Rw1G030450
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0025822
Physical Location & Seq
Forward (+)
58393720 .. 58396708
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030450.1

Sequence Viewer

Length: 1386 bp
ATGGGGCTTCATACAAGCGTGAAGGCGCATATTAATCTCAGACGCCTCAATTTGGCTGTGGAAACAGACACAGTACATGAGGAGAATGAGACCATCAATACCACTTTCACTGAACCACAAGCTACTGGTTCTGTGGACACAAGGACACCGGATCCACCTGCACCTTCAGTTCTAGAGGCAAGCATGAGCATGGCTTCAACATCATATGGGAATAGCATTTGTGGTGATAACATTCCATGCTTGCCAACATTGAGCTCTGCGGAAAAGAGCATGGCTTCATATGGGACTAGAACCACAGCTGAAAGTCCACCTCCTAACAGAGTGAATTCAATCAGCAAGGTGGCATCGGTCTCAGTACCTTCAACTCCTCAGACAGATGGTGAGATCTTGATGTTCCCCGACCTAAAGAGCTTCTACTTTAGCGAACTGGAAAGAGCAACGAGGAACTTTCATCCTGATAATAAGGTGGATGAAGGTGGTTTTGGTTGTGTCTTCAAGGGTTTGATTGAAAAGAATTCATTAACAGCTGCCAAGCCTGGTTCTGGCATAGTTATTGCTGTGAAGAGGATTCGGCAAGAGAGTTTTGAAGGTCAAATGGACTGGTCGAAAAAAGTCGACTACCTTGGGAAGCTGCATCACCAAAATCTTGTGAAGTTGATTGGCTATTGCTTTGAGGATGACCACCGGCTTCTGGTGTATGAATTTGCACCTCTAGGTAGCTTGCATAACCATCTATTTGGAGGGACTTCGGATGTACAACGACTTTCATGGAACCTTCGGATGAAGATTGCCCTAGGTGCTGCTATGGGTCTATTATATTTTCATAGTTATGGGGCAAAATCAACATATGGGAACTTCAACCCTTCTAAGATCCTTCTTGATTCAAATTACAATGTCAAACTATGTGGTTATGGTTTTGCCAAGGAAAGGCTGGACCGATGGAGACGCCAGGTTATACTTCCTTGGTATGAGGACAGGGTAGGAGTGTCTGCAGGGAATTTGTATGCAGGTACTGCTAAGAATGTATATGCAGCTCCCGAGTGTATAAATCTTCCAAAAGATCAGTTGACCCCTGCAAGTGATATCTATAGTTTTGGGGTTGTTCTGCTGGAACTGTTATCTGGCAGACGAGCTTTTGACTTTGAAAGGCCGCTAAGACAGAGAGATTTAGTTCAGTTGGTCAAGAAATTTCAATCAATGAGCAAATATGAAGTTGACCAAATTTTTGATGCTCATATTGAAGGCCAGTACCCTGAGCGTGGAGCTAAAAAAGTATTGGCCCTTGTAAGTTTATGCCTGCGAGAAGCCGAGTTCAGGATAAGCATGAATGTGGTGGTAACAGAGTTGAAGCGGTGGTATGCCAATTCAGGAGAAGGTCTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

461

Amino Acids

51.4

Weight (kDa)

8.04

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 153 - 448 3.8e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 153 - 436 1.7e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025822)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_4G00289360
rosa_wichuraiana Rw1G030450 Rw1G030500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 2 cut(s) 166, 998
AccI GTMKAC 1 cut(s) 615
AciI CCGC 3 cut(s) 260, 1151, 1351
AclWI GGATC 3 cut(s) 146, 159, 865
AcsI RAATTY 6 cut(s) 325, 514, 701, 997, 1187, 1221
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 2 cut(s) 43, 946
AfaI GTAC 5 cut(s) 75, 357, 756, 1012, 1250
AfiI CCNNNNNNNGG 6 cut(s) 52, 542, 691, 927, 1259, 1314
AjnI CCWGG 2 cut(s) 535, 948
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 4 cut(s) 83, 355, 937, 1382
AlwI GGATC 3 cut(s) 146, 159, 865
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 1037
AoxI GGCC 3 cut(s) 1148, 1243, 1278
ApeKI GCWGC 4 cut(s) 527, 631, 800, 1031
ApoI RAATTY 6 cut(s) 325, 514, 701, 997, 1187, 1221
ArsI GACNNNNNNTTYG 2 cut(s) 1208, 1240
AseI ATTAAT 1 cut(s) 33
AspA2I CCTAGG 1 cut(s) 793
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 2 cut(s) 934, 1279
AsuHPI GGTGA 3 cut(s) 236, 392, 629
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 1 cut(s) 934
AvrII CCTAGG 1 cut(s) 793
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 4 cut(s) 514, 618, 787, 1043
BccI CCATC 4 cut(s) 101, 371, 738, 933
BciT130I CCWGG 2 cut(s) 537, 950
BcoDI GTCTC 4 cut(s) 83, 355, 937, 1382
BfaI CTAG 4 cut(s) 173, 288, 713, 794
BfmI CTRYAG 2 cut(s) 990, 1087
BfuAI ACCTGC 2 cut(s) 166, 998
BglII AGATCT 1 cut(s) 384
BisI GCNGC 5 cut(s) 528, 632, 801, 1032, 1151
BlnI CCTAGG 1 cut(s) 793
BlsI GCNGC 5 cut(s) 529, 633, 802, 1033, 1152
Bme1390I CCNGG 2 cut(s) 537, 950
Bme18I GGWCC 1 cut(s) 934
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 2 cut(s) 934, 1279
BmiI GGNNCC 2 cut(s) 153, 773
BmrFI CCNGG 2 cut(s) 537, 950
BmsI GCATC 3 cut(s) 353, 643, 1219
BpiI GAAGAC 1 cut(s) 484
BplI GAGNNNNNCTC 1 cut(s) 1362
Bpu10I CCTNAGC 1 cut(s) 1254
BsaHI GRCGYC 2 cut(s) 43, 946
BsaI GGTCTC 3 cut(s) 83, 355, 1382
BsaJI CCNNGG 4 cut(s) 622, 793, 921, 962
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 542, 691, 927, 1259, 1314
Bse118I RCCGGY 1 cut(s) 684
Bse1I ACTGG 4 cut(s) 130, 432, 605, 1246
BseBI CCWGG 2 cut(s) 537, 950
BseDI CCNNGG 4 cut(s) 622, 793, 921, 962
BseGI GGATG 5 cut(s) 451, 475, 682, 757, 786
BseLI CCNNNNNNNGG 6 cut(s) 52, 542, 691, 927, 1259, 1314
BseMII CTCAG 4 cut(s) 52, 366, 383, 1245
BseNI ACTGG 4 cut(s) 130, 432, 605, 1246
BseRI GAGGAG 2 cut(s) 95, 357
BseXI GCAGC 4 cut(s) 514, 618, 787, 1043
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 3 cut(s) 1150, 1245, 1280
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 2 cut(s) 149, 685
BslFI GGGAC 2 cut(s) 298, 757
BslI CCNNNNNNNGG 6 cut(s) 52, 542, 691, 927, 1259, 1314
BsmAI GTCTC 4 cut(s) 83, 355, 937, 1382
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 2 cut(s) 298, 757
BsnI GGCC 3 cut(s) 1150, 1245, 1280
Bso31I GGTCTC 3 cut(s) 83, 355, 1382
BsoBI CYCGRG 1 cut(s) 1037
Bsp1286I GDGCHC 1 cut(s) 257
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 4 cut(s) 151, 384, 870, 1060
BspACI CCGC 3 cut(s) 260, 1151, 1351
BspANI GGCC 3 cut(s) 1150, 1245, 1280
BspCNI CTCAG 4 cut(s) 51, 365, 382, 1246
BspLI GGNNCC 2 cut(s) 153, 773
BspMAI CTGCAG 1 cut(s) 994
BspMI ACCTGC 2 cut(s) 166, 998
BspPI GGATC 3 cut(s) 146, 159, 865
BspTNI GGTCTC 3 cut(s) 83, 355, 1382
BsrFI RCCGGY 1 cut(s) 684
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 4 cut(s) 130, 432, 605, 1246
BssAI RCCGGY 1 cut(s) 684
BssECI CCNNGG 4 cut(s) 622, 793, 921, 962
BssMI GATC 4 cut(s) 151, 384, 870, 1060
BssNI GRCGYC 2 cut(s) 43, 946
BssT1I CCWWGG 4 cut(s) 622, 793, 921, 962
Bst2UI CCWGG 2 cut(s) 537, 950
Bst4CI ACNGT 2 cut(s) 73, 1116
Bst6I CTCTTC 1 cut(s) 557
BstACI GRCGYC 2 cut(s) 43, 946
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 754
BstC8I GCNNGC 4 cut(s) 181, 242, 722, 1298
BstDEI CTNAG 7 cut(s) 38, 352, 369, 867, 1017, 1154, 1254
BstF5I GGATG 5 cut(s) 451, 475, 682, 757, 786
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 4 cut(s) 154, 387, 873, 1063
BstMAI GTCTC 4 cut(s) 83, 355, 937, 1382
BstMBI GATC 4 cut(s) 151, 384, 870, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 797, 1013
BstNI CCWGG 2 cut(s) 537, 950
BstSCI CCNGG 2 cut(s) 535, 948
BstSFI CTRYAG 2 cut(s) 990, 1087
BstV1I GCAGC 4 cut(s) 514, 618, 787, 1043
BstV2I GAAGAC 1 cut(s) 484
BstX2I RGATCY 3 cut(s) 151, 384, 870
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 3 cut(s) 151, 384, 870
BsuRI GGCC 3 cut(s) 1150, 1245, 1280
BtsCI GGATG 5 cut(s) 451, 475, 682, 757, 786
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 2 cut(s) 166, 998
Cac8I GCNNGC 4 cut(s) 181, 242, 722, 1298
CaiI CAGNNNCTG 1 cut(s) 1013
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 934, 1279
CseI GACGC 2 cut(s) 51, 954
Csp6I GTAC 5 cut(s) 74, 356, 755, 1011, 1249
CspCI CAANNNNNGTGG 2 cut(s) 886, 921
CviAII CATG 7 cut(s) 77, 184, 190, 237, 271, 768, 1324
CviQI GTAC 5 cut(s) 74, 356, 755, 1011, 1249
DdeI CTNAG 7 cut(s) 38, 352, 369, 867, 1017, 1154, 1254
DpnI GATC 4 cut(s) 153, 386, 872, 1062
DpnII GATC 4 cut(s) 151, 384, 870, 1060
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 255
Eco130I CCWWGG 4 cut(s) 622, 793, 921, 962
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 3 cut(s) 83, 355, 1382
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 1 cut(s) 934
Eco53kI GAGCTC 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 1037
EcoICRI GAGCTC 1 cut(s) 255
EcoRI GAATTC 2 cut(s) 325, 514
EcoRII CCWGG 2 cut(s) 535, 948
EcoRV GATATC 1 cut(s) 1084
EcoT14I CCWWGG 4 cut(s) 622, 793, 921, 962
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 4 cut(s) 622, 793, 921, 962
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 7 cut(s) 80, 187, 193, 240, 274, 771, 1327
FaqI GGGAC 2 cut(s) 298, 757
FatI CATG 7 cut(s) 76, 183, 189, 236, 270, 767, 1323
FauNDI CATATG 3 cut(s) 205, 280, 847
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 5 cut(s) 528, 632, 801, 1032, 1151
FokI GGATG 5 cut(s) 438, 482, 689, 764, 793
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 5 cut(s) 528, 632, 801, 1032, 1151
FspBI CTAG 4 cut(s) 173, 288, 713, 794
GlaI GCGC 1 cut(s) 27
GluI GCNGC 5 cut(s) 528, 632, 801, 1032, 1151
HaeIII GGCC 3 cut(s) 1150, 1245, 1280
HapII CCGG 2 cut(s) 149, 685
HgaI GACGC 2 cut(s) 51, 954
HhaI GCGC 1 cut(s) 28
Hin1I GRCGYC 2 cut(s) 43, 946
Hin1II CATG 7 cut(s) 80, 187, 193, 240, 274, 771, 1327
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 3 cut(s) 616, 1068, 1216
HindII GTYRAC 3 cut(s) 616, 1068, 1216
HinfI GANTC 2 cut(s) 568, 881
HpaII CCGG 2 cut(s) 149, 685
HphI GGTGA 3 cut(s) 236, 392, 629
Hpy166II GTNNAC 5 cut(s) 136, 308, 616, 1068, 1216
Hpy188I TCNGA 4 cut(s) 41, 372, 751, 780
Hpy188III TCNNGA 8 cut(s) 173, 388, 455, 878, 1037, 1183, 1315, 1368
Hpy8I GTNNAC 5 cut(s) 136, 308, 616, 1068, 1216
HpyCH4III ACNGT 2 cut(s) 73, 1116
HpyCH4V TGCA 8 cut(s) 161, 634, 707, 724, 992, 1007, 1031, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 797, 1013
HpyF3I CTNAG 7 cut(s) 38, 352, 369, 867, 1017, 1154, 1254
Hsp92I GRCGYC 2 cut(s) 43, 946
Hsp92II CATG 7 cut(s) 80, 187, 193, 240, 274, 771, 1327
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 4 cut(s) 151, 384, 870, 1060
LmnI GCTCC 2 cut(s) 1039, 1262
Lsp1109I GCAGC 4 cut(s) 514, 618, 787, 1043
LweI GCATC 3 cut(s) 353, 643, 1219
MaeI CTAG 4 cut(s) 173, 288, 713, 794
MaeIII GTNAC 1 cut(s) 1336
MalI GATC 4 cut(s) 153, 386, 872, 1062
MboI GATC 4 cut(s) 151, 384, 870, 1060
MboII GAAGA 4 cut(s) 484, 574, 796, 1043
MflI RGATCY 3 cut(s) 151, 384, 870
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 9 cut(s) 49, 325, 514, 701, 886, 997, 1187, 1221, 1363
MseI TTAA 2 cut(s) 33, 521
MslI CAYNNNNRTG 3 cut(s) 188, 828, 1328
MspA1I CMGCKG 2 cut(s) 299, 527
MspI CCGG 2 cut(s) 149, 685
MspR9I CCNGG 2 cut(s) 537, 950
MvaI CCWGG 2 cut(s) 537, 950
MwoI GCNNNNNNNGC 2 cut(s) 797, 1013
NdeI CATATG 3 cut(s) 205, 280, 847
NdeII GATC 4 cut(s) 151, 384, 870, 1060
NlaIII CATG 7 cut(s) 80, 187, 193, 240, 274, 771, 1327
NlaIV GGNNCC 2 cut(s) 153, 773
NmeAIII GCCGAG 1 cut(s) 1333
PaqCI CACCTGC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 568, 881
PkrI GCNGC 5 cut(s) 529, 633, 802, 1033, 1152
PshBI ATTAAT 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 535, 948
PspGI CCWGG 2 cut(s) 535, 948
PspN4I GGNNCC 2 cut(s) 153, 773
PspPI GGNCC 2 cut(s) 934, 1279
PstI CTGCAG 1 cut(s) 994
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 3 cut(s) 151, 384, 870
PvuII CAGCTG 2 cut(s) 299, 527
RsaI GTAC 5 cut(s) 75, 357, 756, 1012, 1250
RsaNI GTAC 5 cut(s) 74, 356, 755, 1011, 1249
RseI CAYNNNNRTG 3 cut(s) 188, 828, 1328
SacI GAGCTC 1 cut(s) 257
SalI GTCGAC 1 cut(s) 614
SaqAI TTAA 2 cut(s) 33, 521
SatI GCNGC 5 cut(s) 528, 632, 801, 1032, 1151
Sau3AI GATC 4 cut(s) 151, 384, 870, 1060
Sau96I GGNCC 2 cut(s) 934, 1279
ScrFI CCNGG 2 cut(s) 537, 950
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 3 cut(s) 353, 643, 1219
SfcI CTRYAG 2 cut(s) 990, 1087
SinI GGWCC 1 cut(s) 934
SmiMI CAYNNNNRTG 3 cut(s) 188, 828, 1328
Sse9I AATT 9 cut(s) 49, 325, 514, 701, 886, 997, 1187, 1221, 1363
SsiI CCGC 3 cut(s) 260, 1151, 1351
SspMI CTAG 4 cut(s) 173, 288, 713, 794
SstI GAGCTC 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 535, 948
StyI CCWWGG 4 cut(s) 622, 793, 921, 962
TaaI ACNGT 2 cut(s) 73, 1116
TaqI TCGA 2 cut(s) 605, 615
TaqII GACCGA 2 cut(s) 337, 951
TasI AATT 9 cut(s) 49, 325, 514, 701, 886, 997, 1187, 1221, 1363
TatI WGTACW 2 cut(s) 73, 754
TauI GCSGC 1 cut(s) 1153
TfiI GAWTC 2 cut(s) 568, 881
Tru1I TTAA 2 cut(s) 33, 521
Tru9I TTAA 2 cut(s) 33, 521
TscAI CASTG 1 cut(s) 115
TseI GCWGC 4 cut(s) 527, 631, 800, 1031
TspRI CASTG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 934
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 6 cut(s) 325, 514, 701, 997, 1187, 1221
XbaI TCTAGA 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 928
XmaJI CCTAGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 615
XspI CTAG 4 cut(s) 173, 288, 713, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.