GAGNNNNNCTC 4 cut(s) 174, 206, 310, 342
BpuEI CTTGAG 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 629, 689
Bse1I ACTGG 2 cut(s) 126, 510
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 629, 689
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 2 cut(s) 114, 308
BseNI ACTGG 2 cut(s) 126, 510
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 328
BslFI GGGAC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 5 cut(s) 184, 220, 239, 490, 693
Bsp19I CCATGG 1 cut(s) 629
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 2 cut(s) 113, 309
BspLI GGNNCC 1 cut(s) 260
BspPI GGATC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 2 cut(s) 126, 510
BssECI CCNNGG 2 cut(s) 629, 689
BssMI GATC 5 cut(s) 184, 220, 239, 490, 693
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 2 cut(s) 629, 689
Bst2BI CACGAG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 68
Bst6I CTCTTC 2 cut(s) 66, 135
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 3 cut(s) 100, 248, 317
BstDSI CCRYGG 1 cut(s) 629
BstKTI GATC 5 cut(s) 187, 223, 242, 493, 696
BstMBI GATC 5 cut(s) 184, 220, 239, 490, 693
BstMWI GCNNNNNNNGC 2 cut(s) 152, 396
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 693
BstYI RGATCY 1 cut(s) 693
BsuRI GGCC 1 cut(s) 372
BtgI CCRYGG 1 cut(s) 629
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 645
Cfr13I GGNCC 1 cut(s) 370
CviAII CATG 3 cut(s) 263, 400, 630
CviJI RGCY 7 cut(s) 5, 38, 342, 372, 449, 643, 685
CviKI_1 RGCY 7 cut(s) 5, 38, 342, 372, 449, 643, 685
DdeI CTNAG 3 cut(s) 100, 248, 317
DpnI GATC 5 cut(s) 186, 222, 241, 492, 695
DpnII GATC 5 cut(s) 184, 220, 239, 490, 693
DraI TTTAAA 1 cut(s) 453
Eam1104I CTCTTC 2 cut(s) 66, 135
EarI CTCTTC 2 cut(s) 66, 135
Eco130I CCWWGG 2 cut(s) 629, 689
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 2 cut(s) 629, 689
ErhI CCWWGG 2 cut(s) 629, 689
FaeI CATG 3 cut(s) 266, 403, 633
FaiI YATR 7 cut(s) 208, 264, 339, 401, 560, 631, 670
FaqI GGGAC 1 cut(s) 407
FatI CATG 3 cut(s) 262, 399, 629
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 6, 426
Fsp4HI GCNGC 2 cut(s) 6, 426
FspBI CTAG 2 cut(s) 212, 615
GluI GCNGC 2 cut(s) 6, 426
HaeIII GGCC 1 cut(s) 372
Hin1II CATG 3 cut(s) 266, 403, 633
HinfI GANTC 5 cut(s) 107, 334, 458, 517, 618
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 4 cut(s) 220, 463, 490, 516
Hpy188III TCNNGA 4 cut(s) 176, 232, 277, 329
Hpy8I GTNNAC 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 20
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 7 cut(s) 92, 155, 288, 383, 390, 558, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 396
HpyF3I CTNAG 3 cut(s) 100, 248, 317
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 3 cut(s) 266, 403, 633
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 5 cut(s) 184, 220, 239, 490, 693
LmnI GCTCC 3 cut(s) 127, 317, 652
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 224, 392
MaeI CTAG 2 cut(s) 212, 615
MaeII ACGT 1 cut(s) 113
MalI GATC 5 cut(s) 186, 222, 241, 492, 695
MboI GATC 5 cut(s) 184, 220, 239, 490, 693
MboII GAAGA 4 cut(s) 83, 152, 427, 519
MflI RGATCY 1 cut(s) 693
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 6 cut(s) 42, 84, 271, 345, 576, 700
MnlI CCTC 4 cut(s) 136, 172, 449, 660
MseI TTAA 6 cut(s) 45, 201, 452, 482, 567, 635
MspR9I CCNGG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 152, 396
NcoI CCATGG 1 cut(s) 629
NdeII GATC 5 cut(s) 184, 220, 239, 490, 693
NlaIII CATG 3 cut(s) 266, 403, 633
NlaIV GGNNCC 1 cut(s) 260
NspI RCATGY 1 cut(s) 403
PfeI GAWTC 5 cut(s) 107, 334, 458, 517, 618
PkrI GCNGC 2 cut(s) 7, 427
PshBI ATTAAT 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 370
PsuI RGATCY 1 cut(s) 693
SaqAI TTAA 6 cut(s) 45, 201, 452, 482, 567, 635
SatI GCNGC 2 cut(s) 6, 426
Sau3AI GATC 5 cut(s) 184, 220, 239, 490, 693
Sau96I GGNCC 1 cut(s) 370
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 1 cut(s) 328
SetI ASST 8 cut(s) 116, 247, 451, 599, 645, 669, 687, 691
SfaNI GCATC 2 cut(s) 224, 392
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 6 cut(s) 42, 84, 271, 345, 576, 700
SspI AATATT 1 cut(s) 296
SspMI CTAG 2 cut(s) 212, 615
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 2 cut(s) 629, 689
TaiI ACGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 349
TasI AATT 6 cut(s) 42, 84, 271, 345, 576, 700
TfiI GAWTC 5 cut(s) 107, 334, 458, 517, 618
Tru1I TTAA 6 cut(s) 45, 201, 452, 482, 567, 635
Tru9I TTAA 6 cut(s) 45, 201, 452, 482, 567, 635
TscAI CASTG 1 cut(s) 133
TseI GCWGC 2 cut(s) 5, 425
TspDTI ATGAA 5 cut(s) 279, 289, 326, 516, 585
TspRI CASTG 1 cut(s) 133
VspI ATTAAT 1 cut(s) 201
XapI RAATTY 2 cut(s) 271, 345
XceI RCATGY 1 cut(s) 403
XspI CTAG 2 cut(s) 212, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.