Rw4G011290

Leucine rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017559
Physical Location & Seq
Forward (+)
26795691 .. 26796911
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011290.1

Sequence Viewer

Length: 1221 bp
ATGGGCAAGCTGATGCCTCTCTTTTGTCTAGTGTATTTCACAGTATGTTCCTCTCCATTGTTTACTAGCTCACTAACACTCCATTCCGATATTCAAGTTCTTCATGTCATGAAGCGTTGCATGGATCTATACTCCATCCCCCCAAATTCCTATCTGAGTAGCTGGGACTTCATACACGATCCATGTGAAACTACAGGCGGGCAGTTTCTCGGAGTCTTATGCAGTTTCCCTCTAGACCAATCCCCAAGCAGAATAACTGCCATTGATCTTGATGGTGCGGGCTATGAGGGGTTCATAACATCAGAAATTGGGAACCTAACCGAGCTCACCTACATCAATCTAAGTAAAAACAACTTTCGAGGACCACTACCCGAATCCATTTCCAATCTAAAGAAGCTCACTAGACTTTCACTGTCCAACAATTTCTTCACGGGCGGCATTCCCTTGGGATTGAACACACTCAAAGAGCTAGAATCTCTGGACATTTCGTTCAATAAGCTTTCTGGTACAATACGCTCCAGCATCGGTGGATTAAGAGGCTTGACATTCTTAAGCCTGTCCAACAATAGATTCACTGGCAGAATCCCAGACCTCTCGGGGTTATGGCAGTTGAAGACATTAGATCTCAGCAGAAACCAGCTCTTCGGGAGTCTGCCAAATTTTCCAATAAGCTTGAGAACCTTAATTCTGAGCCATAACATACTTTCTGGGTCCCTTACACCTCTAATCATACTCAAAAACCTCAGAGTGCTAGATGTGAGCAACAACAGACTTTCAGGTGCTATAACTAGAGAGTTATTTACCATACCCAAGATGACTCGACTCAACGTTTCAAATAACCACTTCAAATTGTTAGATATCATTAGATCTGTTGGACACGGAGCACAGCTTGAGGTGCTTGATGCTCAACGAAATCAGCTGCGAGGACATTTGCCTGTAAATTTGGTAACCCTGCAGAATCTAACAACAATCGACCTTTCCTACAACCAGTTCTTTGGTCATATACCCGAGGAATATGGAGCAAGAGTCGGAGCCTGGAGAACGCTCTACCTAGATCACAACGATTTGCTAGGAAGGCTACCAGCGGAATTCATTAAAAGACCAGATAGAATCAAGGGTAGTCTTTCAAACAACTGCCTACAGTGTCCGGACAACATTCCATTGTGCACCGGTCGCCAGAGGAAAATATCAGAATGTCACGAACACACAAACACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8

Protein Analysis

406

Amino Acids

45.31

Weight (kDa)

8.83

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 100 - 181 2.8e-06 Leucine-rich repeat region
LRR_4 PF12799 131 - 167 1e-05 Leucine Rich repeats (2 copies)
LRR_8 PF13855 156 - 214 2.7e-07 Leucine rich repeat
LRR_4 PF12799 181 - 221 1.2e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59510
fragaria_vesca FvH4_4g10920
prunus_persica Prupe.1G197000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0406451
rosa_laevigata RLG00000008770
rosa_multiflora Rmu_ssc0000186.1_g000018
rosa_roxburghii Rroxscaffold_5G00351330
rosa_rugosa Rorug04G0071400
rosa_samantha Rh4BG135600 Rh4CG146000 Rh4DG133100
rosa_wichuraiana Rw4G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 4 cut(s) 198, 278, 435, 1085
AclI AACGTT 1 cut(s) 828
AclWI GGATC 2 cut(s) 132, 173
AcsI RAATTY 4 cut(s) 145, 658, 940, 1088
AfaI GTAC 1 cut(s) 508
AflII CTTAAG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 1169
AgsI TTSAA 7 cut(s) 95, 454, 493, 613, 834, 847, 1128
AjnI CCWGG 1 cut(s) 1034
AjuI GAANNNNNNNTTGG 4 cut(s) 410, 442, 554, 586
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
Alw21I GWGCWC 3 cut(s) 327, 886, 1169
Alw44I GTGCAC 1 cut(s) 1165
AlwI GGATC 2 cut(s) 132, 173
Ama87I CYCGRG 2 cut(s) 595, 1007
Aor13HI TCCGGA 1 cut(s) 1147
ApaLI GTGCAC 1 cut(s) 1165
ApeKI GCWGC 1 cut(s) 919
ApoI RAATTY 4 cut(s) 145, 658, 940, 1088
AsiGI ACCGGT 1 cut(s) 1169
AspS9I GGNCC 2 cut(s) 362, 711
AsuHPI GGTGA 1 cut(s) 319
AvaI CYCGRG 2 cut(s) 595, 1007
AvaII GGWCC 2 cut(s) 362, 711
BaeGI GKGCMC 1 cut(s) 1169
BanII GRGCYC 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 3 cut(s) 327, 886, 1169
BbvI GCAGC 1 cut(s) 906
BccI CCATC 2 cut(s) 143, 266
BciT130I CCWGG 1 cut(s) 1036
BfaI CTAG 9 cut(s) 29, 66, 233, 402, 470, 752, 789, 1052, 1070
BfmI CTRYAG 3 cut(s) 192, 953, 1139
BfrI CTTAAG 1 cut(s) 550
BglII AGATCT 2 cut(s) 622, 866
BisI GCNGC 2 cut(s) 436, 920
BlsI GCNGC 2 cut(s) 437, 921
Bme1390I CCNGG 1 cut(s) 1036
Bme18I GGWCC 2 cut(s) 362, 711
BmeT110I CYCGRG 2 cut(s) 595, 1007
BmgT120I GGNCC 2 cut(s) 362, 711
BmiI GGNNCC 4 cut(s) 314, 712, 713, 1033
BmrFI CCNGG 1 cut(s) 1036
BmsI GCATC 3 cut(s) 3, 531, 892
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 2 cut(s) 502, 1057
BpuEI CTTGAG 2 cut(s) 694, 911
BsaJI CCNNGG 2 cut(s) 444, 1008
BsaWI WCCGGW 2 cut(s) 1147, 1169
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1011, 1041
Bse118I RCCGGY 1 cut(s) 1169
Bse1I ACTGG 2 cut(s) 580, 988
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 1 cut(s) 1036
BseDI CCNNGG 2 cut(s) 444, 1008
BseGI GGATG 1 cut(s) 135
BseMII CTCAG 4 cut(s) 146, 640, 680, 757
BseNI ACTGG 2 cut(s) 580, 988
BseSI GKGCMC 1 cut(s) 1169
BseXI GCAGC 1 cut(s) 906
BseYI CCCAGC 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 1174
BshTI ACCGGT 1 cut(s) 1169
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 3 cut(s) 327, 886, 1169
BsiHKCI CYCGRG 2 cut(s) 595, 1007
BsiSI CCGG 2 cut(s) 1148, 1170
BslFI GGGAC 2 cut(s) 179, 697
BsmFI GGGAC 2 cut(s) 179, 697
BsmI GAATGC 1 cut(s) 438
BsoBI CYCGRG 2 cut(s) 595, 1007
Bsp1286I GDGCHC 3 cut(s) 327, 886, 1169
Bsp13I TCCGGA 1 cut(s) 1147
Bsp143I GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
BspACI CCGC 4 cut(s) 198, 278, 435, 1085
BspCNI CTCAG 4 cut(s) 147, 639, 681, 756
BspEI TCCGGA 1 cut(s) 1147
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 4 cut(s) 314, 712, 713, 1033
BspMAI CTGCAG 1 cut(s) 957
BspPI GGATC 2 cut(s) 132, 173
BspQI GCTCTTC 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 1169
BsrI ACTGG 2 cut(s) 580, 988
BssAI RCCGGY 1 cut(s) 1169
BssECI CCNNGG 2 cut(s) 444, 1008
BssMI GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 1036
Bst4CI ACNGT 3 cut(s) 43, 414, 1143
Bst6I CTCTTC 1 cut(s) 647
BstAFI CTTAAG 1 cut(s) 550
BstC8I GCNNGC 3 cut(s) 8, 200, 280
BstDEI CTNAG 5 cut(s) 155, 341, 626, 689, 743
BstEII GGTNACC 1 cut(s) 946
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 6 cut(s) 127, 181, 268, 625, 869, 1057
BstMBI GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
BstMCI CGRYCG 1 cut(s) 1174
BstMWI GCNNNNNNNGC 3 cut(s) 895, 1075, 1173
BstNI CCWGG 1 cut(s) 1036
BstPI GGTNACC 1 cut(s) 946
BstSCI CCNGG 1 cut(s) 1034
BstSFI CTRYAG 3 cut(s) 192, 953, 1139
BstSLI GKGCMC 1 cut(s) 1169
BstV1I GCAGC 1 cut(s) 906
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 3 cut(s) 124, 622, 866
BstXI CCANNNNNNTGG 1 cut(s) 995
BstYI RGATCY 3 cut(s) 124, 622, 866
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 410, 573, 1148
Cac8I GCNNGC 3 cut(s) 8, 200, 280
CciI TCATGA 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 1169
Cfr13I GGNCC 2 cut(s) 362, 711
Csp6I GTAC 1 cut(s) 507
CspAI ACCGGT 1 cut(s) 1169
CviAII CATG 4 cut(s) 104, 109, 121, 183
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 5 cut(s) 155, 341, 626, 689, 743
DpnI GATC 6 cut(s) 126, 180, 267, 624, 868, 1056
DpnII GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 2 cut(s) 362, 711
Eco53kI GAGCTC 1 cut(s) 325
Eco88I CYCGRG 2 cut(s) 595, 1007
Eco91I GGTNACC 1 cut(s) 946
EcoICRI GAGCTC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 711
EcoO65I GGTNACC 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 1034
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 4 cut(s) 107, 112, 124, 186
FaqI GGGAC 2 cut(s) 179, 697
FatI CATG 4 cut(s) 103, 108, 120, 182
FauI CCCGC 2 cut(s) 191, 271
Fnu4HI GCNGC 2 cut(s) 436, 920
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 2 cut(s) 436, 920
FspBI CTAG 9 cut(s) 29, 66, 233, 402, 470, 752, 789, 1052, 1070
GluI GCNGC 2 cut(s) 436, 920
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 2 cut(s) 502, 1057
HapII CCGG 2 cut(s) 1148, 1170
Hin1II CATG 4 cut(s) 107, 112, 124, 186
HindIII AAGCTT 2 cut(s) 497, 670
HpaII CCGG 2 cut(s) 1148, 1170
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 63, 1167
Hpy188I TCNGA 8 cut(s) 88, 156, 212, 304, 690, 746, 1031, 1192
Hpy188III TCNNGA 7 cut(s) 109, 233, 269, 479, 646, 1148, 1199
Hpy8I GTNNAC 2 cut(s) 63, 1167
HpyAV CCTTC 1 cut(s) 1068
HpyCH4III ACNGT 3 cut(s) 43, 414, 1143
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 4 cut(s) 120, 222, 955, 1167
HpyF10VI GCNNNNNNNGC 3 cut(s) 895, 1075, 1173
HpyF3I CTNAG 5 cut(s) 155, 341, 626, 689, 743
HpySE526I ACGT 1 cut(s) 828
Hsp92II CATG 4 cut(s) 107, 112, 124, 186
KflI GGGWCCC 1 cut(s) 711
Kpn2I TCCGGA 1 cut(s) 1147
Kzo9I GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
LguI GCTCTTC 1 cut(s) 647
LmnI GCTCC 4 cut(s) 521, 881, 1019, 1031
Lsp1109I GCAGC 1 cut(s) 906
LweI GCATC 3 cut(s) 3, 531, 892
MaeI CTAG 9 cut(s) 29, 66, 233, 402, 470, 752, 789, 1052, 1070
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 2 cut(s) 946, 1196
MalI GATC 6 cut(s) 126, 180, 267, 624, 868, 1056
MboI GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
MboII GAAGA 4 cut(s) 92, 418, 625, 634
MfeI CAATTG 1 cut(s) 1216
MflI RGATCY 3 cut(s) 124, 622, 866
MhlI GDGCHC 3 cut(s) 327, 886, 1169
MluCI AATT 9 cut(s) 145, 306, 421, 658, 684, 848, 940, 1088, 1216
MlyI GAGTC 5 cut(s) 222, 658, 811, 816, 1035
MmeI TCCRAC 4 cut(s) 441, 585, 853, 1009
MroI TCCGGA 1 cut(s) 1147
MseI TTAA 4 cut(s) 533, 551, 683, 1095
MspA1I CMGCKG 2 cut(s) 919, 1085
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 2 cut(s) 1148, 1170
MspR9I CCNGG 1 cut(s) 1036
MunI CAATTG 1 cut(s) 1216
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 1 cut(s) 1036
MwoI GCNNNNNNNGC 3 cut(s) 895, 1075, 1173
NdeII GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
NlaIII CATG 4 cut(s) 107, 112, 124, 186
NlaIV GGNNCC 4 cut(s) 314, 712, 713, 1033
NmuCI GTSAC 1 cut(s) 1196
PagI TCATGA 1 cut(s) 108
PciSI GCTCTTC 1 cut(s) 647
PctI GAATGC 1 cut(s) 438
PfeI GAWTC 6 cut(s) 374, 473, 570, 582, 958, 1110
PinAI ACCGGT 1 cut(s) 1169
PkrI GCNGC 2 cut(s) 437, 921
PleI GAGTC 5 cut(s) 221, 657, 811, 816, 1034
PpsI GAGTC 5 cut(s) 221, 657, 811, 816, 1034
PpuMI RGGWCCY 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 1034
PspEI GGTNACC 1 cut(s) 946
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 1 cut(s) 1034
PspN4I GGNNCC 4 cut(s) 314, 712, 713, 1033
PspPI GGNCC 2 cut(s) 362, 711
PspPPI RGGWCCY 1 cut(s) 711
PstI CTGCAG 1 cut(s) 957
PsuI RGATCY 3 cut(s) 124, 622, 866
PvuII CAGCTG 1 cut(s) 919
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 647
SaqAI TTAA 4 cut(s) 533, 551, 683, 1095
SatI GCNGC 2 cut(s) 436, 920
Sau3AI GATC 6 cut(s) 124, 178, 265, 622, 866, 1054
Sau96I GGNCC 2 cut(s) 362, 711
SchI GAGTC 5 cut(s) 222, 658, 811, 816, 1035
ScrFI CCNGG 1 cut(s) 1036
SduI GDGCHC 3 cut(s) 327, 886, 1169
SfaNI GCATC 3 cut(s) 3, 531, 892
SfcI CTRYAG 3 cut(s) 192, 953, 1139
SinI GGWCC 2 cut(s) 362, 711
SmlI CTYRAG 3 cut(s) 550, 673, 890
SmoI CTYRAG 3 cut(s) 550, 673, 890
Sse9I AATT 9 cut(s) 145, 306, 421, 658, 684, 848, 940, 1088, 1216
SsiI CCGC 4 cut(s) 198, 278, 435, 1085
SspMI CTAG 9 cut(s) 29, 66, 233, 402, 470, 752, 789, 1052, 1070
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 1034
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 3 cut(s) 43, 414, 1143
TaiI ACGT 1 cut(s) 831
TaqI TCGA 3 cut(s) 358, 820, 972
TasI AATT 9 cut(s) 145, 306, 421, 658, 684, 848, 940, 1088, 1216
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 6 cut(s) 374, 473, 570, 582, 958, 1110
Tru1I TTAA 4 cut(s) 533, 551, 683, 1095
Tru9I TTAA 4 cut(s) 533, 551, 683, 1095
TscAI CASTG 3 cut(s) 417, 580, 1148
TseFI GTSAC 1 cut(s) 1196
TseI GCWGC 1 cut(s) 919
Tsp45I GTSAC 1 cut(s) 1196
TspDTI ATGAA 5 cut(s) 92, 125, 160, 283, 1081
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 3 cut(s) 417, 580, 1148
Vha464I CTTAAG 1 cut(s) 550
VneI GTGCAC 1 cut(s) 1165
VpaK11BI GGWCC 2 cut(s) 362, 711
XapI RAATTY 4 cut(s) 145, 658, 940, 1088
XbaI TCTAGA 1 cut(s) 232
XspI CTAG 9 cut(s) 29, 66, 233, 402, 470, 752, 789, 1052, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.