AT1G65920

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0019596
Physical Location & Seq
Reverse (-)
24525102 .. 24529362
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65920.2

Sequence Viewer

Length: 3021 bp
ATGGGCGAACAACAAATCTCAGTTACAGTGCCTCGTGATAGAACTGATGAACAGGCAATCTTGGCTTTGAAGAAAGGTGCACAGCTTTTGAAATGTCGAAGAAGAGGAAACCCAAAGTTTTGTCCTTTTAAACTCTCAATGGATGAGAAGTACTTGATTTGGTACTCAGGAGAAGAGGAGAGACAATTGAGATTGAGTTCTGTTATAACGATTGTTCGCGGACAAATCACACCAAACTTTCAGAAACAAGCACAATCTGATCGAAAGGAACAGTCTTTTTCACTCATTTATGCAAATGGAGAACACACTCTTGATCTCATATGTAAGGATAAAGCACAAGCAGACTCATGGTTTAAAGGTCTTAGAGCTGTGATAACAAAGCATCATAACATTAGAAACTCTGTGAATCATCGGAGTAGTAGGGGAGCTCAAAGTTGCATCAATAGTCCTGCGGGTTTTATGCGAAGGAAACAGAATCTTGGACTTCTTGAAGAGACTCCTGACGTCACTCAGATACGTAGCTTATGTGGAAGTCCATCGACGTTACTTGAAGAGCGGTGTTTGTCGAACGGGTTGTCATGTTCTTCAGATAGTTTTGCTGAATCTGATGCCTTGGGTCCAGTTTCTTCTTACTATGAAACAGATTATGATTTCAGGAACTCGGATTGTGATCGTAGTACAGGTTCAGAACTTTGCAGGTTTTCATCACAAAGATTTGCTGCTTCTCCTCCTCTTTCTATCATAACACAGCCGGTTACAAGATCCAATGTCCTTAAGGACATTATGATATGGGGAGCTATAACGGGTCTTATCGATGGATCGAAGAATCAGAACGATGCGTTGTCTCCTAAGCTTTTGGAATCTGCTACGATGTTTGATGTACAGAGTATATCTCTAGGTGCAAAACATGCTGCGTTGGTTACAAGACAAGGTGAAGTGTTTTGTTGGGGGAATGGAAATAGTGGGAAGCTTGGACTTAAAGTTAACATCGATATCGACCATCCGAAACGCGTTGAGTCTCTTGAGGATGTTGCAGTTCGATCAGTGGCTTGTAGTGATCATCAAACGTGTGCTGTTACGGAATCTGGTGAGCTGTATTTATGGGGAATTGATGGTGGAACCATTGAACAGTCAGGAAGCCAGTTCTTGACTCGGAAGATATCCGATGTTCTTGGCGGATCTTTGACTGTTTTAAGTGTTGCTTGTGGCGCGTGGCACACGGCAATTGTGACTAGTTCTGGTCAGCTTTTCACTTATGGTAGTGGAACTTTTGGAGTTCTTGGACATGGGAGTCTTGAGAGTGTTACAAAGCCGAAAGAAGTTGAGTCTTTGAGACGCATGAAGGTCATATCTGTCTCTTGTGGACCGTGGCACACGGCTGCTATAGTGGAAACCGCGAACGACCGGAAATTCTACAACGCCAAGAGTTGTGGAAAGCTGTTTACTTGGGGAGATGGCGATAAAGGACGGTTAGGACATGCTGATAGCAAGAGAAAGCTTGTGCCAACTTGTGTTACTGAACTCATTGATCATGATTTCATAAAAGTCTCTTGTGGATGGACTTTAACTGTTGCTTTAAGCATTTCAGGAACGGTTTATACAATGGGAAGCTCGATCCACGGACAGTTAGGATGTCCGCGGGCAAAGGATAAGTCGGTAAATGTTGTTTTAGGCAATCTAACACGACAATTTGTCAAGGATATTGCTTCAGGTTCACATCATGTAGCTGTTCTAACTTCATTTGGAAATGTTTATACTTGGGGAAAAGGCATGAATGGACAACTTGGTTTAGGTGACGTTAGAGATAGAAACTCTCCTGTTCTTGTTGAGCCTTTAGGTGATAGGTTGGTAGAAAGTATAGCTTGCGGGTTGAATTTGACGGCTGCGATCTGTTTACACAAAGAGATCTCTTTGAATGATCAAACCGCTTGTAGTTCTTGCAAATCAGCTTTTGGATTCACAAGGAGAAAGCATAACTGTTACAACTGTGGTCTATTGTTTTGCAATGCATGTAGTAGCAAGAAAGCGGTAAATGCTTCGTTAGCACCGAATAAAAGCAAGCTTTCTCGGGTTTGCGATTCTTGCTTTGATCATCTATGGAGTATAACAGAGTTTTCAAGAAATGTTAAGATGGATAATCACACTCCGAGGATGCAGATGGTTACACGAAGAGTATCTGAGGATTTGACAGAAAAACAATCAGAGAATGAGATGCAGAATCTTCCACAAGCTAATCGATCTTCGGATGGACAGCCTCGATGGGGACAAGTCTCTGGTCCTTCTCTGTTTCGTTTCGATAAGATTTCGACAAGTTCTTCTTTGAATCTTTCAGTCTCAGCAAGGCGAACATCTTCAACGAAGATTTCAACCTCGTCTGAGTCGAATAAGATCTTAACCGAAGAAATCGAAAGGCTAAAAGCCGTGATAAAAAATCTCCAGAGGCAGTGTGAACTTGGCAATGAGAAGATGGAAGAATGTCAACAAGAGCTCGACAAGACATGGGAAGTTGCTAAAGAAGAAGCAGAGAAATCGAAAGCTGCTAAAGAGATAATAAAAGCCTTGGCATCGAAGCTCCAAGCGAATAAAGAGAAACCGAGTAATCCTCTGAAGACCGGCATTGCATGCAATCCTTCTCAAGTCTCACCAATCTTTGATGACTCAATGTCCATTCCATACCTCACACCAATCACCACAGCTCGTTCACAACACGAAACCAAACAACATGTAGAGAAATGTGTCACGAAATCCTCGAACCGTGACAGCAACATTAAGCTATTAGTAGATGCATCGCCAGCCATCACAAGAACAGGGTATCTCCAAAACGAAACACAAGACTCGTCAGCAGAACAAGTTGAGCAATACGAGCCCGGTGTATATATAACTTTCACGGCCTTGCCTTGCGGTCAGAAGACGCTTAAACGCGTACGGTTTAGTCGGAAGAGGTTTTCAGAGAAGGAAGCACAAAGATGGTGGGAAGAGAAGCAGGTTTTGGTCTATAACAAGTATGATGCTGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1006

Amino Acids

111.11

Weight (kDa)

8.77

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 13 - 77 3.5e-08 Pleckstrin homology domain
WD40_RLD PF25390 216 - 582 2.5e-70 RCC1-like domain
RCC1_2 PF13540 248 - 276 3.4e-07 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 256 - 372 3e-08 Alsin RCC1-like domain
RCC1 PF00415 264 - 313 4.2e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 352 - 379 4.8e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 367 - 416 4.5e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 432 - 480 2.4e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 483 - 532 9.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 536 - 584 3.4e-13 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 593 - 653 1.2e-14 FYVE zinc finger
BRX_N PF13713 793 - 826 7.3e-08 Transcription factor BRX N-terminal domain
BRX PF08381 902 - 956 8.4e-26 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019596)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65920 AT1G65920
malus_domestica MD09G1279800.v1.1
prunus_persica Prupe.3G014600_v2.0.a1 Prupe.3G014600_v2.0.a1 Prupe.3G014600_v2.0.a1
pyrus_communis pycom09g18800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AasI GACNNNNNNGTC 1 cut(s) 1290
AatII GACGTC 1 cut(s) 507
Acc36I ACCTGC 2 cut(s) 687, 2974
AccBSI CCGCTC 1 cut(s) 556
AccII CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
AclWI GGATC 4 cut(s) 756, 826, 1186, 1609
AcsI RAATTY 2 cut(s) 1409, 1873
AcuI CTGAAG 3 cut(s) 570, 1692, 2627
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 5 cut(s) 152, 164, 679, 882, 2925
AfiI CCNNNNNNNGG 1 cut(s) 2261
AflII CTTAAG 1 cut(s) 773
AflIII ACRYGT 4 cut(s) 1009, 1067, 2722, 2920
AhdI GACNNNNNGTC 2 cut(s) 1691, 2662
AhlI ACTAGT 1 cut(s) 1232
AjuI GAANNNNNNNTTGG 2 cut(s) 2972, 3004
Alw21I GWGCWC 3 cut(s) 82, 430, 2490
Alw44I GTGCAC 1 cut(s) 78
AlwI GGATC 4 cut(s) 756, 826, 1186, 1609
Ama87I CYCGRG 1 cut(s) 2067
AoxI GGCC 1 cut(s) 2889
ApaLI GTGCAC 1 cut(s) 78
ApeKI GCWGC 5 cut(s) 719, 911, 1379, 1883, 2537
ApoI RAATTY 2 cut(s) 1409, 1873
ArsI GACNNNNNNTTYG 4 cut(s) 560, 592, 1637, 1669
Asp700I GAANNNNTTC 1 cut(s) 2350
AspLEI GCGC 1 cut(s) 1211
AspS9I GGNCC 3 cut(s) 617, 1364, 2276
AsuC2I CCSGG 1 cut(s) 2868
AsuHPI GGTGA 6 cut(s) 944, 1100, 1805, 1850, 2634, 2680
AvaI CYCGRG 1 cut(s) 2067
AvaII GGWCC 3 cut(s) 617, 1364, 2276
BaeGI GKGCMC 1 cut(s) 82
BanII GRGCYC 3 cut(s) 430, 2490, 2868
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 2615, 2915
Bbv12I GWGCWC 3 cut(s) 82, 430, 2490
BbvI GCAGC 5 cut(s) 706, 898, 1366, 1870, 2524
BceAI ACGGC 5 cut(s) 1236, 1392, 1896, 2405, 2904
BcgI CGANNNNNNTGC 2 cut(s) 2511, 2545
BclI TGATCA 4 cut(s) 1057, 1528, 1918, 2089
BcnI CCSGG 1 cut(s) 2868
BcuI ACTAGT 1 cut(s) 1232
BfaI CTAG 2 cut(s) 896, 1233
BfmI CTRYAG 1 cut(s) 1383
BfrI CTTAAG 1 cut(s) 773
BfuAI ACCTGC 2 cut(s) 687, 2974
BglII AGATCT 2 cut(s) 1905, 2388
BisI GCNGC 5 cut(s) 720, 912, 1380, 1884, 2538
BlsI GCNGC 5 cut(s) 721, 913, 1381, 1885, 2539
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 2868
Bme18I GGWCC 3 cut(s) 617, 1364, 2276
BmeRI GACNNNNNGTC 2 cut(s) 1691, 2662
BmeT110I CYCGRG 1 cut(s) 2067
BmgT120I GGNCC 3 cut(s) 617, 1364, 2276
BmiI GGNNCC 2 cut(s) 618, 1120
BmrFI CCNGG 1 cut(s) 2868
BpiI GAAGAC 2 cut(s) 2615, 2915
BplI GAGNNNNNCTC 4 cut(s) 877, 909, 2587, 2619
BpmI CTGGAG 1 cut(s) 2420
Bpu10I CCTNAGC 1 cut(s) 849
BpuEI CTTGAG 3 cut(s) 1043, 1316, 2619
BpuMI CCSGG 1 cut(s) 2868
Bsa29I ATCGAT 3 cut(s) 813, 990, 2236
BsaAI YACGTR 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 669
BsaHI GRCGYC 1 cut(s) 504
BsaJI CCNNGG 6 cut(s) 612, 1367, 1618, 1637, 2147, 2559
BsaWI WCCGGW 1 cut(s) 1404
Bsc4I CCNNNNNNNGG 1 cut(s) 2261
Bse118I RCCGGY 2 cut(s) 751, 2612
Bse1I ACTGG 2 cut(s) 620, 1141
Bse3DI GCAATG 3 cut(s) 2011, 2464, 2616
Bse8I GATNNNNATC 1 cut(s) 669
BseCI ATCGAT 3 cut(s) 813, 990, 2236
BseDI CCNNGG 6 cut(s) 612, 1367, 1618, 1637, 2147, 2559
BseGI GGATG 7 cut(s) 148, 1000, 1033, 1562, 1637, 2157, 2251
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 1 cut(s) 2261
BseMI GCAATG 3 cut(s) 2011, 2464, 2616
BseMII CTCAG 6 cut(s) 33, 180, 524, 2169, 2349, 2367
BseNI ACTGG 2 cut(s) 620, 1141
BseRI GAGGAG 3 cut(s) 191, 717, 720
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 5 cut(s) 706, 898, 1366, 1870, 2524
Bsh1236I CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
Bsh1285I CGRYCG 1 cut(s) 1405
BshFI GGCC 1 cut(s) 2891
BshVI ATCGAT 3 cut(s) 813, 990, 2236
BsiEI CGRYCG 1 cut(s) 1405
BsiHKAI GWGCWC 3 cut(s) 82, 430, 2490
BsiHKCI CYCGRG 1 cut(s) 2067
BsiSI CCGG 4 cut(s) 752, 1405, 2613, 2868
BsiWI CGTACG 1 cut(s) 2923
BslFI GGGAC 1 cut(s) 2277
BslI CCNNNNNNNGG 1 cut(s) 2261
BsmBI CGTCTC 1 cut(s) 1327
BsmFI GGGAC 1 cut(s) 2277
BsnI GGCC 1 cut(s) 2891
BsoBI CYCGRG 1 cut(s) 2067
Bsp1286I GDGCHC 4 cut(s) 82, 430, 2490, 2868
Bsp1407I TGTACA 1 cut(s) 880
BspANI GGCC 1 cut(s) 2891
BspCNI CTCAG 6 cut(s) 32, 179, 523, 2170, 2348, 2368
BspDI ATCGAT 3 cut(s) 813, 990, 2236
BspFNI CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
BspHI TCATGA 1 cut(s) 1531
BspLI GGNNCC 2 cut(s) 618, 1120
BspMI ACCTGC 2 cut(s) 687, 2974
BspPI GGATC 4 cut(s) 756, 826, 1186, 1609
BspQI GCTCTTC 1 cut(s) 546
BspTI CTTAAG 1 cut(s) 773
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 3 cut(s) 2011, 2464, 2616
BsrFI RCCGGY 2 cut(s) 751, 2612
BsrGI TGTACA 1 cut(s) 880
BsrI ACTGG 2 cut(s) 620, 1141
BssAI RCCGGY 2 cut(s) 751, 2612
BssECI CCNNGG 6 cut(s) 612, 1367, 1618, 1637, 2147, 2559
BssNI GRCGYC 1 cut(s) 504
BssSI CACGAG 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 612, 2559
Bst2BI CACGAG 1 cut(s) 33
Bst6I CTCTTC 7 cut(s) 97, 168, 486, 546, 2164, 2933, 2970
BstACI GRCGYC 1 cut(s) 504
BstAFI CTTAAG 1 cut(s) 773
BstAPI GCANNNNNTGC 2 cut(s) 908, 2622
BstAUI TGTACA 1 cut(s) 880
BstBAI YACGTR 1 cut(s) 518
BstC8I GCNNGC 5 cut(s) 1641, 1864, 2060, 2623, 2793
BstDEI CTNAG 8 cut(s) 19, 166, 362, 510, 849, 2178, 2335, 2376
BstDSI CCRYGG 3 cut(s) 1367, 1618, 1637
BstF5I GGATG 7 cut(s) 148, 1000, 1033, 1562, 1637, 2157, 2251
BstFNI CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
BstHHI GCGC 1 cut(s) 1211
BstMCI CGRYCG 1 cut(s) 1405
BstMWI GCNNNNNNNGC 9 cut(s) 62, 908, 1208, 2033, 2042, 2082, 2622, 2792, 2863
BstNSI RCATGY 5 cut(s) 911, 1481, 2013, 2625, 2726
BstSCI CCNGG 1 cut(s) 2866
BstSFI CTRYAG 1 cut(s) 1383
BstSLI GKGCMC 1 cut(s) 82
BstSNI TACGTA 1 cut(s) 518
BstUI CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
BstV1I GCAGC 5 cut(s) 706, 898, 1366, 1870, 2524
BstV2I GAAGAC 2 cut(s) 2615, 2915
BstX2I RGATCY 4 cut(s) 761, 1178, 1905, 2388
BstYI RGATCY 4 cut(s) 761, 1178, 1905, 2388
Bsu15I ATCGAT 3 cut(s) 813, 990, 2236
BsuRI GGCC 1 cut(s) 2891
BsuTUI ATCGAT 3 cut(s) 813, 990, 2236
BtgI CCRYGG 3 cut(s) 1367, 1618, 1637
BtgZI GCGATG 1 cut(s) 2772
BtsCI GGATG 7 cut(s) 148, 1000, 1033, 1562, 1637, 2157, 2251
BtsI GCAGTG 1 cut(s) 2450
BtsIMutI CAGTG 3 cut(s) 33, 1050, 2450
BveI ACCTGC 2 cut(s) 687, 2974
Cac8I GCNNGC 5 cut(s) 1641, 1864, 2060, 2623, 2793
CciI TCATGA 1 cut(s) 1531
CfoI GCGC 1 cut(s) 1211
Cfr10I RCCGGY 2 cut(s) 751, 2612
Cfr13I GGNCC 3 cut(s) 617, 1364, 2276
Cfr42I CCGCGG 1 cut(s) 1640
ClaI ATCGAT 3 cut(s) 813, 990, 2236
CseI GACGC 2 cut(s) 1344, 2920
Csp6I GTAC 5 cut(s) 151, 163, 678, 881, 2924
CspCI CAANNNNNGTGG 4 cut(s) 1411, 1446, 2951, 2986
CviQI GTAC 5 cut(s) 151, 163, 678, 881, 2924
DdeI CTNAG 8 cut(s) 19, 166, 362, 510, 849, 2178, 2335, 2376
DraI TTTAAA 2 cut(s) 130, 355
DrdI GACNNNNNNGTC 1 cut(s) 1290
DriI GACNNNNNGTC 2 cut(s) 1691, 2662
DseDI GACNNNNNNGTC 1 cut(s) 1290
Eam1104I CTCTTC 7 cut(s) 97, 168, 486, 546, 2164, 2933, 2970
Eam1105I GACNNNNNGTC 2 cut(s) 1691, 2662
EarI CTCTTC 7 cut(s) 97, 168, 486, 546, 2164, 2933, 2970
EciI GGCGGA 1 cut(s) 1191
Ecl136II GAGCTC 2 cut(s) 428, 2488
Eco105I TACGTA 1 cut(s) 518
Eco130I CCWWGG 2 cut(s) 612, 2559
Eco24I GRGCYC 3 cut(s) 430, 2490, 2868
Eco32I GATATC 2 cut(s) 994, 1161
Eco47I GGWCC 3 cut(s) 617, 1364, 2276
Eco53kI GAGCTC 2 cut(s) 428, 2488
Eco57I CTGAAG 3 cut(s) 570, 1692, 2627
Eco88I CYCGRG 1 cut(s) 2067
EcoICRI GAGCTC 2 cut(s) 428, 2488
EcoRV GATATC 2 cut(s) 994, 1161
EcoT14I CCWWGG 2 cut(s) 612, 2559
EcoT22I ATGCAT 2 cut(s) 2011, 2788
EcoT38I GRGCYC 3 cut(s) 430, 2490, 2868
ErhI CCWWGG 2 cut(s) 612, 2559
Esp3I CGTCTC 1 cut(s) 1327
FalI AAGNNNNNCTT 6 cut(s) 1186, 1218, 1846, 1878, 2302, 2334
FaqI GGGAC 1 cut(s) 2277
FauI CCCGC 3 cut(s) 445, 1632, 1859
FauNDI CATATG 1 cut(s) 320
FbaI TGATCA 4 cut(s) 1057, 1528, 1918, 2089
Fnu4HI GCNGC 5 cut(s) 720, 912, 1380, 1884, 2538
FokI GGATG 7 cut(s) 155, 987, 1040, 1569, 1644, 2164, 2258
FriOI GRGCYC 3 cut(s) 430, 2490, 2868
Fsp4HI GCNGC 5 cut(s) 720, 912, 1380, 1884, 2538
FspBI CTAG 2 cut(s) 896, 1233
GlaI GCGC 1 cut(s) 1210
GluI GCNGC 5 cut(s) 720, 912, 1380, 1884, 2538
GsuI CTGGAG 1 cut(s) 2420
HaeIII GGCC 1 cut(s) 2891
HapII CCGG 4 cut(s) 752, 1405, 2613, 2868
HgaI GACGC 2 cut(s) 1344, 2920
HhaI GCGC 1 cut(s) 1211
Hin1I GRCGYC 1 cut(s) 504
Hin6I GCGC 1 cut(s) 1209
HinP1I GCGC 1 cut(s) 1209
HincII GTYRAC 2 cut(s) 985, 2480
HindII GTYRAC 2 cut(s) 985, 2480
HindIII AAGCTT 4 cut(s) 851, 968, 1496, 2060
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 4 cut(s) 752, 1405, 2613, 2868
HphI GGTGA 6 cut(s) 944, 1100, 1805, 1850, 2634, 2680
Hpy166II GTNNAC 9 cut(s) 80, 985, 1364, 1443, 1715, 1895, 2450, 2480, 2702
Hpy8I GTNNAC 9 cut(s) 80, 985, 1364, 1443, 1715, 1895, 2450, 2480, 2702
Hpy99I CGWCG 1 cut(s) 544
HpyAV CCTTC 5 cut(s) 459, 1336, 2289, 2640, 2947
HpyCH4IV ACGT 5 cut(s) 504, 517, 542, 1067, 1797
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 908, 1208, 2033, 2042, 2082, 2622, 2792, 2863
HpyF3I CTNAG 8 cut(s) 19, 166, 362, 510, 849, 2178, 2335, 2376
HpySE526I ACGT 5 cut(s) 504, 517, 542, 1067, 1797
Hsp92I GRCGYC 1 cut(s) 504
HspAI GCGC 1 cut(s) 1209
Ksp22I TGATCA 4 cut(s) 1057, 1528, 1918, 2089
KspAI GTTAAC 1 cut(s) 985
KspI CCGCGG 1 cut(s) 1640
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 3 cut(s) 425, 794, 2577
Lsp1109I GCAGC 5 cut(s) 706, 898, 1366, 1870, 2524
MaeI CTAG 2 cut(s) 896, 1233
MaeII ACGT 5 cut(s) 504, 517, 542, 1067, 1797
MbiI CCGCTC 1 cut(s) 556
MfeI CAATTG 2 cut(s) 185, 1224
MflI RGATCY 4 cut(s) 761, 1178, 1905, 2388
MhlI GDGCHC 4 cut(s) 82, 430, 2490, 2868
MluCI AATT 6 cut(s) 185, 1107, 1224, 1409, 1688, 1873
MluI ACGCGT 2 cut(s) 1009, 2920
MlyI GAGTC 9 cut(s) 338, 490, 1025, 1144, 1300, 1334, 2387, 2651, 2828
MmeI TCCRAC 1 cut(s) 2915
Mph1103I ATGCAT 2 cut(s) 2011, 2788
MroXI GAANNNNTTC 1 cut(s) 2350
MslI CAYNNNNRTG 1 cut(s) 2965
MspA1I CMGCKG 1 cut(s) 1639
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 4 cut(s) 752, 1405, 2613, 2868
MspR9I CCNGG 1 cut(s) 2868
MunI CAATTG 2 cut(s) 185, 1224
MvnI CGCG 6 cut(s) 219, 1011, 1211, 1397, 1639, 2922
MwoI GCNNNNNNNGC 9 cut(s) 62, 908, 1208, 2033, 2042, 2082, 2622, 2792, 2863
NciI CCSGG 1 cut(s) 2868
NdeI CATATG 1 cut(s) 320
NlaIV GGNNCC 2 cut(s) 618, 1120
NmuCI GTSAC 5 cut(s) 505, 1228, 1793, 2737, 2756
NsiI ATGCAT 2 cut(s) 2011, 2788
NspI RCATGY 5 cut(s) 911, 1481, 2013, 2625, 2726
PaeI GCATGC 1 cut(s) 2625
PagI TCATGA 1 cut(s) 1531
PciI ACATGT 1 cut(s) 2722
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 3 cut(s) 2045, 2378, 2747
PdmI GAANNNNTTC 1 cut(s) 2350
Pfl23II CGTACG 1 cut(s) 2923
PkrI GCNGC 5 cut(s) 721, 913, 1381, 1885, 2539
PleI GAGTC 9 cut(s) 338, 490, 1024, 1144, 1299, 1333, 2386, 2651, 2828
PpsI GAGTC 9 cut(s) 338, 490, 1024, 1144, 1299, 1333, 2386, 2651, 2828
Ppu21I YACGTR 1 cut(s) 518
PscI ACATGT 1 cut(s) 2722
PsiI TTATAA 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 430, 2490
PspLI CGTACG 1 cut(s) 2923
PspN4I GGNNCC 2 cut(s) 618, 1120
PspPI GGNCC 3 cut(s) 617, 1364, 2276
PsrI GAACNNNNNNTAC 4 cut(s) 667, 699, 1582, 1614
PsuI RGATCY 4 cut(s) 761, 1178, 1905, 2388
RsaI GTAC 5 cut(s) 152, 164, 679, 882, 2925
RsaNI GTAC 5 cut(s) 151, 163, 678, 881, 2924
RseI CAYNNNNRTG 1 cut(s) 2965
SacI GAGCTC 2 cut(s) 430, 2490
SacII CCGCGG 1 cut(s) 1640
SapI GCTCTTC 1 cut(s) 546
SatI GCNGC 5 cut(s) 720, 912, 1380, 1884, 2538
Sau96I GGNCC 3 cut(s) 617, 1364, 2276
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 9 cut(s) 338, 490, 1025, 1144, 1300, 1334, 2387, 2651, 2828
ScrFI CCNGG 1 cut(s) 2868
SduI GDGCHC 4 cut(s) 82, 430, 2490, 2868
SfcI CTRYAG 1 cut(s) 1383
Sfr303I CCGCGG 1 cut(s) 1640
SgrBI CCGCGG 1 cut(s) 1640
SinI GGWCC 3 cut(s) 617, 1364, 2276
SmiMI CAYNNNNRTG 1 cut(s) 2965
SmlI CTYRAG 4 cut(s) 773, 1022, 1295, 2634
SmoI CTYRAG 4 cut(s) 773, 1022, 1295, 2634
SnaBI TACGTA 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 1232
SphI GCATGC 1 cut(s) 2625
Sse9I AATT 6 cut(s) 185, 1107, 1224, 1409, 1688, 1873
SspMI CTAG 2 cut(s) 896, 1233
SstI GAGCTC 2 cut(s) 430, 2490
StyD4I CCNGG 1 cut(s) 2866
StyI CCWWGG 2 cut(s) 612, 2559
TaiI ACGT 5 cut(s) 507, 520, 545, 1070, 1800
TasI AATT 6 cut(s) 185, 1107, 1224, 1409, 1688, 1873
TatI WGTACW 3 cut(s) 150, 677, 880
TscAI CASTG 3 cut(s) 33, 1050, 2450
TseFI GTSAC 5 cut(s) 505, 1228, 1793, 2737, 2756
TseI GCWGC 5 cut(s) 719, 911, 1379, 1883, 2537
Tsp45I GTSAC 5 cut(s) 505, 1228, 1793, 2737, 2756
TspDTI ATGAA 7 cut(s) 63, 651, 693, 1355, 1528, 1728, 1787
TspGWI ACGGA 2 cut(s) 1094, 1635
TspRI CASTG 3 cut(s) 33, 1050, 2450
Vha464I CTTAAG 1 cut(s) 773
VneI GTGCAC 1 cut(s) 78
VpaK11BI GGWCC 3 cut(s) 617, 1364, 2276
XapI RAATTY 2 cut(s) 1409, 1873
XceI RCATGY 5 cut(s) 911, 1481, 2013, 2625, 2726
XmnI GAANNNNTTC 1 cut(s) 2350
XspI CTAG 2 cut(s) 896, 1233
ZraI GACGTC 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 2 cut(s) 2011, 2788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.