Rw2G054120

beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018469
Physical Location & Seq
Forward (+)
81808094 .. 81814289
6196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054120.1

Sequence Viewer

Length: 1707 bp
ATGAGGCCGTCGATTTGCTCAATGGAACAGTACTGTTGGTTCTGTGTTCTTCTTGGTTTAGAAACCAGTGACTACTTACCCTTATCTCATACTCTCAGAGATTTCAAAGATTCCAGCAAAACCCAGGTTTCTGTGCAGTTCGGTAGGGGGTGCTGGTTCTGCAGATTTTATATGATGATGTCCTGTTTGACAGCAAGAACAATGGAGGCTTGTTTCCGGGGATGCAGCCACGTCTTTGAGCTTGTGGCGGGTACAGGTGCTCTCGATGACGCAAAACTTCCTAACCTCAATAAGGCTCAGTCGACTAGTACTGGAAGCGAAACTATTAGCAGGAAAGACTTCCCCGCAGATTTCAAATTTGGTTGCGCCACTTCTGCTTTGCAGACGGAAGGATCCGGAACTGAAGGAGGGAGAGGACCAGCGATATGGGATAAATACATACAAGATGGCACTCCAGGCATAAAAATTGCCGTTGATTCCTACCACCGGTATAAGGAGGACGTGCAACTGCTCAAGGCAATAGGAGTGGATACTTACAGATTCTCCATGTCTTGGTCAAGAATTCTCCCTGGGGGAACCATAGACGCTGGAATAAACCAGGAGGGTATCGATTTCTATAACAACTTTATCAACGAACTAATTTCCAATGGGATAACTCCATTTGTGACACTGTTCCACTTCGACTTGCCCACAGCACTGAGTACTAAATACAACGGTTTTTTAAGCATTGATATCGTGGATGATTTTAAAGCGTATGCGGATTTATGTTTCAAAACTTTTGGCGATCGAGTGAAGTATTGGGCTACTATCAACGAGCCACAAGTGTGGGGGGAATACGGCTTTACTAACTCAGATCCAAACGCAAACGCTGCCACTGATCCTTTCCTCTCTACACATCACGTTTTATTGGCTCACGCGGCTGCCGCCAAACTCTACAAAAACACATACCAGGCTTCACAAGGAGGAGAGATTGGAATTCCCCTGGTTGTTGAATGGTTTGAGCCATTTGAGGACACCATGCCAGATAAAGCTGCGGCCAGTAGAGCATTTGATTTCTTAGTCGGATGGCTTGTGGAGCCACTTGTATATGGTGATTACCCGTTCATTATGAAAGCTCTAGTAAAGGATGGACTTCCAGAATTCACTGATGAGCAGAAAGAGTTGGTGAAAGGGTCATTCGATTATATTGGGGTGAACTACTATACTTCCCGATTCGCGGCTCCAATAGCAATAGTCGACGGTGAAGAAATTACCAGCTTTGATCAGTACCAGCACGTTAACATCACAGTGGATGGACCAGATGGTGAACCCATAGGCCCTGCAACACCAGGGAGCAGTGAGATCTACGTTTACCCAGAAGGTCTGAAAAAGGCATTGATTTTACTGAAGGAATACGGCAATCCCAAATTCTACATTACTGAGAATGGGTATCCAGATAAACGAGATGACACCATTCCACTTTCAACTGCGTTGATTGACGATGCTCGAATTCATCATATAAGGGATCATCTCACAGCCATTAAAGAGGCCATGAACACCGGAACAAATGTGAAGGGTTACCTGATGTGGGCGCTGTTGGACTGCATGGAGCTGGGATCGGGCTACCAAGTTCGATTTGGTCTGAATTACACAGATTACCTTAACAACTTGAACCGAACTGCCAAGAAATCTGCCGGCTGGCTCACAACTTTCTTGGCTTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

568

Amino Acids

63.36

Weight (kDa)

5.02

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 111 - 566 1.9e-130 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018469)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g53000 FvH4_6g53000
rosa_chinensis RchiOBHm_Chr2g0174751
rosa_laevigata RLG00000022292
rosa_roxburghii Rroxscaffold_2G00077770
rosa_rugosa Rorug02G0579200
rosa_samantha Rh1BG121400 Rh2CG634600 Rh2DG684900
rosa_wichuraiana Rw2G054120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 552
AccI GTMKAC 2 cut(s) 302, 1236
AccII CGCG 2 cut(s) 917, 1217
AccIII TCCGGA 1 cut(s) 395
AciI CCGC 7 cut(s) 248, 345, 758, 917, 924, 1034, 1217
AclWI GGATC 6 cut(s) 387, 400, 848, 872, 1512, 1603
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 6 cut(s) 356, 561, 975, 1139, 1406, 1488
AcuI CTGAAG 2 cut(s) 423, 1406
AfaI GTAC 5 cut(s) 32, 253, 310, 703, 1268
AfiI CCNNNNNNNGG 6 cut(s) 292, 486, 493, 552, 1216, 1567
AgeI ACCGGT 1 cut(s) 486
AgsI TTSAA 6 cut(s) 106, 355, 772, 992, 1464, 1651
AhlI ACTAGT 1 cut(s) 305
AjiI CACGTC 2 cut(s) 232, 502
AjnI CCWGG 7 cut(s) 123, 454, 568, 597, 948, 981, 1327
AleI CACNNNNGTG 1 cut(s) 823
AluBI AGCT 5 cut(s) 241, 1031, 1115, 1257, 1591
AluI AGCT 5 cut(s) 241, 1031, 1115, 1257, 1591
Alw21I GWGCWC 1 cut(s) 262
AlwI GGATC 6 cut(s) 387, 400, 848, 872, 1512, 1603
Aor13HI TCCGGA 1 cut(s) 395
AoxI GGCC 4 cut(s) 5, 1035, 1315, 1527
ApeKI GCWGC 4 cut(s) 225, 869, 920, 1031
ApoI RAATTY 6 cut(s) 356, 561, 975, 1139, 1406, 1488
AsiGI ACCGGT 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 2 cut(s) 368, 1573
AspS9I GGNCC 3 cut(s) 416, 1295, 1316
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 5 cut(s) 1103, 1177, 1204, 1253, 1316
AvaII GGWCC 2 cut(s) 416, 1295
BamHI GGATCC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 262
BbvI GCAGC 4 cut(s) 237, 856, 907, 1018
BccI CCATC 5 cut(s) 440, 1059, 1121, 1286, 1295
BceAI ACGGC 3 cut(s) 455, 853, 1411
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 7 cut(s) 125, 456, 570, 599, 950, 983, 1329
BciVI GTATCC 2 cut(s) 523, 1440
BclI TGATCA 1 cut(s) 1261
BcnI CCSGG 1 cut(s) 218
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 2 cut(s) 306, 1118
BfmI CTRYAG 1 cut(s) 160
BfoI RGCGCY 1 cut(s) 1574
BfuI GTATCC 2 cut(s) 523, 1440
BglII AGATCT 1 cut(s) 1341
BisI GCNGC 8 cut(s) 226, 870, 918, 921, 924, 1032, 1035, 1218
BlsI GCNGC 8 cut(s) 227, 871, 919, 922, 925, 1033, 1036, 1219
BmcAI AGTACT 3 cut(s) 32, 310, 703
Bme1390I CCNGG 8 cut(s) 125, 218, 456, 570, 599, 950, 983, 1329
Bme18I GGWCC 2 cut(s) 416, 1295
BmgBI CACGTC 2 cut(s) 232, 502
BmgT120I GGNCC 3 cut(s) 416, 1295, 1316
BmiI GGNNCC 4 cut(s) 394, 577, 1077, 1221
BmrFI CCNGG 8 cut(s) 125, 218, 456, 570, 599, 950, 983, 1329
BmsI GCATC 2 cut(s) 212, 1471
BpmI CTGGAG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 609
BsaJI CCNNGG 6 cut(s) 123, 217, 568, 569, 981, 1328
BsaWI WCCGGW 3 cut(s) 395, 486, 1538
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 6 cut(s) 292, 486, 493, 552, 1216, 1567
Bse118I RCCGGY 2 cut(s) 486, 1673
Bse1I ACTGG 3 cut(s) 66, 316, 1038
BseAI TCCGGA 1 cut(s) 395
BseBI CCWGG 7 cut(s) 125, 456, 570, 599, 950, 983, 1329
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 6 cut(s) 123, 217, 568, 569, 981, 1328
BseGI GGATG 5 cut(s) 227, 745, 1070, 1132, 1297
BseLI CCNNNNNNNGG 6 cut(s) 292, 486, 493, 552, 1216, 1567
BseMII CTCAG 5 cut(s) 109, 311, 689, 864, 1410
BseNI ACTGG 3 cut(s) 66, 316, 1038
BseRI GAGGAG 1 cut(s) 978
BseXI GCAGC 4 cut(s) 237, 856, 907, 1018
BseYI CCCAGC 1 cut(s) 1591
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 2 cut(s) 917, 1217
Bsh1285I CGRYCG 1 cut(s) 787
BshFI GGCC 4 cut(s) 7, 1037, 1317, 1529
BshTI ACCGGT 1 cut(s) 486
BshVI ATCGAT 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 787
BsiHKAI GWGCWC 1 cut(s) 262
BsiSI CCGG 5 cut(s) 217, 396, 487, 1539, 1674
BslI CCNNNNNNNGG 6 cut(s) 292, 486, 493, 552, 1216, 1567
BsnI GGCC 4 cut(s) 7, 1037, 1317, 1529
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 1 cut(s) 395
Bsp143I GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
BspACI CCGC 7 cut(s) 248, 345, 758, 917, 924, 1034, 1217
BspANI GGCC 4 cut(s) 7, 1037, 1317, 1529
BspCNI CTCAG 5 cut(s) 108, 310, 690, 863, 1411
BspDI ATCGAT 1 cut(s) 609
BspEI TCCGGA 1 cut(s) 395
BspFNI CGCG 2 cut(s) 917, 1217
BspLI GGNNCC 4 cut(s) 394, 577, 1077, 1221
BspMAI CTGCAG 1 cut(s) 164
BspPI GGATC 6 cut(s) 387, 400, 848, 872, 1512, 1603
BsrFI RCCGGY 2 cut(s) 486, 1673
BsrI ACTGG 3 cut(s) 66, 316, 1038
BssAI RCCGGY 2 cut(s) 486, 1673
BssECI CCNNGG 6 cut(s) 123, 217, 568, 569, 981, 1328
BssMI GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
Bst2UI CCWGG 7 cut(s) 125, 456, 570, 599, 950, 983, 1329
Bst4CI ACNGT 6 cut(s) 30, 35, 672, 716, 1241, 1288
BstAPI GCANNNNNTGC 1 cut(s) 869
BstC8I GCNNGC 2 cut(s) 1675, 1679
BstDEI CTNAG 6 cut(s) 95, 297, 698, 850, 1057, 1419
BstEII GGTNACC 1 cut(s) 1556
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 5 cut(s) 227, 745, 1070, 1132, 1297
BstFNI CGCG 2 cut(s) 917, 1217
BstH2I RGCGCY 1 cut(s) 1574
BstHHI GCGC 2 cut(s) 368, 1573
BstKTI GATC 8 cut(s) 395, 787, 856, 880, 1264, 1344, 1507, 1598
BstMBI GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
BstMCI CGRYCG 1 cut(s) 787
BstMWI GCNNNNNNNGC 9 cut(s) 159, 374, 456, 869, 917, 923, 1043, 1075, 1226
BstNI CCWGG 7 cut(s) 125, 456, 570, 599, 950, 983, 1329
BstPI GGTNACC 1 cut(s) 1556
BstSCI CCNGG 8 cut(s) 123, 216, 454, 568, 597, 948, 981, 1327
BstSFI CTRYAG 1 cut(s) 160
BstUI CGCG 2 cut(s) 917, 1217
BstV1I GCAGC 4 cut(s) 237, 856, 907, 1018
BstX2I RGATCY 3 cut(s) 392, 853, 1341
BstXI CCANNNNNNTGG 2 cut(s) 426, 825
BstYI RGATCY 3 cut(s) 392, 853, 1341
Bsu15I ATCGAT 1 cut(s) 609
BsuI GTATCC 2 cut(s) 523, 1440
BsuRI GGCC 4 cut(s) 7, 1037, 1317, 1529
BsuTUI ATCGAT 1 cut(s) 609
BtrI CACGTC 2 cut(s) 232, 502
BtsCI GGATG 5 cut(s) 227, 745, 1070, 1132, 1297
BtsI GCAGTG 1 cut(s) 1342
BtsIMutI CAGTG 7 cut(s) 73, 668, 695, 873, 1143, 1293, 1342
Cac8I GCNNGC 2 cut(s) 1675, 1679
CfoI GCGC 2 cut(s) 368, 1573
Cfr10I RCCGGY 2 cut(s) 486, 1673
Cfr13I GGNCC 3 cut(s) 416, 1295, 1316
ClaI ATCGAT 1 cut(s) 609
CseI GACGC 2 cut(s) 278, 593
Csp6I GTAC 5 cut(s) 31, 252, 309, 702, 1267
CspAI ACCGGT 1 cut(s) 486
CspCI CAANNNNNGTGG 2 cut(s) 507, 542
CviAII CATG 4 cut(s) 547, 1018, 1531, 1585
CviQI GTAC 5 cut(s) 31, 252, 309, 702, 1267
DdeI CTNAG 6 cut(s) 95, 297, 698, 850, 1057, 1419
DpnI GATC 8 cut(s) 394, 786, 855, 879, 1263, 1343, 1506, 1597
DpnII GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
DraI TTTAAA 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 1035
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 416, 1295
Eco57I CTGAAG 2 cut(s) 423, 1406
Eco91I GGTNACC 1 cut(s) 1556
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 1316
EcoO65I GGTNACC 1 cut(s) 1556
EcoRI GAATTC 4 cut(s) 561, 975, 1139, 1488
EcoRII CCWGG 7 cut(s) 123, 454, 568, 597, 948, 981, 1327
EcoRV GATATC 1 cut(s) 733
FaeI CATG 4 cut(s) 550, 1021, 1534, 1588
FatI CATG 4 cut(s) 546, 1017, 1530, 1584
FauI CCCGC 2 cut(s) 241, 352
FbaI TGATCA 1 cut(s) 1261
FblI GTMKAC 2 cut(s) 302, 1236
Fnu4HI GCNGC 8 cut(s) 226, 870, 918, 921, 924, 1032, 1035, 1218
FokI GGATG 5 cut(s) 234, 752, 1077, 1139, 1304
Fsp4HI GCNGC 8 cut(s) 226, 870, 918, 921, 924, 1032, 1035, 1218
FspBI CTAG 2 cut(s) 306, 1118
GlaI GCGC 2 cut(s) 367, 1572
GluI GCNGC 8 cut(s) 226, 870, 918, 921, 924, 1032, 1035, 1218
GsaI CCCAGC 1 cut(s) 1595
GsuI CTGGAG 1 cut(s) 438
HaeII RGCGCY 1 cut(s) 1574
HaeIII GGCC 4 cut(s) 7, 1037, 1317, 1529
HapII CCGG 5 cut(s) 217, 396, 487, 1539, 1674
HgaI GACGC 2 cut(s) 278, 593
HhaI GCGC 2 cut(s) 368, 1573
Hin1II CATG 4 cut(s) 550, 1021, 1534, 1588
Hin6I GCGC 2 cut(s) 366, 1571
HinP1I GCGC 2 cut(s) 366, 1571
HincII GTYRAC 3 cut(s) 303, 1237, 1279
HindII GTYRAC 3 cut(s) 303, 1237, 1279
HinfI GANTC 4 cut(s) 110, 476, 540, 1212
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 5 cut(s) 217, 396, 487, 1539, 1674
HphI GGTGA 5 cut(s) 1103, 1177, 1204, 1253, 1316
Hpy166II GTNNAC 6 cut(s) 303, 1195, 1237, 1279, 1307, 1351
Hpy188I TCNGA 5 cut(s) 98, 853, 1064, 1365, 1623
Hpy188III TCNNGA 6 cut(s) 263, 396, 558, 1136, 1209, 1433
Hpy8I GTNNAC 6 cut(s) 303, 1195, 1237, 1279, 1307, 1351
Hpy99I CGWCG 2 cut(s) 13, 1241
HpyAV CCTTC 5 cut(s) 383, 398, 1352, 1381, 1546
HpyCH4III ACNGT 6 cut(s) 30, 35, 672, 716, 1241, 1288
HpyCH4IV ACGT 5 cut(s) 231, 501, 900, 1275, 1347
HpyCH4V TGCA 7 cut(s) 136, 162, 225, 382, 505, 1322, 1584
HpyF10VI GCNNNNNNNGC 9 cut(s) 159, 374, 456, 869, 917, 923, 1043, 1075, 1226
HpyF3I CTNAG 6 cut(s) 95, 297, 698, 850, 1057, 1419
HpySE526I ACGT 5 cut(s) 231, 501, 900, 1275, 1347
Hsp92II CATG 4 cut(s) 550, 1021, 1534, 1588
HspAI GCGC 2 cut(s) 366, 1571
Kpn2I TCCGGA 1 cut(s) 395
KroI GCCGGC 1 cut(s) 1673
KroNI GCCGGC 1 cut(s) 1675
Ksp22I TGATCA 1 cut(s) 1261
KspAI GTTAAC 1 cut(s) 1279
Kzo9I GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
LmnI GCTCC 4 cut(s) 1075, 1225, 1332, 1588
Lsp1109I GCAGC 4 cut(s) 237, 856, 907, 1018
LweI GCATC 2 cut(s) 212, 1471
MaeI CTAG 2 cut(s) 306, 1118
MaeII ACGT 5 cut(s) 231, 501, 900, 1275, 1347
MaeIII GTNAC 3 cut(s) 68, 664, 1556
MalI GATC 8 cut(s) 394, 786, 855, 879, 1263, 1343, 1506, 1597
MboI GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
MboII GAAGA 2 cut(s) 41, 1256
MflI RGATCY 3 cut(s) 392, 853, 1341
MhlI GDGCHC 1 cut(s) 262
MmeI TCCRAC 2 cut(s) 1042, 1557
MroI TCCGGA 1 cut(s) 395
MroNI GCCGGC 1 cut(s) 1673
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 5 cut(s) 722, 747, 1278, 1521, 1641
MslI CAYNNNNRTG 2 cut(s) 823, 1286
MspI CCGG 5 cut(s) 217, 396, 487, 1539, 1674
MspR9I CCNGG 8 cut(s) 125, 218, 456, 570, 599, 950, 983, 1329
MvaI CCWGG 7 cut(s) 125, 456, 570, 599, 950, 983, 1329
MvnI CGCG 2 cut(s) 917, 1217
MwoI GCNNNNNNNGC 9 cut(s) 159, 374, 456, 869, 917, 923, 1043, 1075, 1226
NaeI GCCGGC 1 cut(s) 1675
NciI CCSGG 1 cut(s) 218
NdeII GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
NgoMIV GCCGGC 1 cut(s) 1673
NlaIII CATG 4 cut(s) 550, 1021, 1534, 1588
NlaIV GGNNCC 4 cut(s) 394, 577, 1077, 1221
NmuCI GTSAC 2 cut(s) 68, 664
OliI CACNNNNGTG 1 cut(s) 823
PasI CCCWGGG 1 cut(s) 569
PdiI GCCGGC 1 cut(s) 1675
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 4 cut(s) 110, 476, 540, 1212
PflMI CCANNNNNTGG 1 cut(s) 552
PinAI ACCGGT 1 cut(s) 486
PkrI GCNGC 8 cut(s) 227, 871, 919, 922, 925, 1033, 1036, 1219
Ple19I CGATCG 1 cut(s) 787
Psp6I CCWGG 7 cut(s) 123, 454, 568, 597, 948, 981, 1327
PspEI GGTNACC 1 cut(s) 1556
PspFI CCCAGC 1 cut(s) 1591
PspGI CCWGG 7 cut(s) 123, 454, 568, 597, 948, 981, 1327
PspN4I GGNNCC 4 cut(s) 394, 577, 1077, 1221
PspPI GGNCC 3 cut(s) 416, 1295, 1316
PsrI GAACNNNNNNTAC 2 cut(s) 23, 55
PstI CTGCAG 1 cut(s) 164
PsuI RGATCY 3 cut(s) 392, 853, 1341
PvuI CGATCG 1 cut(s) 787
RsaI GTAC 5 cut(s) 32, 253, 310, 703, 1268
RsaNI GTAC 5 cut(s) 31, 252, 309, 702, 1267
RseI CAYNNNNRTG 2 cut(s) 823, 1286
SalI GTCGAC 2 cut(s) 301, 1235
SaqAI TTAA 5 cut(s) 722, 747, 1278, 1521, 1641
SatI GCNGC 8 cut(s) 226, 870, 918, 921, 924, 1032, 1035, 1218
Sau3AI GATC 8 cut(s) 392, 784, 853, 877, 1261, 1341, 1504, 1595
Sau96I GGNCC 3 cut(s) 416, 1295, 1316
ScaI AGTACT 3 cut(s) 32, 310, 703
ScrFI CCNGG 8 cut(s) 125, 218, 456, 570, 599, 950, 983, 1329
SduI GDGCHC 1 cut(s) 262
SfaNI GCATC 2 cut(s) 212, 1471
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 2 cut(s) 416, 1295
SmiMI CAYNNNNRTG 2 cut(s) 823, 1286
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SpeI ACTAGT 1 cut(s) 305
SsiI CCGC 7 cut(s) 248, 345, 758, 917, 924, 1034, 1217
SspMI CTAG 2 cut(s) 306, 1118
StyD4I CCNGG 8 cut(s) 123, 216, 454, 568, 597, 948, 981, 1327
TaaI ACNGT 6 cut(s) 30, 35, 672, 716, 1241, 1288
TaiI ACGT 5 cut(s) 234, 504, 903, 1278, 1350
TatI WGTACW 3 cut(s) 30, 308, 701
TauI GCSGC 4 cut(s) 920, 926, 1037, 1220
TfiI GAWTC 4 cut(s) 110, 476, 540, 1212
Tru1I TTAA 5 cut(s) 722, 747, 1278, 1521, 1641
Tru9I TTAA 5 cut(s) 722, 747, 1278, 1521, 1641
TscAI CASTG 7 cut(s) 73, 675, 702, 880, 1150, 1293, 1342
TseFI GTSAC 2 cut(s) 68, 664
TseI GCWGC 4 cut(s) 225, 869, 920, 1031
Tsp45I GTSAC 2 cut(s) 68, 664
TspDTI ATGAA 4 cut(s) 1093, 1124, 1481, 1547
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 7 cut(s) 73, 675, 702, 880, 1150, 1293, 1342
Van91I CCANNNNNTGG 1 cut(s) 552
VpaK11BI GGWCC 2 cut(s) 416, 1295
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 6 cut(s) 356, 561, 975, 1139, 1406, 1488
XcmI CCANNNNNNNNNTGG 1 cut(s) 1613
XmiI GTMKAC 2 cut(s) 302, 1236
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 2 cut(s) 306, 1118
ZrmI AGTACT 3 cut(s) 32, 310, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.