Rh7DG017400

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0033173
Physical Location & Seq
Forward (+)
1411028 .. 1415602
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG017400.1

Sequence Viewer

Length: 1188 bp
ATGGGGAGTGGAGCAACCTTGGTGGATGGTGTTCGTCGCTGGTTTCAACGTCGTACCTCTTCCTCTTCCAAACCTATTAATCCTGCTACTAGTACTAACACTAATCCATACCCGAATCCGAATAATAACAACCATGATTATAATGCGAGCGATTTAAGCGCGCAATCGTCCGCTGGTCTCCAAAAAGAGGAGGACGACCGTCTTCTTCAGTTTCAGATTGAGGACGAGTTCGACATCTCTGGTTTGAAGCTCATCCAATATACTCCTGCAATTGATATCTGGAGCATAGGATGCATATTTGCAGAAATACTCACTGGCAAACCCCTGTTTCCTGGGAAAAACGTGGTGCACCAATTGGATCTCATGACTGATTTGCTTGGCACGCCTTCTTCAGAATCCATTGCAAGGATTCGGAATGAAAAAGCAAGAAGGTATCTTAGTAGCATGCGAAAAAAACAACCAGTTCCCTTCACACATAAGTTCCCTAATGCAGATCCGTTGGCTCTTCGCCTGTTGGAGCAACTTCTTGCATTTGATCCCAAAGATCGTCCGACAGCTGAAGAGGCATTAGCTGATCCATACTTTCATGGTTTGGCAAATGTTGATCGTGAGCCGTCCACTCAACCCATTTCAAAACTTGAATTTGAGTTTGAGCGAAGGAAATTGACAAAAGATGATGTCAGAGAGTTGATTTACAGGGAGATTTTAGAGTATCATCCACAGATGCTGCAGGAGTATCTTCGAGGGGGAGATCAAACGAGCAGCTTTATGTATCCTAGTGGTGTTGATCGATTCAAGCGACAGTTTGCACATCTTGAGGAACACTATGGTAAAGGTGAAAGGGGCACTCCTCCACTCCAAAGGCAGCATGCCTCCTTGCCCAGAGAGCGGGTTCCTGCACCCAAGGAGGATGCTGACCAAAGCAATGAATCGGAAAGGACTGCCGCTTCTGTTGCCACAACTCTTCACAGCCCTCCATCTGATGGCTCTGATAATCCAAATGCAAATGGACCTAGCAAGACACAGTACACTCCAGTTTCTAGTCGCAGCCTGTTGAAGAGTGCCAGCATCAGTGCTTCCAAGTGTATAGGTGTAGTGAAAAAAGATTCACAGGAGGATCCAATTGCTGAGGTCAACGATGAGTCAGTTGATGGTTTGGCCCAAAAGGTCGAAGCTCTTCGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

395

Amino Acids

44.13

Weight (kDa)

5.81

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 86 - 195 1e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033173)

Species Orthologous Gene IDs
rosa_samantha Rh7BG016800 Rh7DG017400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 983
AccBSI CCGCTC 1 cut(s) 889
AccII CGCG 1 cut(s) 161
AciI CCGC 3 cut(s) 171, 889, 945
AclWI GGATC 6 cut(s) 366, 488, 530, 569, 1112, 1125
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 3 cut(s) 191, 375, 579
AfaI GTAC 3 cut(s) 55, 94, 1028
AfiI CCNNNNNNNGG 4 cut(s) 187, 405, 888, 983
AgsI TTSAA 6 cut(s) 47, 247, 633, 641, 796, 1057
AhlI ACTAGT 1 cut(s) 89
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 5 cut(s) 250, 557, 572, 765, 1175
AluI AGCT 5 cut(s) 250, 557, 572, 765, 1175
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 347
AlwI GGATC 6 cut(s) 366, 488, 530, 569, 1112, 1125
AlwNI CAGNNNCTG 1 cut(s) 727
AoxI GGCC 1 cut(s) 1158
ApaLI GTGCAC 1 cut(s) 347
ApeKI GCWGC 4 cut(s) 727, 762, 865, 1047
ApoI RAATTY 1 cut(s) 641
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 1 cut(s) 1176
AspLEI GCGC 2 cut(s) 161, 163
AspS9I GGNCC 2 cut(s) 1010, 1159
AsuHPI GGTGA 1 cut(s) 848
AvaII GGWCC 1 cut(s) 1010
BaeGI GKGCMC 2 cut(s) 351, 848
BamHI GGATCC 1 cut(s) 1117
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1128
BbvI GCAGC 4 cut(s) 714, 774, 877, 1059
BccI CCATC 4 cut(s) 20, 977, 985, 1145
BceAI ACGGC 1 cut(s) 598
BciT130I CCWGG 1 cut(s) 333
BciVI GTATCC 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 182
BcuI ACTAGT 1 cut(s) 89
BfaI CTAG 4 cut(s) 90, 777, 1014, 1041
BfmI CTRYAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 783
BisI GCNGC 5 cut(s) 728, 763, 866, 945, 1048
BlsI GCNGC 5 cut(s) 729, 764, 867, 946, 1049
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 1010
BmgT120I GGNCC 2 cut(s) 1010, 1159
BmiI GGNNCC 2 cut(s) 894, 1119
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 281, 714, 901, 1077
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 2 cut(s) 301, 1017
Bpu10I CCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 836
Bsa29I ATCGAT 1 cut(s) 790
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 18, 332, 903
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 405, 888, 983
Bse1I ACTGG 3 cut(s) 319, 461, 1034
Bse3DI GCAATG 2 cut(s) 399, 931
BseBI CCWGG 1 cut(s) 333
BseCI ATCGAT 1 cut(s) 790
BseDI CCNNGG 3 cut(s) 18, 332, 903
BseGI GGATG 5 cut(s) 31, 252, 296, 715, 916
BseLI CCNNNNNNNGG 4 cut(s) 187, 405, 888, 983
BseMI GCAATG 2 cut(s) 399, 931
BseMII CTCAG 1 cut(s) 1119
BseNI ACTGG 3 cut(s) 319, 461, 1034
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 2 cut(s) 203, 840
BseSI GKGCMC 2 cut(s) 351, 848
BseXI GCAGC 4 cut(s) 714, 774, 877, 1059
BsgI GTGCAG 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 1 cut(s) 1160
BshVI ATCGAT 1 cut(s) 790
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 351
BslI CCNNNNNNNGG 4 cut(s) 187, 405, 888, 983
BsmAI GTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 1160
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 351, 848
Bsp143I GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
BspACI CCGC 3 cut(s) 171, 889, 945
BspANI GGCC 1 cut(s) 1160
BspCNI CTCAG 1 cut(s) 1120
BspDI ATCGAT 1 cut(s) 790
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 2 cut(s) 894, 1119
BspMAI CTGCAG 1 cut(s) 732
BspPI GGATC 6 cut(s) 366, 488, 530, 569, 1112, 1125
BspQI GCTCTTC 2 cut(s) 510, 1182
BspTNI GGTCTC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 399, 931
BsrI ACTGG 3 cut(s) 319, 461, 1034
BssECI CCNNGG 3 cut(s) 18, 332, 903
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
BssT1I CCWWGG 2 cut(s) 18, 903
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 3 cut(s) 200, 804, 1026
Bst6I CTCTTC 7 cut(s) 64, 70, 510, 555, 969, 1052, 1182
BstAPI GCANNNNNTGC 1 cut(s) 291
BstC8I GCNNGC 6 cut(s) 148, 161, 383, 446, 870, 1066
BstDEI CTNAG 2 cut(s) 437, 1128
BstF5I GGATG 5 cut(s) 31, 252, 296, 715, 916
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 2 cut(s) 161, 163
BstKTI GATC 9 cut(s) 361, 496, 538, 547, 577, 607, 754, 790, 1120
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 7 cut(s) 156, 291, 382, 563, 886, 953, 1181
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 2 cut(s) 448, 872
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 2 cut(s) 351, 848
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 4 cut(s) 714, 774, 877, 1059
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 3 cut(s) 358, 493, 1117
BstYI RGATCY 3 cut(s) 358, 493, 1117
Bsu15I ATCGAT 1 cut(s) 790
BsuI GTATCC 1 cut(s) 783
BsuRI GGCC 1 cut(s) 1160
BsuTUI ATCGAT 1 cut(s) 790
BtsCI GGATG 5 cut(s) 31, 252, 296, 715, 916
BtsIMutI CAGTG 2 cut(s) 312, 1078
Cac8I GCNNGC 6 cut(s) 148, 161, 383, 446, 870, 1066
CaiI CAGNNNCTG 1 cut(s) 727
CciI TCATGA 1 cut(s) 363
CfoI GCGC 2 cut(s) 161, 163
Cfr13I GGNCC 2 cut(s) 1010, 1159
ClaI ATCGAT 1 cut(s) 790
Csp6I GTAC 3 cut(s) 54, 93, 1027
CspCI CAANNNNNGTGG 1 cut(s) 38
CviAII CATG 5 cut(s) 134, 364, 445, 587, 869
CviQI GTAC 3 cut(s) 54, 93, 1027
DdeI CTNAG 2 cut(s) 437, 1128
DpnI GATC 9 cut(s) 360, 495, 537, 546, 576, 606, 753, 789, 1119
DpnII GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
Eam1104I CTCTTC 7 cut(s) 64, 70, 510, 555, 969, 1052, 1182
EarI CTCTTC 7 cut(s) 64, 70, 510, 555, 969, 1052, 1182
Eco130I CCWWGG 2 cut(s) 18, 903
Eco31I GGTCTC 1 cut(s) 182
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 1010
Eco57I CTGAAG 3 cut(s) 191, 375, 579
EcoRII CCWGG 1 cut(s) 331
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 18, 903
EcoT22I ATGCAT 1 cut(s) 296
ErhI CCWWGG 2 cut(s) 18, 903
FaeI CATG 5 cut(s) 137, 367, 448, 590, 872
FatI CATG 5 cut(s) 133, 363, 444, 586, 868
FauI CCCGC 1 cut(s) 882
Fnu4HI GCNGC 5 cut(s) 728, 763, 866, 945, 1048
FokI GGATG 5 cut(s) 38, 239, 303, 702, 923
Fsp4HI GCNGC 5 cut(s) 728, 763, 866, 945, 1048
FspBI CTAG 4 cut(s) 90, 777, 1014, 1041
GlaI GCGC 2 cut(s) 160, 162
GluI GCNGC 5 cut(s) 728, 763, 866, 945, 1048
GsuI CTGGAG 2 cut(s) 301, 1017
HaeIII GGCC 1 cut(s) 1160
HhaI GCGC 2 cut(s) 161, 163
Hin1II CATG 5 cut(s) 137, 367, 448, 590, 872
Hin6I GCGC 2 cut(s) 159, 161
HinP1I GCGC 2 cut(s) 159, 161
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HinfI GANTC 7 cut(s) 115, 395, 409, 792, 929, 1106, 1142
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 4 cut(s) 349, 618, 1029, 1135
Hpy188I TCNGA 9 cut(s) 120, 216, 394, 414, 552, 683, 934, 982, 991
Hpy188III TCNNGA 4 cut(s) 280, 364, 608, 815
Hpy8I GTNNAC 4 cut(s) 349, 618, 1029, 1135
Hpy99I CGWCG 2 cut(s) 39, 54
HpyAV CCTTC 4 cut(s) 396, 423, 478, 651
HpyCH4III ACNGT 3 cut(s) 200, 804, 1026
HpyCH4IV ACGT 2 cut(s) 49, 342
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 291, 382, 563, 886, 953, 1181
HpyF3I CTNAG 2 cut(s) 437, 1128
HpySE526I ACGT 2 cut(s) 49, 342
Hsp92II CATG 5 cut(s) 137, 367, 448, 590, 872
HspAI GCGC 2 cut(s) 159, 161
Kzo9I GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
LguI GCTCTTC 2 cut(s) 510, 1182
LmnI GCTCC 3 cut(s) 11, 282, 517
Lsp1109I GCAGC 4 cut(s) 714, 774, 877, 1059
LweI GCATC 4 cut(s) 281, 714, 901, 1077
MaeI CTAG 4 cut(s) 90, 777, 1014, 1041
MaeII ACGT 2 cut(s) 49, 342
MalI GATC 9 cut(s) 360, 495, 537, 546, 576, 606, 753, 789, 1119
MbiI CCGCTC 1 cut(s) 889
MboI GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
MfeI CAATTG 3 cut(s) 270, 353, 1122
MflI RGATCY 3 cut(s) 358, 493, 1117
MhlI GDGCHC 2 cut(s) 351, 848
MluCI AATT 5 cut(s) 270, 353, 641, 662, 1122
MlyI GAGTC 1 cut(s) 1151
MmeI TCCRAC 2 cut(s) 495, 575
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 1176
MseI TTAA 2 cut(s) 78, 155
MspA1I CMGCKG 2 cut(s) 173, 557
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 3 cut(s) 270, 353, 1122
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 7 cut(s) 156, 291, 382, 563, 886, 953, 1181
NdeII GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
NlaIII CATG 5 cut(s) 137, 367, 448, 590, 872
NlaIV GGNNCC 2 cut(s) 894, 1119
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 2 cut(s) 448, 872
PaeI GCATGC 2 cut(s) 448, 872
PagI TCATGA 1 cut(s) 363
PauI GCGCGC 1 cut(s) 159
PciSI GCTCTTC 2 cut(s) 510, 1182
PcsI WCGNNNNNNNCGW 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 1176
PfeI GAWTC 6 cut(s) 115, 395, 409, 792, 929, 1106
PflMI CCANNNNNTGG 1 cut(s) 983
PkrI GCNGC 5 cut(s) 729, 764, 867, 946, 1049
PleI GAGTC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 1150
PshAI GACNNNNGTC 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 894, 1119
PspPI GGNCC 2 cut(s) 1010, 1159
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 1 cut(s) 727
PsuI RGATCY 3 cut(s) 358, 493, 1117
PteI GCGCGC 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 557
RsaI GTAC 3 cut(s) 55, 94, 1028
RsaNI GTAC 3 cut(s) 54, 93, 1027
SapI GCTCTTC 2 cut(s) 510, 1182
SaqAI TTAA 2 cut(s) 78, 155
SatI GCNGC 5 cut(s) 728, 763, 866, 945, 1048
Sau3AI GATC 9 cut(s) 358, 493, 535, 544, 574, 604, 751, 787, 1117
Sau96I GGNCC 2 cut(s) 1010, 1159
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 1 cut(s) 1151
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 2 cut(s) 351, 848
SfaNI GCATC 4 cut(s) 281, 714, 901, 1077
SfcI CTRYAG 1 cut(s) 728
SinI GGWCC 1 cut(s) 1010
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SpeI ACTAGT 1 cut(s) 89
SphI GCATGC 2 cut(s) 448, 872
Sse9I AATT 5 cut(s) 270, 353, 641, 662, 1122
SsiI CCGC 3 cut(s) 171, 889, 945
SspMI CTAG 4 cut(s) 90, 777, 1014, 1041
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 2 cut(s) 18, 903
TaaI ACNGT 3 cut(s) 200, 804, 1026
TaiI ACGT 2 cut(s) 52, 345
TaqI TCGA 4 cut(s) 231, 742, 790, 1170
TasI AATT 5 cut(s) 270, 353, 641, 662, 1122
TatI WGTACW 2 cut(s) 92, 1026
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 6 cut(s) 115, 395, 409, 792, 929, 1106
Tru1I TTAA 2 cut(s) 78, 155
Tru9I TTAA 2 cut(s) 78, 155
TscAI CASTG 2 cut(s) 319, 1078
TseI GCWGC 4 cut(s) 727, 762, 865, 1047
TspDTI ATGAA 3 cut(s) 432, 575, 942
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 2 cut(s) 319, 1078
Van91I CCANNNNNTGG 1 cut(s) 983
VneI GTGCAC 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 1010
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 1 cut(s) 641
XceI RCATGY 2 cut(s) 448, 872
XmnI GAANNNNTTC 1 cut(s) 1176
XspI CTAG 4 cut(s) 90, 777, 1014, 1041
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.