Rw5G016860

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0025544
Physical Location & Seq
Forward (+)
21501019 .. 21509748
8730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016860.1

Sequence Viewer

Length: 783 bp
ATGAGGTGGCCCTCCAAAGATCCATCATCCATGGATTCCATGCATCGTGTTGAAGGAAACAATACCGTTACGACTTATTCAAGACAACTGGCATATCCTATTATCGTTGTACATTTCAAGCTTAATCCTAACATCAATGTACATTTTGCAACTTTCCAATCTCCTGAAAACCTTCCAACACAACTTCCTGGCCGTAGCTATGCTTCCCCCAATCACCCTCCTACCAATTTAAGCCACATTCTGATCGGAATGGTTGGTTCGGTCAACACCTTGAAGCACAGAACATCATACATTGAAGCATGGTGGCGGCCCAATGTCACTCGAGGATGCCTCTTTTTGGATCCTCCTACTGCAGCGTTTTTGTCTTGGCCTTCATCCTATCCTCCTTTTCGTGTCAATGAGGACATCACTAGATTAAAAGTGTACCCGAAAATTTTGAATACGGTCCAAGCACGCATAGTGCGTTCAATTTTGGAGATATTTACAGAAGGTGACAAAGATATGAATCGCGATGACGACAACGACGACTTAAGTCAACTTGCTGCTATGGTGGTGGTTCCTGCTGCCGCCCAGCCCAAGCGTGATATGTCTGCCAAGTTCCCCTCCAAGCCTCTCCCTCCTGCTCAAGCTGGTTGTTTACCAACTTTGGCCACAATACCAGTCTGGATAGGTCTACTCCAAGCTCTTTCGAATGTGATGAAGACTCATGCAAGAGCGAACATACGTTCCATGACACTATCAAGCTTTACTCGTGTCGCCTTTGGCATTGAGTCGGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

260

Amino Acids

29.02

Weight (kDa)

9.57

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025544)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002839.1_g000002
rosa_samantha Rh5AG185800
rosa_wichuraiana Rw5G016860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 673
AccII CGCG 1 cut(s) 510
AciI CCGC 2 cut(s) 307, 567
AclWI GGATC 3 cut(s) 14, 335, 348
AcoI YGGCCR 2 cut(s) 190, 648
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 3 cut(s) 111, 141, 425
AfiI CCNNNNNNNGG 1 cut(s) 337
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 7 cut(s) 53, 81, 118, 274, 296, 439, 468
AjnI CCWGG 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
AluBI AGCT 5 cut(s) 121, 198, 629, 683, 744
AluI AGCT 5 cut(s) 121, 198, 629, 683, 744
AlwI GGATC 3 cut(s) 14, 335, 348
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 5 cut(s) 8, 190, 308, 368, 648
ApeKI GCWGC 3 cut(s) 353, 542, 563
ApoI RAATTY 1 cut(s) 432
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 3 cut(s) 9, 309, 445
AsuHPI GGTGA 2 cut(s) 206, 503
AsuII TTCGAA 1 cut(s) 689
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 1 cut(s) 445
BalI TGGCCA 1 cut(s) 650
BamHI GGATCC 1 cut(s) 340
BarI GAAGNNNNNNTAC 2 cut(s) 187, 219
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 707
BbvI GCAGC 3 cut(s) 365, 529, 550
BccI CCATC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 189
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 529
BisI GCNGC 5 cut(s) 308, 354, 543, 564, 567
BlsI GCNGC 5 cut(s) 309, 355, 544, 565, 568
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 9, 309, 445
BmiI GGNNCC 2 cut(s) 342, 558
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 52, 317
BoxI GACNNNNGTC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 707
BplI GAGNNNNNCTC 2 cut(s) 315, 347
Bpu14I TTCGAA 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 93, 659
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 3 cut(s) 26, 332, 374
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 337
BseNI ACTGG 2 cut(s) 93, 659
BseXI GCAGC 3 cut(s) 365, 529, 550
BseYI CCCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 5 cut(s) 10, 192, 310, 370, 650
BsiHKCI CYCGRG 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 337
BsnI GGCC 5 cut(s) 10, 192, 310, 370, 650
BsoBI CYCGRG 1 cut(s) 321
Bsp119I TTCGAA 1 cut(s) 689
Bsp1407I TGTACA 2 cut(s) 109, 139
Bsp143I GATC 3 cut(s) 19, 243, 340
Bsp19I CCATGG 1 cut(s) 30
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 2 cut(s) 307, 567
BspANI GGCC 5 cut(s) 10, 192, 310, 370, 650
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 342, 558
BspMAI CTGCAG 1 cut(s) 355
BspPI GGATC 3 cut(s) 14, 335, 348
BspT104I TTCGAA 1 cut(s) 689
BspTI CTTAAG 1 cut(s) 529
BsrGI TGTACA 2 cut(s) 109, 139
BsrI ACTGG 2 cut(s) 93, 659
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 19, 243, 340
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 1 cut(s) 30
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 67, 445
BstAFI CTTAAG 1 cut(s) 529
BstAUI TGTACA 2 cut(s) 109, 139
BstBI TTCGAA 1 cut(s) 689
BstC8I GCNNGC 1 cut(s) 454
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 26, 332, 374
BstFNI CGCG 1 cut(s) 510
BstKTI GATC 3 cut(s) 22, 246, 343
BstMBI GATC 3 cut(s) 19, 243, 340
BstNI CCWGG 1 cut(s) 189
BstPAI GACNNNNGTC 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 351
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 3 cut(s) 365, 529, 550
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 2 cut(s) 19, 340
BstYI RGATCY 2 cut(s) 19, 340
BsuRI GGCC 5 cut(s) 10, 192, 310, 370, 650
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 3 cut(s) 26, 332, 374
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 1 cut(s) 454
Cfr13I GGNCC 3 cut(s) 9, 309, 445
Csp6I GTAC 3 cut(s) 110, 140, 424
CviAII CATG 5 cut(s) 31, 40, 300, 707, 730
CviQI GTAC 3 cut(s) 110, 140, 424
DpnI GATC 3 cut(s) 21, 245, 342
DpnII GATC 3 cut(s) 19, 243, 340
EaeI YGGCCR 2 cut(s) 190, 648
Eco130I CCWWGG 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 445
Eco88I CYCGRG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 5 cut(s) 34, 43, 303, 710, 733
FatI CATG 5 cut(s) 30, 39, 299, 706, 729
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 5 cut(s) 308, 354, 543, 564, 567
FokI GGATG 3 cut(s) 13, 339, 361
Fsp4HI GCNGC 5 cut(s) 308, 354, 543, 564, 567
FspBI CTAG 1 cut(s) 411
GluI GCNGC 5 cut(s) 308, 354, 543, 564, 567
GsaI CCCAGC 1 cut(s) 574
HaeIII GGCC 5 cut(s) 10, 192, 310, 370, 650
Hin1II CATG 5 cut(s) 34, 43, 303, 710, 733
HincII GTYRAC 2 cut(s) 265, 536
HindII GTYRAC 2 cut(s) 265, 536
HindIII AAGCTT 2 cut(s) 119, 742
HinfI GANTC 4 cut(s) 35, 505, 703, 770
HphI GGTGA 2 cut(s) 206, 503
Hpy166II GTNNAC 5 cut(s) 265, 424, 536, 638, 674
Hpy188I TCNGA 2 cut(s) 243, 248
Hpy188III TCNNGA 4 cut(s) 81, 164, 509, 664
Hpy8I GTNNAC 5 cut(s) 265, 424, 536, 638, 674
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 4 cut(s) 47, 182, 381, 482
HpyCH4III ACNGT 2 cut(s) 67, 445
HpyCH4IV ACGT 1 cut(s) 724
HpyCH4V TGCA 4 cut(s) 43, 149, 353, 710
HpySE526I ACGT 1 cut(s) 724
Hsp92II CATG 5 cut(s) 34, 43, 303, 710, 733
Kzo9I GATC 3 cut(s) 19, 243, 340
Lsp1109I GCAGC 3 cut(s) 365, 529, 550
LweI GCATC 2 cut(s) 52, 317
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 724
MaeIII GTNAC 3 cut(s) 67, 316, 491
MalI GATC 3 cut(s) 21, 245, 342
MboI GATC 3 cut(s) 19, 243, 340
MboII GAAGA 1 cut(s) 712
MflI RGATCY 2 cut(s) 19, 340
MlsI TGGCCA 1 cut(s) 650
MluCI AATT 3 cut(s) 226, 432, 468
MluNI TGGCCA 1 cut(s) 650
MlyI GAGTC 2 cut(s) 697, 779
MmeI TCCRAC 1 cut(s) 200
Mox20I TGGCCA 1 cut(s) 650
Mph1103I ATGCAT 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 1 cut(s) 650
MseI TTAA 5 cut(s) 123, 230, 416, 530, 781
Msp20I TGGCCA 1 cut(s) 650
MspCI CTTAAG 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 510
NcoI CCATGG 1 cut(s) 30
NdeII GATC 3 cut(s) 19, 243, 340
NlaIII CATG 5 cut(s) 34, 43, 303, 710, 733
NlaIV GGNNCC 2 cut(s) 342, 558
NmuCI GTSAC 2 cut(s) 316, 491
NruI TCGCGA 1 cut(s) 510
NsiI ATGCAT 1 cut(s) 45
NspV TTCGAA 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 460, 522
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 35, 505
PkrI GCNGC 5 cut(s) 309, 355, 544, 565, 568
PleI GAGTC 2 cut(s) 697, 778
PpsI GAGTC 2 cut(s) 697, 778
PshAI GACNNNNGTC 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 342, 558
PspPI GGNCC 3 cut(s) 9, 309, 445
PspXI VCTCGAGB 1 cut(s) 321
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 2 cut(s) 19, 340
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 3 cut(s) 111, 141, 425
RsaNI GTAC 3 cut(s) 110, 140, 424
SaqAI TTAA 5 cut(s) 123, 230, 416, 530, 781
SatI GCNGC 5 cut(s) 308, 354, 543, 564, 567
Sau3AI GATC 3 cut(s) 19, 243, 340
Sau96I GGNCC 3 cut(s) 9, 309, 445
SchI GAGTC 2 cut(s) 697, 779
ScrFI CCNGG 1 cut(s) 189
SfaNI GCATC 2 cut(s) 52, 317
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 689
SinI GGWCC 1 cut(s) 445
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 3 cut(s) 321, 529, 624
SmoI CTYRAG 3 cut(s) 321, 529, 624
Sse9I AATT 3 cut(s) 226, 432, 468
SsiI CCGC 2 cut(s) 307, 567
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 67, 445
TaiI ACGT 1 cut(s) 727
TaqI TCGA 2 cut(s) 322, 689
TaqII GACCGA 1 cut(s) 250
TasI AATT 3 cut(s) 226, 432, 468
TatI WGTACW 2 cut(s) 109, 139
TauI GCSGC 2 cut(s) 310, 569
TfiI GAWTC 2 cut(s) 35, 505
Tru1I TTAA 5 cut(s) 123, 230, 416, 530, 781
Tru9I TTAA 5 cut(s) 123, 230, 416, 530, 781
TseFI GTSAC 2 cut(s) 316, 491
TseI GCWGC 3 cut(s) 353, 542, 563
Tsp45I GTSAC 2 cut(s) 316, 491
TspDTI ATGAA 3 cut(s) 363, 518, 713
Vha464I CTTAAG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 1 cut(s) 432
XhoI CTCGAG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.