AT2G30550

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009430
Physical Location & Seq
Forward (+)
13014530 .. 13017329
2800 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30550.2

Sequence Viewer

Length: 1395 bp
ATGGCGGCTATTCCTTCCCACAACAACCTTCTTACCATCAACCACAAAAACTCCATAACCGGTTCTTCTTCCCTAAATACCAATTTCTCAGAAATCAATTTTCCCGCCAAATTCCGAGTAGCTACGAGAGCTTTGTCCAGAACCGACGAGTCGTCTTTATCCGCCGTGATTTCTCGCCTCGAGCGAGAAAGGCGGGAAAGACAAGGTTTATTAATCGAGGAAGCGGAAGGAGCTGGAGAACTATGGATGACGGCGGAAGATATTCGCCGGCGAGATAAAAAAACCGAAGAAGAAAGAAGACTAAGAGACACGTGGCGTAAGATCCAAGGAGAAGACGATTGGGCCGGGTTAATGGATCCAATGGATCCAATTCTTAGATCGGAGCTAATCCGTTACGGCGAAATGGCTCAAGCTTGTTACGACGCTTTCGATTTCGATCCCGCTTCCAAATACTGCGGCACCTCCAGGTTCACGCGACTCGAGTTCTTCGATTCTCTCGGAATGATCGATTCCGGTTACGAGGTGGCGCGTTACCTCTACGCGACGTCGAACATCAATCTCCCGAACTTCTTCTCGAAATCGCGGTGGTCTAAAGTCTGGAGCAAAAACGCTAATTGGATGGGATACGTCGCCGTTTCAGACGACGAAACGTCTCGTAACCGACTCGGCCGCCGTGATATCGCGATTGCGTGGAGAGGAACCGTTACGAAACTTGAATGGATCGCGGATCTAAAGGATTATTTAAAACCGGTAACCGAAAACAAGATCCGATGCCCCGACCCGGCCGTTAAAGTCGAATCCGGATTCTTAGATCTCTACACTGACAAAGACACAACCTGCAAATTCGCGAGATTCTCAGCGCGTGAACAGATTTTAACGGAGGTGAAACGGTTAGTGGAAGAACACGGCGACGACGATGATTCCGATTTAAGCATCACCGTGACGGGACACAGTCTCGGCGGCGCGTTAGCGATATTAAGCGCGTACGATATAGCGGAGATGAGATTGAATCGGAGTAAGAAAGGGAAAGTGATTCCGGTGACGGTGTTGACATACGGAGGACCGAGAGTTGGGAACGTTAGGTTTAGGGAGAGGATGGAGGAATTGGGAGTGAAAGTGATGAGAGTAGTGAATGTTCACGACGTGGTTCCCAAGTCGCCGGGATTGTTTTTGAACGAGAGTAGACCTCACGCGCTGATGAAGATAGCGGAGGGGTTGCCGTGGTGTTATAGCCACGTGGGGGAGGAGCTGGCGTTGGATCATCAGAACTCGCCGTTTCTTAAACCTTCCGTTGATGTTTCTACTGCTCATAATCTTGAAGCTATGCTTCATTTACTTGACGGGTACGTTTCCTTTTTATTTTACTTTTCATTGTTTATATATTCATGTTCATAA

Protein Analysis

464

Amino Acids

52.8

Weight (kDa)

6.13

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 229 - 389 5.3e-40 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 458
AccI GTMKAC 1 cut(s) 1183
AccIII TCCGGA 1 cut(s) 800
AclI AACGTT 1 cut(s) 1077
AcoI YGGCCR 2 cut(s) 667, 783
AcsI RAATTY 2 cut(s) 110, 842
AcvI CACGTG 2 cut(s) 312, 1237
AcyI GRCGYC 1 cut(s) 545
AdeI CACNNNGTG 1 cut(s) 1144
AfaI GTAC 2 cut(s) 986, 1346
AfiI CCNNNNNNNGG 3 cut(s) 781, 1070, 1240
AflIII ACRYGT 1 cut(s) 309
AgeI ACCGGT 2 cut(s) 59, 748
AgsI TTSAA 4 cut(s) 716, 1009, 1174, 1319
AhdI GACNNNNNGTC 2 cut(s) 151, 649
AjiI CACGTC 1 cut(s) 1144
AjnI CCWGG 1 cut(s) 464
AluBI AGCT 7 cut(s) 122, 131, 233, 385, 413, 1249, 1322
AluI AGCT 7 cut(s) 122, 131, 233, 385, 413, 1249, 1322
Alw26I GTCTC 3 cut(s) 300, 657, 959
Ama87I CYCGRG 2 cut(s) 179, 479
Aor13HI TCCGGA 1 cut(s) 800
AoxI GGCC 3 cut(s) 342, 667, 783
ApoI RAATTY 2 cut(s) 110, 842
AseI ATTAAT 1 cut(s) 212
AsiGI ACCGGT 2 cut(s) 59, 748
Asp700I GAANNNNTTC 2 cut(s) 261, 569
AspLEI GCGC 5 cut(s) 529, 862, 965, 983, 1195
AspS9I GGNCC 2 cut(s) 342, 1061
AsuC2I CCSGG 3 cut(s) 346, 782, 1161
AsuHPI GGTGA 3 cut(s) 895, 928, 1051
AvaI CYCGRG 2 cut(s) 179, 479
AvaII GGWCC 1 cut(s) 1061
BamHI GGATCC 2 cut(s) 355, 364
BanI GGYRCC 1 cut(s) 458
BbrPI CACGTG 2 cut(s) 312, 1237
BbsI GAAGAC 2 cut(s) 304, 339
BccI CCATC 3 cut(s) 44, 613, 1090
BceAI ACGGC 9 cut(s) 149, 267, 412, 617, 657, 770, 922, 1204, 1258
BciT130I CCWGG 1 cut(s) 466
BciVI GTATCC 1 cut(s) 617
BcnI CCSGG 3 cut(s) 346, 782, 1161
BcoDI GTCTC 3 cut(s) 300, 657, 959
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 617
BglII AGATCT 1 cut(s) 811
BisI GCNGC 4 cut(s) 6, 457, 670, 961
BlsI GCNGC 4 cut(s) 7, 458, 671, 962
Bme1390I CCNGG 4 cut(s) 346, 466, 782, 1161
Bme18I GGWCC 1 cut(s) 1061
BmeRI GACNNNNNGTC 2 cut(s) 151, 649
BmeT110I CYCGRG 2 cut(s) 179, 479
BmgBI CACGTC 1 cut(s) 1144
BmgT120I GGNCC 2 cut(s) 342, 1061
BmiI GGNNCC 5 cut(s) 357, 366, 460, 700, 1149
BmrFI CCNGG 4 cut(s) 346, 466, 782, 1161
BmsI GCATC 2 cut(s) 761, 942
BpiI GAAGAC 2 cut(s) 304, 339
BplI GAGNNNNNCTC 2 cut(s) 1171, 1203
BpmI CTGGAG 3 cut(s) 255, 448, 619
BpuEI CTTGAG 1 cut(s) 393
BpuMI CCSGG 3 cut(s) 346, 782, 1161
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 2 cut(s) 312, 1237
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 2 cut(s) 325, 1220
BsaWI WCCGGW 5 cut(s) 59, 512, 748, 800, 1036
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 1101, 1131, 1238, 1268
Bsc4I CCNNNNNNNGG 3 cut(s) 781, 1070, 1240
Bse118I RCCGGY 3 cut(s) 59, 267, 748
Bse8I GATNNNNATC 1 cut(s) 435
BseAI TCCGGA 1 cut(s) 800
BseBI CCWGG 1 cut(s) 466
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 325, 1220
BseGI GGATG 3 cut(s) 252, 624, 1101
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 3 cut(s) 781, 1070, 1240
BseMII CTCAG 2 cut(s) 102, 870
BseRI GAGGAG 1 cut(s) 1259
BseX3I CGGCCG 2 cut(s) 667, 783
Bsh1285I CGRYCG 2 cut(s) 670, 786
BshFI GGCC 3 cut(s) 344, 669, 785
BshNI GGYRCC 1 cut(s) 458
BshTI ACCGGT 2 cut(s) 59, 748
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 2 cut(s) 670, 786
BsiHKCI CYCGRG 2 cut(s) 179, 479
BsiSI CCGG 9 cut(s) 60, 268, 345, 513, 749, 782, 801, 1037, 1160
BsiWI CGTACG 1 cut(s) 984
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 3 cut(s) 781, 1070, 1240
BsmAI GTCTC 3 cut(s) 300, 657, 959
BsmBI CGTCTC 1 cut(s) 657
BsmFI GGGAC 1 cut(s) 960
BsnI GGCC 3 cut(s) 344, 669, 785
BsoBI CYCGRG 2 cut(s) 179, 479
Bsp13I TCCGGA 1 cut(s) 800
Bsp68I TCGCGA 2 cut(s) 683, 848
BspANI GGCC 3 cut(s) 344, 669, 785
BspCNI CTCAG 2 cut(s) 101, 869
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 1 cut(s) 800
BspLI GGNNCC 5 cut(s) 357, 366, 460, 700, 1149
BspMI ACCTGC 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 458
BsrFI RCCGGY 3 cut(s) 59, 267, 748
BssAI RCCGGY 3 cut(s) 59, 267, 748
BssECI CCNNGG 2 cut(s) 325, 1220
BssNI GRCGYC 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 5 cut(s) 703, 891, 940, 953, 1045
BstACI GRCGYC 1 cut(s) 545
BstBAI YACGTR 2 cut(s) 312, 1237
BstC8I GCNNGC 2 cut(s) 269, 1251
BstDEI CTNAG 5 cut(s) 88, 302, 374, 808, 856
BstDSI CCRYGG 1 cut(s) 1220
BstEII GGTNACC 1 cut(s) 751
BstF5I GGATG 3 cut(s) 252, 624, 1101
BstHHI GCGC 5 cut(s) 529, 862, 965, 983, 1195
BstMAI GTCTC 3 cut(s) 300, 657, 959
BstMCI CGRYCG 2 cut(s) 670, 786
BstMWI GCNNNNNNNGC 3 cut(s) 128, 190, 230
BstNI CCWGG 1 cut(s) 466
BstPI GGTNACC 1 cut(s) 751
BstSCI CCNGG 4 cut(s) 344, 464, 780, 1159
BstV2I GAAGAC 2 cut(s) 304, 339
BstX2I RGATCY 6 cut(s) 321, 355, 364, 727, 765, 811
BstYI RGATCY 6 cut(s) 321, 355, 364, 727, 765, 811
BstZI CGGCCG 2 cut(s) 667, 783
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 1 cut(s) 617
BsuRI GGCC 3 cut(s) 344, 669, 785
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 1220
BtrI CACGTC 1 cut(s) 1144
BtsCI GGATG 3 cut(s) 252, 624, 1101
BtsIMutI CAGTG 1 cut(s) 819
BtuMI TCGCGA 2 cut(s) 683, 848
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 269, 1251
CfoI GCGC 5 cut(s) 529, 862, 965, 983, 1195
Cfr10I RCCGGY 3 cut(s) 59, 267, 748
Cfr13I GGNCC 2 cut(s) 342, 1061
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 985, 1345
CspAI ACCGGT 2 cut(s) 59, 748
CviAII CATG 1 cut(s) 1386
CviQI GTAC 2 cut(s) 985, 1345
DdeI CTNAG 5 cut(s) 88, 302, 374, 808, 856
DraI TTTAAA 1 cut(s) 744
DraIII CACNNNGTG 1 cut(s) 1144
DriI GACNNNNNGTC 2 cut(s) 151, 649
EaeI YGGCCR 2 cut(s) 667, 783
EagI CGGCCG 2 cut(s) 667, 783
Eam1105I GACNNNNNGTC 2 cut(s) 151, 649
EciI GGCGGA 2 cut(s) 151, 269
EclXI CGGCCG 2 cut(s) 667, 783
Eco130I CCWWGG 1 cut(s) 325
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 1061
Eco52I CGGCCG 2 cut(s) 667, 783
Eco72I CACGTG 2 cut(s) 312, 1237
Eco88I CYCGRG 2 cut(s) 179, 479
Eco91I GGTNACC 1 cut(s) 751
EcoO65I GGTNACC 1 cut(s) 751
EcoRII CCWGG 1 cut(s) 464
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
Esp3I CGTCTC 1 cut(s) 657
FaeI CATG 1 cut(s) 1389
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FaqI GGGAC 1 cut(s) 960
FatI CATG 1 cut(s) 1385
FauI CCCGC 3 cut(s) 112, 186, 448
FblI GTMKAC 1 cut(s) 1183
Fnu4HI GCNGC 4 cut(s) 6, 457, 670, 961
FokI GGATG 3 cut(s) 259, 631, 1108
Fsp4HI GCNGC 4 cut(s) 6, 457, 670, 961
GlaI GCGC 5 cut(s) 528, 861, 964, 982, 1194
GluI GCNGC 4 cut(s) 6, 457, 670, 961
GsuI CTGGAG 3 cut(s) 255, 448, 619
HaeIII GGCC 3 cut(s) 344, 669, 785
HapII CCGG 9 cut(s) 60, 268, 345, 513, 749, 782, 801, 1037, 1160
HgaI GACGC 1 cut(s) 431
HhaI GCGC 5 cut(s) 529, 862, 965, 983, 1195
Hin1I GRCGYC 1 cut(s) 545
Hin1II CATG 1 cut(s) 1389
Hin6I GCGC 5 cut(s) 527, 860, 963, 981, 1193
HinP1I GCGC 5 cut(s) 527, 860, 963, 981, 1193
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 1 cut(s) 411
HpaII CCGG 9 cut(s) 60, 268, 345, 513, 749, 782, 801, 1037, 1160
HphI GGTGA 3 cut(s) 895, 928, 1051
Hpy166II GTNNAC 5 cut(s) 471, 866, 1050, 1138, 1184
Hpy188I TCNGA 9 cut(s) 91, 116, 382, 500, 640, 770, 925, 1014, 1266
Hpy188III TCNNGA 9 cut(s) 138, 562, 574, 598, 682, 801, 847, 1139, 1316
Hpy8I GTNNAC 5 cut(s) 471, 866, 1050, 1138, 1184
Hpy99I CGWCG 9 cut(s) 149, 425, 547, 550, 632, 647, 914, 917, 1145
HpyAV CCTTC 4 cut(s) 24, 38, 221, 1296
HpyCH4III ACNGT 5 cut(s) 703, 891, 940, 953, 1045
HpyCH4IV ACGT 8 cut(s) 311, 545, 627, 650, 1077, 1143, 1236, 1347
HpyCH4V TGCA 1 cut(s) 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 190, 230
HpyF3I CTNAG 5 cut(s) 88, 302, 374, 808, 856
HpySE526I ACGT 8 cut(s) 311, 545, 627, 650, 1077, 1143, 1236, 1347
Hsp92I GRCGYC 1 cut(s) 545
Hsp92II CATG 1 cut(s) 1389
HspAI GCGC 5 cut(s) 527, 860, 963, 981, 1193
Kpn2I TCCGGA 1 cut(s) 800
KroI GCCGGC 1 cut(s) 267
KroNI GCCGGC 1 cut(s) 269
LmnI GCTCC 4 cut(s) 230, 382, 600, 1246
LweI GCATC 2 cut(s) 761, 942
MaeII ACGT 8 cut(s) 311, 545, 627, 650, 1077, 1143, 1236, 1347
MaeIII GTNAC 9 cut(s) 392, 416, 515, 530, 656, 703, 751, 940, 1039
MflI RGATCY 6 cut(s) 321, 355, 364, 727, 765, 811
MluCI AATT 7 cut(s) 82, 97, 110, 369, 613, 842, 1103
MlyI GAGTC 3 cut(s) 158, 471, 657
MmeI TCCRAC 1 cut(s) 1236
MreI CGCCGGCG 1 cut(s) 267
MroI TCCGGA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 267
MroXI GAANNNNTTC 2 cut(s) 261, 569
MseI TTAA 8 cut(s) 212, 350, 743, 789, 875, 929, 977, 1281
MslI CAYNNNNRTG 1 cut(s) 938
MspI CCGG 9 cut(s) 60, 268, 345, 513, 749, 782, 801, 1037, 1160
MspR9I CCNGG 4 cut(s) 346, 466, 782, 1161
MvaI CCWGG 1 cut(s) 466
MwoI GCNNNNNNNGC 3 cut(s) 128, 190, 230
NaeI GCCGGC 1 cut(s) 269
NciI CCSGG 3 cut(s) 346, 782, 1161
NgoMIV GCCGGC 1 cut(s) 267
NlaIII CATG 1 cut(s) 1389
NlaIV GGNNCC 5 cut(s) 357, 366, 460, 700, 1149
NmeAIII GCCGAG 2 cut(s) 645, 936
NmuCI GTSAC 2 cut(s) 940, 1039
NruI TCGCGA 2 cut(s) 683, 848
PaeR7I CTCGAG 2 cut(s) 179, 479
PcsI WCGNNNNNNNCGW 6 cut(s) 181, 426, 486, 504, 912, 921
PdiI GCCGGC 1 cut(s) 269
PdmI GAANNNNTTC 2 cut(s) 261, 569
PfeI GAWTC 8 cut(s) 491, 509, 797, 804, 852, 920, 1009, 1033
Pfl23II CGTACG 1 cut(s) 984
PflFI GACNNNGTC 1 cut(s) 951
PinAI ACCGGT 2 cut(s) 59, 748
PkrI GCNGC 4 cut(s) 7, 458, 671, 962
PleI GAGTC 3 cut(s) 157, 471, 657
PmaCI CACGTG 2 cut(s) 312, 1237
PmlI CACGTG 2 cut(s) 312, 1237
PpsI GAGTC 3 cut(s) 157, 471, 657
Ppu21I YACGTR 2 cut(s) 312, 1237
PshBI ATTAAT 1 cut(s) 212
Psp1406I AACGTT 1 cut(s) 1077
Psp6I CCWGG 1 cut(s) 464
PspCI CACGTG 2 cut(s) 312, 1237
PspEI GGTNACC 1 cut(s) 751
PspGI CCWGG 1 cut(s) 464
PspLI CGTACG 1 cut(s) 984
PspN4I GGNNCC 5 cut(s) 357, 366, 460, 700, 1149
PspPI GGNCC 2 cut(s) 342, 1061
PspXI VCTCGAGB 2 cut(s) 179, 479
PsrI GAACNNNNNNTAC 2 cut(s) 1119, 1151
PsuI RGATCY 6 cut(s) 321, 355, 364, 727, 765, 811
PsyI GACNNNGTC 1 cut(s) 951
RruI TCGCGA 2 cut(s) 683, 848
RsaI GTAC 2 cut(s) 986, 1346
RsaNI GTAC 2 cut(s) 985, 1345
RseI CAYNNNNRTG 1 cut(s) 938
SaqAI TTAA 8 cut(s) 212, 350, 743, 789, 875, 929, 977, 1281
SatI GCNGC 4 cut(s) 6, 457, 670, 961
Sau96I GGNCC 2 cut(s) 342, 1061
SchI GAGTC 3 cut(s) 158, 471, 657
ScrFI CCNGG 4 cut(s) 346, 466, 782, 1161
SfaNI GCATC 2 cut(s) 761, 942
Sfr274I CTCGAG 2 cut(s) 179, 479
SgrAI CRCCGGYG 1 cut(s) 267
SinI GGWCC 1 cut(s) 1061
SlaI CTCGAG 2 cut(s) 179, 479
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 179, 408, 479
SmoI CTYRAG 3 cut(s) 179, 408, 479
Sse9I AATT 7 cut(s) 82, 97, 110, 369, 613, 842, 1103
StyD4I CCNGG 4 cut(s) 344, 464, 780, 1159
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 5 cut(s) 703, 891, 940, 953, 1045
TaiI ACGT 8 cut(s) 314, 548, 630, 653, 1080, 1146, 1239, 1350
TaqII GACCGA 1 cut(s) 1078
TasI AATT 7 cut(s) 82, 97, 110, 369, 613, 842, 1103
TauI GCSGC 4 cut(s) 8, 459, 672, 963
TfiI GAWTC 8 cut(s) 491, 509, 797, 804, 852, 920, 1009, 1033
Tru1I TTAA 8 cut(s) 212, 350, 743, 789, 875, 929, 977, 1281
Tru9I TTAA 8 cut(s) 212, 350, 743, 789, 875, 929, 977, 1281
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 2 cut(s) 940, 1039
Tsp45I GTSAC 2 cut(s) 940, 1039
TspDTI ATGAA 5 cut(s) 1214, 1319, 1359, 1374, 1380
TspGWI ACGGA 4 cut(s) 380, 893, 1071, 1279
TspRI CASTG 1 cut(s) 826
Tth111I GACNNNGTC 1 cut(s) 951
VpaK11BI GGWCC 1 cut(s) 1061
VspI ATTAAT 1 cut(s) 212
XapI RAATTY 2 cut(s) 110, 842
XhoI CTCGAG 2 cut(s) 179, 479
XmiI GTMKAC 1 cut(s) 1183
XmnI GAANNNNTTC 2 cut(s) 261, 569
ZraI GACGTC 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.