Rh6CG436300

GTP1/OBG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012584
Physical Location & Seq
Forward (+)
61583486 .. 61587615
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG436300.1

Sequence Viewer

Length: 1044 bp
ATGGCGTCTCTCTTCTCCACACCAACTTCAACTTCAACTTCAACTTCGTATCTCTTCCGTCAAAAATCCTCCTTATACGGCACCGTTTTCCCTCTCAGGTGTGACCGGAAGCCCGGCAATTTGAGAAGCACCGGTTACTTTGAAATTAGCTGCCGACTTAGCACCAGAGCCAAAGAGTCGTCGCCGTCTCCGGCTTTAAACCTGAACAAGGAGCCTCACAAGTACTTTGACCAGGTCGTCATCACGGTCCGCTCCGGCGACGGCGGCCATGGCGCGGTCCTCAACATGCCGAACCAGAAAGCTAATAAGCCGCCGCAGGGGAAGGTTGAGAAGGAGAAGGCCAGGAGGAGAGGATTGTTGAAGAGGGACTTCGGCGGGTCCCTCGTTCTTCCCACAGGTGGCCATGGAGGTGATGTGGTGATATATGCTGATGAGGAGGAAGATACATTGCTGGAGTTTCACAAGAAAGGGAGGTACAACGCGAAGCGCGGCGGAAATGTGGCTGCTATGGGGGTTTTGACCTCCCAATTGAGAGATGGAGTTGCTGCCCCAACTCTGCGCATTCCTGTGCCTGTAGGTACTGTTGTGAAGCGGAAACGAGGGACGCTGTTGCGTGATCTAGCCCAGCCAGGTGATGAAATCCTTGTAGCGAGGGGTGGACAAGGAGGGATTAGCTTGGTAGAAATGCCAGAGCGTAGCAAGAAAAAGTTGATGGCTTTGACCACTAATGTGATGAGAGATGATAGTGATAAGGTTTTATCACTTGGTCAACCTGGAGAGGAGGTTACTTTGGAGTTGATTCTACGAGTTGTTGCTGATGTTGGTTTGGTTGGCCTCCCAAATGCTGGAAAGTCAACCCTTTTGGCAGCTACTACCCTTGCAAAACCTGATATTGCTGATTATCCTTTCACAACCTTAATGCCAAACCTAGGACGTCTTAATGGTGACCCAAGTTTAGGGGCTCAGATGTATACTTCTGAAGCAACATTGGCAGATTTACCTGGTCTTATTGAAGGTGCTCATCTTGGGAAGGTAATGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP1_OBG MMR_HSR1

Protein Analysis

347

Amino Acids

37.16

Weight (kDa)

9.57

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP1_OBG PF01018 132 - 235 5.6e-18 GTP1/OBG
MMR_HSR1 PF01926 273 - 344 2.8e-13 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AatII GACGTC 1 cut(s) 937
Acc16I TGCGCA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 971
AccII CGCG 3 cut(s) 275, 482, 489
AciI CCGC 9 cut(s) 250, 264, 275, 311, 314, 375, 489, 492, 592
AcoI YGGCCR 2 cut(s) 265, 400
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 2 cut(s) 5, 934
AfaI GTAC 3 cut(s) 224, 476, 580
AfiI CCNNNNNNNGG 7 cut(s) 208, 274, 317, 398, 845, 929, 956
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 6 cut(s) 30, 36, 42, 143, 361, 1013
AjnI CCWGG 5 cut(s) 231, 341, 628, 772, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
AleI CACNNNNGTG 1 cut(s) 728
AluBI AGCT 4 cut(s) 150, 302, 675, 869
AluI AGCT 4 cut(s) 150, 302, 675, 869
Alw21I GWGCWC 1 cut(s) 1021
Alw26I GTCTC 2 cut(s) 12, 192
AoxI GGCC 4 cut(s) 265, 339, 400, 832
ApeKI GCWGC 4 cut(s) 150, 503, 545, 866
AsiGI ACCGGT 1 cut(s) 131
AspA2I CCTAGG 1 cut(s) 928
AspLEI GCGC 3 cut(s) 275, 489, 561
AspS9I GGNCC 3 cut(s) 247, 277, 378
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 4 cut(s) 422, 430, 644, 956
AvaII GGWCC 3 cut(s) 247, 277, 378
AvrII CCTAGG 1 cut(s) 928
BaeI ACNNNNGTAYC 2 cut(s) 570, 603
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 964
Bbv12I GWGCWC 1 cut(s) 1021
BbvI GCAGC 4 cut(s) 137, 490, 532, 878
BccI CCATC 2 cut(s) 530, 706
BceAI ACGGC 3 cut(s) 94, 169, 277
BciT130I CCWGG 5 cut(s) 233, 343, 630, 774, 1002
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 12, 192
BfaI CTAG 3 cut(s) 620, 929, 1042
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 8 cut(s) 151, 265, 311, 314, 490, 504, 546, 867
BlnI CCTAGG 1 cut(s) 928
BlsI GCNGC 8 cut(s) 152, 266, 312, 315, 491, 505, 547, 868
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 6 cut(s) 114, 233, 343, 630, 774, 1002
Bme18I GGWCC 3 cut(s) 247, 277, 378
BmgT120I GGNCC 3 cut(s) 247, 277, 378
BmiI GGNNCC 4 cut(s) 82, 213, 379, 380
BmrFI CCNGG 6 cut(s) 114, 233, 343, 630, 774, 1002
BpmI CTGGAG 2 cut(s) 473, 795
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 2 cut(s) 5, 934
BsaJI CCNNGG 3 cut(s) 268, 403, 928
BsaWI WCCGGW 2 cut(s) 105, 131
BsaXI ACNNNNNCTCC 4 cut(s) 399, 429, 768, 798
Bsc4I CCNNNNNNNGG 7 cut(s) 208, 274, 317, 398, 845, 929, 956
Bse118I RCCGGY 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 446
BseBI CCWGG 5 cut(s) 233, 343, 630, 774, 1002
BseDI CCNNGG 3 cut(s) 268, 403, 928
BseLI CCNNNNNNNGG 7 cut(s) 208, 274, 317, 398, 845, 929, 956
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 2 cut(s) 109, 977
BseRI GAGGAG 3 cut(s) 361, 449, 794
BseXI GCAGC 4 cut(s) 137, 490, 532, 878
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 3 cut(s) 275, 482, 489
BshFI GGCC 4 cut(s) 267, 341, 402, 834
BshNI GGYRCC 1 cut(s) 80
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 1021
BsiSI CCGG 5 cut(s) 106, 114, 132, 191, 255
BslFI GGGAC 3 cut(s) 364, 380, 616
BslI CCNNNNNNNGG 7 cut(s) 208, 274, 317, 398, 845, 929, 956
BsmAI GTCTC 2 cut(s) 12, 192
BsmBI CGTCTC 2 cut(s) 12, 192
BsmFI GGGAC 3 cut(s) 364, 380, 616
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 4 cut(s) 267, 341, 402, 834
Bsp1286I GDGCHC 2 cut(s) 964, 1021
Bsp143I GATC 1 cut(s) 616
Bsp19I CCATGG 2 cut(s) 268, 403
BspACI CCGC 9 cut(s) 250, 264, 275, 311, 314, 375, 489, 492, 592
BspANI GGCC 4 cut(s) 267, 341, 402, 834
BspCNI CTCAG 2 cut(s) 108, 976
BspFNI CGCG 3 cut(s) 275, 482, 489
BspLI GGNNCC 4 cut(s) 82, 213, 379, 380
BspT107I GGYRCC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 268, 403, 928
BssMI GATC 1 cut(s) 616
BssNAI GTATAC 1 cut(s) 972
BssNI GRCGYC 2 cut(s) 5, 934
BssT1I CCWWGG 3 cut(s) 268, 403, 928
Bst1107I GTATAC 1 cut(s) 972
Bst2UI CCWGG 5 cut(s) 233, 343, 630, 774, 1002
Bst4CI ACNGT 3 cut(s) 85, 247, 583
Bst6I CTCTTC 3 cut(s) 17, 59, 356
BstACI GRCGYC 2 cut(s) 5, 934
BstDEI CTNAG 3 cut(s) 95, 158, 963
BstDSI CCRYGG 2 cut(s) 268, 403
BstEII GGTNACC 1 cut(s) 944
BstENI CCTNNNNNAGG 1 cut(s) 206
BstFNI CGCG 3 cut(s) 275, 482, 489
BstHHI GCGC 3 cut(s) 275, 489, 561
BstKTI GATC 1 cut(s) 619
BstMAI GTCTC 2 cut(s) 12, 192
BstMBI GATC 1 cut(s) 616
BstMWI GCNNNNNNNGC 4 cut(s) 159, 264, 270, 989
BstNI CCWGG 5 cut(s) 233, 343, 630, 774, 1002
BstNSI RCATGY 1 cut(s) 289
BstPI GGTNACC 1 cut(s) 944
BstSCI CCNGG 6 cut(s) 112, 231, 341, 628, 772, 1000
BstSFI CTRYAG 1 cut(s) 573
BstUI CGCG 3 cut(s) 275, 482, 489
BstV1I GCAGC 4 cut(s) 137, 490, 532, 878
BstZ17I GTATAC 1 cut(s) 972
BsuRI GGCC 4 cut(s) 267, 341, 402, 834
BtgI CCRYGG 2 cut(s) 268, 403
CfoI GCGC 3 cut(s) 275, 489, 561
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 247, 277, 378
CpoI CGGWCCG 1 cut(s) 247
CseI GACGC 1 cut(s) 613
CsiI ACCWGGT 2 cut(s) 231, 1000
Csp6I GTAC 3 cut(s) 223, 475, 579
CspAI ACCGGT 1 cut(s) 131
CspI CGGWCCG 1 cut(s) 247
CviAII CATG 3 cut(s) 269, 286, 404
CviQI GTAC 3 cut(s) 223, 475, 579
DdeI CTNAG 3 cut(s) 95, 158, 963
DpnI GATC 1 cut(s) 618
DpnII GATC 1 cut(s) 616
DraI TTTAAA 1 cut(s) 198
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 2 cut(s) 265, 400
Eam1104I CTCTTC 3 cut(s) 17, 59, 356
EarI CTCTTC 3 cut(s) 17, 59, 356
EciI GGCGGA 1 cut(s) 507
Eco130I CCWWGG 3 cut(s) 268, 403, 928
Eco24I GRGCYC 1 cut(s) 964
Eco47I GGWCC 3 cut(s) 247, 277, 378
Eco57I CTGAAG 1 cut(s) 999
Eco91I GGTNACC 1 cut(s) 944
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 378
EcoO65I GGTNACC 1 cut(s) 944
EcoRII CCWGG 5 cut(s) 231, 341, 628, 772, 1000
EcoT14I CCWWGG 3 cut(s) 268, 403, 928
EcoT38I GRGCYC 1 cut(s) 964
ErhI CCWWGG 3 cut(s) 268, 403, 928
Esp3I CGTCTC 2 cut(s) 12, 192
FaeI CATG 3 cut(s) 272, 289, 407
FaiI YATR 8 cut(s) 76, 270, 287, 405, 424, 426, 509, 972
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 3 cut(s) 364, 380, 616
FatI CATG 3 cut(s) 268, 285, 403
FauI CCCGC 1 cut(s) 368
FblI GTMKAC 1 cut(s) 971
Fnu4HI GCNGC 8 cut(s) 151, 265, 311, 314, 490, 504, 546, 867
FriOI GRGCYC 1 cut(s) 964
Fsp4HI GCNGC 8 cut(s) 151, 265, 311, 314, 490, 504, 546, 867
FspBI CTAG 3 cut(s) 620, 929, 1042
FspI TGCGCA 1 cut(s) 560
GlaI GCGC 3 cut(s) 274, 488, 560
GluI GCNGC 8 cut(s) 151, 265, 311, 314, 490, 504, 546, 867
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 2 cut(s) 473, 795
HaeIII GGCC 4 cut(s) 267, 341, 402, 834
HapII CCGG 5 cut(s) 106, 114, 132, 191, 255
HgaI GACGC 1 cut(s) 613
HhaI GCGC 3 cut(s) 275, 489, 561
Hin1I GRCGYC 2 cut(s) 5, 934
Hin1II CATG 3 cut(s) 272, 289, 407
Hin6I GCGC 3 cut(s) 273, 487, 559
HinP1I GCGC 3 cut(s) 273, 487, 559
HincII GTYRAC 2 cut(s) 770, 855
HindII GTYRAC 2 cut(s) 770, 855
HinfI GANTC 2 cut(s) 176, 799
HpaII CCGG 5 cut(s) 106, 114, 132, 191, 255
HphI GGTGA 4 cut(s) 422, 430, 644, 956
Hpy166II GTNNAC 4 cut(s) 659, 770, 855, 972
Hpy188I TCNGA 2 cut(s) 966, 979
Hpy8I GTNNAC 4 cut(s) 659, 770, 855, 972
Hpy99I CGWCG 2 cut(s) 184, 263
HpyAV CCTTC 5 cut(s) 316, 325, 331, 1007, 1024
HpyCH4III ACNGT 3 cut(s) 85, 247, 583
HpyCH4IV ACGT 1 cut(s) 934
HpyCH4V TGCA 1 cut(s) 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 264, 270, 989
HpyF3I CTNAG 3 cut(s) 95, 158, 963
HpySE526I ACGT 1 cut(s) 934
Hsp92I GRCGYC 2 cut(s) 5, 934
Hsp92II CATG 3 cut(s) 272, 289, 407
HspAI GCGC 3 cut(s) 273, 487, 559
KflI GGGWCCC 1 cut(s) 378
Kzo9I GATC 1 cut(s) 616
LmnI GCTCC 2 cut(s) 211, 257
Lsp1109I GCAGC 4 cut(s) 137, 490, 532, 878
MabI ACCWGGT 2 cut(s) 231, 1000
MaeI CTAG 3 cut(s) 620, 929, 1042
MaeII ACGT 1 cut(s) 934
MaeIII GTNAC 4 cut(s) 101, 134, 784, 944
MalI GATC 1 cut(s) 618
MbiI CCGCTC 1 cut(s) 252
MboI GATC 1 cut(s) 616
MboII GAAGA 5 cut(s) 4, 46, 373, 380, 452
MfeI CAATTG 1 cut(s) 527
MhlI GDGCHC 2 cut(s) 964, 1021
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 3 cut(s) 118, 144, 527
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 1 cut(s) 185
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 3 cut(s) 197, 917, 939
MslI CAYNNNNRTG 3 cut(s) 408, 566, 728
Msp20I TGGCCA 1 cut(s) 402
MspI CCGG 5 cut(s) 106, 114, 132, 191, 255
MspR9I CCNGG 6 cut(s) 114, 233, 343, 630, 774, 1002
MunI CAATTG 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 5 cut(s) 233, 343, 630, 774, 1002
MvnI CGCG 3 cut(s) 275, 482, 489
MwoI GCNNNNNNNGC 4 cut(s) 159, 264, 270, 989
NciI CCSGG 1 cut(s) 114
NcoI CCATGG 2 cut(s) 268, 403
NdeII GATC 1 cut(s) 616
NlaIII CATG 3 cut(s) 272, 289, 407
NlaIV GGNNCC 4 cut(s) 82, 213, 379, 380
NmuCI GTSAC 2 cut(s) 101, 944
NsbI TGCGCA 1 cut(s) 560
NspI RCATGY 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 728
PctI GAATGC 1 cut(s) 561
PfeI GAWTC 1 cut(s) 799
PflFI GACNNNGTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 845
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 8 cut(s) 152, 266, 312, 315, 491, 505, 547, 868
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 378
Psp6I CCWGG 5 cut(s) 231, 341, 628, 772, 1000
PspEI GGTNACC 1 cut(s) 944
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 5 cut(s) 231, 341, 628, 772, 1000
PspN4I GGNNCC 4 cut(s) 82, 213, 379, 380
PspPI GGNCC 3 cut(s) 247, 277, 378
PspPPI RGGWCCY 1 cut(s) 378
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 3 cut(s) 224, 476, 580
RsaNI GTAC 3 cut(s) 223, 475, 579
RseI CAYNNNNRTG 3 cut(s) 408, 566, 728
Rsr2I CGGWCCG 1 cut(s) 247
RsrII CGGWCCG 1 cut(s) 247
SaqAI TTAA 3 cut(s) 197, 917, 939
SatI GCNGC 8 cut(s) 151, 265, 311, 314, 490, 504, 546, 867
Sau3AI GATC 1 cut(s) 616
Sau96I GGNCC 3 cut(s) 247, 277, 378
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 6 cut(s) 114, 233, 343, 630, 774, 1002
SduI GDGCHC 2 cut(s) 964, 1021
SexAI ACCWGGT 2 cut(s) 231, 1000
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 3 cut(s) 247, 277, 378
SmiMI CAYNNNNRTG 3 cut(s) 408, 566, 728
Sse9I AATT 3 cut(s) 118, 144, 527
SsiI CCGC 9 cut(s) 250, 264, 275, 311, 314, 375, 489, 492, 592
SspMI CTAG 3 cut(s) 620, 929, 1042
StyD4I CCNGG 6 cut(s) 112, 231, 341, 628, 772, 1000
StyI CCWWGG 3 cut(s) 268, 403, 928
TaaI ACNGT 3 cut(s) 85, 247, 583
TaiI ACGT 1 cut(s) 937
TasI AATT 3 cut(s) 118, 144, 527
TatI WGTACW 1 cut(s) 222
TauI GCSGC 4 cut(s) 267, 313, 316, 492
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 3 cut(s) 197, 917, 939
Tru9I TTAA 3 cut(s) 197, 917, 939
TseFI GTSAC 2 cut(s) 101, 944
TseI GCWGC 4 cut(s) 150, 503, 545, 866
Tsp45I GTSAC 2 cut(s) 101, 944
TspDTI ATGAA 1 cut(s) 651
TspGWI ACGGA 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 3 cut(s) 247, 277, 378
XagI CCTNNNNNAGG 1 cut(s) 206
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XmaJI CCTAGG 1 cut(s) 928
XmiI GTMKAC 1 cut(s) 971
XspI CTAG 3 cut(s) 620, 929, 1042
ZraI GACGTC 1 cut(s) 935
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.