AT1G55060

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0021843
Physical Location & Seq
Forward (+)
20549533 .. 20550225
693 bp
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UTR
Exon/CDS
Intron
AT1G55060.1

Sequence Viewer

Length: 693 bp
ATGCAGATTTTTCTCAAGACTCTTACCGGAAAGACGAAAGTTTTGGAAGTTGAGAGCTCGGACACAATCGATAACGTGAAGGCCAAGATTCAAGATATAGAAGGGATCCCACCAGACCAACATAGACTTATCTTTGCAGGCAAGCAGCTCGAAGATGGTCGGACTCTTGCTGATTACAACGTCCAGGAGGATTCCACCCTTCATCTCCTTCTGAGATTCCGTGGTGGGATGCAGATTTTCGTGAAGACCCTTACCGGCAAAACCATTACCCTAGAAGTTGAGAGCTCTGATACAATCGATAACCTGAAGGCCAAGATTCAGGACAAAGAAGGGATCCCACCAGACCAACAGAGACTCATCTTTGCAGGAAAGCAGCTCGAAGATGGTCGGACTCTTGCAGATTACAACATCCAAAAGGAGTCAACACTTCACCTTGTGCTGCGTCTTCGTGGTGGGATGCAGATTTTCGTAAAGACTTTGACGGGAAAGACCATCACGCTTGAAGTGGAAAGCTCTGATACGATTGACAATGTGAAGGCAAAGATTCAGGACAAAGAAGGGATTTCACCAGACCAACAGAGACTCATCTTTGCAGGCAAGCAGCATGAAGATGGTCGCACTTTAGCGGATTACAACATCCAGAAAGAGTCAACTCTTCACCTTGTTCTCCGTCTCCGCGGTGGAAGCTTCTGA

Protein Analysis

230

Amino Acids

25.84

Weight (kDa)

6.24

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 2.1e-13 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 73 5.4e-27 Ubiquitin family
Rad60-SLD PF11976 77 - 147 1.1e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 2.3e-32 Ubiquitin family
Rad60-SLD PF11976 153 - 223 7.3e-15 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1e-28 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55060
malus_domestica MD07G1147300.v1.1
prunus_persica Prupe.4G203900_v2.0.a1
rosa_wichuraiana Rw5G003270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 678
AciI CCGC 3 cut(s) 626, 676, 678
AclWI GGATC 4 cut(s) 100, 113, 328, 341
AcuI CTGAAG 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 436
AgsI TTSAA 2 cut(s) 92, 503
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 6 cut(s) 57, 148, 285, 376, 513, 687
AluI AGCT 6 cut(s) 57, 148, 285, 376, 513, 687
Alw21I GWGCWC 2 cut(s) 59, 287
Alw26I GTCTC 3 cut(s) 346, 574, 677
AlwI GGATC 4 cut(s) 100, 113, 328, 341
AoxI GGCC 2 cut(s) 81, 309
ApeKI GCWGC 4 cut(s) 145, 373, 439, 601
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
AsuHPI GGTGA 3 cut(s) 422, 558, 650
BamHI GGATCC 2 cut(s) 105, 333
BanII GRGCYC 2 cut(s) 59, 287
BbsI GAAGAC 2 cut(s) 251, 437
Bbv12I GWGCWC 2 cut(s) 59, 287
BbvI GCAGC 4 cut(s) 157, 385, 426, 613
BccI CCATC 4 cut(s) 149, 377, 500, 605
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 3 cut(s) 346, 574, 677
BfaI CTAG 1 cut(s) 272
BisI GCNGC 4 cut(s) 146, 374, 440, 602
BlsI GCNGC 4 cut(s) 147, 375, 441, 603
Bme1390I CCNGG 1 cut(s) 185
BmiI GGNNCC 2 cut(s) 107, 335
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 219, 447
BpiI GAAGAC 2 cut(s) 251, 437
Bsa29I ATCGAT 2 cut(s) 69, 297
BsaJI CCNNGG 2 cut(s) 220, 676
BsaWI WCCGGW 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 254
BseBI CCWGG 1 cut(s) 185
BseCI ATCGAT 2 cut(s) 69, 297
BseDI CCNNGG 2 cut(s) 220, 676
BseGI GGATG 4 cut(s) 234, 408, 462, 636
BseMII CTCAG 1 cut(s) 203
BseXI GCAGC 4 cut(s) 157, 385, 426, 613
Bsh1236I CGCG 1 cut(s) 678
BshFI GGCC 2 cut(s) 83, 311
BshVI ATCGAT 2 cut(s) 69, 297
BsiHKAI GWGCWC 2 cut(s) 59, 287
BsiSI CCGG 2 cut(s) 27, 255
BsmAI GTCTC 3 cut(s) 346, 574, 677
BsmBI CGTCTC 1 cut(s) 677
BsnI GGCC 2 cut(s) 83, 311
Bsp1286I GDGCHC 2 cut(s) 59, 287
Bsp143I GATC 2 cut(s) 105, 333
BspACI CCGC 3 cut(s) 626, 676, 678
BspANI GGCC 2 cut(s) 83, 311
BspCNI CTCAG 1 cut(s) 204
BspDI ATCGAT 2 cut(s) 69, 297
BspFNI CGCG 1 cut(s) 678
BspLI GGNNCC 2 cut(s) 107, 335
BspPI GGATC 4 cut(s) 100, 113, 328, 341
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 220, 676
BssMI GATC 2 cut(s) 105, 333
Bst2UI CCWGG 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 660
BstC8I GCNNGC 4 cut(s) 139, 143, 595, 599
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 2 cut(s) 220, 676
BstF5I GGATG 4 cut(s) 234, 408, 462, 636
BstFNI CGCG 1 cut(s) 678
BstKTI GATC 2 cut(s) 108, 336
BstMAI GTCTC 3 cut(s) 346, 574, 677
BstMBI GATC 2 cut(s) 105, 333
BstMWI GCNNNNNNNGC 1 cut(s) 684
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstUI CGCG 1 cut(s) 678
BstV1I GCAGC 4 cut(s) 157, 385, 426, 613
BstV2I GAAGAC 2 cut(s) 251, 437
BstX2I RGATCY 2 cut(s) 105, 333
BstYI RGATCY 2 cut(s) 105, 333
Bsu15I ATCGAT 2 cut(s) 69, 297
BsuRI GGCC 2 cut(s) 83, 311
BsuTUI ATCGAT 2 cut(s) 69, 297
BtgI CCRYGG 2 cut(s) 220, 676
BtsCI GGATG 4 cut(s) 234, 408, 462, 636
Cac8I GCNNGC 4 cut(s) 139, 143, 595, 599
Cfr10I RCCGGY 1 cut(s) 254
Cfr42I CCGCGG 1 cut(s) 679
ClaI ATCGAT 2 cut(s) 69, 297
CseI GACGC 1 cut(s) 431
CviAII CATG 1 cut(s) 605
CviJI RGCY 8 cut(s) 57, 83, 148, 285, 311, 376, 513, 687
CviKI_1 RGCY 8 cut(s) 57, 83, 148, 285, 311, 376, 513, 687
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 107, 335
DpnII GATC 2 cut(s) 105, 333
DraIII CACNNNGTG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Ecl136II GAGCTC 2 cut(s) 57, 285
Eco24I GRGCYC 2 cut(s) 59, 287
Eco53kI GAGCTC 2 cut(s) 57, 285
Eco57I CTGAAG 1 cut(s) 326
EcoICRI GAGCTC 2 cut(s) 57, 285
EcoRII CCWGG 1 cut(s) 183
EcoT38I GRGCYC 2 cut(s) 59, 287
Esp3I CGTCTC 1 cut(s) 677
FaeI CATG 1 cut(s) 608
FaiI YATR 3 cut(s) 98, 123, 606
FatI CATG 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 146, 374, 440, 602
FokI GGATG 4 cut(s) 241, 395, 469, 623
FriOI GRGCYC 2 cut(s) 59, 287
Fsp4HI GCNGC 4 cut(s) 146, 374, 440, 602
FspBI CTAG 1 cut(s) 272
GluI GCNGC 4 cut(s) 146, 374, 440, 602
HaeIII GGCC 2 cut(s) 83, 311
HapII CCGG 2 cut(s) 27, 255
HgaI GACGC 1 cut(s) 431
Hin1II CATG 1 cut(s) 608
HincII GTYRAC 2 cut(s) 423, 651
HindII GTYRAC 2 cut(s) 423, 651
HindIII AAGCTT 1 cut(s) 685
HpaII CCGG 2 cut(s) 27, 255
HphI GGTGA 3 cut(s) 422, 558, 650
Hpy166II GTNNAC 2 cut(s) 423, 651
Hpy188I TCNGA 7 cut(s) 61, 162, 213, 289, 390, 517, 692
Hpy188III TCNNGA 6 cut(s) 16, 92, 241, 320, 548, 640
Hpy8I GTNNAC 2 cut(s) 423, 651
HpyAV CCTTC 8 cut(s) 73, 95, 209, 218, 301, 323, 529, 551
HpyCH4IV ACGT 2 cut(s) 75, 180
HpyCH4V TGCA 7 cut(s) 4, 137, 232, 365, 398, 460, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 684
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 2 cut(s) 75, 180
Hsp92II CATG 1 cut(s) 608
KspI CCGCGG 1 cut(s) 679
Kzo9I GATC 2 cut(s) 105, 333
Lsp1109I GCAGC 4 cut(s) 157, 385, 426, 613
LweI GCATC 2 cut(s) 219, 447
MaeI CTAG 1 cut(s) 272
MaeII ACGT 2 cut(s) 75, 180
MalI GATC 2 cut(s) 107, 335
MboI GATC 2 cut(s) 105, 333
MboII GAAGA 6 cut(s) 164, 256, 392, 437, 620, 647
MflI RGATCY 2 cut(s) 105, 333
MhlI GDGCHC 2 cut(s) 59, 287
MlyI GAGTC 7 cut(s) 13, 157, 348, 385, 428, 576, 656
MmeI TCCRAC 2 cut(s) 140, 368
MnlI CCTC 1 cut(s) 181
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 678
MspI CCGG 2 cut(s) 27, 255
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 678
MwoI GCNNNNNNNGC 1 cut(s) 684
NdeII GATC 2 cut(s) 105, 333
NlaIII CATG 1 cut(s) 608
NlaIV GGNNCC 2 cut(s) 107, 335
PfeI GAWTC 5 cut(s) 88, 191, 216, 316, 544
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 4 cut(s) 147, 375, 441, 603
PleI GAGTC 7 cut(s) 13, 157, 348, 385, 427, 576, 655
PpsI GAGTC 7 cut(s) 13, 157, 348, 385, 427, 576, 655
Psp124BI GAGCTC 2 cut(s) 59, 287
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 107, 335
PsuI RGATCY 2 cut(s) 105, 333
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 2 cut(s) 59, 287
SacII CCGCGG 1 cut(s) 679
SatI GCNGC 4 cut(s) 146, 374, 440, 602
Sau3AI GATC 2 cut(s) 105, 333
SchI GAGTC 7 cut(s) 13, 157, 348, 385, 428, 576, 656
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 59, 287
SfaNI GCATC 2 cut(s) 219, 447
Sfr303I CCGCGG 1 cut(s) 679
SgrBI CCGCGG 1 cut(s) 679
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SsiI CCGC 3 cut(s) 626, 676, 678
SspMI CTAG 1 cut(s) 272
SstI GAGCTC 2 cut(s) 59, 287
StyD4I CCNGG 1 cut(s) 183
TaiI ACGT 2 cut(s) 78, 183
TaqI TCGA 4 cut(s) 69, 150, 297, 378
TfiI GAWTC 5 cut(s) 88, 191, 216, 316, 544
TseI GCWGC 4 cut(s) 145, 373, 439, 601
TspDTI ATGAA 2 cut(s) 191, 621
TspGWI ACGGA 2 cut(s) 209, 659
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.