AT1G12330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016715
Physical Location & Seq
Forward (+)
4194420 .. 4196749
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12330.1

Sequence Viewer

Length: 1518 bp
ATGGCTCCTTCGTCTTCATCTTCTCCTTCCAAATCTCCTGTTCCATCTCTTCACCAATCCCTTCACTTCACTCCTATTCTAGAATGTGAAGAAGACGACCTCCAAGAAGAAAGATACAAAAACAGAGCAACACCAAGCAGCGACGGTGGTTCTTCCGCCACACCAAACCACCAGCACCACCGCCGTACCAACAGCAACAACAACCACCAGCACACTCTAACACCTCTCCACCACAACGGCAAACCCCAAACCAGAAAACGACATGACGACAACGACGAAGACGATGGTGGTGCCGTATCCTGCAACAAGTGTCGACCTCACCACTCTCACCGTGACAAATTCTCCGTCGTACCTCTCGAAAGCCACAACAACCCTTCTTTCATCTCTAGCCCCAATCTCATAATCAAATCCATCTTCCAATCTCTCACACGGAGAAGCCCCAAACCTTCCTCTGCAACGGCGGCGCTTCCACCGCGCTCTTCCTCATCCTCCGCCGCAGATGCTTCTAGAGAAGAACAGTGGCGGTTAGCTGTGGCGGAGCTTTCTCATAAACTGATTCAAGCCACGAAGAAGAAAGAAGACGCGGTTATAGAAGCTTCTAGGTTGAAATCATCCATGGCAGAGCTAGAGAAGAAACTCAATAAACTCGAGATTTACTGTCACAATCTCAAATCAGGTCTCGACGAATGCAGCAACAAAAAACAGAGTGTTCCGATTCGAAAAGACGGATTCAACGACAGGATCATTCAGCAGTTTCTCGTCTCTGTGTCGGAATCTCGTTCTTCGATCAGAGCTTTAAGTAGATCTCTCGCCTCGCAGCTACGAACCGTCGGTGGCAAAGTTTACGAGAGACTCTCTCTTCTTCTTCAACCTTTCGATGTAAAGATCAACTCTTTTGCCAAAAACCCTAAAAGTCTGATCTTTTACCTCGAAGCGATTCTGAGCAGAGCCTTCTTCGAGGATTTCGAAGCTCCTGGGTTTCAGAAAAACGGGTCGACCCGGATTTTGAATCCAATTGATCGTTGTGAATCCAATTACGCTTCGTTTAACGTCTTGATGGAGCTGACGTGGGATGAGGTGTTGAGCAGAGGAACGAAGCATTTCTCGGAGGAGTTTAGCCGTTTCTGTGACCGGAAGATGAGTGACGTCGTTTCGATGCTTAGCTGGAACCGTGCCTGGCCTGAACCGCTTTTACAGGCTTTCTTTGGTGCGTCAAAGAGTGTTTGGTTGGTTCATCTTTTGGCTAATTCAGTGAACCCGGGGTTGCAAATCTTTCGTGTGGAGAAAGATGACCGGTTTGATCCGATTTACATGGAGGAAACCGGTGGGGAACGGTTTAAGAGTCTGGTTAGAGCTATGGTTCAGCCCGGGTTTTATGTCTATGGGAGTGTGGTTAAGTGCAAGGTGGTTTGCAAGCAATGCGGCAGCGATGAGGAAGAACTAGAAGACCATACGGTCAAAGAATGTAATAAAAACGACAAGAGTTTAATAAGCATTTGTAGCCCATTAGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

505

Amino Acids

56.82

Weight (kDa)

8.6

Isoelectric Point (pI)

62.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12330
fragaria_vesca FvH4_5g00760
malus_domestica MD04G1079100.v1.1
prunus_persica Prupe.5G080100_v2.0.a1
pyrus_communis pycom04g07090
rosa_chinensis RchiOBHm_Chr7g0201471
rosa_laevigata RLG00000003675
rosa_roxburghii Rroxscaffold_3G00255290
rosa_rugosa Rorug07G0063700
rosa_samantha Rh7AG190600 Rh7BG192800 Rh7CG201900 Rh7DG195400
rosa_wichuraiana Rw7G016720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1454
AatII GACGTC 1 cut(s) 1149
AccB1I GGYRCC 1 cut(s) 290
AccI GTMKAC 2 cut(s) 313, 995
AccII CGCG 2 cut(s) 475, 584
AclWI GGATC 2 cut(s) 749, 1295
AcsI RAATTY 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 1146
AfaI GTAC 2 cut(s) 187, 351
AfiI CCNNNNNNNGG 1 cut(s) 1511
AgeI ACCGGT 2 cut(s) 1293, 1322
AgsI TTSAA 5 cut(s) 560, 607, 733, 869, 1009
AjiI CACGTC 1 cut(s) 1068
AjnI CCWGG 2 cut(s) 973, 1175
Alw26I GTCTC 3 cut(s) 683, 766, 844
AlwI GGATC 2 cut(s) 749, 1295
Ama87I CYCGRG 3 cut(s) 647, 1258, 1367
AoxI GGCC 1 cut(s) 1178
ApeKI GCWGC 4 cut(s) 138, 690, 817, 1425
ApoI RAATTY 1 cut(s) 338
ArsI GACNNNNNNTTYG 4 cut(s) 330, 362, 988, 1020
AsiGI ACCGGT 2 cut(s) 1293, 1322
Asp700I GAANNNNTTC 2 cut(s) 936, 1100
AspLEI GCGC 2 cut(s) 466, 477
AsuC2I CCSGG 5 cut(s) 1000, 1259, 1260, 1368, 1369
AsuHPI GGTGA 3 cut(s) 44, 311, 320
AsuII TTCGAA 2 cut(s) 718, 966
AvaI CYCGRG 3 cut(s) 647, 1258, 1367
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BanI GGYRCC 1 cut(s) 290
BbsI GAAGAC 5 cut(s) 6, 99, 285, 585, 1452
BbvI GCAGC 4 cut(s) 150, 702, 829, 1437
BccI CCATC 4 cut(s) 52, 278, 419, 1051
BceAI ACGGC 5 cut(s) 168, 253, 278, 474, 1104
BcgI CGANNNNNNTGC 4 cut(s) 272, 306, 1256, 1290
BciT130I CCWGG 2 cut(s) 975, 1177
BciVI GTATCC 1 cut(s) 307
BcnI CCSGG 5 cut(s) 1000, 1259, 1260, 1368, 1369
BcoDI GTCTC 3 cut(s) 683, 766, 844
BfaI CTAG 6 cut(s) 80, 387, 507, 600, 626, 1442
BfoI RGCGCY 1 cut(s) 467
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 803
BisI GCNGC 7 cut(s) 139, 462, 495, 691, 818, 1423, 1426
BlpI GCTNAGC 1 cut(s) 1160
BlsI GCNGC 7 cut(s) 140, 463, 496, 692, 819, 1424, 1427
Bme1390I CCNGG 7 cut(s) 975, 1000, 1177, 1259, 1260, 1368, 1369
BmeT110I CYCGRG 3 cut(s) 647, 1258, 1367
BmgBI CACGTC 1 cut(s) 1068
BmiI GGNNCC 3 cut(s) 6, 292, 1169
BmrFI CCNGG 7 cut(s) 975, 1000, 1177, 1259, 1260, 1368, 1369
BmsI GCATC 2 cut(s) 490, 1146
BpiI GAAGAC 5 cut(s) 6, 99, 285, 585, 1452
BplI GAGNNNNNCTC 4 cut(s) 839, 871, 841, 873
Bpu1102I GCTNAGC 1 cut(s) 1160
Bpu14I TTCGAA 2 cut(s) 718, 966
BpuMI CCSGG 5 cut(s) 1000, 1259, 1260, 1368, 1369
BsaHI GRCGYC 1 cut(s) 1146
BsaI GGTCTC 1 cut(s) 683
BsaJI CCNNGG 5 cut(s) 615, 974, 1258, 1259, 1367
BsaWI WCCGGW 3 cut(s) 1131, 1293, 1322
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 1052, 1082
Bsc4I CCNNNNNNNGG 1 cut(s) 1511
Bse118I RCCGGY 2 cut(s) 1293, 1322
Bse3DI GCAATG 1 cut(s) 1424
BseBI CCWGG 2 cut(s) 975, 1177
BseDI CCNNGG 5 cut(s) 615, 974, 1258, 1259, 1367
BseGI GGATG 3 cut(s) 485, 611, 1078
BseLI CCNNNNNNNGG 1 cut(s) 1511
BseMI GCAATG 1 cut(s) 1424
BseMII CTCAG 1 cut(s) 932
BseRI GAGGAG 1 cut(s) 1124
BseXI GCAGC 4 cut(s) 150, 702, 829, 1437
Bsh1236I CGCG 2 cut(s) 475, 584
BshFI GGCC 1 cut(s) 1180
BshNI GGYRCC 1 cut(s) 290
BshTI ACCGGT 2 cut(s) 1293, 1322
BsiHKCI CYCGRG 3 cut(s) 647, 1258, 1367
BsiSI CCGG 6 cut(s) 1000, 1132, 1259, 1294, 1323, 1368
BslI CCNNNNNNNGG 1 cut(s) 1511
BsmAI GTCTC 3 cut(s) 683, 766, 844
BsmBI CGTCTC 1 cut(s) 766
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 1 cut(s) 1180
Bso31I GGTCTC 1 cut(s) 683
BsoBI CYCGRG 3 cut(s) 647, 1258, 1367
Bsp119I TTCGAA 2 cut(s) 718, 966
Bsp143I GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
Bsp1720I GCTNAGC 1 cut(s) 1160
Bsp19I CCATGG 1 cut(s) 615
BspANI GGCC 1 cut(s) 1180
BspCNI CTCAG 1 cut(s) 933
BspFNI CGCG 2 cut(s) 475, 584
BspLI GGNNCC 3 cut(s) 6, 292, 1169
BspPI GGATC 2 cut(s) 749, 1295
BspQI GCTCTTC 1 cut(s) 484
BspT104I TTCGAA 2 cut(s) 718, 966
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 683
BsrDI GCAATG 1 cut(s) 1424
BsrFI RCCGGY 2 cut(s) 1293, 1322
BssAI RCCGGY 2 cut(s) 1293, 1322
BssECI CCNNGG 5 cut(s) 615, 974, 1258, 1259, 1367
BssMI GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
BssNI GRCGYC 1 cut(s) 1146
BssT1I CCWWGG 1 cut(s) 615
Bst2UI CCWGG 2 cut(s) 975, 1177
Bst4CI ACNGT 8 cut(s) 146, 332, 519, 659, 829, 1172, 1335, 1456
Bst6I CTCTTC 3 cut(s) 54, 484, 864
BstACI GRCGYC 1 cut(s) 1146
BstAPI GCANNNNNTGC 1 cut(s) 1419
BstBI TTCGAA 2 cut(s) 718, 966
BstC8I GCNNGC 1 cut(s) 1415
BstDEI CTNAG 2 cut(s) 941, 1160
BstDSI CCRYGG 1 cut(s) 615
BstF5I GGATG 3 cut(s) 485, 611, 1078
BstFNI CGCG 2 cut(s) 475, 584
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 2 cut(s) 466, 477
BstKTI GATC 7 cut(s) 744, 789, 806, 888, 921, 1021, 1303
BstMAI GTCTC 3 cut(s) 683, 766, 844
BstMBI GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
BstMWI GCNNNNNNNGC 6 cut(s) 461, 472, 500, 1186, 1419, 1500
BstNI CCWGG 2 cut(s) 975, 1177
BstSCI CCNGG 7 cut(s) 973, 998, 1175, 1257, 1258, 1366, 1367
BstUI CGCG 2 cut(s) 475, 584
BstV1I GCAGC 4 cut(s) 150, 702, 829, 1437
BstV2I GAAGAC 5 cut(s) 6, 99, 285, 585, 1452
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 1 cut(s) 1180
BtgI CCRYGG 1 cut(s) 615
BtgZI GCGATG 1 cut(s) 1443
BtrI CACGTC 1 cut(s) 1068
BtsCI GGATG 3 cut(s) 485, 611, 1078
BtsIMutI CAGTG 2 cut(s) 524, 1257
Cac8I GCNNGC 1 cut(s) 1415
CfoI GCGC 2 cut(s) 466, 477
Cfr10I RCCGGY 2 cut(s) 1293, 1322
Cfr9I CCCGGG 2 cut(s) 1258, 1367
CseI GACGC 2 cut(s) 590, 1200
Csp6I GTAC 2 cut(s) 186, 350
CspAI ACCGGT 2 cut(s) 1293, 1322
CviAII CATG 3 cut(s) 263, 616, 1312
CviQI GTAC 2 cut(s) 186, 350
DdeI CTNAG 2 cut(s) 941, 1160
DpnI GATC 7 cut(s) 743, 788, 805, 887, 920, 1020, 1302
DpnII GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
DrdI GACNNNNNNGTC 1 cut(s) 1454
DseDI GACNNNNNNGTC 1 cut(s) 1454
Eam1104I CTCTTC 3 cut(s) 54, 484, 864
EarI CTCTTC 3 cut(s) 54, 484, 864
EciI GGCGGA 3 cut(s) 145, 481, 551
Eco130I CCWWGG 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 683
Eco88I CYCGRG 3 cut(s) 647, 1258, 1367
EcoRII CCWGG 2 cut(s) 973, 1175
EcoT14I CCWWGG 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 615
Esp3I CGTCTC 1 cut(s) 766
FaeI CATG 3 cut(s) 266, 619, 1315
FatI CATG 3 cut(s) 262, 615, 1311
FblI GTMKAC 2 cut(s) 313, 995
Fnu4HI GCNGC 7 cut(s) 139, 462, 495, 691, 818, 1423, 1426
FokI GGATG 3 cut(s) 472, 598, 1085
Fsp4HI GCNGC 7 cut(s) 139, 462, 495, 691, 818, 1423, 1426
FspBI CTAG 6 cut(s) 80, 387, 507, 600, 626, 1442
GlaI GCGC 2 cut(s) 465, 476
GluI GCNGC 7 cut(s) 139, 462, 495, 691, 818, 1423, 1426
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 1 cut(s) 1180
HapII CCGG 6 cut(s) 1000, 1132, 1259, 1294, 1323, 1368
HgaI GACGC 2 cut(s) 590, 1200
HhaI GCGC 2 cut(s) 466, 477
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 3 cut(s) 266, 619, 1315
Hin6I GCGC 2 cut(s) 464, 475
HinP1I GCGC 2 cut(s) 464, 475
HincII GTYRAC 2 cut(s) 314, 996
HindII GTYRAC 2 cut(s) 314, 996
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 9 cut(s) 556, 715, 729, 773, 852, 937, 1009, 1028, 1342
HpaII CCGG 6 cut(s) 1000, 1132, 1259, 1294, 1323, 1368
HphI GGTGA 3 cut(s) 44, 311, 320
Hpy166II GTNNAC 4 cut(s) 314, 844, 996, 1255
Hpy188I TCNGA 8 cut(s) 714, 772, 791, 918, 942, 984, 1108, 1305
Hpy188III TCNNGA 6 cut(s) 80, 356, 507, 649, 680, 1054
Hpy8I GTNNAC 4 cut(s) 314, 844, 996, 1255
Hpy99I CGWCG 6 cut(s) 146, 278, 350, 686, 833, 1151
HpyAV CCTTC 6 cut(s) 18, 36, 71, 384, 456, 961
HpyCH4III ACNGT 8 cut(s) 146, 332, 519, 659, 829, 1172, 1335, 1456
HpyCH4IV ACGT 3 cut(s) 1050, 1067, 1146
HpyCH4V TGCA 6 cut(s) 303, 455, 690, 1267, 1401, 1413
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 472, 500, 1186, 1419, 1500
HpyF3I CTNAG 2 cut(s) 941, 1160
HpySE526I ACGT 3 cut(s) 1050, 1067, 1146
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 3 cut(s) 266, 619, 1315
HspAI GCGC 2 cut(s) 464, 475
Kzo9I GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
LguI GCTCTTC 1 cut(s) 484
LmnI GCTCC 4 cut(s) 10, 538, 976, 1060
Lsp1109I GCAGC 4 cut(s) 150, 702, 829, 1437
LweI GCATC 2 cut(s) 490, 1146
MaeI CTAG 6 cut(s) 80, 387, 507, 600, 626, 1442
MaeII ACGT 3 cut(s) 1050, 1067, 1146
MaeIII GTNAC 4 cut(s) 332, 659, 1127, 1142
MalI GATC 7 cut(s) 743, 788, 805, 887, 920, 1020, 1302
MboI GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 1 cut(s) 803
MluCI AATT 4 cut(s) 338, 1014, 1033, 1246
MlyI GAGTC 2 cut(s) 846, 1351
MmeI TCCRAC 1 cut(s) 750
MroXI GAANNNNTTC 2 cut(s) 936, 1100
MseI TTAA 6 cut(s) 797, 1047, 1338, 1395, 1487, 1516
MspI CCGG 6 cut(s) 1000, 1132, 1259, 1294, 1323, 1368
MspR9I CCNGG 7 cut(s) 975, 1000, 1177, 1259, 1260, 1368, 1369
MunI CAATTG 1 cut(s) 1014
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 2 cut(s) 975, 1177
MvnI CGCG 2 cut(s) 475, 584
MwoI GCNNNNNNNGC 6 cut(s) 461, 472, 500, 1186, 1419, 1500
NciI CCSGG 5 cut(s) 1000, 1259, 1260, 1368, 1369
NcoI CCATGG 1 cut(s) 615
NdeII GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
NlaIII CATG 3 cut(s) 266, 619, 1315
NlaIV GGNNCC 3 cut(s) 6, 292, 1169
NmuCI GTSAC 4 cut(s) 332, 659, 1127, 1142
NspV TTCGAA 2 cut(s) 718, 966
PaeR7I CTCGAG 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 484
PcsI WCGNNNNNNNCGW 5 cut(s) 273, 279, 354, 732, 963
PctI GAATGC 1 cut(s) 692
PdmI GAANNNNTTC 2 cut(s) 936, 1100
PfeI GAWTC 7 cut(s) 556, 715, 729, 773, 937, 1009, 1028
PinAI ACCGGT 2 cut(s) 1293, 1322
PkrI GCNGC 7 cut(s) 140, 463, 496, 692, 819, 1424, 1427
PleI GAGTC 2 cut(s) 846, 1350
PpsI GAGTC 2 cut(s) 846, 1350
Psp6I CCWGG 2 cut(s) 973, 1175
PspGI CCWGG 2 cut(s) 973, 1175
PspN4I GGNNCC 3 cut(s) 6, 292, 1169
PsrI GAACNNNNNNTAC 2 cut(s) 1176, 1208
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 2 cut(s) 187, 351
RsaNI GTAC 2 cut(s) 186, 350
SalI GTCGAC 2 cut(s) 312, 994
SapI GCTCTTC 1 cut(s) 484
SaqAI TTAA 6 cut(s) 797, 1047, 1338, 1395, 1487, 1516
SatI GCNGC 7 cut(s) 139, 462, 495, 691, 818, 1423, 1426
Sau3AI GATC 7 cut(s) 741, 786, 803, 885, 918, 1018, 1300
SchI GAGTC 2 cut(s) 846, 1351
ScrFI CCNGG 7 cut(s) 975, 1000, 1177, 1259, 1260, 1368, 1369
SfaNI GCATC 2 cut(s) 490, 1146
Sfr274I CTCGAG 1 cut(s) 647
SfuI TTCGAA 2 cut(s) 718, 966
SlaI CTCGAG 1 cut(s) 647
SmaI CCCGGG 2 cut(s) 1260, 1369
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
Sse9I AATT 4 cut(s) 338, 1014, 1033, 1246
SspMI CTAG 6 cut(s) 80, 387, 507, 600, 626, 1442
StyD4I CCNGG 7 cut(s) 973, 998, 1175, 1257, 1258, 1366, 1367
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 8 cut(s) 146, 332, 519, 659, 829, 1172, 1335, 1456
TaiI ACGT 3 cut(s) 1053, 1070, 1149
TasI AATT 4 cut(s) 338, 1014, 1033, 1246
TauI GCSGC 3 cut(s) 464, 497, 1425
TfiI GAWTC 7 cut(s) 556, 715, 729, 773, 937, 1009, 1028
Tru1I TTAA 6 cut(s) 797, 1047, 1338, 1395, 1487, 1516
Tru9I TTAA 6 cut(s) 797, 1047, 1338, 1395, 1487, 1516
TscAI CASTG 2 cut(s) 524, 1257
TseFI GTSAC 4 cut(s) 332, 659, 1127, 1142
TseI GCWGC 4 cut(s) 138, 690, 817, 1425
Tsp45I GTSAC 4 cut(s) 332, 659, 1127, 1142
TspDTI ATGAA 3 cut(s) 6, 370, 1223
TspGWI ACGGA 3 cut(s) 334, 445, 741
TspMI CCCGGG 2 cut(s) 1258, 1367
TspRI CASTG 2 cut(s) 524, 1257
XapI RAATTY 1 cut(s) 338
XbaI TCTAGA 2 cut(s) 79, 506
XhoI CTCGAG 1 cut(s) 647
XmaI CCCGGG 2 cut(s) 1258, 1367
XmiI GTMKAC 2 cut(s) 313, 995
XmnI GAANNNNTTC 2 cut(s) 936, 1100
XspI CTAG 6 cut(s) 80, 387, 507, 600, 626, 1442
ZraI GACGTC 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.