Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55060 |
| malus_domestica | MD07G1147300.v1.1 |
| prunus_persica | Prupe.4G203900_v2.0.a1 |
| rosa_wichuraiana | Rw5G003270 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 44 |
| AciI | CCGC | 1 cut(s) | 160 |
| AclWI | GGATC | 3 cut(s) | 82, 310, 538 |
| AcoI | YGGCCR | 1 cut(s) | 157 |
| AdeI | CACNNNGTG | 3 cut(s) | 208, 436, 664 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 97, 205, 255, 325, 433, 483, 553, 661 |
| AgsI | TTSAA | 5 cut(s) | 53, 281, 509, 698, 751 |
| AjnI | CCWGG | 3 cut(s) | 90, 318, 546 |
| AluBI | AGCT | 7 cut(s) | 57, 148, 285, 376, 513, 604, 702 |
| AluI | AGCT | 7 cut(s) | 57, 148, 285, 376, 513, 604, 702 |
| Alw21I | GWGCWC | 1 cut(s) | 669 |
| Alw26I | GTCTC | 1 cut(s) | 221 |
| AlwI | GGATC | 3 cut(s) | 82, 310, 538 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 127 |
| Ama87I | CYCGRG | 2 cut(s) | 44, 377 |
| AoxI | GGCC | 1 cut(s) | 157 |
| ApeKI | GCWGC | 3 cut(s) | 145, 373, 601 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 105 |
| AsuC2I | CCSGG | 1 cut(s) | 256 |
| AsuHPI | GGTGA | 3 cut(s) | 31, 259, 487 |
| AvaI | CYCGRG | 2 cut(s) | 44, 377 |
| BbsI | GAAGAC | 2 cut(s) | 266, 494 |
| Bbv12I | GWGCWC | 1 cut(s) | 669 |
| BbvI | GCAGC | 3 cut(s) | 157, 385, 613 |
| BccI | CCATC | 4 cut(s) | 44, 149, 377, 605 |
| BciT130I | CCWGG | 3 cut(s) | 92, 320, 548 |
| BciVI | GTATCC | 2 cut(s) | 283, 511 |
| BcnI | CCSGG | 1 cut(s) | 256 |
| BcoDI | GTCTC | 1 cut(s) | 221 |
| BfuI | GTATCC | 2 cut(s) | 283, 511 |
| BisI | GCNGC | 4 cut(s) | 146, 160, 374, 602 |
| BlsI | GCNGC | 4 cut(s) | 147, 161, 375, 603 |
| Bme1390I | CCNGG | 4 cut(s) | 92, 256, 320, 548 |
| BmeT110I | CYCGRG | 2 cut(s) | 44, 377 |
| BmrFI | CCNGG | 4 cut(s) | 92, 256, 320, 548 |
| BmrI | ACTGGG | 1 cut(s) | 492 |
| BmuI | ACTGGG | 1 cut(s) | 492 |
| BpiI | GAAGAC | 2 cut(s) | 266, 494 |
| BpuEI | CTTGAG | 2 cut(s) | 170, 626 |
| BpuMI | CCSGG | 1 cut(s) | 256 |
| BsaAI | YACGTR | 1 cut(s) | 677 |
| BsaJI | CCNNGG | 4 cut(s) | 220, 270, 448, 498 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 97, 205, 255, 325, 433, 483, 553, 661 |
| Bse1I | ACTGG | 1 cut(s) | 487 |
| BseBI | CCWGG | 3 cut(s) | 92, 320, 548 |
| BseDI | CCNNGG | 4 cut(s) | 220, 270, 448, 498 |
| BseGI | GGATG | 6 cut(s) | 160, 180, 388, 408, 616, 636 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 97, 205, 255, 325, 433, 483, 553, 661 |
| BseNI | ACTGG | 1 cut(s) | 487 |
| BseXI | GCAGC | 3 cut(s) | 157, 385, 613 |
| BshFI | GGCC | 1 cut(s) | 159 |
| BsiHKAI | GWGCWC | 1 cut(s) | 669 |
| BsiHKCI | CYCGRG | 2 cut(s) | 44, 377 |
| BsiSI | CCGG | 1 cut(s) | 255 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 97, 205, 255, 325, 433, 483, 553, 661 |
| BsmAI | GTCTC | 1 cut(s) | 221 |
| BsmBI | CGTCTC | 1 cut(s) | 221 |
| BsmI | GAATGC | 3 cut(s) | 105, 333, 561 |
| BsnI | GGCC | 1 cut(s) | 159 |
| BsoBI | CYCGRG | 2 cut(s) | 44, 377 |
| Bsp1286I | GDGCHC | 1 cut(s) | 669 |
| Bsp143I | GATC | 4 cut(s) | 87, 129, 315, 543 |
| BspACI | CCGC | 1 cut(s) | 160 |
| BspANI | GGCC | 1 cut(s) | 159 |
| BspPI | GGATC | 3 cut(s) | 82, 310, 538 |
| BsrI | ACTGG | 1 cut(s) | 487 |
| BssECI | CCNNGG | 4 cut(s) | 220, 270, 448, 498 |
| BssMI | GATC | 4 cut(s) | 87, 129, 315, 543 |
| BssT1I | CCWWGG | 2 cut(s) | 270, 498 |
| Bst2UI | CCWGG | 3 cut(s) | 92, 320, 548 |
| BstBAI | YACGTR | 1 cut(s) | 677 |
| BstC8I | GCNNGC | 4 cut(s) | 139, 143, 595, 599 |
| BstDSI | CCRYGG | 2 cut(s) | 220, 448 |
| BstF5I | GGATG | 6 cut(s) | 160, 180, 388, 408, 616, 636 |
| BstKTI | GATC | 4 cut(s) | 90, 132, 318, 546 |
| BstMAI | GTCTC | 1 cut(s) | 221 |
| BstMBI | GATC | 4 cut(s) | 87, 129, 315, 543 |
| BstNI | CCWGG | 3 cut(s) | 92, 320, 548 |
| BstSCI | CCNGG | 4 cut(s) | 90, 254, 318, 546 |
| BstV1I | GCAGC | 3 cut(s) | 157, 385, 613 |
| BstV2I | GAAGAC | 2 cut(s) | 266, 494 |
| BstX2I | RGATCY | 3 cut(s) | 87, 315, 543 |
| BstYI | RGATCY | 3 cut(s) | 87, 315, 543 |
| BsuI | GTATCC | 2 cut(s) | 283, 511 |
| BsuRI | GGCC | 1 cut(s) | 159 |
| BtgI | CCRYGG | 2 cut(s) | 220, 448 |
| BtsCI | GGATG | 6 cut(s) | 160, 180, 388, 408, 616, 636 |
| Cac8I | GCNNGC | 4 cut(s) | 139, 143, 595, 599 |
| CaiI | CAGNNNCTG | 1 cut(s) | 127 |
| DpnI | GATC | 4 cut(s) | 89, 131, 317, 545 |
| DpnII | GATC | 4 cut(s) | 87, 129, 315, 543 |
| DraIII | CACNNNGTG | 3 cut(s) | 208, 436, 664 |
| EaeI | YGGCCR | 1 cut(s) | 157 |
| Eco130I | CCWWGG | 2 cut(s) | 270, 498 |
| Eco88I | CYCGRG | 2 cut(s) | 44, 377 |
| EcoRII | CCWGG | 3 cut(s) | 90, 318, 546 |
| EcoT14I | CCWWGG | 2 cut(s) | 270, 498 |
| ErhI | CCWWGG | 2 cut(s) | 270, 498 |
| Esp3I | CGTCTC | 1 cut(s) | 221 |
| FaiI | YATR | 2 cut(s) | 230, 458 |
| Fnu4HI | GCNGC | 4 cut(s) | 146, 160, 374, 602 |
| FokI | GGATG | 6 cut(s) | 167, 167, 395, 395, 623, 623 |
| Fsp4HI | GCNGC | 4 cut(s) | 146, 160, 374, 602 |
| GluI | GCNGC | 4 cut(s) | 146, 160, 374, 602 |
| HaeIII | GGCC | 1 cut(s) | 159 |
| HapII | CCGG | 1 cut(s) | 255 |
| HinfI | GANTC | 5 cut(s) | 191, 419, 444, 647, 763 |
| HpaII | CCGG | 1 cut(s) | 255 |
| HphI | GGTGA | 3 cut(s) | 31, 259, 487 |
| Hpy166II | GTNNAC | 3 cut(s) | 195, 423, 651 |
| Hpy188I | TCNGA | 5 cut(s) | 61, 289, 517, 671, 706 |
| Hpy188III | TCNNGA | 6 cut(s) | 13, 184, 244, 412, 472, 640 |
| Hpy8I | GTNNAC | 3 cut(s) | 195, 423, 651 |
| HpyAV | CCTTC | 6 cut(s) | 95, 181, 409, 437, 637, 665 |
| HpyCH4IV | ACGT | 1 cut(s) | 676 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 232, 460 |
| HpySE526I | ACGT | 1 cut(s) | 676 |
| Kzo9I | GATC | 4 cut(s) | 87, 129, 315, 543 |
| LmnI | GCTCC | 1 cut(s) | 672 |
| Lsp1109I | GCAGC | 3 cut(s) | 157, 385, 613 |
| MaeII | ACGT | 1 cut(s) | 676 |
| MalI | GATC | 4 cut(s) | 89, 131, 317, 545 |
| MboI | GATC | 4 cut(s) | 87, 129, 315, 543 |
| MboII | GAAGA | 3 cut(s) | 271, 499, 763 |
| MflI | RGATCY | 3 cut(s) | 87, 315, 543 |
| MhlI | GDGCHC | 1 cut(s) | 669 |
| MlyI | GAGTC | 4 cut(s) | 200, 428, 438, 656 |
| MmeI | TCCRAC | 1 cut(s) | 694 |
| MroXI | GAANNNNTTC | 1 cut(s) | 105 |
| MspI | CCGG | 1 cut(s) | 255 |
| MspR9I | CCNGG | 4 cut(s) | 92, 256, 320, 548 |
| Mva1269I | GAATGC | 3 cut(s) | 105, 333, 561 |
| MvaI | CCWGG | 3 cut(s) | 92, 320, 548 |
| NciI | CCSGG | 1 cut(s) | 256 |
| NdeII | GATC | 4 cut(s) | 87, 129, 315, 543 |
| PaeR7I | CTCGAG | 2 cut(s) | 44, 377 |
| PctI | GAATGC | 3 cut(s) | 105, 333, 561 |
| PdmI | GAANNNNTTC | 1 cut(s) | 105 |
| PfeI | GAWTC | 1 cut(s) | 763 |
| PflFI | GACNNNGTC | 3 cut(s) | 74, 302, 530 |
| PfoI | TCCNGGA | 3 cut(s) | 90, 318, 546 |
| PkrI | GCNGC | 4 cut(s) | 147, 161, 375, 603 |
| PleI | GAGTC | 4 cut(s) | 199, 427, 438, 655 |
| PpsI | GAGTC | 4 cut(s) | 199, 427, 438, 655 |
| Ppu21I | YACGTR | 1 cut(s) | 677 |
| Psp6I | CCWGG | 3 cut(s) | 90, 318, 546 |
| PspGI | CCWGG | 3 cut(s) | 90, 318, 546 |
| PspXI | VCTCGAGB | 2 cut(s) | 44, 377 |
| PstNI | CAGNNNCTG | 1 cut(s) | 127 |
| PsuI | RGATCY | 3 cut(s) | 87, 315, 543 |
| PsyI | GACNNNGTC | 3 cut(s) | 74, 302, 530 |
| SatI | GCNGC | 4 cut(s) | 146, 160, 374, 602 |
| Sau3AI | GATC | 4 cut(s) | 87, 129, 315, 543 |
| SchI | GAGTC | 4 cut(s) | 200, 428, 438, 656 |
| ScrFI | CCNGG | 4 cut(s) | 92, 256, 320, 548 |
| SduI | GDGCHC | 1 cut(s) | 669 |
| Sfr274I | CTCGAG | 2 cut(s) | 44, 377 |
| SlaI | CTCGAG | 2 cut(s) | 44, 377 |
| SmlI | CTYRAG | 4 cut(s) | 44, 149, 377, 605 |
| SmoI | CTYRAG | 4 cut(s) | 44, 149, 377, 605 |
| SsiI | CCGC | 1 cut(s) | 160 |
| StyD4I | CCNGG | 4 cut(s) | 90, 254, 318, 546 |
| StyI | CCWWGG | 2 cut(s) | 270, 498 |
| TaiI | ACGT | 1 cut(s) | 679 |
| TaqI | TCGA | 3 cut(s) | 45, 378, 442 |
| TauI | GCSGC | 1 cut(s) | 162 |
| TfiI | GAWTC | 1 cut(s) | 763 |
| TseI | GCWGC | 3 cut(s) | 145, 373, 601 |
| TspGWI | ACGGA | 2 cut(s) | 209, 437 |
| Tth111I | GACNNNGTC | 3 cut(s) | 74, 302, 530 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 202, 430, 658 |
| XhoI | CTCGAG | 2 cut(s) | 44, 377 |
| XmnI | GAANNNNTTC | 1 cut(s) | 105 |