Rw7G023310

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0031069
Physical Location & Seq
Forward (+)
27957188 .. 27960878
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023310.1

Sequence Viewer

Length: 945 bp
ATGGAAAGGAAGCTAAAAATTAATCATATGGATCGGGGAGCTGTGCAGGCTACTTTTGGACCATTCAGGGCTTATGGAGCCTCGAATACCATGTTCACAAGTAATGATAGCTCCCTACATGCAACACAGAATATACTAAAAGAGAAGACTAGGAAGAAAATGAGTGAACGGGGATCATCTAGTGTAGCCAAAACTGCGGTAAACAAGGCAGAGAATGGAAATGAGAGAGGAAATTCTTCAAGAAACTCTGATATGCAGTGTGATCGCAACATGTCCGGCATTGGCAAGTTGGTGGATACAAAGAATACCATTCTCACAAGTAATGATATCTCTCTACATGCAACACAGAATATACTAAAAGAGAAGACTAGGAAGAAAATGAGTGAACGGGGATCATCTAGTGTAGCCAAAACTGCAGTAAACAAGGCACATAATAGAAATGAGAGAGGAAAATCTTCAAGAAACTCTGATATGCAGTGTGATCGCAACATGTCCGGCATTGGCAAGCTGGTGGATACAAAGAACATTGGTACATGTGACTTGAAACAGTCTGGCACTAGTGGTGTCAGCTCAGATTCGGGTGCAGAAATCTTGGAAGCAATGACGTCAATTGATGTTCCTGATCCTGATTTTCACAATTTTGATGAGGATCGAACAGAAAAGTCTTTTTTTAAAAATCAGGTCTGGGCCGCCTATGATGAGGTTAATGGGATGCCTCGGTATTATGCCAAAATTCAGAGTACAGTGTTTCGGCTTCATTTGGATCCCACAACTGTCATGATGAGAATTCCCAGAGAAGAGATGTTTAGATTGTCTCATCAAGTCCCTGCACACTTGCTTACAGGTGAAGAAGCTCTAAATGCTCCAAAAGGTTGTTGGGATCTTGATCCAGCAGCAACTCCGGCTGATCTTCTTGAGGTAATGACTGATGTTCAAGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

314

Amino Acids

34.86

Weight (kDa)

8.25

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 202 - 251 3.7e-17 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031069)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000394.1_g000044
rosa_wichuraiana Rw7G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 608
AciI CCGC 2 cut(s) 197, 690
AclWI GGATC 9 cut(s) 39, 181, 400, 617, 657, 758, 771, 881, 888
AcsI RAATTY 3 cut(s) 232, 732, 786
AcyI GRCGYC 1 cut(s) 605
AfaI GTAC 2 cut(s) 532, 742
AflIII ACRYGT 3 cut(s) 270, 489, 533
AgsI TTSAA 4 cut(s) 240, 459, 544, 935
AhlI ACTAGT 1 cut(s) 557
AluBI AGCT 6 cut(s) 13, 41, 111, 508, 570, 854
AluI AGCT 6 cut(s) 13, 41, 111, 508, 570, 854
Alw26I GTCTC 1 cut(s) 819
AlwI GGATC 9 cut(s) 39, 181, 400, 617, 657, 758, 771, 881, 888
AoxI GGCC 1 cut(s) 687
ApeKI GCWGC 1 cut(s) 893
ApoI RAATTY 3 cut(s) 232, 732, 786
AseI ATTAAT 1 cut(s) 21
Asp700I GAANNNNTTC 2 cut(s) 235, 454
AspS9I GGNCC 2 cut(s) 59, 687
AsuHPI GGTGA 1 cut(s) 857
AvaII GGWCC 1 cut(s) 59
BamHI GGATCC 1 cut(s) 763
BbsI GAAGAC 2 cut(s) 152, 371
BbvI GCAGC 1 cut(s) 905
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 464, 498
BciVI GTATCC 2 cut(s) 289, 508
BcoDI GTCTC 1 cut(s) 819
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 5 cut(s) 150, 180, 369, 399, 558
BfmI CTRYAG 1 cut(s) 414
BfuI GTATCC 2 cut(s) 289, 508
BisI GCNGC 2 cut(s) 690, 894
BlsI GCNGC 2 cut(s) 691, 895
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 687
BmiI GGNNCC 2 cut(s) 79, 765
BmsI GCATC 1 cut(s) 702
BpiI GAAGAC 2 cut(s) 152, 371
BpuEI CTTGAG 1 cut(s) 935
BsaBI GATNNNNATC 2 cut(s) 648, 885
BsaHI GRCGYC 1 cut(s) 605
BsaJI CCNNGG 1 cut(s) 716
Bse3DI GCAATG 1 cut(s) 606
Bse8I GATNNNNATC 2 cut(s) 648, 885
BseDI CCNNGG 1 cut(s) 716
BseGI GGATG 1 cut(s) 717
BseJI GATNNNNATC 2 cut(s) 648, 885
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 1 cut(s) 585
BseXI GCAGC 1 cut(s) 905
BsgI GTGCAG 3 cut(s) 65, 603, 813
BshFI GGCC 1 cut(s) 689
BsiSI CCGG 3 cut(s) 276, 495, 902
BslFI GGGAC 1 cut(s) 809
BsmAI GTCTC 1 cut(s) 819
BsmFI GGGAC 1 cut(s) 809
BsnI GGCC 1 cut(s) 689
BspACI CCGC 2 cut(s) 197, 690
BspANI GGCC 1 cut(s) 689
BspCNI CTCAG 1 cut(s) 584
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 79, 765
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 9 cut(s) 39, 181, 400, 617, 657, 758, 771, 881, 888
BsrDI GCAATG 1 cut(s) 606
BssECI CCNNGG 1 cut(s) 716
BssNI GRCGYC 1 cut(s) 605
Bst4CI ACNGT 3 cut(s) 549, 745, 775
Bst6I CTCTTC 1 cut(s) 792
BstACI GRCGYC 1 cut(s) 605
BstC8I GCNNGC 2 cut(s) 48, 506
BstDEI CTNAG 1 cut(s) 571
BstF5I GGATG 1 cut(s) 717
BstMAI GTCTC 1 cut(s) 819
BstMWI GCNNNNNNNGC 6 cut(s) 47, 77, 194, 413, 860, 902
BstNSI RCATGY 5 cut(s) 122, 274, 341, 493, 537
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 905
BstV2I GAAGAC 2 cut(s) 152, 371
BstX2I RGATCY 2 cut(s) 763, 880
BstYI RGATCY 2 cut(s) 763, 880
BsuI GTATCC 2 cut(s) 289, 508
BsuRI GGCC 1 cut(s) 689
BtsCI GGATG 1 cut(s) 717
BtsI GCAGTG 2 cut(s) 263, 482
BtsIMutI CAGTG 3 cut(s) 263, 482, 750
Cac8I GCNNGC 2 cut(s) 48, 506
CciI TCATGA 1 cut(s) 777
Cfr13I GGNCC 2 cut(s) 59, 687
Csp6I GTAC 2 cut(s) 531, 741
CviAII CATG 7 cut(s) 91, 119, 271, 338, 490, 534, 778
CviQI GTAC 2 cut(s) 531, 741
DdeI CTNAG 1 cut(s) 571
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 786
EcoRV GATATC 1 cut(s) 328
FaeI CATG 7 cut(s) 94, 122, 274, 341, 493, 537, 781
FaqI GGGAC 1 cut(s) 809
FatI CATG 7 cut(s) 90, 118, 270, 337, 489, 533, 777
FauNDI CATATG 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 690, 894
FokI GGATG 1 cut(s) 724
Fsp4HI GCNGC 2 cut(s) 690, 894
FspBI CTAG 5 cut(s) 150, 180, 369, 399, 558
GluI GCNGC 2 cut(s) 690, 894
HaeIII GGCC 1 cut(s) 689
HapII CCGG 3 cut(s) 276, 495, 902
Hin1I GRCGYC 1 cut(s) 605
Hin1II CATG 7 cut(s) 94, 122, 274, 341, 493, 537, 781
HinfI GANTC 1 cut(s) 575
HpaII CCGG 3 cut(s) 276, 495, 902
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 5 cut(s) 96, 167, 202, 386, 421
Hpy188I TCNGA 4 cut(s) 250, 469, 574, 738
Hpy188III TCNNGA 8 cut(s) 240, 459, 620, 626, 778, 884, 914, 935
Hpy8I GTNNAC 5 cut(s) 96, 167, 202, 386, 421
HpyCH4III ACNGT 3 cut(s) 549, 745, 775
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 8 cut(s) 46, 122, 256, 341, 416, 475, 584, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 77, 194, 413, 860, 902
HpyF3I CTNAG 1 cut(s) 571
HpySE526I ACGT 1 cut(s) 605
Hsp92I GRCGYC 1 cut(s) 605
Hsp92II CATG 7 cut(s) 94, 122, 274, 341, 493, 537, 781
LmnI GCTCC 4 cut(s) 38, 77, 116, 868
Lsp1109I GCAGC 1 cut(s) 905
LweI GCATC 1 cut(s) 702
MaeI CTAG 5 cut(s) 150, 180, 369, 399, 558
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 536
MboII GAAGA 9 cut(s) 157, 166, 228, 376, 385, 447, 809, 860, 902
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 2 cut(s) 763, 880
MluCI AATT 6 cut(s) 18, 232, 609, 637, 732, 786
MnlI CCTC 7 cut(s) 91, 221, 440, 640, 694, 726, 910
MroXI GAANNNNTTC 2 cut(s) 235, 454
MseI TTAA 3 cut(s) 21, 672, 705
MspI CCGG 3 cut(s) 276, 495, 902
MunI CAATTG 1 cut(s) 609
MwoI GCNNNNNNNGC 6 cut(s) 47, 77, 194, 413, 860, 902
NdeI CATATG 1 cut(s) 27
NlaIII CATG 7 cut(s) 94, 122, 274, 341, 493, 537, 781
NlaIV GGNNCC 2 cut(s) 79, 765
NmuCI GTSAC 1 cut(s) 536
NspI RCATGY 5 cut(s) 122, 274, 341, 493, 537
PagI TCATGA 1 cut(s) 777
PciI ACATGT 3 cut(s) 270, 489, 533
PdmI GAANNNNTTC 2 cut(s) 235, 454
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 2 cut(s) 691, 895
PscI ACATGT 3 cut(s) 270, 489, 533
PshBI ATTAAT 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 79, 765
PspPI GGNCC 2 cut(s) 59, 687
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 2 cut(s) 763, 880
RsaI GTAC 2 cut(s) 532, 742
RsaNI GTAC 2 cut(s) 531, 741
SaqAI TTAA 3 cut(s) 21, 672, 705
SatI GCNGC 2 cut(s) 690, 894
Sau96I GGNCC 2 cut(s) 59, 687
SfaNI GCATC 1 cut(s) 702
SfcI CTRYAG 1 cut(s) 414
SinI GGWCC 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SpeI ACTAGT 1 cut(s) 557
Sse9I AATT 6 cut(s) 18, 232, 609, 637, 732, 786
SsiI CCGC 2 cut(s) 197, 690
SspMI CTAG 5 cut(s) 150, 180, 369, 399, 558
TaaI ACNGT 3 cut(s) 549, 745, 775
TaiI ACGT 1 cut(s) 608
TaqI TCGA 2 cut(s) 83, 652
TasI AATT 6 cut(s) 18, 232, 609, 637, 732, 786
TatI WGTACW 1 cut(s) 740
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 3 cut(s) 21, 672, 705
Tru9I TTAA 3 cut(s) 21, 672, 705
TscAI CASTG 3 cut(s) 263, 482, 750
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 1 cut(s) 893
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 1 cut(s) 746
TspRI CASTG 3 cut(s) 263, 482, 750
VpaK11BI GGWCC 1 cut(s) 59
VspI ATTAAT 1 cut(s) 21
XapI RAATTY 3 cut(s) 232, 732, 786
XceI RCATGY 5 cut(s) 122, 274, 341, 493, 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmnI GAANNNNTTC 2 cut(s) 235, 454
XspI CTAG 5 cut(s) 150, 180, 369, 399, 558
ZraI GACGTC 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.