AT2G05320

N-acetylglucosaminyltransferase II (MGAT2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013099
Physical Location & Seq
Forward (+)
1935540 .. 1937464
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G05320.1

Sequence Viewer

Length: 1293 bp
ATGGCAAATCTTTGGAAGAAGCAGAGATTGAGAGATACAGGTTTATGTCGTTTAGGGATTCTATTTGCTGTTACTTTGTCTATAGTTCTGATGTTGGTGTCTGTACCTAGAACTGCTTTGAATGGTTCTTCTATTGACGATGATTTGGATGGTTTAGATAAAGATTTGGAAGCTAAGCTTAATGCTTCATTGCTAAGTGTAGCTAGAGGTAATAGAATGTCTCTAAGGTTGCATAGAAGGAACCATTTTTCGCCTAGAAATACGGATCTGTTCCCGGATTTGGCAAAAGATCGTGTGGTTATCGTCTTGTATGTGCATAATCGGGCTCAGTATTTTCGAGTCACAGTGGAAAGTTTGTCGAAGGTTAAAGGTATAAGTGAGACATTGTTGATTGTTAGTCATGATGGTTACTTTGAAGAGATGAATAGGATTGTGGAGAGTATTAAGTTTTGTCAAGTGAAACAGATTTTCTCGCCTTATTCGCCTCATATATATCGTACTAGCTTCCCGGGTGTGACCCTGAATGATTGTAAGAACAAGGGTGATGAGGCAAAGGGGCATTGTGAAGGTAATCCTGATCAGTATGGGAATCATCGGTCTCCGAAGATTGTATCTTTGAAGCATCACTGGTGGTGGATGATGAACACTGTATGGGATGGGTTGGAAGAGACTAAAGGACATGAGGGGCATATCCTTTTCATTGAAGAAGATCATTTTCTGTTTCCTAATGCCTATCGTAACATACAGACTCTTACGAGGCTGAAACCCGCAAAGTGTCCTGACTGTTTTGCTGCTAATTTAGCACCGTCTGATGTGAAGTCAAGAGGAGAAGGGCTTGAAAGTTTGGTTGCAGAGAGAATGGGAAATGTTGGGTATTCTTTTAATAGAAGTGTGTGGGAGAATATTCATCAGAAGGCAAGAGAGTTTTGTTTCTTTGATGATTACAACTGGGATATAACGATGTGGGCAACGGTTTTCCCGTCGTTTGGTTCCCCGGTGTACACATTGCGAGGGCCTAGGACTAGTGCGGTACACTTTGGAAAATGTGGGTTGCATCAAGGTAGAGGAGATGAGGGTGATTGCATCGATAATGGGGTCGTAAACATAGAAGTTAAGGAAACAGATAAAGTTGTGAACATAAAAGAAGGATGGGGAGTTCGGGTGTATAAGCATCAAGCGGGTTATAAAGCCGGTTTCGAAGGTTGGGGAGGTTGGGGCGATGATAGGGACCGACATTTATGTTTGGATTTTGCCACTATGTATCGTTACAGCAGTAGCAGTGCATCTCCATGA

Protein Analysis

430

Amino Acids

48.95

Weight (kDa)

7.99

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGAT2 PF05060 84 - 414 4.3e-102 N-acetylglucosaminyltransferase II (MGAT2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05320
fragaria_vesca FvH4_6g40640
malus_domestica MD09G1126500.v1.1 MD17G1116800.v1.1
prunus_persica Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0156011
rosa_laevigata RLG00000020881
rosa_multiflora Rmu_sc0000260.1_g000023
rosa_roxburghii Rroxscaffold_2G00093290
rosa_rugosa Rorug02G0451200
rosa_samantha Rh2AG518000 Rh2BG529200 Rh2CG502700 Rh2DG538300
rosa_wichuraiana Rw2G042650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AciI CCGC 3 cut(s) 768, 1028, 1178
AclWI GGATC 1 cut(s) 273
AfaI GTAC 4 cut(s) 105, 499, 1001, 1032
AfiI CCNNNNNNNGG 2 cut(s) 280, 986
AgsI TTSAA 5 cut(s) 121, 416, 619, 704, 839
AhlI ACTAGT 1 cut(s) 1022
AloI GAACNNNNNNTCC 2 cut(s) 1140, 1172
AluBI AGCT 4 cut(s) 173, 178, 203, 504
AluI AGCT 4 cut(s) 173, 178, 203, 504
Alw26I GTCTC 4 cut(s) 225, 374, 603, 662
AlwI GGATC 1 cut(s) 273
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 1 cut(s) 1013
ApeKI GCWGC 1 cut(s) 791
AspA2I CCTAGG 1 cut(s) 1016
AspS9I GGNCC 2 cut(s) 1013, 1228
AsuC2I CCSGG 4 cut(s) 275, 509, 510, 995
AsuHPI GGTGA 2 cut(s) 554, 1088
AsuII TTCGAA 1 cut(s) 1197
AvaI CYCGRG 1 cut(s) 508
AvaII GGWCC 1 cut(s) 1228
AvrII CCTAGG 1 cut(s) 1016
BanII GRGCYC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 778
BccI CCATC 4 cut(s) 143, 398, 650, 1143
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 4 cut(s) 275, 509, 510, 995
BcoDI GTCTC 4 cut(s) 225, 374, 603, 662
BcuI ACTAGT 1 cut(s) 1022
BfaI CTAG 6 cut(s) 108, 204, 255, 501, 1017, 1023
BfmI CTRYAG 1 cut(s) 81
BisI GCNGC 1 cut(s) 792
BlnI CCTAGG 1 cut(s) 1016
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 793
Bme1390I CCNGG 4 cut(s) 275, 509, 510, 995
Bme18I GGWCC 1 cut(s) 1228
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 1013, 1228
BmiI GGNNCC 3 cut(s) 242, 991, 1229
BmrFI CCNGG 4 cut(s) 275, 509, 510, 995
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 4 cut(s) 631, 1063, 1092, 1180
BmuI ACTGGG 1 cut(s) 958
Bpu1102I GCTNAGC 1 cut(s) 174
Bpu14I TTCGAA 1 cut(s) 1197
BpuMI CCSGG 4 cut(s) 275, 509, 510, 995
Bsa29I ATCGAT 1 cut(s) 1086
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 3 cut(s) 508, 993, 1016
BsaXI ACNNNNNCTCC 2 cut(s) 1146, 1176
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 986
Bse118I RCCGGY 1 cut(s) 1190
Bse1I ACTGG 2 cut(s) 632, 953
Bse3DI GCAATG 2 cut(s) 188, 1004
BseCI ATCGAT 1 cut(s) 1086
BseDI CCNNGG 3 cut(s) 508, 993, 1016
BseGI GGATG 4 cut(s) 154, 642, 661, 1154
BseLI CCNNNNNNNGG 2 cut(s) 280, 986
BseMI GCAATG 2 cut(s) 188, 1004
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 632, 953
BseRI GAGGAG 2 cut(s) 840, 1080
BseXI GCAGC 1 cut(s) 778
BshFI GGCC 1 cut(s) 1015
BshVI ATCGAT 1 cut(s) 1086
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 4 cut(s) 275, 509, 995, 1191
BslFI GGGAC 1 cut(s) 1241
BslI CCNNNNNNNGG 2 cut(s) 280, 986
BsmAI GTCTC 4 cut(s) 225, 374, 603, 662
BsmFI GGGAC 1 cut(s) 1241
BsnI GGCC 1 cut(s) 1015
Bso31I GGTCTC 1 cut(s) 603
BsoBI CYCGRG 1 cut(s) 508
Bsp119I TTCGAA 1 cut(s) 1197
Bsp1286I GDGCHC 1 cut(s) 328
Bsp1407I TGTACA 1 cut(s) 999
Bsp143I GATC 4 cut(s) 265, 289, 577, 709
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 3 cut(s) 768, 1028, 1178
BspANI GGCC 1 cut(s) 1015
BspCNI CTCAG 1 cut(s) 340
BspDI ATCGAT 1 cut(s) 1086
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 242, 991, 1229
BspPI GGATC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 1197
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 2 cut(s) 188, 1004
BsrFI RCCGGY 1 cut(s) 1190
BsrGI TGTACA 1 cut(s) 999
BsrI ACTGG 2 cut(s) 632, 953
BssAI RCCGGY 1 cut(s) 1190
BssECI CCNNGG 3 cut(s) 508, 993, 1016
BssMI GATC 4 cut(s) 265, 289, 577, 709
BssT1I CCWWGG 1 cut(s) 1016
Bst4CI ACNGT 5 cut(s) 346, 649, 785, 807, 973
Bst6I CTCTTC 2 cut(s) 411, 660
BstAUI TGTACA 1 cut(s) 999
BstBI TTCGAA 1 cut(s) 1197
BstDEI CTNAG 4 cut(s) 174, 194, 224, 327
BstF5I GGATG 4 cut(s) 154, 642, 661, 1154
BstKTI GATC 4 cut(s) 268, 292, 580, 712
BstMAI GTCTC 4 cut(s) 225, 374, 603, 662
BstMBI GATC 4 cut(s) 265, 289, 577, 709
BstMWI GCNNNNNNNGC 2 cut(s) 481, 800
BstSCI CCNGG 4 cut(s) 273, 507, 508, 993
BstSFI CTRYAG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 778
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 1086
BsuRI GGCC 1 cut(s) 1015
BsuTUI ATCGAT 1 cut(s) 1086
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 4 cut(s) 154, 642, 661, 1154
BtsI GCAGTG 1 cut(s) 1285
BtsIMutI CAGTG 4 cut(s) 351, 625, 645, 1285
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 1190
Cfr13I GGNCC 2 cut(s) 1013, 1228
Cfr9I CCCGGG 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 1086
Csp6I GTAC 4 cut(s) 104, 498, 1000, 1031
CviAII CATG 3 cut(s) 401, 680, 1290
CviJI RGCY 9 cut(s) 173, 178, 203, 326, 504, 760, 835, 1015, 1190
CviKI_1 RGCY 9 cut(s) 173, 178, 203, 326, 504, 760, 835, 1015, 1190
CviQI GTAC 4 cut(s) 104, 498, 1000, 1031
DdeI CTNAG 4 cut(s) 174, 194, 224, 327
DpnI GATC 4 cut(s) 267, 291, 579, 711
DpnII GATC 4 cut(s) 265, 289, 577, 709
Eam1104I CTCTTC 2 cut(s) 411, 660
EarI CTCTTC 2 cut(s) 411, 660
Eco130I CCWWGG 1 cut(s) 1016
Eco24I GRGCYC 1 cut(s) 328
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 1228
Eco88I CYCGRG 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 1013
EcoT14I CCWWGG 1 cut(s) 1016
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 1016
FaeI CATG 3 cut(s) 404, 683, 1293
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 1 cut(s) 1241
FatI CATG 3 cut(s) 400, 679, 1289
FauI CCCGC 2 cut(s) 775, 1171
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 1 cut(s) 792
FokI GGATG 4 cut(s) 161, 649, 668, 1161
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 792
FspBI CTAG 6 cut(s) 108, 204, 255, 501, 1017, 1023
GluI GCNGC 1 cut(s) 792
HaeIII GGCC 1 cut(s) 1015
HapII CCGG 4 cut(s) 275, 509, 995, 1191
Hin1II CATG 3 cut(s) 404, 683, 1293
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 4 cut(s) 58, 339, 589, 748
HpaII CCGG 4 cut(s) 275, 509, 995, 1191
HphI GGTGA 2 cut(s) 554, 1088
Hpy166II GTNNAC 5 cut(s) 1000, 1002, 1033, 1102, 1135
Hpy188I TCNGA 4 cut(s) 90, 603, 811, 912
Hpy188III TCNNGA 4 cut(s) 401, 575, 779, 822
Hpy8I GTNNAC 5 cut(s) 1000, 1002, 1033, 1102, 1135
Hpy99I CGWCG 1 cut(s) 985
HpyAV CCTTC 7 cut(s) 231, 355, 560, 824, 907, 1139, 1193
HpyCH4III ACNGT 5 cut(s) 346, 649, 785, 807, 973
HpyCH4V TGCA 6 cut(s) 232, 316, 851, 1054, 1083, 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 481, 800
HpyF3I CTNAG 4 cut(s) 174, 194, 224, 327
Hsp92II CATG 3 cut(s) 404, 683, 1293
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 265, 289, 577, 709
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 4 cut(s) 631, 1063, 1092, 1180
MaeI CTAG 6 cut(s) 108, 204, 255, 501, 1017, 1023
MaeIII GTNAC 6 cut(s) 70, 340, 407, 514, 737, 1265
MalI GATC 4 cut(s) 267, 291, 579, 711
MboI GATC 4 cut(s) 265, 289, 577, 709
MboII GAAGA 7 cut(s) 28, 120, 428, 616, 677, 716, 719
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 1 cut(s) 796
MlyI GAGTC 2 cut(s) 348, 742
MmeI TCCRAC 1 cut(s) 642
MseI TTAA 5 cut(s) 180, 366, 444, 882, 1113
MslI CAYNNNNRTG 1 cut(s) 1288
MspI CCGG 4 cut(s) 275, 509, 995, 1191
MspR9I CCNGG 4 cut(s) 275, 509, 510, 995
MwoI GCNNNNNNNGC 2 cut(s) 481, 800
NciI CCSGG 4 cut(s) 275, 509, 510, 995
NdeII GATC 4 cut(s) 265, 289, 577, 709
NlaIII CATG 3 cut(s) 404, 683, 1293
NlaIV GGNNCC 3 cut(s) 242, 991, 1229
NmuCI GTSAC 2 cut(s) 340, 514
NspV TTCGAA 1 cut(s) 1197
PagI TCATGA 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 977
PfeI GAWTC 2 cut(s) 58, 589
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 1 cut(s) 793
PleI GAGTC 2 cut(s) 347, 742
PpsI GAGTC 2 cut(s) 347, 742
PsiI TTATAA 1 cut(s) 1185
PspN4I GGNNCC 3 cut(s) 242, 991, 1229
PspPI GGNCC 2 cut(s) 1013, 1228
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 4 cut(s) 105, 499, 1001, 1032
RsaNI GTAC 4 cut(s) 104, 498, 1000, 1031
RseI CAYNNNNRTG 1 cut(s) 1288
SaqAI TTAA 5 cut(s) 180, 366, 444, 882, 1113
SatI GCNGC 1 cut(s) 792
Sau3AI GATC 4 cut(s) 265, 289, 577, 709
Sau96I GGNCC 2 cut(s) 1013, 1228
SchI GAGTC 2 cut(s) 348, 742
ScrFI CCNGG 4 cut(s) 275, 509, 510, 995
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 4 cut(s) 631, 1063, 1092, 1180
SfcI CTRYAG 1 cut(s) 81
SfuI TTCGAA 1 cut(s) 1197
SinI GGWCC 1 cut(s) 1228
SmaI CCCGGG 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 1288
SpeI ACTAGT 1 cut(s) 1022
Sse9I AATT 1 cut(s) 796
SsiI CCGC 3 cut(s) 768, 1028, 1178
SspI AATATT 1 cut(s) 904
SspMI CTAG 6 cut(s) 108, 204, 255, 501, 1017, 1023
StyD4I CCNGG 4 cut(s) 273, 507, 508, 993
StyI CCWWGG 1 cut(s) 1016
TaaI ACNGT 5 cut(s) 346, 649, 785, 807, 973
TaqI TCGA 4 cut(s) 337, 359, 1086, 1197
TaqII GACCGA 2 cut(s) 585, 1245
TasI AATT 1 cut(s) 796
TatI WGTACW 1 cut(s) 999
TfiI GAWTC 2 cut(s) 58, 589
Tru1I TTAA 5 cut(s) 180, 366, 444, 882, 1113
Tru9I TTAA 5 cut(s) 180, 366, 444, 882, 1113
TscAI CASTG 4 cut(s) 351, 632, 652, 1285
TseFI GTSAC 2 cut(s) 340, 514
TseI GCWGC 1 cut(s) 791
Tsp45I GTSAC 2 cut(s) 340, 514
TspDTI ATGAA 5 cut(s) 177, 437, 656, 688, 896
TspGWI ACGGA 1 cut(s) 278
TspMI CCCGGG 1 cut(s) 508
TspRI CASTG 4 cut(s) 351, 632, 652, 1285
VpaK11BI GGWCC 1 cut(s) 1228
XmaI CCCGGG 1 cut(s) 508
XmaJI CCTAGG 1 cut(s) 1016
XspI CTAG 6 cut(s) 108, 204, 255, 501, 1017, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.