AT2G45920

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0018313
Physical Location & Seq
Forward (+)
18898629 .. 18901326
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45920.1

Sequence Viewer

Length: 1203 bp
ATGATGGGCAAATCAGGTTCGCAGCAGCTGCTGATGGATGAGAAGATATATGTTGCAGTGGGGAGTGACTTGGGGAACAAGTCCACTCTTGTTTGGGCGATACAGAACACTGGAGGCAAGGAGTTCTGTATCGTCCACGTTCACCAGCCACTCTACAGAAAAGAAAAGGAAAAGACACAAAAGATCCTGGACAAATACCTACAAAAATGCAGGCAAATGCAGGTCTGTGCTGAGATGATACACATTAAAATGGAGTCGGTAGAGAAAGGGATCATCCAATTGATCTCAGAGCGCAATGTCAAGAAGCTCGTCATGGGAGCTGCGTCAGACACACGCTATTCAATGAGGATGGCAGATCTGTTGTCCACGAAAGCCATTTACATTCGTCAAGAAGCGCCTGCTACTTGTTGTATATGGTTTACTTGCAAAGGATACCTGGTATATAAAAGGGAATCTATCATGGGCAACACCTCTCTAGAGTATGCATCTACAAGTTCTGGTCAAGATTCAGTCCGTTCACGAGGATCTGTTATACCTTCGCGCCAATTCACGATATCTAGAGGAAATGGAAATGTGTATCAGTTAGCTGTCTTTGAAGCTGAGAAATCGAAAAAAGAAGCAAGCTTGGAGGCTTTCAAGCATCAAGAAGTTGTAAAAGAGAAAAATGAAGCAATCAAAAGGGGTAAAGAATGGGAAAGTGCCTATTTGGAGGAGTTGAAACAAAGGAAAGAAACAGAGATGGAGCTGAAGAAAGTGAGAGAAAAACTCGAAAAGATGAGGTATATATCTGAGAACCGAATAACCGAGTCTTATATGTTGGTACAAAAGCTGCAAGACAAGTATAACTTGGCAACGAAAGTGTTGAGAAAAGCCAAGGAAGAACGAGATTTATTGATTAAAGGACGCGACATAGCAATTATAGAAGTAGAGGAGCTAAGAAAAGAGGTCTCTCGATCTGATGAGCACCGTGAAGCTCCTCAGTACTTCATCTGTCCCATTTCACTGGAAGTGATGAAGGATCCACAACTAGCAGCAGATGGATTTACATATGAAGCAGAAGCCATAAGTACATGGCTTCAGGGAGGACATGAGACTTCCCCAATGACAAACACGAAGCTTCATCATACAAAGTTAGTTCCAAATCTTGCTCTTCGATCTGCAATCCAGGAATGGCTTCACGCTTCATCCTCTTTTAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

400

Amino Acids

46.02

Weight (kDa)

9.14

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 325 - 394 8.5e-20 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45920 AT2G45920 AT3G61390 AT3G61390 AT3G61390 AT3G61390 AT3G61390 AT3G61410 AT3G61410 AT3G61410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 211
AccII CGCG 2 cut(s) 541, 906
AclWI GGATC 5 cut(s) 178, 278, 532, 1013, 1026
AcuI CTGAAG 2 cut(s) 767, 1061
AfaI GTAC 3 cut(s) 822, 983, 1069
AgsI TTSAA 4 cut(s) 342, 596, 637, 718
AjnI CCWGG 3 cut(s) 186, 435, 1164
Alw21I GWGCWC 1 cut(s) 966
Alw26I GTCTC 2 cut(s) 952, 1085
AlwI GGATC 5 cut(s) 178, 278, 532, 1013, 1026
AlwNI CAGNNNCTG 2 cut(s) 28, 31
ApeKI GCWGC 6 cut(s) 22, 25, 28, 320, 829, 1031
Asp700I GAANNNNTTC 1 cut(s) 1173
AspLEI GCGC 3 cut(s) 294, 397, 543
AsuHPI GGTGA 1 cut(s) 134
BamHI GGATCC 1 cut(s) 1018
BauI CACGAG 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 966
BbvI GCAGC 6 cut(s) 15, 34, 37, 307, 816, 1043
BccI CCATC 4 cut(s) 28, 343, 733, 1031
BciT130I CCWGG 3 cut(s) 188, 437, 1166
BciVI GTATCC 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 952, 1085
BfaI CTAG 3 cut(s) 476, 558, 1028
BfmI CTRYAG 1 cut(s) 154
BfoI RGCGCY 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 211
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 1 cut(s) 355
BisI GCNGC 6 cut(s) 23, 26, 29, 321, 830, 1032
BlsI GCNGC 6 cut(s) 24, 27, 30, 322, 831, 1033
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 3 cut(s) 188, 437, 1166
BmiI GGNNCC 1 cut(s) 1020
BmrFI CCNGG 3 cut(s) 188, 437, 1166
BmsI GCATC 2 cut(s) 494, 649
BplI GAGNNNNNCTC 2 cut(s) 750, 782
BpmI CTGGAG 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 952
BsaJI CCNNGG 1 cut(s) 873
Bse1I ACTGG 2 cut(s) 115, 1008
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 3 cut(s) 188, 437, 1166
BseDI CCNNGG 1 cut(s) 873
BseGI GGATG 4 cut(s) 43, 273, 354, 1184
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 5 cut(s) 222, 300, 591, 780, 992
BseNI ACTGG 2 cut(s) 115, 1008
BseRI GAGGAG 3 cut(s) 725, 944, 966
BseXI GCAGC 6 cut(s) 15, 34, 37, 307, 816, 1043
Bsh1236I CGCG 2 cut(s) 541, 906
BsiHKAI GWGCWC 1 cut(s) 966
BslFI GGGAC 1 cut(s) 978
BsmAI GTCTC 2 cut(s) 952, 1085
BsmFI GGGAC 1 cut(s) 978
Bso31I GGTCTC 1 cut(s) 952
Bsp1286I GDGCHC 1 cut(s) 966
Bsp143I GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
BspCNI CTCAG 5 cut(s) 223, 299, 592, 781, 991
BspFNI CGCG 2 cut(s) 541, 906
BspLI GGNNCC 1 cut(s) 1020
BspMI ACCTGC 1 cut(s) 211
BspPI GGATC 5 cut(s) 178, 278, 532, 1013, 1026
BspQI GCTCTTC 1 cut(s) 1155
BspTNI GGTCTC 1 cut(s) 952
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 2 cut(s) 115, 1008
BssECI CCNNGG 1 cut(s) 873
BssMI GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 873
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 3 cut(s) 188, 437, 1166
Bst4CI ACNGT 1 cut(s) 968
Bst6I CTCTTC 1 cut(s) 1155
BstAPI GCANNNNNTGC 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 212, 399, 622
BstDEI CTNAG 6 cut(s) 231, 286, 600, 789, 935, 978
BstF5I GGATG 4 cut(s) 43, 273, 354, 1184
BstFNI CGCG 2 cut(s) 541, 906
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 3 cut(s) 294, 397, 543
BstKTI GATC 8 cut(s) 186, 273, 285, 358, 527, 956, 1021, 1157
BstMAI GTCTC 2 cut(s) 952, 1085
BstMBI GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNI CCWGG 3 cut(s) 188, 437, 1166
BstSCI CCNGG 3 cut(s) 186, 435, 1164
BstSFI CTRYAG 1 cut(s) 154
BstUI CGCG 2 cut(s) 541, 906
BstV1I GCAGC 6 cut(s) 15, 34, 37, 307, 816, 1043
BstX2I RGATCY 4 cut(s) 183, 355, 524, 1018
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 4 cut(s) 183, 355, 524, 1018
BsuI GTATCC 1 cut(s) 425
BtsCI GGATG 4 cut(s) 43, 273, 354, 1184
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 108, 1001
BveI ACCTGC 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 212, 399, 622
CaiI CAGNNNCTG 2 cut(s) 28, 31
CfoI GCGC 3 cut(s) 294, 397, 543
CseI GACGC 2 cut(s) 312, 912
CsiI ACCWGGT 1 cut(s) 435
Csp6I GTAC 3 cut(s) 821, 982, 1068
CviAII CATG 4 cut(s) 313, 460, 1071, 1088
CviQI GTAC 3 cut(s) 821, 982, 1068
DdeI CTNAG 6 cut(s) 231, 286, 600, 789, 935, 978
DpnI GATC 8 cut(s) 185, 272, 284, 357, 526, 955, 1020, 1156
DpnII GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
Eam1104I CTCTTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 1155
Eco130I CCWWGG 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 952
Eco32I GATATC 1 cut(s) 555
Eco57I CTGAAG 2 cut(s) 767, 1061
EcoRII CCWGG 3 cut(s) 186, 435, 1164
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 873
EcoT22I ATGCAT 1 cut(s) 487
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 4 cut(s) 316, 463, 1074, 1091
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FaqI GGGAC 1 cut(s) 978
FatI CATG 4 cut(s) 312, 459, 1070, 1087
FauNDI CATATG 1 cut(s) 1048
Fnu4HI GCNGC 6 cut(s) 23, 26, 29, 321, 830, 1032
FokI GGATG 4 cut(s) 50, 260, 361, 1171
Fsp4HI GCNGC 6 cut(s) 23, 26, 29, 321, 830, 1032
FspBI CTAG 3 cut(s) 476, 558, 1028
GlaI GCGC 3 cut(s) 293, 396, 542
GluI GCNGC 6 cut(s) 23, 26, 29, 321, 830, 1032
GsuI CTGGAG 1 cut(s) 132
HaeII RGCGCY 1 cut(s) 398
HgaI GACGC 2 cut(s) 312, 912
HhaI GCGC 3 cut(s) 294, 397, 543
Hin1II CATG 4 cut(s) 316, 463, 1074, 1091
Hin6I GCGC 3 cut(s) 292, 395, 541
HinP1I GCGC 3 cut(s) 292, 395, 541
HindIII AAGCTT 2 cut(s) 622, 1115
HinfI GANTC 4 cut(s) 254, 452, 506, 806
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 6 cut(s) 84, 136, 142, 366, 420, 518
Hpy188I TCNGA 4 cut(s) 289, 328, 790, 958
Hpy188III TCNNGA 9 cut(s) 301, 389, 476, 503, 519, 550, 558, 644, 951
Hpy8I GTNNAC 6 cut(s) 84, 136, 142, 366, 420, 518
HpyAV CCTTC 2 cut(s) 546, 1009
HpyCH4III ACNGT 1 cut(s) 968
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 7 cut(s) 56, 210, 220, 426, 485, 832, 1160
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 6 cut(s) 231, 286, 600, 789, 935, 978
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 4 cut(s) 316, 463, 1074, 1091
HspAI GCGC 3 cut(s) 292, 395, 541
Kzo9I GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 4 cut(s) 317, 742, 931, 979
Lsp1109I GCAGC 6 cut(s) 15, 34, 37, 307, 816, 1043
LweI GCATC 2 cut(s) 494, 649
MabI ACCWGGT 1 cut(s) 435
MaeI CTAG 3 cut(s) 476, 558, 1028
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 65
MalI GATC 8 cut(s) 185, 272, 284, 357, 526, 955, 1020, 1156
MboI GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
MboII GAAGA 4 cut(s) 55, 760, 890, 1142
MfeI CAATTG 1 cut(s) 278
MflI RGATCY 4 cut(s) 183, 355, 524, 1018
MhlI GDGCHC 1 cut(s) 966
MluCI AATT 3 cut(s) 278, 545, 915
MlyI GAGTC 2 cut(s) 263, 815
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 1173
MseI TTAA 2 cut(s) 246, 897
MslI CAYNNNNRTG 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 28
MspR9I CCNGG 3 cut(s) 188, 437, 1166
MunI CAATTG 1 cut(s) 278
MvaI CCWGG 3 cut(s) 188, 437, 1166
MvnI CGCG 2 cut(s) 541, 906
MwoI GCNNNNNNNGC 1 cut(s) 28
NdeI CATATG 1 cut(s) 1048
NdeII GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
NlaIII CATG 4 cut(s) 316, 463, 1074, 1091
NlaIV GGNNCC 1 cut(s) 1020
NmuCI GTSAC 1 cut(s) 65
NsiI ATGCAT 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1173
PfeI GAWTC 2 cut(s) 452, 506
PfoI TCCNGGA 2 cut(s) 186, 1164
PkrI GCNGC 6 cut(s) 24, 27, 30, 322, 831, 1033
PleI GAGTC 2 cut(s) 262, 814
PpsI GAGTC 2 cut(s) 262, 814
Psp6I CCWGG 3 cut(s) 186, 435, 1164
PspGI CCWGG 3 cut(s) 186, 435, 1164
PspN4I GGNNCC 1 cut(s) 1020
PstNI CAGNNNCTG 2 cut(s) 28, 31
PsuI RGATCY 4 cut(s) 183, 355, 524, 1018
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 3 cut(s) 822, 983, 1069
RsaNI GTAC 3 cut(s) 821, 982, 1068
RseI CAYNNNNRTG 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 2 cut(s) 246, 897
SatI GCNGC 6 cut(s) 23, 26, 29, 321, 830, 1032
Sau3AI GATC 8 cut(s) 183, 270, 282, 355, 524, 953, 1018, 1154
ScaI AGTACT 1 cut(s) 983
SchI GAGTC 2 cut(s) 263, 815
ScrFI CCNGG 3 cut(s) 188, 437, 1166
SduI GDGCHC 1 cut(s) 966
SexAI ACCWGGT 1 cut(s) 435
SfaNI GCATC 2 cut(s) 494, 649
SfcI CTRYAG 1 cut(s) 154
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 3 cut(s) 278, 545, 915
SspMI CTAG 3 cut(s) 476, 558, 1028
StyD4I CCNGG 3 cut(s) 186, 435, 1164
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 1 cut(s) 968
TaiI ACGT 1 cut(s) 141
TaqI TCGA 4 cut(s) 608, 768, 952, 1153
TasI AATT 3 cut(s) 278, 545, 915
TatI WGTACW 2 cut(s) 981, 1067
TfiI GAWTC 2 cut(s) 452, 506
Tru1I TTAA 2 cut(s) 246, 897
Tru9I TTAA 2 cut(s) 246, 897
TscAI CASTG 3 cut(s) 63, 115, 1008
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 6 cut(s) 22, 25, 28, 320, 829, 1031
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 6 cut(s) 681, 976, 1028, 1065, 1109, 1173
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 3 cut(s) 63, 115, 1008
XbaI TCTAGA 2 cut(s) 475, 557
XmnI GAANNNNTTC 1 cut(s) 1173
XspI CTAG 3 cut(s) 476, 558, 1028
ZrmI AGTACT 1 cut(s) 983
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.