MD09G1279800.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0019596
Physical Location & Seq
Forward (+)
35657777 .. 35664110
6334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1279800.v1.1.491

Sequence Viewer

Length: 3108 bp
ATGGACGAACATTCTTTGTCAACTGTTGCATTTGACAGAGCAATACAACAGGCAGTTGTCTCTATGAAGAGGGGTGCTTATCTGCTGAAATATGGACGAAGAGGGAAGCCCAAGTTCTGCCCTTTCCGGCTTTCCACGGATGAGAAGTTTTTGATATGGTGTTCCAGAAAGCAGGAAAGGCAATTGAAATTGAGCTCAGTCGCAAAAATTATACCCGGACAGTGGACAGTTAGTTTACGGGGGAAACTGGAACCAGAAAGGAAGGGTCAGTCATTTTCAATTGTCTATGCACACAGTGAATGTTCACTTGATCTGATGTGCAAAGATGAAATCCAATGTGATCTTTGGATTTTAGGGTTGAGGGCTTTAACATCCCTGCATCGCCATCCTAGACCACTTAATAATTTGGGGAGTTTCAGACAGGTGCAAAGTTGTCTAAATAGTCCGGCAGGTCTTTTTAGGAGAAGGCAAACTCTTGGAATAGTGGATGATGACAACGAGTTCTCCCAGGTGAAAAGCGTATGCGGGAGTCCTACTTTAACACTTTCTGAGAGGTGTATTTCTGATGGCATATCTTTTTCTTCTGATAGCTTTTATTCGTCGGAGTCAGTCCTATCAAATATGCAAAATGTAATGGACATCTCAGTTCCAGATTCTCCATATTTTGGATCAGATTATCTCAAGAGGAGGCGAATATATTCTGATGCCGACTGTGAGCCTCACAGGTTTGTTTCCTATGCCTATGGAACCTCAAGAATTGAAAAGGACAATATTTTGAAAGATGTCATGATCTGGGGTGAAAGTATTGGAGGGAATGTAAGTGGATTGCAAGCAGATGCTTTACTGCCTAAGTTATTAGAATCAACTATGACAATGGATGTACAGAATATATCTTTAGGAGGGAAGCATGCTGCAGTAGTTACGAAGCAGGGGGAAGTGTTCTGCTGGGGTCACGCTAATGGGGGAAGACTAGGGCACAAAATCAATATGGATGTGAGCAACCCTAAACCTGTTGACTCCCTTAATGGGATCCAGGTGAAATCTGTTTCGTGTGGTGAATATCAAACATGCGCTCTGACACATTCTGGTGAGCTCTATACATGGGGAGACACAAGTTGCGGTGCCAGTTTAGAGGCCGAGGAGAAGAGTATCAGTCAGTGGTTACCGCATAAACTTTCTGGTCCGTTGAATAATATAAATGTATCATATGTCGCTTGTGGGGAATGGCATACAGCTATGGTGTCCACTTCTGGGAATTTATTTACATATGGAGACGGAACCTTTGGGGTACTGGGGCACGGAAATCTTTATAGTGTTTATCAACCAAAGCAAGTTGAATCTCTCAAGGGTTTATGGGTAAAATCTGCTGCATGCGGATCATGGCACATGGCTGCTGTAGTTGAAATCATGGTCGATTGTCTCAGGTCCAATTCAATTGGTGGGAAGTTGTTTACATGGGGTGATGCGGATAGAGGGAGGCTAGGACATGTGGATCATGAGAAGAAGCTTTTACCAACTTGCGTTGGGCAGCTTGTGGATCATGATTTTGTTCAAGTTTGTTGTGGAAGAATGTTGACTGTTGGGCTTACCAATAAAGGTGCAGTTTATACAATTGGAAGTTCAGTACATGGGCAGTTAGGGAATGCTCAAGCCAAGGATAAATCAATCACATTGGTTGAAGGGAAGCTTAAGGAAGAGTTTGTTAGAGAAATAGCATCCGTTTCATATCATATAGCTGTTTTGACAGCCAGAGGAAATGTTTATACCTGGGGAAAGGGGACTAATGGACAGTTGGGATTAGGTGATATCAACGATCGTAGCACACCAACTTTTGTGGAGGCTTTGAGAGACAAGCAGGTAGAAAGTGTTGTTTGTGGATCAAATTCAACAGCTGCAATCTGCGTTCACAAATCTATTTCTGTCAGCAACCAGTCAGCTTGTTATCGATGCAACCTACCTTTTGGGTTTACTAGGAAGAAGCACAACTGCTACAATTGCGGTCTTCTATTCTGCCATTCGTGTAGCAGTAAGAAGGCTATGGATTCTTCGCTGGCACCCAAGAAAGGCAAGGCTTTTCGAGTCTGTGATTCATGCTTTAGCAATCTGCAGAAACTTACACATTCATGTAGATCATTCAAGCAAGAAAATCATAGTAGCATAAAGCTATTGACCGAAGAAAAGTCAGTCCCAGACAGGAAAGAAGAAAAGGCTGGAGAAACACCTAAGTACGGTCATCTATTATCTATCAAACAGGCTTGTAACAAGAAACGCCAGTCTGGTGTGAGGACCACCACCATGAAAAACCAAGAAGGAAACCAGCAGCATCTAGAACCAGCTTCTTCTTCCTTGAGCGAAGAGCCACGATGGGGCCAAGTCTCATGCCCTGATCTATTTAAACCGTATTATAGAGAAAATTATACAGCACTTAACTCTCTGTCAAAAGATCATTTATCTTCTGTCATCCTAGTGCATTCAGAATCCACAATGTCCGGTGCCAGTAACGCAGAGAAATGCATATCCACGTCAGATGAGATGGTTATTGAAGAAATACAGCGGCTGACAGTTGAGGCACACAACCTTGAAAGGAAATGCAAAATTGGAAGCCAGAAGATTCATGAATGTGAGCAGAAGACTGAGGAAACATGGTCTCTGGCAAGGGATGAAGCGGCTACATGTAAGGCAGCAAAGGAGGTTATAAAAGCTTTGGCGTTAAGGCTTAAAACTATTTCAGAAAAGGCGTCTGCTGGAAAGCAACCAAACAATGGTATAGATACAAAACTTCCTCAACTCACGCCACTGAATACAAATACAAAACTTCCTGAAGTAGATAGTGTATCGGATTCGCCTGCTGTGTTGTCTGATACGCCGAAGTCAGAACCCCATGGTAGAGAAACTAAATCGGCAAAGCTTGAATGGGTAGAACAATTTGAACCTGGAGTGCATCTCACGCTTGTTGTCTTGCCAAATGGACAAAAGGGGTTAAAGCGAGTGAATTTTCGCCGGAAGAAGTTCACTGACAAAGATGTAGAAAGATGGTTGGAGGTAAATCAAGGCTTGATATACCAGAATTATGGCATTGAGGGATATGAGAACTCTACAGAAGATTAA

Protein Analysis

1036

Amino Acids

114.53

Weight (kDa)

8.51

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 124 3.9e-08 Pleckstrin homology domain
WD40_RLD PF25390 261 - 631 9.7e-73 RCC1-like domain
RCC1 PF00415 310 - 359 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 346 - 374 2.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 417 - 466 3.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 481 - 528 5.6e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 568 - 597 1.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 585 - 633 2e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 642 - 704 1.2e-13 FYVE zinc finger
BRX_N PF13713 887 - 914 5.5e-06 Transcription factor BRX N-terminal domain
BRX PF08381 971 - 1024 2.5e-17 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019596)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65920 AT1G65920
malus_domestica MD09G1279800.v1.1
prunus_persica Prupe.3G014600_v2.0.a1 Prupe.3G014600_v2.0.a1 Prupe.3G014600_v2.0.a1
pyrus_communis pycom09g18800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2696
Acc36I ACCTGC 2 cut(s) 440, 1846
AccB1I GGYRCC 3 cut(s) 1121, 2053, 2492
AccB7I CCANNNNNTGG 2 cut(s) 665, 2762
AciI CCGC 8 cut(s) 525, 1119, 1166, 1374, 1466, 1998, 2554, 2666
AclWI GGATC 7 cut(s) 676, 1024, 1037, 1384, 1500, 1545, 1885
AcsI RAATTY 3 cut(s) 1255, 1882, 2992
AcuI CTGAAG 1 cut(s) 2841
AcyI GRCGYC 1 cut(s) 2738
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 4 cut(s) 882, 1290, 1626, 2228
AfiI CCNNNNNNNGG 8 cut(s) 222, 665, 1026, 1251, 2063, 2228, 2366, 2762
AflII CTTAAG 1 cut(s) 1688
AflIII ACRYGT 2 cut(s) 1486, 2672
AjiI CACGTC 1 cut(s) 2523
AjnI CCWGG 4 cut(s) 507, 1032, 1766, 2932
Alw21I GWGCWC 2 cut(s) 197, 1095
Alw26I GTCTC 7 cut(s) 64, 1101, 1266, 1424, 1842, 2380, 2652
AlwI GGATC 7 cut(s) 676, 1024, 1037, 1384, 1500, 1545, 1885
AlwNI CAGNNNCTG 1 cut(s) 2557
AoxI GGCC 2 cut(s) 1134, 2368
ApeKI GCWGC 7 cut(s) 911, 1367, 1391, 1528, 1892, 2320, 2681
ApoI RAATTY 3 cut(s) 1255, 1882, 2992
ArsI GACNNNNNNTTYG 2 cut(s) 1265, 1297
Asp700I GAANNNNTTC 2 cut(s) 697, 3008
AspLEI GCGC 1 cut(s) 1073
AspS9I GGNCC 4 cut(s) 1181, 1425, 2286, 2368
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 7 cut(s) 523, 809, 1048, 1067, 1100, 1472, 1814
AvaII GGWCC 3 cut(s) 1181, 1425, 2286
BaeGI GKGCMC 2 cut(s) 978, 1299
BamHI GGATCC 1 cut(s) 1029
BanI GGYRCC 3 cut(s) 1121, 2053, 2492
BanII GRGCYC 2 cut(s) 197, 1095
BbsI GAAGAC 3 cut(s) 973, 1994, 2636
Bbv12I GWGCWC 2 cut(s) 197, 1095
BbvI GCAGC 7 cut(s) 898, 1354, 1378, 1540, 1879, 2332, 2693
BccI CCATC 5 cut(s) 393, 560, 2358, 2527, 3027
BciT130I CCWGG 4 cut(s) 509, 1034, 1768, 2934
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 7 cut(s) 64, 1101, 1266, 1424, 1842, 2380, 2652
BfaI CTAG 6 cut(s) 390, 971, 1481, 1971, 2327, 2465
BfmI CTRYAG 4 cut(s) 912, 1395, 2105, 3096
BfrI CTTAAG 1 cut(s) 1688
BfuAI ACCTGC 2 cut(s) 440, 1846
BisI GCNGC 9 cut(s) 912, 1368, 1392, 1529, 1893, 2321, 2555, 2667, 2682
BlsI GCNGC 9 cut(s) 913, 1369, 1393, 1530, 1894, 2322, 2556, 2668, 2683
Bme1390I CCNGG 5 cut(s) 216, 509, 1034, 1768, 2934
Bme18I GGWCC 3 cut(s) 1181, 1425, 2286
BmgBI CACGTC 1 cut(s) 2523
BmgT120I GGNCC 4 cut(s) 1181, 1425, 2286, 2368
BmiI GGNNCC 8 cut(s) 252, 748, 1031, 1123, 1279, 2055, 2369, 2494
BmrFI CCNGG 5 cut(s) 216, 509, 1034, 1768, 2934
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 8 cut(s) 388, 694, 826, 1453, 1724, 1937, 2332, 2950
BmuI ACTGGG 1 cut(s) 1301
BpiI GAAGAC 3 cut(s) 973, 1994, 2636
BplI GAGNNNNNCTC 2 cut(s) 2928, 2960
BpmI CTGGAG 2 cut(s) 2232, 2955
BpuEI CTTGAG 5 cut(s) 665, 736, 1328, 1632, 2368
BpuMI CCSGG 1 cut(s) 216
Bsa29I ATCGAT 1 cut(s) 1945
BsaHI GRCGYC 1 cut(s) 2738
BsaI GGTCTC 1 cut(s) 2652
BsaJI CCNNGG 6 cut(s) 135, 507, 1137, 1653, 1767, 2881
BsaWI WCCGGW 1 cut(s) 2489
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 801, 831
Bsc4I CCNNNNNNNGG 8 cut(s) 222, 665, 1026, 1251, 2063, 2228, 2366, 2762
Bse1I ACTGG 6 cut(s) 252, 1125, 1296, 1930, 2272, 2496
BseBI CCWGG 4 cut(s) 509, 1034, 1768, 2934
BseCI ATCGAT 1 cut(s) 1945
BseDI CCNNGG 6 cut(s) 135, 507, 1137, 1653, 1767, 2881
BseGI GGATG 9 cut(s) 145, 371, 385, 493, 883, 997, 1715, 2460, 2665
BseLI CCNNNNNNNGG 8 cut(s) 222, 665, 1026, 1251, 2063, 2228, 2366, 2762
BseMII CTCAG 5 cut(s) 210, 540, 657, 1435, 2625
BseNI ACTGG 6 cut(s) 252, 1125, 1296, 1930, 2272, 2496
BseRI GAGGAG 2 cut(s) 700, 1154
BseSI GKGCMC 2 cut(s) 978, 1299
BseXI GCAGC 7 cut(s) 898, 1354, 1378, 1540, 1879, 2332, 2693
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 1 cut(s) 1620
Bsh1285I CGRYCG 1 cut(s) 1816
BshFI GGCC 2 cut(s) 1136, 2370
BshNI GGYRCC 3 cut(s) 1121, 2053, 2492
BshVI ATCGAT 1 cut(s) 1945
BsiEI CGRYCG 1 cut(s) 1816
BsiHKAI GWGCWC 2 cut(s) 197, 1095
BsiSI CCGG 5 cut(s) 127, 216, 446, 2490, 3001
BslFI GGGAC 2 cut(s) 1792, 2171
BslI CCNNNNNNNGG 8 cut(s) 222, 665, 1026, 1251, 2063, 2228, 2366, 2762
BsmAI GTCTC 7 cut(s) 64, 1101, 1266, 1424, 1842, 2380, 2652
BsmBI CGTCTC 1 cut(s) 1266
BsmFI GGGAC 2 cut(s) 1792, 2171
BsmI GAATGC 2 cut(s) 1648, 2470
BsnI GGCC 2 cut(s) 1136, 2370
Bso31I GGTCTC 1 cut(s) 2652
Bsp1286I GDGCHC 4 cut(s) 197, 978, 1095, 1299
Bsp1407I TGTACA 1 cut(s) 880
Bsp19I CCATGG 1 cut(s) 2881
BspACI CCGC 8 cut(s) 525, 1119, 1166, 1374, 1466, 1998, 2554, 2666
BspANI GGCC 2 cut(s) 1136, 2370
BspCNI CTCAG 5 cut(s) 209, 541, 656, 1434, 2626
BspDI ATCGAT 1 cut(s) 1945
BspHI TCATGA 4 cut(s) 786, 1495, 1540, 2614
BspLI GGNNCC 8 cut(s) 252, 748, 1031, 1123, 1279, 2055, 2369, 2494
BspMAI CTGCAG 2 cut(s) 916, 2109
BspMI ACCTGC 2 cut(s) 440, 1846
BspPI GGATC 7 cut(s) 676, 1024, 1037, 1384, 1500, 1545, 1885
BspQI GCTCTTC 1 cut(s) 2349
BspT107I GGYRCC 3 cut(s) 1121, 2053, 2492
BspTI CTTAAG 1 cut(s) 1688
BspTNI GGTCTC 1 cut(s) 2652
BsrGI TGTACA 1 cut(s) 880
BsrI ACTGG 6 cut(s) 252, 1125, 1296, 1930, 2272, 2496
BssECI CCNNGG 6 cut(s) 135, 507, 1137, 1653, 1767, 2881
BssNI GRCGYC 1 cut(s) 2738
BssT1I CCWWGG 2 cut(s) 1653, 2881
Bst2UI CCWGG 4 cut(s) 509, 1034, 1768, 2934
Bst6I CTCTTC 5 cut(s) 62, 94, 1139, 1689, 2349
BstACI GRCGYC 1 cut(s) 2738
BstAFI CTTAAG 1 cut(s) 1688
BstAUI TGTACA 1 cut(s) 880
BstC8I GCNNGC 5 cut(s) 831, 909, 1372, 2052, 2847
BstDEI CTNAG 7 cut(s) 196, 549, 643, 849, 1421, 2223, 2634
BstDSI CCRYGG 2 cut(s) 135, 2881
BstEII GGTNACC 1 cut(s) 1161
BstF5I GGATG 9 cut(s) 145, 371, 385, 493, 883, 997, 1715, 2460, 2665
BstHHI GCGC 1 cut(s) 1073
BstMAI GTCTC 7 cut(s) 64, 1101, 1266, 1424, 1842, 2380, 2652
BstMCI CGRYCG 1 cut(s) 1816
BstMWI GCNNNNNNNGC 4 cut(s) 178, 1995, 2501, 2947
BstNI CCWGG 4 cut(s) 509, 1034, 1768, 2934
BstNSI RCATGY 5 cut(s) 911, 1071, 1374, 1490, 2676
BstPI GGTNACC 1 cut(s) 1161
BstSCI CCNGG 5 cut(s) 214, 507, 1032, 1766, 2932
BstSFI CTRYAG 4 cut(s) 912, 1395, 2105, 3096
BstSLI GKGCMC 2 cut(s) 978, 1299
BstV1I GCAGC 7 cut(s) 898, 1354, 1378, 1540, 1879, 2332, 2693
BstV2I GAAGAC 3 cut(s) 973, 1994, 2636
BstX2I RGATCY 1 cut(s) 1029
BstXI CCANNNNNNTGG 1 cut(s) 3071
BstYI RGATCY 1 cut(s) 1029
Bsu15I ATCGAT 1 cut(s) 1945
BsuRI GGCC 2 cut(s) 1136, 2370
BsuTUI ATCGAT 1 cut(s) 1945
BtgI CCRYGG 2 cut(s) 135, 2881
BtgZI GCGATG 1 cut(s) 365
BtrI CACGTC 1 cut(s) 2523
BtsCI GGATG 9 cut(s) 145, 371, 385, 493, 883, 997, 1715, 2460, 2665
BtsIMutI CAGTG 5 cut(s) 227, 301, 1163, 2795, 3012
BveI ACCTGC 2 cut(s) 440, 1846
Cac8I GCNNGC 5 cut(s) 831, 909, 1372, 2052, 2847
CaiI CAGNNNCTG 1 cut(s) 2557
CciI TCATGA 4 cut(s) 786, 1495, 1540, 2614
CfoI GCGC 1 cut(s) 1073
Cfr13I GGNCC 4 cut(s) 1181, 1425, 2286, 2368
ClaI ATCGAT 1 cut(s) 1945
CseI GACGC 1 cut(s) 2727
Csp6I GTAC 4 cut(s) 881, 1289, 1625, 2227
CspCI CAANNNNNGTGG 2 cut(s) 1815, 1850
CviQI GTAC 4 cut(s) 881, 1289, 1625, 2227
DdeI CTNAG 7 cut(s) 196, 549, 643, 849, 1421, 2223, 2634
DraI TTTAAA 1 cut(s) 2395
DraIII CACNNNGTG 1 cut(s) 296
Eam1104I CTCTTC 5 cut(s) 62, 94, 1139, 1689, 2349
EarI CTCTTC 5 cut(s) 62, 94, 1139, 1689, 2349
Ecl136II GAGCTC 2 cut(s) 195, 1093
Eco130I CCWWGG 2 cut(s) 1653, 2881
Eco24I GRGCYC 2 cut(s) 197, 1095
Eco31I GGTCTC 1 cut(s) 2652
Eco32I GATATC 1 cut(s) 1807
Eco47I GGWCC 3 cut(s) 1181, 1425, 2286
Eco53kI GAGCTC 2 cut(s) 195, 1093
Eco57I CTGAAG 1 cut(s) 2841
Eco91I GGTNACC 1 cut(s) 1161
EcoICRI GAGCTC 2 cut(s) 195, 1093
EcoO65I GGTNACC 1 cut(s) 1161
EcoRII CCWGG 4 cut(s) 507, 1032, 1766, 2932
EcoRV GATATC 1 cut(s) 1807
EcoT14I CCWWGG 2 cut(s) 1653, 2881
EcoT22I ATGCAT 1 cut(s) 2516
EcoT38I GRGCYC 2 cut(s) 197, 1095
ErhI CCWWGG 2 cut(s) 1653, 2881
Esp3I CGTCTC 1 cut(s) 1266
FalI AAGNNNNNCTT 2 cut(s) 1671, 1703
FaqI GGGAC 2 cut(s) 1792, 2171
FauI CCCGC 1 cut(s) 518
FauNDI CATATG 2 cut(s) 1207, 1267
Fnu4HI GCNGC 9 cut(s) 912, 1368, 1392, 1529, 1893, 2321, 2555, 2667, 2682
FokI GGATG 9 cut(s) 152, 358, 372, 500, 890, 1004, 1702, 2447, 2672
FriOI GRGCYC 2 cut(s) 197, 1095
Fsp4HI GCNGC 9 cut(s) 912, 1368, 1392, 1529, 1893, 2321, 2555, 2667, 2682
FspBI CTAG 6 cut(s) 390, 971, 1481, 1971, 2327, 2465
GlaI GCGC 1 cut(s) 1072
GluI GCNGC 9 cut(s) 912, 1368, 1392, 1529, 1893, 2321, 2555, 2667, 2682
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 2 cut(s) 2232, 2955
HaeIII GGCC 2 cut(s) 1136, 2370
HapII CCGG 5 cut(s) 127, 216, 446, 2490, 3001
HgaI GACGC 1 cut(s) 2727
HhaI GCGC 1 cut(s) 1073
Hin1I GRCGYC 1 cut(s) 2738
Hin6I GCGC 1 cut(s) 1071
HinP1I GCGC 1 cut(s) 1071
HincII GTYRAC 3 cut(s) 21, 1015, 1575
HindII GTYRAC 3 cut(s) 21, 1015, 1575
HindIII AAGCTT 4 cut(s) 1505, 1685, 2700, 2906
HpaII CCGG 5 cut(s) 127, 216, 446, 2490, 3001
HphI GGTGA 7 cut(s) 523, 809, 1048, 1067, 1100, 1472, 1814
Hpy99I CGWCG 1 cut(s) 604
HpyAV CCTTC 5 cut(s) 256, 459, 1673, 2026, 2303
HpyCH4IV ACGT 1 cut(s) 2522
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 1995, 2501, 2947
HpyF3I CTNAG 7 cut(s) 196, 549, 643, 849, 1421, 2223, 2634
HpySE526I ACGT 1 cut(s) 2522
Hsp92I GRCGYC 1 cut(s) 2738
HspAI GCGC 1 cut(s) 1071
LguI GCTCTTC 1 cut(s) 2349
Lsp1109I GCAGC 7 cut(s) 898, 1354, 1378, 1540, 1879, 2332, 2693
LweI GCATC 8 cut(s) 388, 694, 826, 1453, 1724, 1937, 2332, 2950
MaeI CTAG 6 cut(s) 390, 971, 1481, 1971, 2327, 2465
MaeII ACGT 1 cut(s) 2522
MaeIII GTNAC 5 cut(s) 919, 950, 1161, 2258, 2498
MfeI CAATTG 5 cut(s) 182, 279, 1434, 1611, 1993
MflI RGATCY 1 cut(s) 1029
MhlI GDGCHC 4 cut(s) 197, 978, 1095, 1299
MlyI GAGTC 4 cut(s) 538, 614, 1010, 2088
MmeI TCCRAC 2 cut(s) 582, 3018
Mph1103I ATGCAT 1 cut(s) 2516
MroXI GAANNNNTTC 2 cut(s) 697, 3008
MslI CAYNNNNRTG 6 cut(s) 957, 1086, 2122, 2294, 2465, 2619
MspA1I CMGCKG 2 cut(s) 1892, 2554
MspCI CTTAAG 1 cut(s) 1688
MspI CCGG 5 cut(s) 127, 216, 446, 2490, 3001
MspR9I CCNGG 5 cut(s) 216, 509, 1034, 1768, 2934
MunI CAATTG 5 cut(s) 182, 279, 1434, 1611, 1993
Mva1269I GAATGC 2 cut(s) 1648, 2470
MvaI CCWGG 4 cut(s) 509, 1034, 1768, 2934
MwoI GCNNNNNNNGC 4 cut(s) 178, 1995, 2501, 2947
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 2881
NdeI CATATG 2 cut(s) 1207, 1267
NlaIV GGNNCC 8 cut(s) 252, 748, 1031, 1123, 1279, 2055, 2369, 2494
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 1 cut(s) 950
NsiI ATGCAT 1 cut(s) 2516
NspI RCATGY 5 cut(s) 911, 1071, 1374, 1490, 2676
PaeI GCATGC 2 cut(s) 911, 1374
PagI TCATGA 4 cut(s) 786, 1495, 1540, 2614
PciI ACATGT 2 cut(s) 1486, 2672
PciSI GCTCTTC 1 cut(s) 2349
PctI GAATGC 2 cut(s) 1648, 2470
PdmI GAANNNNTTC 2 cut(s) 697, 3008
PfeI GAWTC 8 cut(s) 653, 860, 1337, 2042, 2087, 2477, 2611, 2840
PflMI CCANNNNNTGG 2 cut(s) 665, 2762
PkrI GCNGC 9 cut(s) 913, 1369, 1393, 1530, 1894, 2322, 2556, 2668, 2683
Ple19I CGATCG 1 cut(s) 1816
PleI GAGTC 4 cut(s) 537, 613, 1010, 2087
PpsI GAGTC 4 cut(s) 537, 613, 1010, 2087
PscI ACATGT 2 cut(s) 1486, 2672
PsiI TTATAA 1 cut(s) 2696
Psp124BI GAGCTC 2 cut(s) 197, 1095
Psp6I CCWGG 4 cut(s) 507, 1032, 1766, 2932
PspEI GGTNACC 1 cut(s) 1161
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 4 cut(s) 507, 1032, 1766, 2932
PspN4I GGNNCC 8 cut(s) 252, 748, 1031, 1123, 1279, 2055, 2369, 2494
PspPI GGNCC 4 cut(s) 1181, 1425, 2286, 2368
PstI CTGCAG 2 cut(s) 916, 2109
PstNI CAGNNNCTG 1 cut(s) 2557
PsuI RGATCY 1 cut(s) 1029
PvuI CGATCG 1 cut(s) 1816
PvuII CAGCTG 1 cut(s) 1892
RsaI GTAC 4 cut(s) 882, 1290, 1626, 2228
RsaNI GTAC 4 cut(s) 881, 1289, 1625, 2227
RseI CAYNNNNRTG 6 cut(s) 957, 1086, 2122, 2294, 2465, 2619
SacI GAGCTC 2 cut(s) 197, 1095
SapI GCTCTTC 1 cut(s) 2349
SatI GCNGC 9 cut(s) 912, 1368, 1392, 1529, 1893, 2321, 2555, 2667, 2682
Sau96I GGNCC 4 cut(s) 1181, 1425, 2286, 2368
SchI GAGTC 4 cut(s) 538, 614, 1010, 2088
ScrFI CCNGG 5 cut(s) 216, 509, 1034, 1768, 2934
SduI GDGCHC 4 cut(s) 197, 978, 1095, 1299
SfaNI GCATC 8 cut(s) 388, 694, 826, 1453, 1724, 1937, 2332, 2950
SfcI CTRYAG 4 cut(s) 912, 1395, 2105, 3096
SinI GGWCC 3 cut(s) 1181, 1425, 2286
SmiMI CAYNNNNRTG 6 cut(s) 957, 1086, 2122, 2294, 2465, 2619
SmlI CTYRAG 6 cut(s) 680, 751, 1343, 1647, 1688, 2347
SmoI CTYRAG 6 cut(s) 680, 751, 1343, 1647, 1688, 2347
SphI GCATGC 2 cut(s) 911, 1374
SsiI CCGC 8 cut(s) 525, 1119, 1166, 1374, 1466, 1998, 2554, 2666
SspI AATATT 1 cut(s) 772
SspMI CTAG 6 cut(s) 390, 971, 1481, 1971, 2327, 2465
SstI GAGCTC 2 cut(s) 197, 1095
StyD4I CCNGG 5 cut(s) 214, 507, 1032, 1766, 2932
StyI CCWWGG 2 cut(s) 1653, 2881
TaiI ACGT 1 cut(s) 2525
TaqI TCGA 3 cut(s) 1413, 1945, 2077
TaqII GACCGA 1 cut(s) 2186
TatI WGTACW 2 cut(s) 880, 1624
TauI GCSGC 2 cut(s) 2557, 2669
TfiI GAWTC 8 cut(s) 653, 860, 1337, 2042, 2087, 2477, 2611, 2840
TscAI CASTG 5 cut(s) 227, 301, 1163, 2802, 3019
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 7 cut(s) 911, 1367, 1391, 1528, 1892, 2320, 2681
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 9 cut(s) 80, 342, 1713, 2079, 2112, 2312, 2603, 2631, 2676
TspGWI ACGGA 5 cut(s) 152, 1173, 1290, 1314, 1708
TspRI CASTG 5 cut(s) 227, 301, 1163, 2802, 3019
Van91I CCANNNNNTGG 2 cut(s) 665, 2762
Vha464I CTTAAG 1 cut(s) 1688
VpaK11BI GGWCC 3 cut(s) 1181, 1425, 2286
XapI RAATTY 3 cut(s) 1255, 1882, 2992
XbaI TCTAGA 1 cut(s) 2326
XceI RCATGY 5 cut(s) 911, 1071, 1374, 1490, 2676
XmnI GAANNNNTTC 2 cut(s) 697, 3008
XspI CTAG 6 cut(s) 390, 971, 1481, 1971, 2327, 2465
Zsp2I ATGCAT 1 cut(s) 2516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.