Rh6DG322600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0028919
Physical Location & Seq
Forward (+)
52789129 .. 52789876
748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG322600.1

Sequence Viewer

Length: 384 bp
ATGACCGGGGAGACTGGTACCCTTGGATACATGGCCCCAGAGGTCCTTGATGGTAAGCCTTACAACAGAAAATGTGACGTGTACAGTTTTGGTATTTGCTTATGGGAAATCTATTGTTGCGACATGCCTTATCCCAATCTTAGTTTTGCTGATGTCTCCTCTGCTGTCGTGCGACAGAATTTAAGACCTGAAATCCCAAGATGTTGTCCAACTGCGCTGGCTAGCATTATGCGAAAATGTTGGGATGCAAACCCAGATAAACGCCCCGACATGGAAGAAGTAGTAAAGTTATTGTATGCAGTCGACACAAGCAAGGGAGGTGGCATGATCCCGGAAGACCAAGCTTCTGCCTGTTCCTGTTTTGGCACACCTCGAGGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

127

Amino Acids

13.98

Weight (kDa)

4.9

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 97 1.5e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 7 - 97 6.8e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028919)

Species Orthologous Gene IDs
malus_domestica MD11G1033700.v1.1
rosa_samantha Rh6DG322600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 372
Acc65I GGTACC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 303
AclWI GGATC 1 cut(s) 322
AcsI RAATTY 1 cut(s) 178
AfaI GTAC 2 cut(s) 19, 83
AfiI CCNNNNNNNGG 1 cut(s) 271
AflIII ACRYGT 1 cut(s) 78
AjiI CACGTC 1 cut(s) 79
AluBI AGCT 1 cut(s) 344
AluI AGCT 1 cut(s) 344
Alw26I GTCTC 2 cut(s) 5, 160
AlwI GGATC 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 372
AoxI GGCC 2 cut(s) 33, 376
ApoI RAATTY 1 cut(s) 178
Asp718I GGTACC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 34, 43, 377
AsuC2I CCSGG 2 cut(s) 7, 332
AsuNHI GCTAGC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 342
BccI CCATC 1 cut(s) 44
BciVI GTATCC 1 cut(s) 20
BcnI CCSGG 2 cut(s) 7, 332
BcoDI GTCTC 2 cut(s) 5, 160
BfaI CTAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 20
Bme1390I CCNGG 2 cut(s) 7, 332
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 34, 43, 377
BmiI GGNNCC 2 cut(s) 19, 36
BmrFI CCNGG 2 cut(s) 7, 332
BmsI GCATC 1 cut(s) 235
BmtI GCTAGC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 7, 332
BsaJI CCNNGG 2 cut(s) 6, 22
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 6, 22
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 148
BshFI GGCC 2 cut(s) 35, 378
BshNI GGYRCC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 2 cut(s) 6, 332
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 2 cut(s) 5, 160
BsnI GGCC 2 cut(s) 35, 378
BsoBI CYCGRG 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 1 cut(s) 327
BspANI GGCC 2 cut(s) 35, 378
BspLI GGNNCC 2 cut(s) 19, 36
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 6, 22
BssMI GATC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 219, 223
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 330
BstMAI GTCTC 2 cut(s) 5, 160
BstMBI GATC 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 5, 330
BstV2I GAAGAC 1 cut(s) 342
BsuI GTATCC 1 cut(s) 20
BsuRI GGCC 2 cut(s) 35, 378
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 219, 223
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 34, 43, 377
Csp6I GTAC 2 cut(s) 18, 82
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 4 cut(s) 31, 124, 271, 325
CviJI RGCY 5 cut(s) 35, 58, 221, 344, 378
CviKI_1 RGCY 5 cut(s) 35, 58, 221, 344, 378
CviQI GTAC 2 cut(s) 18, 82
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 372
EcoO109I RGGNCCY 2 cut(s) 43, 377
EcoT14I CCWWGG 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 4 cut(s) 34, 127, 274, 328
FaiI YATR 7 cut(s) 32, 103, 125, 230, 272, 297, 326
FatI CATG 4 cut(s) 30, 123, 270, 324
FblI GTMKAC 1 cut(s) 303
FokI GGATG 1 cut(s) 257
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 216
HaeIII GGCC 2 cut(s) 35, 378
HapII CCGG 2 cut(s) 6, 332
HhaI GCGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 34, 127, 274, 328
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 342
HpaII CCGG 2 cut(s) 6, 332
Hpy166II GTNNAC 2 cut(s) 82, 304
Hpy8I GTNNAC 2 cut(s) 82, 304
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 2 cut(s) 248, 299
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 4 cut(s) 34, 127, 274, 328
HspAI GCGC 1 cut(s) 215
KpnI GGTACC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 327
LpnPI CCDG 8 cut(s) 19, 51, 201, 203, 267, 345, 364, 370
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 74
MalI GATC 1 cut(s) 329
MboI GATC 1 cut(s) 327
MboII GAAGA 2 cut(s) 287, 347
MluCI AATT 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 5 cut(s) 34, 169, 311, 368, 381
MseI TTAA 1 cut(s) 182
MspI CCGG 2 cut(s) 6, 332
MspR9I CCNGG 2 cut(s) 7, 332
NciI CCSGG 2 cut(s) 7, 332
NdeII GATC 1 cut(s) 327
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 4 cut(s) 34, 127, 274, 328
NlaIV GGNNCC 2 cut(s) 19, 36
NmuCI GTSAC 1 cut(s) 74
NspI RCATGY 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 372
PfoI TCCNGGA 1 cut(s) 330
PpuMI RGGWCCY 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 19, 36
PspPI GGNCC 3 cut(s) 34, 43, 377
PspPPI RGGWCCY 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 372
RsaI GTAC 2 cut(s) 19, 83
RsaNI GTAC 2 cut(s) 18, 82
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 182
Sau3AI GATC 1 cut(s) 327
Sau96I GGNCC 3 cut(s) 34, 43, 377
ScrFI CCNGG 2 cut(s) 7, 332
SetI ASST 6 cut(s) 45, 81, 190, 322, 346, 373
SfaNI GCATC 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 43
SlaI CTCGAG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 372
SmoI CTYRAG 1 cut(s) 372
Sse9I AATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 5, 330
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 303, 373
TasI AATT 1 cut(s) 178
TatI WGTACW 1 cut(s) 81
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 178
XceI RCATGY 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 372
XmiI GTMKAC 1 cut(s) 303
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.