Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0009430

Physical Location & Seq

Genomic Coordinates Forward (+)

18723110 .. 18727055

Gene Structure 3946 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00179120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCGTCATCCTCTCGGTGGTCCGACGGTGGGACGGAGCGGCGATGGTGGAGCTCGACATCGACGGTGGAGTGGTGGGCGTGGTGGCTCGAGAGAGAGAGAGAGAAAGAGAGCCCGAGACGAGTTGACACCAATGCCAAGGTTGAGAGAGTTGCAGTGGTGGAAGTCAAGCTCCACCACAGCGCTTTCTCAACCCTAAAGTCTCATGCCTTTGGATCAAACCTCACTCTCCCCATCACCACCACCACCACCACCACTCCATGCAAGGTGGCCTCGAAATCCAGCGAATCCCTAACCTCCACAATCACCGAGCTCGAAAGAGAACGAGCCACAGACAGAGACGAAATCTATCTCGAAGACGATATTGAGGACGACGAAGCTATTACTCATGTCAACAGTCACAACGCGGAGCGTCGTTTATCCGACGTGTGGCGGGAGCTCCACGGCGAGGACGACTGGGTCGGGTTGCTCGACCCGATGGACCCGCTCCTCCGCTCCGAGCTCATCCGCTACGGCGAGATGGCCCAGGCCTGCTACGACGCCTTCGACTTCGACCCGTTTTCCAAGTACTGCGGCAGCTGCCGTTTCACCCGCAGCAACTTCTTCGACTCCCTCGGCATGACCCACCACGGCTACCACGTCTCCCGCTACCTCTTCGCCACCTCAAATATCAACTTGCCGAATTTCTTCAAGAAGTCGCGGTGGCCCAAAGTCTGGAGCAAGAACGCCAACTGGATCGGGTACGTCGCCGTTTCCGACGACGAGATGACTGAGCGTCTGGGCAGGAGGGACATCAGCATCGCGTGGCGGGGGACGGTGACCCGGTTGGAGTGGATCGTGGACTTGATGGACTTTCTAAAACCACTCTCCACGAATAAGATCCCCTGCCCCGACCCGAATGTGAAAGCCGAATCCGGGTTCCTGGATCTTTACACCGACAAGGACGAGACGTGTAGGTTTTGTACTTACTCGGCCAGGGAGCAGATTCTGACGGAGATCAAGCGGCTGGTGGAGAAGTACCCAAACGAGGAGCTGAGCATTACGATCACCGGGCACAGTCTCGGCAGCGCGTTGGCGATTTTGAGCGCGTACGATATAGCGGAAACGGGTCTGAACGTGAAGGCGGACGGCCGGGTCGTGCCCGTGTCCGTTTTCTCGTTCTCGGGTCCTCGGGTCGGGAATGTTCACTTCAGGGAGAGACTTGAGTCTTTGGGTGTGAAGGTGTTGAGGGTTGTGAACGTGCATGACGTGGTGCCTAAGAGTCCGGGCTTGTTGATTAATGAGCACGTGCCGCCGGTGATGCTTAAGTTGACGGAGAACTTGCCGTGGAGCTACTCGCACGTCGGAGTCGAGCTCGAATTAGATCATAAGAATTCTCCATTTTTGAAGCAGACTAATGATCCAGGCTGTGCTCATAACTTGGAAGCTTACTTGCATTTGCTTGACGGATACCATGGAAAAGGCCATAGATTTGTGCTGGCAAGTGGAAGAGACCCTGCACTAGTGAACAAGGCCTCTGATTTCTTGAAGGATCATTACTTGGTACCGCCTTTCTGGAGGCAAGACGAGAACAAGGGGATGGTGAGGTGCAAGGACGGACGCTGGATTCAACCTGAAAGGCCAAAGCTTGAGGATCATCCCGAAGACATTCACCACCATCTCAAGCAATTAGGTCTTGTTTCCGATGAAGAATCAGATCGGAAACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008970 GO:0009507 GO:0009536 GO:0016298 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0047714 GO:0052689

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

569

Amino Acids

65.22

Weight (kDa)

5.91

Isoelectric Point (pI)

40.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	267 - 423	1.9e-37	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06800 AT1G06800 AT2G30550 AT2G30550
fragaria_vesca	FvH4_2g22500
malus_domestica	MD05G1205500.v1.1 MD10G1192500.v1.1
prunus_persica	Prupe.8G231900_v2.0.a1
pyrus_communis	pycom10g16620
rosa_chinensis	RchiOBHm_Chr6g0289521
rosa_laevigata	RLG00000012295
rosa_multiflora	Rmu_co8503037.1_g000001 Rmu_sc0001527.1_g000050 Rmu_sc0002417.1_g000004
rosa_roxburghii	Rroxscaffold_7G00177880 Rroxscaffold_7G00179120
rosa_rugosa	Rorug06G0204200
rosa_samantha	Rh6AG315800 Rh6BG322400 Rh6CG328800 Rh6DG314600
rosa_wichuraiana	Rw6G027220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	458, 1133
Acc65I	GGTACC	1 cut(s)	1543
AccB1I	GGYRCC	2 cut(s)	1252, 1543
AccB7I	CCANNNNNTGG	1 cut(s)	714
AccBSI	CCGCTC	3 cut(s)	40, 487, 495
AccII	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
AclWI	GGATC	8 cut(s)	223, 743, 842, 874, 933, 1394, 1539, 1641
AcoI	YGGCCR	2 cut(s)	972, 1129
AcsI	RAATTY	2 cut(s)	682, 1372
AcuI	CTGAAG	1 cut(s)	1174
AcvI	CACGTG	1 cut(s)	1288
AcyI	GRCGYC	2 cut(s)	5, 540
AfaI	GTAC	6 cut(s)	569, 743, 964, 1019, 1091, 1545
AfeI	AGCGCT	1 cut(s)	184
AfiI	CCNNNNNNNGG	8 cut(s)	18, 30, 429, 448, 714, 915, 1027, 1175
AflII	CTTAAG	1 cut(s)	1304
AflIII	ACRYGT	2 cut(s)	426, 950
AgsI	TTSAA	4 cut(s)	691, 1387, 1528, 1610
AhdI	GACNNNNNGTC	1 cut(s)	774
AhlI	ACTAGT	1 cut(s)	1501
AjiI	CACGTC	5 cut(s)	427, 640, 951, 1249, 1342
AjnI	CCWGG	4 cut(s)	525, 921, 974, 1402
Alw21I	GWGCWC	7 cut(s)	56, 315, 441, 504, 1287, 1356, 1414
Alw26I	GTCTC	8 cut(s)	112, 207, 333, 646, 941, 1064, 1192, 1485
AlwI	GGATC	8 cut(s)	223, 743, 842, 874, 933, 1394, 1539, 1641
AlwNI	CAGNNNCTG	1 cut(s)	988
Ama87I	CYCGRG	4 cut(s)	89, 115, 1162, 1170
Aor51HI	AGCGCT	1 cut(s)	184
AoxI	GGCC	9 cut(s)	270, 522, 528, 704, 972, 1129, 1462, 1512, 1619
ApeKI	GCWGC	4 cut(s)	576, 579, 594, 1065
ApoI	RAATTY	2 cut(s)	682, 1372
AseI	ATTAAT	1 cut(s)	1278
Asp700I	GAANNNNTTC	2 cut(s)	686, 1647
Asp718I	GGTACC	1 cut(s)	1543
AspLEI	GCGC	3 cut(s)	185, 1070, 1088
AspS9I	GGNCC	5 cut(s)	21, 481, 523, 705, 1166
AsuC2I	CCSGG	5 cut(s)	823, 916, 1051, 1133, 1266
AsuHPI	GGTGA	8 cut(s)	229, 298, 580, 829, 1039, 1309, 1594, 1643
AvaI	CYCGRG	4 cut(s)	89, 115, 1162, 1170
AvaII	GGWCC	3 cut(s)	21, 481, 1166
BaeGI	GKGCMC	2 cut(s)	1056, 1143
BanI	GGYRCC	2 cut(s)	1252, 1543
BanII	GRGCYC	6 cut(s)	56, 116, 315, 441, 504, 1356
BarI	GAAGNNNNNNTAC	2 cut(s)	1520, 1552
BbrPI	CACGTG	1 cut(s)	1288
BbsI	GAAGAC	2 cut(s)	363, 1650
Bbv12I	GWGCWC	7 cut(s)	56, 315, 441, 504, 1287, 1356, 1414
BbvI	GCAGC	4 cut(s)	566, 588, 606, 1077
BccI	CCATC	7 cut(s)	39, 242, 472, 514, 841, 1573, 1665
BceAI	ACGGC	7 cut(s)	460, 529, 567, 646, 734, 1144, 1309
BcgI	CGANNNNNNTGC	2 cut(s)	586, 620
BciT130I	CCWGG	4 cut(s)	527, 923, 976, 1404
BciVI	GTATCC	1 cut(s)	1442
BcnI	CCSGG	5 cut(s)	823, 916, 1051, 1133, 1266
BcoDI	GTCTC	8 cut(s)	112, 207, 333, 646, 941, 1064, 1192, 1485
BcuI	ACTAGT	1 cut(s)	1501
BfaI	CTAG	1 cut(s)	1502
BfoI	RGCGCY	1 cut(s)	186
BfrI	CTTAAG	1 cut(s)	1304
BfuI	GTATCC	1 cut(s)	1442
BisI	GCNGC	8 cut(s)	41, 574, 577, 580, 595, 1004, 1066, 1292
BlpI	GCTNAGC	1 cut(s)	1034
BlsI	GCNGC	8 cut(s)	42, 575, 578, 581, 596, 1005, 1067, 1293
BmcAI	AGTACT	1 cut(s)	569
Bme1390I	CCNGG	9 cut(s)	527, 823, 916, 923, 976, 1051, 1133, 1266, 1404
Bme18I	GGWCC	3 cut(s)	21, 481, 1166
BmeRI	GACNNNNNGTC	1 cut(s)	774
BmeT110I	CYCGRG	4 cut(s)	89, 115, 1162, 1170
BmgBI	CACGTC	5 cut(s)	427, 640, 951, 1249, 1342
BmgT120I	GGNCC	5 cut(s)	21, 481, 523, 705, 1166
BmiI	GGNNCC	5 cut(s)	483, 920, 1167, 1254, 1545
BmrFI	CCNGG	9 cut(s)	527, 823, 916, 923, 976, 1051, 1133, 1266, 1404
BmrI	ACTGGG	1 cut(s)	466
BmsI	GCATC	2 cut(s)	807, 1290
BmuI	ACTGGG	1 cut(s)	466
BoxI	GACNNNNGTC	1 cut(s)	1204
BpiI	GAAGAC	2 cut(s)	363, 1650
BplI	GAGNNNNNCTC	2 cut(s)	1338, 1370
BpmI	CTGGAG	2 cut(s)	736, 1576
Bpu1102I	GCTNAGC	1 cut(s)	1034
BpuEI	CTTGAG	3 cut(s)	1223, 1646, 1649
BpuMI	CCSGG	5 cut(s)	823, 916, 1051, 1133, 1266
BsaAI	YACGTR	1 cut(s)	1288
BsaHI	GRCGYC	2 cut(s)	5, 540
BsaI	GGTCTC	1 cut(s)	1485
BsaJI	CCNNGG	9 cut(s)	138, 442, 525, 613, 628, 975, 1169, 1325, 1453
BsaXI	ACNNNNNCTCC	4 cut(s)	241, 271, 626, 656
Bsc4I	CCNNNNNNNGG	8 cut(s)	18, 30, 429, 448, 714, 915, 1027, 1175
Bse118I	RCCGGY	1 cut(s)	1294
Bse1I	ACTGG	2 cut(s)	461, 737
BseBI	CCWGG	4 cut(s)	527, 923, 976, 1404
BseDI	CCNNGG	9 cut(s)	138, 442, 525, 613, 628, 975, 1169, 1325, 1453
BseGI	GGATG	4 cut(s)	8, 504, 1584, 1636
BseLI	CCNNNNNNNGG	8 cut(s)	18, 30, 429, 448, 714, 915, 1027, 1175
BseMII	CTCAG	2 cut(s)	762, 1025
BseNI	ACTGG	2 cut(s)	461, 737
BseRI	GAGGAG	2 cut(s)	479, 1043
BseSI	GKGCMC	2 cut(s)	1056, 1143
BseX3I	CGGCCG	1 cut(s)	1129
BseXI	GCAGC	4 cut(s)	566, 588, 606, 1077
BsgI	GTGCAG	1 cut(s)	1482
Bsh1236I	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
Bsh1285I	CGRYCG	1 cut(s)	1132
BshFI	GGCC	9 cut(s)	272, 524, 530, 706, 974, 1131, 1464, 1514, 1621
BshNI	GGYRCC	2 cut(s)	1252, 1543
BsiEI	CGRYCG	1 cut(s)	1132
BsiHKAI	GWGCWC	7 cut(s)	56, 315, 441, 504, 1287, 1356, 1414
BsiHKCI	CYCGRG	4 cut(s)	89, 115, 1162, 1170
BsiSI	CCGG	6 cut(s)	823, 915, 1050, 1132, 1265, 1295
BsiWI	CGTACG	1 cut(s)	1089
BslFI	GGGAC	3 cut(s)	46, 803, 826
BslI	CCNNNNNNNGG	8 cut(s)	18, 30, 429, 448, 714, 915, 1027, 1175
BsmAI	GTCTC	8 cut(s)	112, 207, 333, 646, 941, 1064, 1192, 1485
BsmBI	CGTCTC	4 cut(s)	112, 333, 646, 941
BsmFI	GGGAC	3 cut(s)	46, 803, 826
BsnI	GGCC	9 cut(s)	272, 524, 530, 706, 974, 1131, 1464, 1514, 1621
Bso31I	GGTCTC	1 cut(s)	1485
BsoBI	CYCGRG	4 cut(s)	89, 115, 1162, 1170
Bsp1720I	GCTNAGC	1 cut(s)	1034
Bsp19I	CCATGG	1 cut(s)	1453
BspANI	GGCC	9 cut(s)	272, 524, 530, 706, 974, 1131, 1464, 1514, 1621
BspCNI	CTCAG	2 cut(s)	763, 1026
BspFNI	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
BspLI	GGNNCC	5 cut(s)	483, 920, 1167, 1254, 1545
BspPI	GGATC	8 cut(s)	223, 743, 842, 874, 933, 1394, 1539, 1641
BspT107I	GGYRCC	2 cut(s)	1252, 1543
BspTI	CTTAAG	1 cut(s)	1304
BspTNI	GGTCTC	1 cut(s)	1485
BsrBI	CCGCTC	3 cut(s)	40, 487, 495
BsrFI	RCCGGY	1 cut(s)	1294
BsrI	ACTGG	2 cut(s)	461, 737
BssAI	RCCGGY	1 cut(s)	1294
BssECI	CCNNGG	9 cut(s)	138, 442, 525, 613, 628, 975, 1169, 1325, 1453
BssNI	GRCGYC	2 cut(s)	5, 540
BssT1I	CCWWGG	2 cut(s)	138, 1453
Bst2UI	CCWGG	4 cut(s)	527, 923, 976, 1404
Bst4CI	ACNGT	6 cut(s)	29, 67, 398, 817, 1058, 1707
Bst6I	CTCTTC	2 cut(s)	659, 1483
BstACI	GRCGYC	2 cut(s)	5, 540
BstAFI	CTTAAG	1 cut(s)	1304
BstBAI	YACGTR	1 cut(s)	1288
BstC8I	GCNNGC	2 cut(s)	532, 1479
BstDEI	CTNAG	3 cut(s)	771, 1034, 1257
BstDSI	CCRYGG	4 cut(s)	442, 628, 1325, 1453
BstEII	GGTNACC	1 cut(s)	817
BstF5I	GGATG	4 cut(s)	8, 504, 1584, 1636
BstFNI	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
BstH2I	RGCGCY	1 cut(s)	186
BstHHI	GCGC	3 cut(s)	185, 1070, 1088
BstMAI	GTCTC	8 cut(s)	112, 207, 333, 646, 941, 1064, 1192, 1485
BstMCI	CGRYCG	1 cut(s)	1132
BstMWI	GCNNNNNNNGC	3 cut(s)	579, 1291, 1300
BstNI	CCWGG	4 cut(s)	527, 923, 976, 1404
BstPAI	GACNNNNGTC	1 cut(s)	1204
BstPI	GGTNACC	1 cut(s)	817
BstSCI	CCNGG	9 cut(s)	525, 821, 914, 921, 974, 1049, 1131, 1264, 1402
BstSLI	GKGCMC	2 cut(s)	1056, 1143
BstUI	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
BstV1I	GCAGC	4 cut(s)	566, 588, 606, 1077
BstV2I	GAAGAC	2 cut(s)	363, 1650
BstX2I	RGATCY	2 cut(s)	879, 925
BstYI	RGATCY	2 cut(s)	879, 925
BstZI	CGGCCG	1 cut(s)	1129
BsuI	GTATCC	1 cut(s)	1442
BsuRI	GGCC	9 cut(s)	272, 524, 530, 706, 974, 1131, 1464, 1514, 1621
BtgI	CCRYGG	4 cut(s)	442, 628, 1325, 1453
BtgZI	GCGATG	2 cut(s)	58, 784
BtrI	CACGTC	5 cut(s)	427, 640, 951, 1249, 1342
BtsCI	GGATG	4 cut(s)	8, 504, 1584, 1636
BtsI	GCAGTG	1 cut(s)	162
BtsIMutI	CAGTG	1 cut(s)	162
Cac8I	GCNNGC	2 cut(s)	532, 1479
CaiI	CAGNNNCTG	1 cut(s)	988
CfoI	GCGC	3 cut(s)	185, 1070, 1088
Cfr10I	RCCGGY	1 cut(s)	1294
Cfr13I	GGNCC	5 cut(s)	21, 481, 523, 705, 1166
CseI	GACGC	4 cut(s)	401, 548, 764, 1608
Csp6I	GTAC	6 cut(s)	568, 742, 963, 1018, 1090, 1544
CviAII	CATG	6 cut(s)	206, 261, 389, 619, 1244, 1454
CviQI	GTAC	6 cut(s)	568, 742, 963, 1018, 1090, 1544
DdeI	CTNAG	3 cut(s)	771, 1034, 1257
DrdI	GACNNNNNNGTC	2 cut(s)	458, 1133
DriI	GACNNNNNGTC	1 cut(s)	774
DseDI	GACNNNNNNGTC	2 cut(s)	458, 1133
EaeI	YGGCCR	2 cut(s)	972, 1129
EagI	CGGCCG	1 cut(s)	1129
Eam1104I	CTCTTC	2 cut(s)	659, 1483
Eam1105I	GACNNNNNGTC	1 cut(s)	774
EarI	CTCTTC	2 cut(s)	659, 1483
EciI	GGCGGA	1 cut(s)	1139
Ecl136II	GAGCTC	5 cut(s)	54, 313, 439, 502, 1354
EclXI	CGGCCG	1 cut(s)	1129
Eco130I	CCWWGG	2 cut(s)	138, 1453
Eco147I	AGGCCT	2 cut(s)	530, 1514
Eco24I	GRGCYC	6 cut(s)	56, 116, 315, 441, 504, 1356
Eco31I	GGTCTC	1 cut(s)	1485
Eco47I	GGWCC	3 cut(s)	21, 481, 1166
Eco47III	AGCGCT	1 cut(s)	184
Eco52I	CGGCCG	1 cut(s)	1129
Eco53kI	GAGCTC	5 cut(s)	54, 313, 439, 502, 1354
Eco57I	CTGAAG	1 cut(s)	1174
Eco72I	CACGTG	1 cut(s)	1288
Eco88I	CYCGRG	4 cut(s)	89, 115, 1162, 1170
Eco91I	GGTNACC	1 cut(s)	817
EcoICRI	GAGCTC	5 cut(s)	54, 313, 439, 502, 1354
EcoO109I	RGGNCCY	1 cut(s)	1166
EcoO65I	GGTNACC	1 cut(s)	817
EcoRI	GAATTC	1 cut(s)	1372
EcoRII	CCWGG	4 cut(s)	525, 921, 974, 1402
EcoT14I	CCWWGG	2 cut(s)	138, 1453
EcoT38I	GRGCYC	6 cut(s)	56, 116, 315, 441, 504, 1356
ErhI	CCWWGG	2 cut(s)	138, 1453
Esp3I	CGTCTC	4 cut(s)	112, 333, 646, 941
FaeI	CATG	6 cut(s)	209, 264, 392, 622, 1247, 1457
FaqI	GGGAC	3 cut(s)	46, 803, 826
FatI	CATG	6 cut(s)	205, 260, 388, 618, 1243, 1453
FauI	CCCGC	5 cut(s)	426, 492, 599, 653, 801
Fnu4HI	GCNGC	8 cut(s)	41, 574, 577, 580, 595, 1004, 1066, 1292
FokI	GGATG	3 cut(s)	491, 1591, 1623
FriOI	GRGCYC	6 cut(s)	56, 116, 315, 441, 504, 1356
Fsp4HI	GCNGC	8 cut(s)	41, 574, 577, 580, 595, 1004, 1066, 1292
FspBI	CTAG	1 cut(s)	1502
GlaI	GCGC	3 cut(s)	184, 1069, 1087
GluI	GCNGC	8 cut(s)	41, 574, 577, 580, 595, 1004, 1066, 1292
GsuI	CTGGAG	2 cut(s)	736, 1576
HaeII	RGCGCY	1 cut(s)	186
HaeIII	GGCC	9 cut(s)	272, 524, 530, 706, 974, 1131, 1464, 1514, 1621
HapII	CCGG	6 cut(s)	823, 915, 1050, 1132, 1265, 1295
HgaI	GACGC	4 cut(s)	401, 548, 764, 1608
HhaI	GCGC	3 cut(s)	185, 1070, 1088
Hin1I	GRCGYC	2 cut(s)	5, 540
Hin1II	CATG	6 cut(s)	209, 264, 392, 622, 1247, 1457
Hin6I	GCGC	3 cut(s)	183, 1068, 1086
HinP1I	GCGC	3 cut(s)	183, 1068, 1086
HincII	GTYRAC	3 cut(s)	127, 394, 1311
HindII	GTYRAC	3 cut(s)	127, 394, 1311
HindIII	AAGCTT	2 cut(s)	1425, 1625
HinfI	GANTC	9 cut(s)	287, 608, 911, 985, 1205, 1261, 1347, 1606, 1691
HpaII	CCGG	6 cut(s)	823, 915, 1050, 1132, 1265, 1295
HphI	GGTGA	8 cut(s)	229, 298, 580, 829, 1039, 1309, 1594, 1643
Hpy166II	GTNNAC	7 cut(s)	127, 394, 841, 1186, 1237, 1311, 1507
Hpy188III	TCNNGA	8 cut(s)	91, 353, 691, 715, 1177, 1525, 1555, 1640
Hpy8I	GTNNAC	7 cut(s)	127, 394, 841, 1186, 1237, 1311, 1507
HpyAV	CCTTC	4 cut(s)	553, 1114, 1213, 1522
HpyCH4III	ACNGT	6 cut(s)	29, 67, 398, 817, 1058, 1707
HpyCH4IV	ACGT	9 cut(s)	426, 639, 744, 950, 1116, 1239, 1248, 1287, 1341
HpyCH4V	TGCA	6 cut(s)	155, 264, 1243, 1435, 1499, 1590
HpyF10VI	GCNNNNNNNGC	3 cut(s)	579, 1291, 1300
HpyF3I	CTNAG	3 cut(s)	771, 1034, 1257
HpySE526I	ACGT	9 cut(s)	426, 639, 744, 950, 1116, 1239, 1248, 1287, 1341
Hsp92I	GRCGYC	2 cut(s)	5, 540
Hsp92II	CATG	6 cut(s)	209, 264, 392, 622, 1247, 1457
HspAI	GCGC	3 cut(s)	183, 1068, 1086
KpnI	GGTACC	1 cut(s)	1547
Lsp1109I	GCAGC	4 cut(s)	566, 588, 606, 1077
LweI	GCATC	2 cut(s)	807, 1290
MaeI	CTAG	1 cut(s)	1502
MaeII	ACGT	9 cut(s)	426, 639, 744, 950, 1116, 1239, 1248, 1287, 1341
MaeIII	GTNAC	2 cut(s)	398, 817
MbiI	CCGCTC	3 cut(s)	40, 487, 495
MboII	GAAGA	7 cut(s)	368, 595, 646, 679, 1500, 1655, 1700
MflI	RGATCY	2 cut(s)	879, 925
MluCI	AATT	4 cut(s)	682, 1358, 1372, 1667
MlyI	GAGTC	4 cut(s)	602, 1214, 1270, 1356
MmeI	TCCRAC	5 cut(s)	48, 447, 780, 807, 1324
MroXI	GAANNNNTTC	2 cut(s)	686, 1647
MseI	TTAA	2 cut(s)	1278, 1305
MspA1I	CMGCKG	1 cut(s)	579
MspCI	CTTAAG	1 cut(s)	1304
MspI	CCGG	6 cut(s)	823, 915, 1050, 1132, 1265, 1295
MspR9I	CCNGG	9 cut(s)	527, 823, 916, 923, 976, 1051, 1133, 1266, 1404
MvaI	CCWGG	4 cut(s)	527, 923, 976, 1404
MvnI	CGCG	5 cut(s)	407, 700, 803, 1070, 1088
MwoI	GCNNNNNNNGC	3 cut(s)	579, 1291, 1300
NciI	CCSGG	5 cut(s)	823, 916, 1051, 1133, 1266
NcoI	CCATGG	1 cut(s)	1453
NlaIII	CATG	6 cut(s)	209, 264, 392, 622, 1247, 1457
NlaIV	GGNNCC	5 cut(s)	483, 920, 1167, 1254, 1545
NmeAIII	GCCGAG	3 cut(s)	594, 950, 1041
NmuCI	GTSAC	2 cut(s)	398, 817
PaeR7I	CTCGAG	1 cut(s)	89
PceI	AGGCCT	2 cut(s)	530, 1514
PcsI	WCGNNNNNNNCGW	8 cut(s)	450, 468, 543, 636, 753, 1134, 1245, 1347
PdmI	GAANNNNTTC	2 cut(s)	686, 1647
PfeI	GAWTC	5 cut(s)	287, 911, 985, 1606, 1691
Pfl23II	CGTACG	1 cut(s)	1089
PflFI	GACNNNGTC	1 cut(s)	458
PflMI	CCANNNNNTGG	1 cut(s)	714
PfoI	TCCNGGA	1 cut(s)	921
PkrI	GCNGC	8 cut(s)	42, 575, 578, 581, 596, 1005, 1067, 1293
PleI	GAGTC	4 cut(s)	602, 1213, 1269, 1355
PmaCI	CACGTG	1 cut(s)	1288
PmlI	CACGTG	1 cut(s)	1288
PpsI	GAGTC	4 cut(s)	602, 1213, 1269, 1355
Ppu21I	YACGTR	1 cut(s)	1288
PpuMI	RGGWCCY	1 cut(s)	1166
PshAI	GACNNNNGTC	1 cut(s)	1204
PshBI	ATTAAT	1 cut(s)	1278
Psp124BI	GAGCTC	5 cut(s)	56, 315, 441, 504, 1356
Psp5II	RGGWCCY	1 cut(s)	1166
Psp6I	CCWGG	4 cut(s)	525, 921, 974, 1402
PspCI	CACGTG	1 cut(s)	1288
PspEI	GGTNACC	1 cut(s)	817
PspGI	CCWGG	4 cut(s)	525, 921, 974, 1402
PspLI	CGTACG	1 cut(s)	1089
PspN4I	GGNNCC	5 cut(s)	483, 920, 1167, 1254, 1545
PspPI	GGNCC	5 cut(s)	21, 481, 523, 705, 1166
PspPPI	RGGWCCY	1 cut(s)	1166
PstNI	CAGNNNCTG	1 cut(s)	988
PsuI	RGATCY	2 cut(s)	879, 925
PsyI	GACNNNGTC	1 cut(s)	458
PvuII	CAGCTG	1 cut(s)	579
RsaI	GTAC	6 cut(s)	569, 743, 964, 1019, 1091, 1545
RsaNI	GTAC	6 cut(s)	568, 742, 963, 1018, 1090, 1544
SacI	GAGCTC	5 cut(s)	56, 315, 441, 504, 1356
SaqAI	TTAA	2 cut(s)	1278, 1305
SatI	GCNGC	8 cut(s)	41, 574, 577, 580, 595, 1004, 1066, 1292
Sau96I	GGNCC	5 cut(s)	21, 481, 523, 705, 1166
ScaI	AGTACT	1 cut(s)	569
SchI	GAGTC	4 cut(s)	602, 1214, 1270, 1356
ScrFI	CCNGG	9 cut(s)	527, 823, 916, 923, 976, 1051, 1133, 1266, 1404
SfaNI	GCATC	2 cut(s)	807, 1290
Sfr274I	CTCGAG	1 cut(s)	89
SgrAI	CRCCGGYG	1 cut(s)	1294
SinI	GGWCC	3 cut(s)	21, 481, 1166
SlaI	CTCGAG	1 cut(s)	89
SmlI	CTYRAG	5 cut(s)	89, 1202, 1304, 1628, 1661
SmoI	CTYRAG	5 cut(s)	89, 1202, 1304, 1628, 1661
SpeI	ACTAGT	1 cut(s)	1501
Sse9I	AATT	4 cut(s)	682, 1358, 1372, 1667
SseBI	AGGCCT	2 cut(s)	530, 1514
SspMI	CTAG	1 cut(s)	1502
SstI	GAGCTC	5 cut(s)	56, 315, 441, 504, 1356
StuI	AGGCCT	2 cut(s)	530, 1514
StyD4I	CCNGG	9 cut(s)	525, 821, 914, 921, 974, 1049, 1131, 1264, 1402
StyI	CCWWGG	2 cut(s)	138, 1453
TaaI	ACNGT	6 cut(s)	29, 67, 398, 817, 1058, 1707
TaiI	ACGT	9 cut(s)	429, 642, 747, 953, 1119, 1242, 1251, 1290, 1344
TasI	AATT	4 cut(s)	682, 1358, 1372, 1667
TatI	WGTACW	2 cut(s)	567, 962
TauI	GCSGC	4 cut(s)	43, 576, 1006, 1294
TfiI	GAWTC	5 cut(s)	287, 911, 985, 1606, 1691
Tru1I	TTAA	2 cut(s)	1278, 1305
Tru9I	TTAA	2 cut(s)	1278, 1305
TscAI	CASTG	1 cut(s)	162
TseFI	GTSAC	2 cut(s)	398, 817
TseI	GCWGC	4 cut(s)	576, 579, 594, 1065
Tsp45I	GTSAC	2 cut(s)	398, 817
TspDTI	ATGAA	1 cut(s)	1701
TspGWI	ACGGA	6 cut(s)	50, 1007, 1138, 1328, 1461, 1611
TspRI	CASTG	1 cut(s)	162
Tth111I	GACNNNGTC	1 cut(s)	458
Van91I	CCANNNNNTGG	1 cut(s)	714
Vha464I	CTTAAG	1 cut(s)	1304
VpaK11BI	GGWCC	3 cut(s)	21, 481, 1166
VspI	ATTAAT	1 cut(s)	1278
XapI	RAATTY	2 cut(s)	682, 1372
XhoI	CTCGAG	1 cut(s)	89
XmnI	GAANNNNTTC	2 cut(s)	686, 1647
XspI	CTAG	1 cut(s)	1502
ZrmI	AGTACT	1 cut(s)	569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.