Rh7CG249800

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0025526
Physical Location & Seq
Forward (+)
23579640 .. 23579858
219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG249800.1

Sequence Viewer

Length: 219 bp
ATGAGTGATGATTTCATCGAACAATCTTGCAGGATAGCTGCTGGTCGTGAACCATCTTTGAACTACACCTTCTGTGTCTCAACTCTTAAGGCCAGCCCTAAAAGCCATGGTGCAGACCTTGATGGACTCATGTGCATCGTACTTCGCCTAGCCATAGACAAGGTGACCTACATCAATGACCTCATCCAAAAGTTGGTGAAGGATCCGACAGTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

72

Amino Acids

7.95

Weight (kDa)

5.14

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 68 5e-06 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025526)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002039.1_g000004
rosa_samantha Rh7CG249800 Rh7DG240800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 193
AccI GTMKAC 1 cut(s) 213
AclWI GGATC 2 cut(s) 197, 210
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 1 cut(s) 193
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 2 cut(s) 197, 210
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 175, 208
BamHI GGATCC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 25
BccI CCATC 2 cut(s) 61, 116
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 149
BfrI CTTAAG 1 cut(s) 86
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 204
BmsI GCATC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 1 cut(s) 193
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 202
Bsp19I CCATGG 1 cut(s) 106
BspANI GGCC 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 2 cut(s) 197, 210
BspTI CTTAAG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 1 cut(s) 202
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 94
BstDSI CCRYGG 1 cut(s) 106
BstEII GGTNACC 1 cut(s) 163
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 140
CviAII CATG 2 cut(s) 107, 130
CviJI RGCY 5 cut(s) 38, 92, 96, 105, 152
CviKI_1 RGCY 5 cut(s) 38, 92, 96, 105, 152
CviQI GTAC 1 cut(s) 140
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 110, 133
FaiI YATR 3 cut(s) 108, 131, 155
FatI CATG 2 cut(s) 106, 129
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 1 cut(s) 149
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 92
Hin1II CATG 2 cut(s) 110, 133
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 1 cut(s) 126
HphI GGTGA 2 cut(s) 175, 208
Hpy166II GTNNAC 2 cut(s) 50, 214
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 2 cut(s) 50, 214
HpyAV CCTTC 2 cut(s) 79, 193
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 3 cut(s) 30, 113, 135
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 110, 133
Kzo9I GATC 1 cut(s) 202
LpnPI CCDG 3 cut(s) 16, 27, 106
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 1 cut(s) 144
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 163
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MflI RGATCY 1 cut(s) 202
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 1 cut(s) 191
MseI TTAA 1 cut(s) 87
MspCI CTTAAG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 102
NcoI CCATGG 1 cut(s) 106
NdeII GATC 1 cut(s) 202
NlaIII CATG 2 cut(s) 110, 133
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 163
PflMI CCANNNNNTGG 1 cut(s) 193
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 204
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 1 cut(s) 202
SchI GAGTC 1 cut(s) 120
SetI ASST 6 cut(s) 40, 71, 120, 165, 170, 183
SfaNI GCATC 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SspMI CTAG 1 cut(s) 149
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 2 cut(s) 18, 213
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 4
Van91I CCANNNNNTGG 1 cut(s) 193
Vha464I CTTAAG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.