Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55060 |
| malus_domestica | MD07G1147300.v1.1 |
| prunus_persica | Prupe.4G203900_v2.0.a1 |
| rosa_wichuraiana | Rw5G003270 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 137 |
| AciI | CCGC | 2 cut(s) | 110, 219 |
| AclWI | GGATC | 2 cut(s) | 82, 310 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 325 |
| AgsI | TTSAA | 1 cut(s) | 47 |
| AluBI | AGCT | 1 cut(s) | 285 |
| AluI | AGCT | 1 cut(s) | 285 |
| AlwI | GGATC | 2 cut(s) | 82, 310 |
| Aor13HI | TCCGGA | 1 cut(s) | 137 |
| BbsI | GAAGAC | 1 cut(s) | 251 |
| BccI | CCATC | 2 cut(s) | 95, 149 |
| BclI | TGATCA | 1 cut(s) | 343 |
| BfmI | CTRYAG | 2 cut(s) | 66, 294 |
| BglII | AGATCT | 2 cut(s) | 6, 54 |
| BmsI | GCATC | 2 cut(s) | 211, 218 |
| BpiI | GAAGAC | 1 cut(s) | 251 |
| BpuEI | CTTGAG | 1 cut(s) | 293 |
| BsaBI | GATNNNNATC | 1 cut(s) | 232 |
| BsaWI | WCCGGW | 1 cut(s) | 137 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 325 |
| Bse1I | ACTGG | 1 cut(s) | 259 |
| Bse8I | GATNNNNATC | 1 cut(s) | 232 |
| BseAI | TCCGGA | 1 cut(s) | 137 |
| BseGI | GGATG | 1 cut(s) | 233 |
| BseJI | GATNNNNATC | 1 cut(s) | 232 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 325 |
| BseNI | ACTGG | 1 cut(s) | 259 |
| BsiSI | CCGG | 1 cut(s) | 138 |
| BslFI | GGGAC | 1 cut(s) | 118 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 325 |
| BsmFI | GGGAC | 1 cut(s) | 118 |
| Bsp13I | TCCGGA | 1 cut(s) | 137 |
| Bsp143I | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| BspACI | CCGC | 2 cut(s) | 110, 219 |
| BspEI | TCCGGA | 1 cut(s) | 137 |
| BspPI | GGATC | 2 cut(s) | 82, 310 |
| BsrI | ACTGG | 1 cut(s) | 259 |
| BssMI | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| Bst4CI | ACNGT | 1 cut(s) | 265 |
| BstF5I | GGATG | 1 cut(s) | 233 |
| BstKTI | GATC | 5 cut(s) | 9, 57, 90, 318, 346 |
| BstMBI | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 118 |
| BstSFI | CTRYAG | 2 cut(s) | 66, 294 |
| BstV2I | GAAGAC | 1 cut(s) | 251 |
| BstX2I | RGATCY | 4 cut(s) | 6, 54, 87, 315 |
| BstYI | RGATCY | 4 cut(s) | 6, 54, 87, 315 |
| BtsCI | GGATG | 1 cut(s) | 233 |
| CviJI | RGCY | 2 cut(s) | 127, 285 |
| CviKI_1 | RGCY | 2 cut(s) | 127, 285 |
| DpnI | GATC | 5 cut(s) | 8, 56, 89, 317, 345 |
| DpnII | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| EciI | GGCGGA | 1 cut(s) | 99 |
| FaiI | YATR | 4 cut(s) | 68, 123, 131, 296 |
| FaqI | GGGAC | 1 cut(s) | 118 |
| FauI | CCCGC | 1 cut(s) | 212 |
| FbaI | TGATCA | 1 cut(s) | 343 |
| FokI | GGATG | 1 cut(s) | 240 |
| HapII | CCGG | 1 cut(s) | 138 |
| HinfI | GANTC | 3 cut(s) | 106, 191, 333 |
| HpaII | CCGG | 1 cut(s) | 138 |
| Hpy166II | GTNNAC | 1 cut(s) | 280 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 289 |
| Hpy188III | TCNNGA | 5 cut(s) | 44, 138, 184, 272, 341 |
| Hpy8I | GTNNAC | 1 cut(s) | 280 |
| HpyAV | CCTTC | 2 cut(s) | 181, 323 |
| HpyCH4III | ACNGT | 1 cut(s) | 265 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 170, 202, 231 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 118 |
| Kpn2I | TCCGGA | 1 cut(s) | 137 |
| Ksp22I | TGATCA | 1 cut(s) | 343 |
| Kzo9I | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| LpnPI | CCDG | 5 cut(s) | 126, 131, 151, 197, 240 |
| LweI | GCATC | 2 cut(s) | 211, 218 |
| MaeIII | GTNAC | 1 cut(s) | 265 |
| MalI | GATC | 5 cut(s) | 8, 56, 89, 317, 345 |
| MboI | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| MboII | GAAGA | 3 cut(s) | 164, 256, 325 |
| MflI | RGATCY | 4 cut(s) | 6, 54, 87, 315 |
| MlyI | GAGTC | 1 cut(s) | 100 |
| MmeI | TCCRAC | 1 cut(s) | 129 |
| MnlI | CCTC | 2 cut(s) | 268, 348 |
| MroI | TCCGGA | 1 cut(s) | 137 |
| MseI | TTAA | 3 cut(s) | 15, 78, 306 |
| MspI | CCGG | 1 cut(s) | 138 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 118 |
| NdeII | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| NmuCI | GTSAC | 1 cut(s) | 265 |
| PfeI | GAWTC | 2 cut(s) | 191, 333 |
| PleI | GAGTC | 1 cut(s) | 100 |
| PpsI | GAGTC | 1 cut(s) | 100 |
| PsuI | RGATCY | 4 cut(s) | 6, 54, 87, 315 |
| SaqAI | TTAA | 3 cut(s) | 15, 78, 306 |
| Sau3AI | GATC | 5 cut(s) | 6, 54, 87, 315, 343 |
| SchI | GAGTC | 1 cut(s) | 100 |
| SetI | ASST | 3 cut(s) | 200, 279, 287 |
| SfaNI | GCATC | 2 cut(s) | 211, 218 |
| SfcI | CTRYAG | 2 cut(s) | 66, 294 |
| SmlI | CTYRAG | 1 cut(s) | 272 |
| SmoI | CTYRAG | 1 cut(s) | 272 |
| SsiI | CCGC | 2 cut(s) | 110, 219 |
| TaaI | ACNGT | 1 cut(s) | 265 |
| TaqI | TCGA | 2 cut(s) | 213, 251 |
| TfiI | GAWTC | 2 cut(s) | 191, 333 |
| Tru1I | TTAA | 3 cut(s) | 15, 78, 306 |
| Tru9I | TTAA | 3 cut(s) | 15, 78, 306 |
| TseFI | GTSAC | 1 cut(s) | 265 |
| Tsp45I | GTSAC | 1 cut(s) | 265 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 98 |