Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013099

Physical Location & Seq

Genomic Coordinates Reverse (-)

9768166 .. 9769449

Gene Structure 1284 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1126500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCTATTATCAAGAAACAGCGTCTGAAGGACAAAGCGGCTTTTCGCCGTTTCTTGCCAGTGGTTTCGATTACATTGTTAGGGGTTTTCCTGCTGATGTTCCTCCTGAGATTTAATTCGAGTTCAAATTTTGTCACACCGCCTTCGGATGATTTTGATGAAGTATCAATAAGGAGTGGTAGTCGTTCACATGTTCGACAAATACTTAGCCTCCCTAAGCAGACTGAATTGTCAGTTCAGTTGGAAAAGAGAAATCAGATGCCCCCTAGGAACTTAGATCTCTATCCAAGTCTAGCCAAGGATCATATAACTATAGTTCTTTATGTGCACAACCGGCCGCAGTATCTCAAAGTAGCTGTTGATAGTCTGTCTCGGGTTGTAGGTATAAATGAAACTTTACTAATAGTTAGTCATGATGGATTCTTTGAAGAAATGAACAAGGTTGTGGAAGGGATCAGATTTTGCCAGGTGAAACAGATATTTGCTCCTTACTCGCCCCACGTCTTTCCCAATAGTTTTCCAGGGGCATCATCTACAGACTGTAAGGGAAAGGATGATGCGAAAGCGAAACACTGTAAGGGGACTCCTGATCAGTACGGAAACCACCGATCTCCAAAGATCGTGTCATTAAAGCATCATTGGTGGTGGATGATGAACACAGTGTGGGATGGATTGAAGGAGACCAAGGATCACAATGGTCATATTCTTTTCATAGAGGAGGACCATTATATATATCCCAATGCATATCGTAATTTGCAGATACTCACGGAATTGAAGCCTCATAAATGCCCTCATTGCTATGCTGCAAACTTAGCACCGTGTAATGTGAATGCAAAAGGAGAAGGTTGGGATAGTTTGATTGCAGAGAGAATGGGAAACGTGGGATATACATTTAACCGGACTGTTTGGAGGAAAATTCACAAGAAGTCTTCAGAATTTTGTTTCTTTGATGAATACAATTGGGATATAACAATGTGGGCAACAGTCTATCCCTCATTTGGTAATCCTGTTTTCACATTACGAGGGCCTCGGACTAGTGCAGTACACTTTGGTAAGTGTGGTTTGCACCAGGGACAGGGGGAGGCAAAAACATGTATTGACAACGGCATGGTGAACTTTGATGTGGAGGAGATAGATAAGGTTGCAAACATCAACTCGGACTGGGAAGTGCAAGTCTTTAAGAATCAGCCAGGGTACCAAGCCGGGTTCAAGGGTTGGGGAGGTTGGGGGGACAAGAGAGACCACCAATTGTGCTTGAGTTTTGCTCAGATGTATCATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0006464 GO:0006486 GO:0006487 GO:0006807 GO:0008150 GO:0008152 GO:0008194 GO:0008375 GO:0008455 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009311 GO:0009312 GO:0009987 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019538 GO:0030246 GO:0031090 GO:0031984 GO:0034645 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0070085 GO:0071704 GO:0098588 GO:0098791 GO:0140096 GO:0140103 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko00513 ko01100 map00510 map00513 map01100

Pfam Domains

Protein Families

MGAT2

Protein Analysis

428

Amino Acids

48.98

Weight (kDa)

8.84

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MGAT2	PF05060	87 - 418	6.6e-102	N-acetylglucosaminyltransferase II (MGAT2)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05320
fragaria_vesca	FvH4_6g40640
malus_domestica	MD09G1126500.v1.1 MD17G1116800.v1.1
prunus_persica	Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1 Prupe.3G198000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0156011
rosa_laevigata	RLG00000020881
rosa_multiflora	Rmu_sc0000260.1_g000023
rosa_roxburghii	Rroxscaffold_2G00093290
rosa_rugosa	Rorug02G0451200
rosa_samantha	Rh2AG518000 Rh2BG529200 Rh2CG502700 Rh2DG538300
rosa_wichuraiana	Rw2G042650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	229
Acc65I	GGTACC	1 cut(s)	1194
AccB1I	GGYRCC	1 cut(s)	1194
AciI	CCGC	3 cut(s)	38, 140, 338
AclWI	GGATC	3 cut(s)	309, 461, 696
AcoI	YGGCCR	1 cut(s)	335
AcsI	RAATTY	3 cut(s)	127, 915, 935
AcuI	CTGAAG	2 cut(s)	47, 915
AdeI	CACNNNGTG	1 cut(s)	663
AfaI	GTAC	3 cut(s)	596, 1044, 1196
AfiI	CCNNNNNNNGG	2 cut(s)	998, 1075
AflIII	ACRYGT	2 cut(s)	190, 1091
AgsI	TTSAA	5 cut(s)	126, 428, 676, 775, 1210
AhlI	ACTAGT	1 cut(s)	1034
AjiI	CACGTC	1 cut(s)	502
AjnI	CCWGG	4 cut(s)	465, 520, 1068, 1189
AluBI	AGCT	1 cut(s)	356
AluI	AGCT	1 cut(s)	356
Alw21I	GWGCWC	1 cut(s)	330
Alw26I	GTCTC	3 cut(s)	375, 674, 1233
Alw44I	GTGCAC	1 cut(s)	326
AlwI	GGATC	3 cut(s)	309, 461, 696
AlwNI	CAGNNNCTG	1 cut(s)	25
Ama87I	CYCGRG	1 cut(s)	372
AoxI	GGCC	2 cut(s)	335, 1025
ApaLI	GTGCAC	1 cut(s)	326
ApeKI	GCWGC	1 cut(s)	803
ApoI	RAATTY	3 cut(s)	127, 915, 935
ArsI	GACNNNNNNTTYG	2 cut(s)	608, 640
Asp718I	GGTACC	1 cut(s)	1194
AspA2I	CCTAGG	1 cut(s)	266
AspS9I	GGNCC	2 cut(s)	721, 1025
AsuC2I	CCSGG	1 cut(s)	1204
AsuHPI	GGTGA	2 cut(s)	481, 1123
AvaI	CYCGRG	1 cut(s)	372
AvaII	GGWCC	1 cut(s)	721
AvrII	CCTAGG	1 cut(s)	266
BaeGI	GKGCMC	1 cut(s)	330
BanI	GGYRCC	1 cut(s)	1194
BbsI	GAAGAC	1 cut(s)	921
Bbv12I	GWGCWC	1 cut(s)	330
BbvI	GCAGC	1 cut(s)	790
BccI	CCATC	2 cut(s)	410, 662
BceAI	ACGGC	2 cut(s)	33, 1120
BciT130I	CCWGG	4 cut(s)	467, 522, 1070, 1191
BclI	TGATCA	1 cut(s)	589
BcnI	CCSGG	1 cut(s)	1204
BcoDI	GTCTC	3 cut(s)	375, 674, 1233
BcuI	ACTAGT	1 cut(s)	1034
BfaI	CTAG	3 cut(s)	267, 293, 1035
BfmI	CTRYAG	2 cut(s)	312, 534
BglII	AGATCT	1 cut(s)	277
BisI	GCNGC	3 cut(s)	39, 338, 804
BlnI	CCTAGG	1 cut(s)	266
BlsI	GCNGC	3 cut(s)	40, 339, 805
Bme1390I	CCNGG	5 cut(s)	467, 522, 1070, 1191, 1204
Bme18I	GGWCC	1 cut(s)	721
BmeT110I	CYCGRG	1 cut(s)	372
BmgBI	CACGTC	1 cut(s)	502
BmgT120I	GGNCC	2 cut(s)	721, 1025
BmiI	GGNNCC	1 cut(s)	1196
BmrFI	CCNGG	5 cut(s)	467, 522, 1070, 1191, 1204
BmrI	ACTGGG	1 cut(s)	1171
BmsI	GCATC	4 cut(s)	249, 536, 547, 643
BmuI	ACTGGG	1 cut(s)	1171
BpiI	GAAGAC	1 cut(s)	921
BplI	GAGNNNNNCTC	2 cut(s)	1249, 1281
Bpu10I	CCTNAGC	1 cut(s)	216
BpuEI	CTTGAG	1 cut(s)	1276
BpuMI	CCSGG	1 cut(s)	1204
BsaBI	GATNNNNATC	1 cut(s)	282
BsaI	GGTCTC	2 cut(s)	674, 1233
BsaJI	CCNNGG	7 cut(s)	266, 297, 521, 684, 1028, 1069, 1190
BsaWI	WCCGGW	1 cut(s)	897
BsaXI	ACNNNNNCTCC	4 cut(s)	166, 195, 196, 225
Bsc4I	CCNNNNNNNGG	2 cut(s)	998, 1075
Bse118I	RCCGGY	1 cut(s)	333
Bse1I	ACTGG	2 cut(s)	59, 1166
Bse3DI	GCAATG	1 cut(s)	793
Bse8I	GATNNNNATC	1 cut(s)	282
BseBI	CCWGG	4 cut(s)	467, 522, 1070, 1191
BseDI	CCNNGG	7 cut(s)	266, 297, 521, 684, 1028, 1069, 1190
BseGI	GGATG	4 cut(s)	154, 559, 654, 673
BseJI	GATNNNNATC	1 cut(s)	282
BseLI	CCNNNNNNNGG	2 cut(s)	998, 1075
BseMI	GCAATG	1 cut(s)	793
BseMII	CTCAG	2 cut(s)	98, 1280
BseNI	ACTGG	2 cut(s)	59, 1166
BseRI	GAGGAG	2 cut(s)	731, 1142
BseSI	GKGCMC	1 cut(s)	330
BseX3I	CGGCCG	1 cut(s)	335
BseXI	GCAGC	1 cut(s)	790
BsgI	GTGCAG	1 cut(s)	1059
Bsh1285I	CGRYCG	1 cut(s)	338
BshFI	GGCC	2 cut(s)	337, 1027
BshNI	GGYRCC	1 cut(s)	1194
BsiEI	CGRYCG	1 cut(s)	338
BsiHKAI	GWGCWC	1 cut(s)	330
BsiHKCI	CYCGRG	1 cut(s)	372
BsiSI	CCGG	3 cut(s)	334, 898, 1203
BslFI	GGGAC	3 cut(s)	595, 1086, 1244
BslI	CCNNNNNNNGG	2 cut(s)	998, 1075
BsmAI	GTCTC	3 cut(s)	375, 674, 1233
BsmFI	GGGAC	3 cut(s)	595, 1086, 1244
BsmI	GAATGC	1 cut(s)	835
BsnI	GGCC	2 cut(s)	337, 1027
Bso31I	GGTCTC	2 cut(s)	674, 1233
BsoBI	CYCGRG	1 cut(s)	372
Bsp1286I	GDGCHC	1 cut(s)	330
Bsp143I	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
BspACI	CCGC	3 cut(s)	38, 140, 338
BspANI	GGCC	2 cut(s)	337, 1027
BspCNI	CTCAG	2 cut(s)	99, 1279
BspHI	TCATGA	1 cut(s)	412
BspLI	GGNNCC	1 cut(s)	1196
BspPI	GGATC	3 cut(s)	309, 461, 696
BspT107I	GGYRCC	1 cut(s)	1194
BspTNI	GGTCTC	2 cut(s)	674, 1233
BsrDI	GCAATG	1 cut(s)	793
BsrFI	RCCGGY	1 cut(s)	333
BsrI	ACTGG	2 cut(s)	59, 1166
BssAI	RCCGGY	1 cut(s)	333
BssECI	CCNNGG	7 cut(s)	266, 297, 521, 684, 1028, 1069, 1190
BssMI	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
BssT1I	CCWWGG	3 cut(s)	266, 297, 684
Bst2UI	CCWGG	4 cut(s)	467, 522, 1070, 1191
Bst4CI	ACNGT	6 cut(s)	542, 575, 661, 819, 904, 985
BstDEI	CTNAG	6 cut(s)	107, 206, 216, 274, 811, 1266
BstF5I	GGATG	4 cut(s)	154, 559, 654, 673
BstKTI	GATC	7 cut(s)	280, 304, 456, 592, 611, 621, 691
BstMAI	GTCTC	3 cut(s)	375, 674, 1233
BstMBI	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
BstMCI	CGRYCG	1 cut(s)	338
BstMWI	GCNNNNNNNGC	3 cut(s)	334, 795, 812
BstNI	CCWGG	4 cut(s)	467, 522, 1070, 1191
BstNSI	RCATGY	2 cut(s)	194, 1095
BstSCI	CCNGG	5 cut(s)	465, 520, 1068, 1189, 1202
BstSFI	CTRYAG	2 cut(s)	312, 534
BstSLI	GKGCMC	1 cut(s)	330
BstV1I	GCAGC	1 cut(s)	790
BstV2I	GAAGAC	1 cut(s)	921
BstX2I	RGATCY	1 cut(s)	277
BstYI	RGATCY	1 cut(s)	277
BstZI	CGGCCG	1 cut(s)	335
BsuRI	GGCC	2 cut(s)	337, 1027
BtrI	CACGTC	1 cut(s)	502
BtsCI	GGATG	4 cut(s)	154, 559, 654, 673
BtsIMutI	CAGTG	3 cut(s)	66, 571, 666
CaiI	CAGNNNCTG	1 cut(s)	25
CciI	TCATGA	1 cut(s)	412
Cfr10I	RCCGGY	1 cut(s)	333
Cfr13I	GGNCC	2 cut(s)	721, 1025
CseI	GACGC	1 cut(s)	11
Csp6I	GTAC	3 cut(s)	595, 1043, 1195
CviAII	CATG	4 cut(s)	191, 413, 1092, 1108
CviQI	GTAC	3 cut(s)	595, 1043, 1195
DdeI	CTNAG	6 cut(s)	107, 206, 216, 274, 811, 1266
DpnI	GATC	7 cut(s)	279, 303, 455, 591, 610, 620, 690
DpnII	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
DraIII	CACNNNGTG	1 cut(s)	663
DrdI	GACNNNNNNGTC	1 cut(s)	229
DseDI	GACNNNNNNGTC	1 cut(s)	229
EaeI	YGGCCR	1 cut(s)	335
EagI	CGGCCG	1 cut(s)	335
EclXI	CGGCCG	1 cut(s)	335
Eco130I	CCWWGG	3 cut(s)	266, 297, 684
Eco31I	GGTCTC	2 cut(s)	674, 1233
Eco47I	GGWCC	1 cut(s)	721
Eco52I	CGGCCG	1 cut(s)	335
Eco57I	CTGAAG	2 cut(s)	47, 915
Eco88I	CYCGRG	1 cut(s)	372
EcoO109I	RGGNCCY	1 cut(s)	1025
EcoRII	CCWGG	4 cut(s)	465, 520, 1068, 1189
EcoT14I	CCWWGG	3 cut(s)	266, 297, 684
EcoT22I	ATGCAT	1 cut(s)	745
ErhI	CCWWGG	3 cut(s)	266, 297, 684
FaeI	CATG	4 cut(s)	194, 416, 1095, 1111
FaqI	GGGAC	3 cut(s)	595, 1086, 1244
FatI	CATG	4 cut(s)	190, 412, 1091, 1107
FbaI	TGATCA	1 cut(s)	589
Fnu4HI	GCNGC	3 cut(s)	39, 338, 804
FokI	GGATG	4 cut(s)	161, 566, 661, 680
Fsp4HI	GCNGC	3 cut(s)	39, 338, 804
FspBI	CTAG	3 cut(s)	267, 293, 1035
GluI	GCNGC	3 cut(s)	39, 338, 804
HaeIII	GGCC	2 cut(s)	337, 1027
HapII	CCGG	3 cut(s)	334, 898, 1203
HgaI	GACGC	1 cut(s)	11
Hin1II	CATG	4 cut(s)	194, 416, 1095, 1111
HinfI	GANTC	3 cut(s)	420, 583, 1183
HpaII	CCGG	3 cut(s)	334, 898, 1203
HphI	GGTGA	2 cut(s)	481, 1123
Hpy166II	GTNNAC	4 cut(s)	188, 328, 1045, 1114
Hpy188I	TCNGA	8 cut(s)	27, 148, 258, 458, 934, 1032, 1159, 1269
Hpy188III	TCNNGA	4 cut(s)	13, 106, 413, 587
Hpy8I	GTNNAC	4 cut(s)	188, 328, 1045, 1114
HpyAV	CCTTC	5 cut(s)	22, 153, 443, 670, 836
HpyCH4III	ACNGT	6 cut(s)	542, 575, 661, 819, 904, 985
HpyCH4IV	ACGT	2 cut(s)	501, 879
HpyF10VI	GCNNNNNNNGC	3 cut(s)	334, 795, 812
HpyF3I	CTNAG	6 cut(s)	107, 206, 216, 274, 811, 1266
HpySE526I	ACGT	2 cut(s)	501, 879
Hsp92II	CATG	4 cut(s)	194, 416, 1095, 1111
KpnI	GGTACC	1 cut(s)	1198
Ksp22I	TGATCA	1 cut(s)	589
Kzo9I	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
LmnI	GCTCC	1 cut(s)	490
Lsp1109I	GCAGC	1 cut(s)	790
LweI	GCATC	4 cut(s)	249, 536, 547, 643
MaeI	CTAG	3 cut(s)	267, 293, 1035
MaeII	ACGT	2 cut(s)	501, 879
MaeIII	GTNAC	1 cut(s)	133
MalI	GATC	7 cut(s)	279, 303, 455, 591, 610, 620, 690
MboI	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
MboII	GAAGA	2 cut(s)	440, 921
MfeI	CAATTG	2 cut(s)	958, 1247
MflI	RGATCY	1 cut(s)	277
MhlI	GDGCHC	1 cut(s)	330
MluCI	AATT	9 cut(s)	115, 127, 227, 751, 770, 915, 935, 958, 1247
MlyI	GAGTC	1 cut(s)	577
MmeI	TCCRAC	1 cut(s)	222
Mph1103I	ATGCAT	1 cut(s)	745
MseI	TTAA	4 cut(s)	114, 629, 894, 1179
MslI	CAYNNNNRTG	1 cut(s)	798
MspI	CCGG	3 cut(s)	334, 898, 1203
MspR9I	CCNGG	5 cut(s)	467, 522, 1070, 1191, 1204
MunI	CAATTG	2 cut(s)	958, 1247
Mva1269I	GAATGC	1 cut(s)	835
MvaI	CCWGG	4 cut(s)	467, 522, 1070, 1191
MwoI	GCNNNNNNNGC	3 cut(s)	334, 795, 812
NciI	CCSGG	1 cut(s)	1204
NdeII	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
NlaIII	CATG	4 cut(s)	194, 416, 1095, 1111
NlaIV	GGNNCC	1 cut(s)	1196
NmuCI	GTSAC	1 cut(s)	133
NsiI	ATGCAT	1 cut(s)	745
NspI	RCATGY	2 cut(s)	194, 1095
PagI	TCATGA	1 cut(s)	412
PciI	ACATGT	2 cut(s)	190, 1091
PctI	GAATGC	1 cut(s)	835
PfeI	GAWTC	2 cut(s)	420, 1183
PkrI	GCNGC	3 cut(s)	40, 339, 805
PleI	GAGTC	1 cut(s)	577
PpsI	GAGTC	1 cut(s)	577
PscI	ACATGT	2 cut(s)	190, 1091
Psp6I	CCWGG	4 cut(s)	465, 520, 1068, 1189
PspGI	CCWGG	4 cut(s)	465, 520, 1068, 1189
PspN4I	GGNNCC	1 cut(s)	1196
PspPI	GGNCC	2 cut(s)	721, 1025
PstNI	CAGNNNCTG	1 cut(s)	25
PsuI	RGATCY	1 cut(s)	277
RsaI	GTAC	3 cut(s)	596, 1044, 1196
RsaNI	GTAC	3 cut(s)	595, 1043, 1195
RseI	CAYNNNNRTG	1 cut(s)	798
SaqAI	TTAA	4 cut(s)	114, 629, 894, 1179
SatI	GCNGC	3 cut(s)	39, 338, 804
Sau3AI	GATC	7 cut(s)	277, 301, 453, 589, 608, 618, 688
Sau96I	GGNCC	2 cut(s)	721, 1025
SchI	GAGTC	1 cut(s)	577
ScrFI	CCNGG	5 cut(s)	467, 522, 1070, 1191, 1204
SduI	GDGCHC	1 cut(s)	330
SetI	ASST	9 cut(s)	358, 385, 444, 471, 504, 847, 882, 1143, 1225
SfaNI	GCATC	4 cut(s)	249, 536, 547, 643
SfcI	CTRYAG	2 cut(s)	312, 534
SinI	GGWCC	1 cut(s)	721
SmiMI	CAYNNNNRTG	1 cut(s)	798
SmlI	CTYRAG	1 cut(s)	1255
SmoI	CTYRAG	1 cut(s)	1255
SpeI	ACTAGT	1 cut(s)	1034
Sse9I	AATT	9 cut(s)	115, 127, 227, 751, 770, 915, 935, 958, 1247
SsiI	CCGC	3 cut(s)	38, 140, 338
SspMI	CTAG	3 cut(s)	267, 293, 1035
StyD4I	CCNGG	5 cut(s)	465, 520, 1068, 1189, 1202
StyI	CCWWGG	3 cut(s)	266, 297, 684
TaaI	ACNGT	6 cut(s)	542, 575, 661, 819, 904, 985
TaiI	ACGT	2 cut(s)	504, 882
TaqI	TCGA	3 cut(s)	68, 119, 196
TasI	AATT	9 cut(s)	115, 127, 227, 751, 770, 915, 935, 958, 1247
TatI	WGTACW	1 cut(s)	1042
TauI	GCSGC	2 cut(s)	41, 340
TfiI	GAWTC	2 cut(s)	420, 1183
Tru1I	TTAA	4 cut(s)	114, 629, 894, 1179
Tru9I	TTAA	4 cut(s)	114, 629, 894, 1179
TscAI	CASTG	3 cut(s)	66, 578, 666
TseFI	GTSAC	1 cut(s)	133
TseI	GCWGC	1 cut(s)	803
Tsp45I	GTSAC	1 cut(s)	133
TspDTI	ATGAA	7 cut(s)	174, 405, 449, 668, 700, 966, 1269
TspGWI	ACGGA	2 cut(s)	612, 782
TspRI	CASTG	3 cut(s)	66, 578, 666
VneI	GTGCAC	1 cut(s)	326
VpaK11BI	GGWCC	1 cut(s)	721
XapI	RAATTY	3 cut(s)	127, 915, 935
XceI	RCATGY	2 cut(s)	194, 1095
XmaJI	CCTAGG	1 cut(s)	266
XspI	CTAG	3 cut(s)	267, 293, 1035
Zsp2I	ATGCAT	1 cut(s)	745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.