Rw6G003120

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0031064
Physical Location & Seq
Forward (+)
5014754 .. 5024396
9643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003120.1

Sequence Viewer

Length: 576 bp
ATGGAGGAGGAGAAAAGTACCGATGTCGTCATTGTCGGCGCCGGAGTTGCTAGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTACATGTAATTGAAAGAGACTTGAGTGAGCCAGACAGAATCGTTGGGGAGCTGTTGCAGCCAGGGGGATATCTCAAATTGATTGAGTTGGGTCTTGAAGAATTCCATTATAGTGTGAAAATGGAACAAGGATCAGTGACAACACTAATAGAGGAAAAGGGCATTATTAAAGGGGTGATTTACAAGAACAAGACTGGAGAGGAGATGAGAACATATGCTCCTCTAACAATCGTGTGCGATGGCTATTTTTCAAATTTGTGCAAATCTCTTAGTGCTCCAAAGGTTGAAAGTCCATCTTGTTTTGTTGGTTTGATCTTGGAGGACTGTGAGCTTCATGGGCATGTCATTCTGGGAGACCCTTCACCAATCCTGTTTTATCCCATCAGTTCCACTGAGGTTCGCTGTTTGGTAGATGTGCCTGGAACAAAAGTACCTTCACTAGCTAGTGGTGAAATGGCCAACTATTTGAAGCCCCTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

191

Amino Acids

20.83

Weight (kDa)

5.08

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 8 - 38 6.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 11 - 37 5.5e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 104 - 188 1.7e-29 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031064)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000345.1_g000002
rosa_wichuraiana Rw6G003120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 2 cut(s) 194, 346
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 19, 96, 525
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 5 cut(s) 107, 191, 345, 380, 562
AjnI CCWGG 2 cut(s) 154, 511
AloI GAACNNNNNNTCC 2 cut(s) 295, 327
AluBI AGCT 4 cut(s) 59, 145, 424, 536
AluI AGCT 4 cut(s) 59, 145, 424, 536
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 2 cut(s) 105, 441
AlwI GGATC 1 cut(s) 232
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 2 cut(s) 56, 151
ApoI RAATTY 2 cut(s) 194, 346
AspLEI GCGC 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 280, 447, 554
AxyI CCTNAGG 1 cut(s) 569
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BalI TGGCCA 1 cut(s) 551
BanI GGYRCC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 68, 163
BccI CCATC 3 cut(s) 326, 394, 482
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 2 cut(s) 156, 513
BcoDI GTCTC 2 cut(s) 105, 441
BfaI CTAG 3 cut(s) 51, 533, 537
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 2 cut(s) 57, 152
BlsI GCNGC 2 cut(s) 58, 153
Bme1390I CCNGG 2 cut(s) 156, 513
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 2 cut(s) 156, 513
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 136
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 78, 155
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bse1I ACTGG 1 cut(s) 292
Bse21I CCTNAGG 1 cut(s) 569
BseBI CCWGG 2 cut(s) 156, 513
BseDI CCNNGG 2 cut(s) 78, 155
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 4 cut(s) 20, 23, 303, 308
BseXI GCAGC 2 cut(s) 68, 163
BsgI GTGCAG 1 cut(s) 75
BshFI GGCC 1 cut(s) 551
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 42
BsmAI GTCTC 2 cut(s) 105, 441
BsnI GGCC 1 cut(s) 551
Bso31I GGTCTC 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 94
Bsp143I GATC 2 cut(s) 224, 405
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 441
BsrGI TGTACA 1 cut(s) 94
BsrI ACTGG 1 cut(s) 292
BssECI CCNNGG 2 cut(s) 78, 155
BssMI GATC 2 cut(s) 224, 405
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 2 cut(s) 156, 513
Bst4CI ACNGT 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 94
BstDEI CTNAG 3 cut(s) 362, 486, 569
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 2 cut(s) 227, 408
BstMAI GTCTC 2 cut(s) 105, 441
BstMBI GATC 2 cut(s) 224, 405
BstMWI GCNNNNNNNGC 5 cut(s) 47, 56, 62, 151, 430
BstNI CCWGG 2 cut(s) 156, 513
BstNSI RCATGY 2 cut(s) 101, 437
BstSCI CCNGG 2 cut(s) 154, 511
BstV1I GCAGC 2 cut(s) 68, 163
Bsu36I CCTNAGG 1 cut(s) 569
BsuRI GGCC 1 cut(s) 551
BtgZI GCGATG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 234, 483
CfoI GCGC 1 cut(s) 41
Csp6I GTAC 3 cut(s) 18, 95, 524
CviAII CATG 3 cut(s) 98, 428, 434
CviJI RGCY 9 cut(s) 59, 124, 145, 154, 336, 424, 536, 551, 565
CviKI_1 RGCY 9 cut(s) 59, 124, 145, 154, 336, 424, 536, 551, 565
CviQI GTAC 3 cut(s) 18, 95, 524
DdeI CTNAG 3 cut(s) 362, 486, 569
DinI GGCGCC 1 cut(s) 40
DpnI GATC 2 cut(s) 226, 407
DpnII GATC 2 cut(s) 224, 405
EaeI YGGCCR 1 cut(s) 549
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 441
Eco32I GATATC 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 569
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 154, 511
EcoRV GATATC 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 78
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 101, 431, 437
FaiI YATR 6 cut(s) 99, 204, 307, 309, 429, 435
FalI AAGNNNNNCTT 2 cut(s) 373, 405
FatI CATG 3 cut(s) 97, 427, 433
FauNDI CATATG 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 57, 152
Fsp4HI GCNGC 2 cut(s) 57, 152
FspBI CTAG 3 cut(s) 51, 533, 537
GlaI GCGC 1 cut(s) 40
GluI GCNGC 2 cut(s) 57, 152
GsuI CTGGAG 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 1 cut(s) 551
HapII CCGG 1 cut(s) 42
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 3 cut(s) 101, 431, 437
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HinfI GANTC 1 cut(s) 132
HpaII CCGG 1 cut(s) 42
HphI GGTGA 3 cut(s) 280, 447, 554
Hpy166II GTNNAC 1 cut(s) 95
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 77, 462, 537
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 3 cut(s) 56, 151, 354
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 56, 62, 151, 430
HpyF3I CTNAG 3 cut(s) 362, 486, 569
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 3 cut(s) 101, 431, 437
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 224, 405
LmnI GCTCC 3 cut(s) 142, 316, 373
Lsp1109I GCAGC 2 cut(s) 68, 163
MaeI CTAG 3 cut(s) 51, 533, 537
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 226, 407
MboI GATC 2 cut(s) 224, 405
MboII GAAGA 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 370
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 4 cut(s) 102, 170, 194, 346
MluNI TGGCCA 1 cut(s) 551
Mly113I GGCGCC 1 cut(s) 39
MnlI CCTC 5 cut(s) 238, 286, 324, 406, 481
Mox20I TGGCCA 1 cut(s) 551
MscI TGGCCA 1 cut(s) 551
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 204, 432
Msp20I TGGCCA 1 cut(s) 551
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 156, 513
MvaI CCWGG 2 cut(s) 156, 513
MwoI GCNNNNNNNGC 5 cut(s) 47, 56, 62, 151, 430
NarI GGCGCC 1 cut(s) 39
NdeI CATATG 1 cut(s) 307
NdeII GATC 2 cut(s) 224, 405
NlaIII CATG 3 cut(s) 101, 431, 437
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 2 cut(s) 101, 437
PciI ACATGT 1 cut(s) 97
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 2 cut(s) 58, 153
PluTI GGCGCC 1 cut(s) 42
PscI ACATGT 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 154, 511
PspGI CCWGG 2 cut(s) 154, 511
PspN4I GGNNCC 1 cut(s) 40
RsaI GTAC 3 cut(s) 19, 96, 525
RsaNI GTAC 3 cut(s) 18, 95, 524
RseI CAYNNNNRTG 2 cut(s) 204, 432
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 2 cut(s) 57, 152
Sau3AI GATC 2 cut(s) 224, 405
ScrFI CCNGG 2 cut(s) 156, 513
SduI GDGCHC 1 cut(s) 370
SetI ASST 8 cut(s) 61, 147, 378, 426, 492, 529, 538, 575
SfoI GGCGCC 1 cut(s) 40
SmiMI CAYNNNNRTG 2 cut(s) 204, 432
SmlI CTYRAG 1 cut(s) 115
SmoI CTYRAG 1 cut(s) 115
Sse9I AATT 4 cut(s) 102, 170, 194, 346
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 3 cut(s) 51, 533, 537
StyD4I CCNGG 2 cut(s) 154, 511
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 419
TasI AATT 4 cut(s) 102, 170, 194, 346
TatI WGTACW 1 cut(s) 94
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 234, 490
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 2 cut(s) 56, 151
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 416
TspRI CASTG 2 cut(s) 234, 490
XapI RAATTY 2 cut(s) 194, 346
XceI RCATGY 2 cut(s) 101, 437
XspI CTAG 3 cut(s) 51, 533, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.