AT3G15200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0020990
Physical Location & Seq
Reverse (-)
5117489 .. 5119060
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G15200.1

Sequence Viewer

Length: 1572 bp
ATGGGAGGGAAAAACAAAATTGAAATCTTGTTTCAGCTACAAAGTGCTCTATCGCGGCGCCGGATCTCATCATTGGTCGATTTTCGCCGTGTTTTTCCTCCCGAGACACCGAATCATAAGAACAATATCACTCTCTCCAGGTTCCACTTTCTGGGTACACACTTTCTTCATTCTCTTGGAGCTCCCGACAAATTTCCAAATAGATTCAACGACGACAAAGACAAGCAATCTGCTCTTGATGTTCATAACATCATAAAGCATCACCGTGGAAGTTCACCTGAAAAGATTAAGCGGATTCTCGATAAGTGCGGGATAGACTTAACCGAAGAGCTTGTTTTAGAAGTGGTTAATCGGAATCGATCTGATTGGAAACCAGCTTATATACTTTCTCAATTGGTCGTTAAACAATCTGTTCATCTATCTAGCTCGATGTTGTATAACGAGATTCTCGATGTTCTTGGGAAAATGCGGCGATTTGAAGAGTTTCACCAAGTGTTCGACGAAATGTCTAAGAGAGATGGATTTGTTAACGAGAAGACGTACGAAGTTTTGTTAAACCGTTACGCTGCTGCACATAAAGTTGATGAAGCTGTAGGAGTCTTTGAGAGGAGGAAAGAGTTTGGGATAGATGATGATCTGGTTGCGTTTCATGGGCTTTTGATGTGGTTGTGTAGGTATAAGCATGTTGAATTTGCGGAAACTTTGTTTTGCTCTCGTAGAAGAGAGTTTGGTTGCGATATAAAAGCGATGAATATGATTCTCAACGGGTGGTGTGTATTAGGAAATGTTCATGAAGCTAAGAGGTTTTGGAAAGATATCATTGCTTCTAAATGCAGGCCAGATGTTGTTAGCTATGGGACAATGATAAATGCATTGACGAAGAAGGGGAAATTGGGAAAGGCTATGGAATTATACAGAGCAATGTGGGACACCAGAAGAAACCCGGATGTTAAAATCTGCAATAACGTTATTGATGCTTTGTGTTTCAAGAAGAGAATCCCTGAGGCTCTTGAAGTATTCAGGGAGATTAGTGAGAAAGGTCCCGACCCGAATGTTGTGACGTATAACTCACTTTTGAAGCATCTCTGCAAGATAAGGAGAACTGAGAAAGTTTGGGAGCTAGTGGAAGAGATGGAACTTAAAGGTGGTAGTTGTTCGCCTAACGATGTGACATTCAGTTACTTGCTCAAGTATTCGCAGAGATCGAAGGATGTTGACATAGTTTTGGAAAGGATGGCAAAGAACAAGTGTGAAATGACAAGCGATTTGTACAATTTGATGTTTAGGTTATATGTGCAATGGGACAAGGAAGAGAAAGTGAGAGAGATATGGAGTGAGATGGAGAGAAGTGGATTAGGACCAGATCAGAGAACATATACTATTAGGATTCATGGTCTGCACACAAAGGGGAAAATTGGAGAAGCATTGAGTTACTTTCAAGAGATGATGTCAAAAGGAATGGTACCAGAGCCAAGAACTGAGATGTTACTGAACCAGAACAAGACCAAGCCAAGAGTAGAAGACAAAATGCTTAGATCGAACTTAACAAGCGAAGAGTCTGAAAGCGACTAA

Protein Analysis

523

Amino Acids

61.08

Weight (kDa)

8.94

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 143 - 189 5e-07 PPR repeat family
PPR_2 PF13041 246 - 294 3.7e-09 PPR repeat family
PPR_1 PF12854 278 - 308 1.1e-07 PPR repeat
PPR_2 PF13041 280 - 317 8.2e-07 PPR repeat family
PPR_2 PF13041 315 - 364 7.4e-14 PPR repeat family
PPR_3 PF13812 315 - 361 5.9e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 339 - 397 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 346 - 379 1.1e-06 PPR repeat
PPR_2 PF13041 350 - 397 1.6e-12 PPR repeat family
PPR_2 PF13041 423 - 466 5.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020990)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15200
malus_domestica MD03G1231900.v1.1 MD11G1253000.v1.1
prunus_persica Prupe.4G176100_v2.0.a1
pyrus_communis pycom03g17930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1462
AccB1I GGYRCC 2 cut(s) 57, 1462
AccB7I CCANNNNNTGG 1 cut(s) 151
AccII CGCG 1 cut(s) 55
AciI CCGC 5 cut(s) 55, 292, 309, 469, 695
AclI AACGTT 1 cut(s) 966
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 2 cut(s) 191, 689
AcyI GRCGYC 1 cut(s) 58
AdeI CACNNNGTG 1 cut(s) 493
AfaI GTAC 4 cut(s) 157, 542, 1271, 1464
AfiI CCNNNNNNNGG 1 cut(s) 151
AgsI TTSAA 8 cut(s) 23, 208, 479, 689, 988, 1013, 1078, 1439
AhdI GACNNNNNGTC 1 cut(s) 505
AjnI CCWGG 1 cut(s) 137
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AluBI AGCT 9 cut(s) 37, 182, 331, 377, 426, 590, 797, 852, 1120
AluI AGCT 9 cut(s) 37, 182, 331, 377, 426, 590, 797, 852, 1120
Alw21I GWGCWC 2 cut(s) 49, 184
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 836
ApeKI GCWGC 2 cut(s) 566, 569
ApoI RAATTY 2 cut(s) 191, 689
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 1462
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 2 cut(s) 1040, 1358
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 3 cut(s) 254, 267, 479
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 2 cut(s) 1040, 1358
AxyI CCTNAGG 1 cut(s) 1002
BanI GGYRCC 2 cut(s) 57, 1462
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 542, 1527
Bbv12I GWGCWC 2 cut(s) 49, 184
BbvI GCAGC 2 cut(s) 553, 556
BccI CCATC 4 cut(s) 512, 1126, 1228, 1333
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 139
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 2 cut(s) 423, 1121
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 4 cut(s) 56, 470, 567, 570
BlsI GCNGC 4 cut(s) 57, 471, 568, 571
Bme1390I CCNGG 2 cut(s) 139, 944
Bme18I GGWCC 2 cut(s) 1040, 1358
BmeRI GACNNNNNGTC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 1040, 1358
BmiI GGNNCC 4 cut(s) 59, 143, 1042, 1464
BmrFI CCNGG 2 cut(s) 139, 944
BmsI GCATC 3 cut(s) 268, 964, 1090
BpiI GAAGAC 2 cut(s) 542, 1527
BpmI CTGGAG 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 1172
BpuMI CCSGG 1 cut(s) 944
Bsa29I ATCGAT 1 cut(s) 358
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse21I CCTNAGG 1 cut(s) 1002
Bse3DI GCAATG 3 cut(s) 819, 927, 1304
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 1 cut(s) 139
BseCI ATCGAT 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 3 cut(s) 952, 1216, 1239
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMI GCAATG 3 cut(s) 819, 927, 1304
BseMII CTCAG 3 cut(s) 993, 1095, 1470
BseRI GAGGAG 1 cut(s) 622
BseXI GCAGC 2 cut(s) 553, 556
BsgI GTGCAG 2 cut(s) 555, 1382
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 1 cut(s) 838
BshNI GGYRCC 2 cut(s) 57, 1462
BshVI ATCGAT 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 49, 184
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 2 cut(s) 61, 944
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 4 cut(s) 871, 941, 1026, 1316
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 4 cut(s) 871, 941, 1026, 1316
BsnI GGCC 1 cut(s) 838
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 49, 184
Bsp1407I TGTACA 1 cut(s) 1269
Bsp143I GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
BspACI CCGC 5 cut(s) 55, 292, 309, 469, 695
BspANI GGCC 1 cut(s) 838
BspCNI CTCAG 3 cut(s) 994, 1096, 1471
BspDI ATCGAT 1 cut(s) 358
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 4 cut(s) 59, 143, 1042, 1464
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 57, 1462
BsrDI GCAATG 3 cut(s) 819, 927, 1304
BsrGI TGTACA 1 cut(s) 1269
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
BssNI GRCGYC 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 266, 560
Bst6I CTCTTC 7 cut(s) 321, 474, 715, 986, 1122, 1305, 1548
BstACI GRCGYC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 1269
BstC8I GCNNGC 1 cut(s) 836
BstDEI CTNAG 6 cut(s) 510, 798, 1002, 1104, 1479, 1532
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 3 cut(s) 952, 1216, 1239
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 6 cut(s) 66, 362, 637, 1205, 1366, 1538
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 2 cut(s) 137, 942
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 553, 556
BstV2I GAAGAC 2 cut(s) 542, 1527
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
Bsu15I ATCGAT 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 1002
BsuRI GGCC 1 cut(s) 838
BsuTUI ATCGAT 1 cut(s) 358
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 761
BtsCI GGATG 3 cut(s) 952, 1216, 1239
Cac8I GCNNGC 1 cut(s) 836
CciI TCATGA 1 cut(s) 790
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 2 cut(s) 1040, 1358
ClaI ATCGAT 1 cut(s) 358
Csp6I GTAC 4 cut(s) 156, 541, 1270, 1463
CviAII CATG 4 cut(s) 650, 683, 791, 1391
CviQI GTAC 4 cut(s) 156, 541, 1270, 1463
DdeI CTNAG 6 cut(s) 510, 798, 1002, 1104, 1479, 1532
DinI GGCGCC 1 cut(s) 59
DpnI GATC 6 cut(s) 65, 361, 636, 1204, 1365, 1537
DpnII GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
DraIII CACNNNGTG 1 cut(s) 493
DriI GACNNNNNGTC 1 cut(s) 505
Eam1104I CTCTTC 7 cut(s) 321, 474, 715, 986, 1122, 1305, 1548
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 7 cut(s) 321, 474, 715, 986, 1122, 1305, 1548
Ecl136II GAGCTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 184
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 2 cut(s) 1040, 1358
Eco53kI GAGCTC 1 cut(s) 182
Eco81I CCTNAGG 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 1040
EcoRII CCWGG 1 cut(s) 137
EcoRV GATATC 1 cut(s) 817
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 184
EgeI GGCGCC 1 cut(s) 59
EheI GGCGCC 1 cut(s) 59
FaeI CATG 4 cut(s) 653, 686, 794, 1394
FaqI GGGAC 4 cut(s) 871, 941, 1026, 1316
FatI CATG 4 cut(s) 649, 682, 790, 1390
FauI CCCGC 1 cut(s) 302
Fnu4HI GCNGC 4 cut(s) 56, 470, 567, 570
FokI GGATG 3 cut(s) 959, 1223, 1246
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 56, 470, 567, 570
FspBI CTAG 2 cut(s) 423, 1121
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 56, 470, 567, 570
GsuI CTGGAG 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 1 cut(s) 838
HapII CCGG 2 cut(s) 61, 944
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 4 cut(s) 653, 686, 794, 1394
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 529, 1216
HindII GTYRAC 2 cut(s) 529, 1216
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 2 cut(s) 61, 944
HphI GGTGA 3 cut(s) 254, 267, 479
Hpy166II GTNNAC 4 cut(s) 158, 275, 529, 1216
Hpy188I TCNGA 4 cut(s) 354, 364, 1368, 1561
Hpy8I GTNNAC 4 cut(s) 158, 275, 529, 1216
Hpy99I CGWCG 2 cut(s) 215, 503
HpyAV CCTTC 2 cut(s) 877, 1201
HpyCH4III ACNGT 2 cut(s) 266, 560
HpyCH4IV ACGT 3 cut(s) 539, 966, 1061
HpyCH4V TGCA 7 cut(s) 572, 834, 872, 960, 1089, 1297, 1399
HpyF3I CTNAG 6 cut(s) 510, 798, 1002, 1104, 1479, 1532
HpySE526I ACGT 3 cut(s) 539, 966, 1061
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 4 cut(s) 653, 686, 794, 1394
HspAI GCGC 1 cut(s) 58
KasI GGCGCC 1 cut(s) 57
KpnI GGTACC 1 cut(s) 1466
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 3 cut(s) 179, 187, 1117
Lsp1109I GCAGC 2 cut(s) 553, 556
LweI GCATC 3 cut(s) 268, 964, 1090
MaeI CTAG 2 cut(s) 423, 1121
MaeII ACGT 3 cut(s) 539, 966, 1061
MaeIII GTNAC 6 cut(s) 560, 1057, 1168, 1178, 1430, 1485
MalI GATC 6 cut(s) 65, 361, 636, 1204, 1365, 1537
MboI GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 2 cut(s) 49, 184
MluCI AATT 8 cut(s) 18, 191, 392, 689, 890, 908, 1273, 1413
Mly113I GGCGCC 1 cut(s) 58
MlyI GAGTC 2 cut(s) 606, 1565
MnlI CCTC 5 cut(s) 108, 600, 603, 795, 997
Mph1103I ATGCAT 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 9 cut(s) 288, 320, 348, 402, 528, 554, 951, 1140, 1544
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 2 cut(s) 61, 944
MspR9I CCNGG 2 cut(s) 139, 944
MunI CAATTG 1 cut(s) 392
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 55
NarI GGCGCC 1 cut(s) 58
NciI CCSGG 1 cut(s) 944
NdeII GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
NlaIII CATG 4 cut(s) 653, 686, 794, 1394
NlaIV GGNNCC 4 cut(s) 59, 143, 1042, 1464
NmuCI GTSAC 2 cut(s) 1057, 1168
NsiI ATGCAT 1 cut(s) 874
NspI RCATGY 1 cut(s) 686
PagI TCATGA 1 cut(s) 790
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 447, 1202
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 8 cut(s) 112, 204, 295, 355, 445, 757, 996, 1387
Pfl23II CGTACG 1 cut(s) 540
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 4 cut(s) 57, 471, 568, 571
PleI GAGTC 2 cut(s) 605, 1564
PluTI GGCGCC 1 cut(s) 61
PpsI GAGTC 2 cut(s) 605, 1564
PpuMI RGGWCCY 1 cut(s) 1040
Psp124BI GAGCTC 1 cut(s) 184
Psp1406I AACGTT 1 cut(s) 966
Psp5II RGGWCCY 1 cut(s) 1040
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 4 cut(s) 59, 143, 1042, 1464
PspPI GGNCC 2 cut(s) 1040, 1358
PspPPI RGGWCCY 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 4 cut(s) 157, 542, 1271, 1464
RsaNI GTAC 4 cut(s) 156, 541, 1270, 1463
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 9 cut(s) 288, 320, 348, 402, 528, 554, 951, 1140, 1544
SatI GCNGC 4 cut(s) 56, 470, 567, 570
Sau3AI GATC 6 cut(s) 63, 359, 634, 1202, 1363, 1535
Sau96I GGNCC 2 cut(s) 1040, 1358
SchI GAGTC 2 cut(s) 606, 1565
ScrFI CCNGG 2 cut(s) 139, 944
SduI GDGCHC 2 cut(s) 49, 184
SfaNI GCATC 3 cut(s) 268, 964, 1090
SfcI CTRYAG 1 cut(s) 591
SfoI GGCGCC 1 cut(s) 59
SinI GGWCC 2 cut(s) 1040, 1358
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
Sse9I AATT 8 cut(s) 18, 191, 392, 689, 890, 908, 1273, 1413
SsiI CCGC 5 cut(s) 55, 292, 309, 469, 695
SspDI GGCGCC 1 cut(s) 57
SspMI CTAG 2 cut(s) 423, 1121
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 137, 942
TaaI ACNGT 2 cut(s) 266, 560
TaiI ACGT 3 cut(s) 542, 969, 1064
TaqI TCGA 8 cut(s) 78, 300, 358, 428, 450, 498, 1205, 1538
TasI AATT 8 cut(s) 18, 191, 392, 689, 890, 908, 1273, 1413
TatI WGTACW 1 cut(s) 1269
TauI GCSGC 2 cut(s) 58, 472
TfiI GAWTC 8 cut(s) 112, 204, 295, 355, 445, 757, 996, 1387
Tru1I TTAA 9 cut(s) 288, 320, 348, 402, 528, 554, 951, 1140, 1544
Tru9I TTAA 9 cut(s) 288, 320, 348, 402, 528, 554, 951, 1140, 1544
TseFI GTSAC 2 cut(s) 1057, 1168
TseI GCWGC 2 cut(s) 566, 569
Tsp45I GTSAC 2 cut(s) 1057, 1168
TspDTI ATGAA 9 cut(s) 158, 233, 404, 600, 638, 764, 779, 807, 1379
Van91I CCANNNNNTGG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 1040, 1358
XapI RAATTY 2 cut(s) 191, 689
XceI RCATGY 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 423, 1121
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.