Rh7CG459700

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0031117
Physical Location & Seq
Forward (+)
60799998 .. 60832568
32571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG459700.1

Sequence Viewer

Length: 648 bp
ATGCCTAGCCAAGGGAATTCGCACTATAGCAATGGTGGCCTGGAAAGAGAGTCCAACAATCCATACCATCGGCAAGCAGTAACTGACATAAATTCGAACAAAAATGTGAATGAGAAACAAAGACTCTCAACAGAGTCATCACGGGCCTCTTTCTCGTCGACTTGTTCATCTTCCTTATCATCACTGGAGTGTAACAGAACAGCACAACCAGGAACCTCTTCCTTTGACAGAATCATTTTTCCTGAAACTCCGCCAAGGGATCCAGTAACTAACCACTCAAGTACCTCCCCTAGATTTGGAAGGCAGTCCCTTGATCTCCGGGATGTGCCAAGTCCATTTTTCACTGAGCAGTTGATTGCTTTTGAGGTATGGCCTGACCACGGGTCTGAACACAAGAAATCACCAGAGCAGTTGCCTATTGTTCTTCAGGGAAGGAAGGGAATTTGGCTTAAGCTACTGGTAGAACGAGCTGAGCTTGTTCCAGTTGCTGTTGAGCCATGGCGACCGCTGCGTTGCTCCGATCTCTACGACGTCATGACCTCGCTTCAGCCCCTCTGTTCCTACTGCTTGCCGAAGTACCGATCTAGTGTCACTGATCAAATTTCAAAAAGAGAGATCGCAAAAGCTCTTTCTATATCCAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

215

Amino Acids

24.15

Weight (kDa)

8.29

Isoelectric Point (pI)

64.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031117)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001142.1_g000009
rosa_samantha Rh7CG459700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 534
AccI GTMKAC 1 cut(s) 158
AciI CCGC 2 cut(s) 251, 506
AclWI GGATC 2 cut(s) 254, 267
AcsI RAATTY 4 cut(s) 16, 91, 441, 600
AcuI CTGAAG 2 cut(s) 410, 530
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 2 cut(s) 283, 578
AfiI CCNNNNNNNGG 3 cut(s) 11, 296, 380
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 1 cut(s) 606
AhdI GACNNNNNGTC 1 cut(s) 382
AjnI CCWGG 2 cut(s) 39, 208
AleI CACNNNNGTG 1 cut(s) 187
AluBI AGCT 4 cut(s) 454, 470, 475, 626
AluI AGCT 4 cut(s) 454, 470, 475, 626
AlwI GGATC 2 cut(s) 254, 267
AlwNI CAGNNNCTG 2 cut(s) 83, 488
AoxI GGCC 3 cut(s) 37, 144, 371
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 4 cut(s) 16, 91, 441, 600
Asp700I GAANNNNTTC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 144
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 393
AsuII TTCGAA 1 cut(s) 95
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BamHI GGATCC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 495
BccI CCATC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 41, 210
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 320
BfaI CTAG 3 cut(s) 6, 291, 585
BfmI CTRYAG 1 cut(s) 25
BfrI CTTAAG 1 cut(s) 449
BisI GCNGC 1 cut(s) 509
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 510
Bme1390I CCNGG 3 cut(s) 41, 210, 320
BmeRI GACNNNNNGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 2 cut(s) 214, 261
BmrFI CCNGG 3 cut(s) 41, 210, 320
BpmI CTGGAG 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 471
Bpu14I TTCGAA 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 4 cut(s) 10, 254, 379, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 296, 380
Bse1I ACTGG 4 cut(s) 189, 263, 462, 482
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 41, 210
BseDI CCNNGG 4 cut(s) 10, 254, 379, 497
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 3 cut(s) 11, 296, 380
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 336, 462
BseNI ACTGG 4 cut(s) 189, 263, 462, 482
BseXI GCAGC 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 3 cut(s) 39, 146, 373
BsiEI CGRYCG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 3 cut(s) 11, 296, 380
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 3 cut(s) 39, 146, 373
Bsp119I TTCGAA 1 cut(s) 95
Bsp143I GATC 6 cut(s) 259, 313, 520, 581, 595, 615
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 497
BspACI CCGC 2 cut(s) 251, 506
BspANI GGCC 3 cut(s) 39, 146, 373
BspCNI CTCAG 2 cut(s) 337, 463
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 2 cut(s) 214, 261
BspPI GGATC 2 cut(s) 254, 267
BspT104I TTCGAA 1 cut(s) 95
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 4 cut(s) 189, 263, 462, 482
BssECI CCNNGG 4 cut(s) 10, 254, 379, 497
BssMI GATC 6 cut(s) 259, 313, 520, 581, 595, 615
BssNI GRCGYC 1 cut(s) 531
BssT1I CCWWGG 3 cut(s) 10, 254, 497
Bst2UI CCWGG 2 cut(s) 41, 210
Bst6I CTCTTC 1 cut(s) 223
BstACI GRCGYC 1 cut(s) 531
BstAFI CTTAAG 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 75, 569
BstDEI CTNAG 2 cut(s) 345, 471
BstDSI CCRYGG 2 cut(s) 379, 497
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 6 cut(s) 262, 316, 523, 584, 598, 618
BstMBI GATC 6 cut(s) 259, 313, 520, 581, 595, 615
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 2 cut(s) 36, 508
BstNI CCWGG 2 cut(s) 41, 210
BstSCI CCNGG 3 cut(s) 39, 208, 318
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 3 cut(s) 39, 146, 373
BtgI CCRYGG 2 cut(s) 379, 497
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 182, 342, 591
Cac8I GCNNGC 2 cut(s) 75, 569
CaiI CAGNNNCTG 2 cut(s) 83, 488
CciI TCATGA 1 cut(s) 534
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 282, 577
CviAII CATG 2 cut(s) 498, 535
CviQI GTAC 2 cut(s) 282, 577
DdeI CTNAG 2 cut(s) 345, 471
DpnI GATC 6 cut(s) 261, 315, 522, 583, 597, 617
DpnII GATC 6 cut(s) 259, 313, 520, 581, 595, 615
DriI GACNNNNNGTC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 223
Eam1105I GACNNNNNGTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 3 cut(s) 10, 254, 497
Eco57I CTGAAG 2 cut(s) 410, 530
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 39, 208
EcoT14I CCWWGG 3 cut(s) 10, 254, 497
ErhI CCWWGG 3 cut(s) 10, 254, 497
FaeI CATG 2 cut(s) 501, 538
FaiI YATR 7 cut(s) 27, 64, 89, 370, 499, 536, 635
FaqI GGGAC 1 cut(s) 292
FatI CATG 2 cut(s) 497, 534
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 1 cut(s) 509
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 1 cut(s) 509
FspBI CTAG 3 cut(s) 6, 291, 585
GluI GCNGC 1 cut(s) 509
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 3 cut(s) 39, 146, 373
HapII CCGG 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 2 cut(s) 501, 538
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 4 cut(s) 50, 123, 134, 231
HpaII CCGG 1 cut(s) 319
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 2 cut(s) 388, 520
Hpy188III TCNNGA 2 cut(s) 242, 535
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 2 cut(s) 160, 533
HpyAV CCTTC 3 cut(s) 294, 426, 430
HpyCH4IV ACGT 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 508
HpyF3I CTNAG 2 cut(s) 345, 471
HpySE526I ACGT 1 cut(s) 531
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 2 cut(s) 501, 538
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 259, 313, 520, 581, 595, 615
LmnI GCTCC 1 cut(s) 521
Lsp1109I GCAGC 1 cut(s) 495
MaeI CTAG 3 cut(s) 6, 291, 585
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 4 cut(s) 79, 191, 265, 589
MalI GATC 6 cut(s) 261, 315, 522, 583, 597, 617
MboI GATC 6 cut(s) 259, 313, 520, 581, 595, 615
MboII GAAGA 3 cut(s) 162, 210, 416
MflI RGATCY 1 cut(s) 259
MluCI AATT 5 cut(s) 16, 91, 441, 600, 643
MlyI GAGTC 3 cut(s) 59, 117, 143
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 6 cut(s) 157, 226, 295, 358, 550, 563
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 2 cut(s) 450, 646
MslI CAYNNNNRTG 1 cut(s) 187
MspA1I CMGCKG 1 cut(s) 508
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 319
MspR9I CCNGG 3 cut(s) 41, 210, 320
MvaI CCWGG 2 cut(s) 41, 210
MwoI GCNNNNNNNGC 2 cut(s) 36, 508
NciI CCSGG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 497
NdeII GATC 6 cut(s) 259, 313, 520, 581, 595, 615
NlaIII CATG 2 cut(s) 501, 538
NlaIV GGNNCC 2 cut(s) 214, 261
NmuCI GTSAC 1 cut(s) 589
NspV TTCGAA 1 cut(s) 95
OliI CACNNNNGTG 1 cut(s) 187
PagI TCATGA 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 1 cut(s) 510
PleI GAGTC 3 cut(s) 58, 117, 142
PpsI GAGTC 3 cut(s) 58, 117, 142
Psp6I CCWGG 2 cut(s) 39, 208
PspGI CCWGG 2 cut(s) 39, 208
PspN4I GGNNCC 2 cut(s) 214, 261
PspPI GGNCC 1 cut(s) 144
PstNI CAGNNNCTG 2 cut(s) 83, 488
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 2 cut(s) 283, 578
RsaNI GTAC 2 cut(s) 282, 577
RseI CAYNNNNRTG 1 cut(s) 187
SalI GTCGAC 1 cut(s) 157
SaqAI TTAA 2 cut(s) 450, 646
SatI GCNGC 1 cut(s) 509
Sau3AI GATC 6 cut(s) 259, 313, 520, 581, 595, 615
Sau96I GGNCC 1 cut(s) 144
SchI GAGTC 3 cut(s) 59, 117, 143
ScrFI CCNGG 3 cut(s) 41, 210, 320
SetI ASST 9 cut(s) 218, 287, 369, 456, 472, 477, 534, 542, 628
SfcI CTRYAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 187
SmlI CTYRAG 2 cut(s) 277, 449
SmoI CTYRAG 2 cut(s) 277, 449
Sse9I AATT 5 cut(s) 16, 91, 441, 600, 643
SsiI CCGC 2 cut(s) 251, 506
SspMI CTAG 3 cut(s) 6, 291, 585
StyD4I CCNGG 3 cut(s) 39, 208, 318
StyI CCWWGG 3 cut(s) 10, 254, 497
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 95, 158
TasI AATT 5 cut(s) 16, 91, 441, 600, 643
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 2 cut(s) 450, 646
Tru9I TTAA 2 cut(s) 450, 646
TscAI CASTG 3 cut(s) 189, 349, 598
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 3 cut(s) 189, 349, 598
Vha464I CTTAAG 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 4 cut(s) 16, 91, 441, 600
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 3 cut(s) 6, 291, 585
ZraI GACGTC 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.