Rh7BG290100

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0032459
Physical Location & Seq
Forward (+)
27233483 .. 27234088
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG290100.1

Sequence Viewer

Length: 606 bp
ATGAGCAAAGTTGGCCAAGATTCGTACCTGGCCATTCTGTTTGAATCTGGACGTGACGAGGTGAACGTCAGTCACATTGGCGTGTTAAAGCACCATACTGAAACGGGCAAGGGTCGGCCCGAGCCACGTTATGTTGCTCTAGATCTTGACCGTACAAGTGGGAGAGAGGTGACCGCTTGGGTTGAATACAGAAACGACCAGAAGGCCTTTCGGGTTTGGATCGAGTACTCGCCCCAGAACCTACCTCCGAGTCCAGCTCTTGAGCTCGAGATCGACCTCTCCAAGGCATCTAAGGAATTAGCCCATGTGGGTTTCAGTGCTAACCCGGCGGGTTCTGCTGCTTATCTGTTGACATATTGGAGCTTCCAGGCTTTTTTTTACCGCCTCCCTTCCTCAATCCCTTTCAGCTTTTGGGAGCATATTGACTGTGTGTATTGCAACATAGAGCGTTCGGGTCATAGCCAAAGACGCACAACAAGCATCCTGATTGCTCTCGTTTCTGTACTACTAGTGCTTCTTGCGACGCTACTTTGCCTACTCCTTTCAAAGAAGAGAAACGTGAGTCCCAGAAGAAGGGGGACCATGTCCCAGGTCCAGATGACATAG

Protein Analysis

201

Amino Acids

22.76

Weight (kDa)

9.01

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 20 - 123 8.7e-11 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0032459)

Species Orthologous Gene IDs
rosa_rugosa Rorug07G0160300
rosa_samantha Rh7BG290100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 174, 329, 382
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 2 cut(s) 13, 30
AfaI GTAC 4 cut(s) 26, 154, 227, 504
AfiI CCNNNNNNNGG 2 cut(s) 283, 573
AgsI TTSAA 3 cut(s) 44, 185, 546
AhlI ACTAGT 1 cut(s) 508
AjiI CACGTC 1 cut(s) 53
AjnI CCWGG 3 cut(s) 27, 366, 588
AluBI AGCT 4 cut(s) 257, 265, 363, 408
AluI AGCT 4 cut(s) 257, 265, 363, 408
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 2 cut(s) 119, 266
AoxI GGCC 4 cut(s) 13, 30, 116, 204
ApeKI GCWGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 117, 579, 592
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 73, 181
AvaI CYCGRG 2 cut(s) 119, 266
AvaII GGWCC 2 cut(s) 579, 592
BalI TGGCCA 2 cut(s) 15, 32
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 325
BciT130I CCWGG 3 cut(s) 29, 368, 590
BcnI CCSGG 1 cut(s) 326
BcuI ACTAGT 1 cut(s) 508
BfaI CTAG 2 cut(s) 140, 509
BglII AGATCT 1 cut(s) 142
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 4 cut(s) 29, 326, 368, 590
Bme18I GGWCC 2 cut(s) 579, 592
BmeT110I CYCGRG 2 cut(s) 119, 266
BmgBI CACGTC 1 cut(s) 53
BmgT120I GGNCC 3 cut(s) 117, 579, 592
BmiI GGNNCC 1 cut(s) 580
BmrFI CCNGG 4 cut(s) 29, 326, 368, 590
BmsI GCATC 2 cut(s) 296, 489
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 282, 588
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 573
BseBI CCWGG 3 cut(s) 29, 368, 590
BseDI CCNNGG 2 cut(s) 282, 588
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 2 cut(s) 283, 573
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 4 cut(s) 15, 32, 118, 206
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 119, 266
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 3 cut(s) 549, 571, 592
BslI CCNNNNNNNGG 2 cut(s) 283, 573
BsmFI GGGAC 3 cut(s) 549, 571, 592
BsnI GGCC 4 cut(s) 15, 32, 118, 206
BsoBI CYCGRG 2 cut(s) 119, 266
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 3 cut(s) 142, 219, 270
BspACI CCGC 3 cut(s) 174, 329, 382
BspANI GGCC 4 cut(s) 15, 32, 118, 206
BspLI GGNNCC 1 cut(s) 580
BspPI GGATC 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 282, 588
BssMI GATC 3 cut(s) 142, 219, 270
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 3 cut(s) 29, 368, 590
Bst4CI ACNGT 2 cut(s) 152, 428
Bst6I CTCTTC 1 cut(s) 545
BstDEI CTNAG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 169
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 480
BstKTI GATC 3 cut(s) 145, 222, 273
BstMBI GATC 3 cut(s) 142, 219, 270
BstMWI GCNNNNNNNGC 5 cut(s) 12, 326, 335, 468, 477
BstNI CCWGG 3 cut(s) 29, 368, 590
BstPI GGTNACC 1 cut(s) 169
BstSCI CCNGG 4 cut(s) 27, 324, 366, 588
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuRI GGCC 4 cut(s) 15, 32, 118, 206
BtrI CACGTC 1 cut(s) 53
BtsCI GGATG 1 cut(s) 480
BtsIMutI CAGTG 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 117, 579, 592
CseI GACGC 2 cut(s) 477, 532
Csp6I GTAC 4 cut(s) 25, 153, 226, 503
CviAII CATG 2 cut(s) 305, 583
CviQI GTAC 4 cut(s) 25, 153, 226, 503
DdeI CTNAG 1 cut(s) 291
DpnI GATC 3 cut(s) 144, 221, 272
DpnII GATC 3 cut(s) 142, 219, 270
EaeI YGGCCR 2 cut(s) 13, 30
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 579, 592
Eco53kI GAGCTC 1 cut(s) 265
Eco88I CYCGRG 2 cut(s) 119, 266
Eco91I GGTNACC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoO65I GGTNACC 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 27, 366, 588
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 308, 586
FaiI YATR 9 cut(s) 96, 132, 306, 355, 420, 443, 459, 584, 604
FaqI GGGAC 3 cut(s) 549, 571, 592
FatI CATG 2 cut(s) 304, 582
FauI CCCGC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 2 cut(s) 140, 509
GluI GCNGC 1 cut(s) 339
HaeIII GGCC 4 cut(s) 15, 32, 118, 206
HapII CCGG 1 cut(s) 326
HgaI GACGC 2 cut(s) 477, 532
Hin1II CATG 2 cut(s) 308, 586
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 4 cut(s) 20, 44, 250, 562
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 73, 181
Hpy166II GTNNAC 2 cut(s) 64, 351
Hpy188I TCNGA 1 cut(s) 249
Hpy188III TCNNGA 7 cut(s) 48, 140, 146, 260, 268, 484, 595
Hpy8I GTNNAC 2 cut(s) 64, 351
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 3 cut(s) 196, 399, 567
HpyCH4III ACNGT 2 cut(s) 152, 428
HpyCH4IV ACGT 4 cut(s) 52, 66, 127, 558
HpyCH4V TGCA 1 cut(s) 438
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 326, 335, 468, 477
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 4 cut(s) 52, 66, 127, 558
Hsp92II CATG 2 cut(s) 308, 586
Kzo9I GATC 3 cut(s) 142, 219, 270
LmnI GCTCC 2 cut(s) 360, 415
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 2 cut(s) 296, 489
MaeI CTAG 2 cut(s) 140, 509
MaeII ACGT 4 cut(s) 52, 66, 127, 558
MaeIII GTNAC 3 cut(s) 53, 71, 169
MalI GATC 3 cut(s) 144, 221, 272
MboI GATC 3 cut(s) 142, 219, 270
MboII GAAGA 2 cut(s) 562, 582
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 2 cut(s) 15, 32
MluCI AATT 1 cut(s) 296
MluNI TGGCCA 2 cut(s) 15, 32
MlyI GAGTC 2 cut(s) 259, 571
MnlI CCTC 6 cut(s) 52, 160, 255, 287, 395, 403
Mox20I TGGCCA 2 cut(s) 15, 32
MscI TGGCCA 2 cut(s) 15, 32
MseI TTAA 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 2 cut(s) 15, 32
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 4 cut(s) 29, 326, 368, 590
MvaI CCWGG 3 cut(s) 29, 368, 590
MwoI GCNNNNNNNGC 5 cut(s) 12, 326, 335, 468, 477
NciI CCSGG 1 cut(s) 326
NdeII GATC 3 cut(s) 142, 219, 270
NlaIII CATG 2 cut(s) 308, 586
NlaIV GGNNCC 1 cut(s) 580
NmuCI GTSAC 3 cut(s) 53, 71, 169
PaeR7I CTCGAG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 20, 44
PflFI GACNNNGTC 1 cut(s) 583
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 2 cut(s) 258, 570
PpsI GAGTC 2 cut(s) 258, 570
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 3 cut(s) 27, 366, 588
PspEI GGTNACC 1 cut(s) 169
PspGI CCWGG 3 cut(s) 27, 366, 588
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 3 cut(s) 117, 579, 592
PsuI RGATCY 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 583
RsaI GTAC 4 cut(s) 26, 154, 227, 504
RsaNI GTAC 4 cut(s) 25, 153, 226, 503
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 3 cut(s) 142, 219, 270
Sau96I GGNCC 3 cut(s) 117, 579, 592
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 2 cut(s) 259, 571
ScrFI CCNGG 4 cut(s) 29, 326, 368, 590
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 2 cut(s) 296, 489
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 2 cut(s) 579, 592
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 2 cut(s) 260, 266
SmoI CTYRAG 2 cut(s) 260, 266
SpeI ACTAGT 1 cut(s) 508
Sse9I AATT 1 cut(s) 296
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 3 cut(s) 174, 329, 382
SspMI CTAG 2 cut(s) 140, 509
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 4 cut(s) 27, 324, 366, 588
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 152, 428
TaiI ACGT 4 cut(s) 55, 69, 130, 561
TaqI TCGA 3 cut(s) 222, 267, 273
TasI AATT 1 cut(s) 296
TatI WGTACW 2 cut(s) 225, 502
TfiI GAWTC 2 cut(s) 20, 44
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 3 cut(s) 53, 71, 169
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 3 cut(s) 53, 71, 169
TspRI CASTG 1 cut(s) 322
Tth111I GACNNNGTC 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 579, 592
XagI CCTNNNNNAGG 1 cut(s) 281
XbaI TCTAGA 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 266
XspI CTAG 2 cut(s) 140, 509
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.