Rw7G031770

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0025570
Physical Location & Seq
Forward (+)
45705827 .. 45706318
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031770.1

Sequence Viewer

Length: 492 bp
ATGGCTTCTCCTCAGCAAAAGGCACAAGCAAATTTCCAAGACTTGATGCCCACTATGGCTGACAAGGTCGGCGGGGATGGTTTGATCGGCAAGATCTGTAATGGGTTCAATCTTTTGACGGATCCCGTCAAGGGGGTCATCACGTTCGAGAACCTCAAGAGGAACTCGACTCTTCTAGGCTTGCAGAACTTAAGCGACAACGATCTACTGTCCAAGTTGCAGGAGGGCGATTTTGACAGCCACTGGGTTTTGGGCTTTGCGAGCTGGGATTGGTGCTCCGGCCACTGGATTCGGGGCTTTACGAGCTTTACGACGCCGATCTCGTCGTACTTCTTGGTTTTGTGCGTGATTCTGGTGCTTATGGGTCCAATGAAGAGTTTTGGATTTTTGTTGAAGAATCCAATTGTCAAGGATTTGTTATTGTTGTTGATTTATGGTGTTGGAAAGAACTGGTTTTGGATCGAAGAGTTGAAATGGATGTATATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03420 map03420
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

163

Amino Acids

18.42

Weight (kDa)

6.06

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025570)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003876.1_g000015
rosa_samantha Rh7DG376500
rosa_wichuraiana Rw7G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 72
AclWI GGATC 3 cut(s) 116, 129, 467
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 3 cut(s) 131, 132, 285
AflII CTTAAG 1 cut(s) 190
AgsI TTSAA 3 cut(s) 109, 394, 472
AluBI AGCT 2 cut(s) 264, 306
AluI AGCT 2 cut(s) 264, 306
Alw21I GWGCWC 1 cut(s) 278
AlwI GGATC 3 cut(s) 116, 129, 467
AlwNI CAGNNNCTG 1 cut(s) 243
AoxI GGCC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 365
AvaII GGWCC 1 cut(s) 365
BamHI GGATCC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 278
BbvCI CCTCAGC 1 cut(s) 12
BccI CCATC 1 cut(s) 71
BfaI CTAG 1 cut(s) 176
BfrI CTTAAG 1 cut(s) 190
BglII AGATCT 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 2 cut(s) 123, 366
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 314
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 132, 285
Bse1I ACTGG 3 cut(s) 248, 290, 455
BseGI GGATG 2 cut(s) 82, 483
BseLI CCNNNNNNNGG 3 cut(s) 131, 132, 285
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 3 cut(s) 248, 290, 455
BseYI CCCAGC 1 cut(s) 264
BshFI GGCC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 131, 132, 285
BsnI GGCC 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 6 cut(s) 84, 93, 121, 202, 318, 459
BspACI CCGC 1 cut(s) 72
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 123, 366
BspPI GGATC 3 cut(s) 116, 129, 467
BspTI CTTAAG 1 cut(s) 190
BsrI ACTGG 3 cut(s) 248, 290, 455
BssMI GATC 6 cut(s) 84, 93, 121, 202, 318, 459
BssNI GRCGYC 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 3 cut(s) 177, 368, 459
BstACI GRCGYC 1 cut(s) 314
BstAFI CTTAAG 1 cut(s) 190
BstC8I GCNNGC 2 cut(s) 182, 262
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 82, 483
BstKTI GATC 6 cut(s) 87, 96, 124, 205, 321, 462
BstMBI GATC 6 cut(s) 84, 93, 121, 202, 318, 459
BstMWI GCNNNNNNNGC 2 cut(s) 261, 303
BstX2I RGATCY 2 cut(s) 93, 121
BstYI RGATCY 2 cut(s) 93, 121
BsuRI GGCC 1 cut(s) 282
BtsCI GGATG 2 cut(s) 82, 483
BtsIMutI CAGTG 2 cut(s) 241, 283
Cac8I GCNNGC 2 cut(s) 182, 262
CaiI CAGNNNCTG 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 365
CseI GACGC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 328
CviJI RGCY 9 cut(s) 5, 59, 180, 240, 255, 264, 282, 297, 306
CviKI_1 RGCY 9 cut(s) 5, 59, 180, 240, 255, 264, 282, 297, 306
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 1 cut(s) 12
DpnI GATC 6 cut(s) 86, 95, 123, 204, 320, 461
DpnII GATC 6 cut(s) 84, 93, 121, 202, 318, 459
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 3 cut(s) 177, 368, 459
EarI CTCTTC 3 cut(s) 177, 368, 459
Eco47I GGWCC 1 cut(s) 365
FaiI YATR 5 cut(s) 56, 362, 435, 483, 485
FauI CCCGC 1 cut(s) 65
FokI GGATG 1 cut(s) 89
FspBI CTAG 1 cut(s) 176
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 314
HinfI GANTC 4 cut(s) 169, 289, 349, 397
HpaII CCGG 1 cut(s) 279
Hpy188III TCNNGA 2 cut(s) 148, 157
Hpy99I CGWCG 2 cut(s) 316, 328
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 2 cut(s) 184, 220
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 303
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 314
Kzo9I GATC 6 cut(s) 84, 93, 121, 202, 318, 459
LmnI GCTCC 1 cut(s) 281
LpnPI CCDG 7 cut(s) 206, 229, 250, 271, 292, 338, 436
LweI GCATC 1 cut(s) 36
MaeI CTAG 1 cut(s) 176
MaeII ACGT 1 cut(s) 143
MalI GATC 6 cut(s) 86, 95, 123, 204, 320, 461
MboI GATC 6 cut(s) 84, 93, 121, 202, 318, 459
MboII GAAGA 4 cut(s) 164, 385, 406, 476
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 2 cut(s) 93, 121
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 2 cut(s) 31, 402
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 4 cut(s) 21, 153, 164, 217
MseI TTAA 1 cut(s) 191
MspCI CTTAAG 1 cut(s) 190
MspI CCGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 402
MwoI GCNNNNNNNGC 2 cut(s) 261, 303
NdeII GATC 6 cut(s) 84, 93, 121, 202, 318, 459
NlaIV GGNNCC 2 cut(s) 123, 366
PcsI WCGNNNNNNNCGW 2 cut(s) 308, 320
PfeI GAWTC 3 cut(s) 289, 349, 397
PflFI GACNNNGTC 1 cut(s) 65
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 123, 366
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 243
PsuI RGATCY 2 cut(s) 93, 121
PsyI GACNNNGTC 1 cut(s) 65
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 191
Sau3AI GATC 6 cut(s) 84, 93, 121, 202, 318, 459
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 278
SetI ASST 5 cut(s) 69, 146, 156, 266, 308
SfaNI GCATC 1 cut(s) 36
SinI GGWCC 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 155, 190
SmoI CTYRAG 2 cut(s) 155, 190
Sse9I AATT 2 cut(s) 31, 402
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 1 cut(s) 146
TaqI TCGA 3 cut(s) 147, 167, 462
TasI AATT 2 cut(s) 31, 402
TfiI GAWTC 3 cut(s) 289, 349, 397
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 2 cut(s) 248, 290
TspDTI ATGAA 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 2 cut(s) 248, 290
Tth111I GACNNNGTC 1 cut(s) 65
Vha464I CTTAAG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 1 cut(s) 31
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.