AT3G44150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0021887
Physical Location & Seq
Forward (+)
15887490 .. 15888879
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44150.1

Sequence Viewer

Length: 741 bp
ATGTTCTCTTCGAAAACCCTAACACTCCTCGTCTTCTCCGCCGTGATTCTAACCGTAGCCTTAGGCGGAGACAGCGGAGGTTCAGGTAACACCAACACTATCTATTCACCATGCTCCGACACCCGAATCCAAAGATCCGATGGATTCACATTCGGTATCGCATTCTCTTCTCGTCCCTCCTTCTTCATAAACCAAACGGTTCTTCTCTCTCCCTGTGACCGCCGCCTTTCCCTCGCCGCCATGAATTCACAGTTCTCTGTTTTCCGTCCTAAAATCGACGAGATCTCTCTCCTCTCCATCAACACCTCCGCCTTCTTCCCGGACAACTATGGTGGATATATGGTAGCATTCGCGGGTCGAAAATACGCAGCGAGGTCTATACCGGCTTTTATTGCTAATAGCACCTTCATCGTAACCAGTTTTACTTTGGTGATGGAGTTTCAGAAAGGTAGGCTTCAGAATCTGTATTGGAAGAGAGACGGGTGTGCGTCGTGTAAAGGGAATCAAAATTTTGTGTGTCTTAATAAGCAAGATTGTGCGATAAGAACACCGAGCTGTAAAGGTCGGGGAGGTGCCGTGGACTGTAGTCTCGGGATACAGCTGGCGTTTTCCGGGACTGATAAGCACTTGGCGGTTCTCAACTCGTGGTATGAAGTTGAGAATCTTAAGCAGTATTCTCTTTATGGTTTGTATTCAAACCTCAAGAGCTCTTTAACCAATCAGTTCAATAATTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.09

Weight (kDa)

9.23

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44150
malus_domestica MD03G1158900.v1.1 MD11G1174300.v1.1
pyrus_communis pycom03g11060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 572
AccII CGCG 1 cut(s) 353
AciI CCGC 9 cut(s) 39, 66, 75, 220, 223, 237, 309, 353, 632
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 2 cut(s) 244, 508
AcuI CTGAAG 1 cut(s) 440
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 2 cut(s) 696, 727
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AluBI AGCT 3 cut(s) 555, 601, 708
AluI AGCT 3 cut(s) 555, 601, 708
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 3 cut(s) 63, 471, 593
AlwI GGATC 1 cut(s) 129
AlwNI CAGNNNCTG 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 590
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 2 cut(s) 244, 508
AsuC2I CCSGG 2 cut(s) 320, 613
AsuHPI GGTGA 2 cut(s) 99, 442
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 590
AxyI CCTNAGG 1 cut(s) 61
BanI GGYRCC 1 cut(s) 572
BanII GRGCYC 1 cut(s) 710
BauI CACGAG 1 cut(s) 643
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 710
BbvI GCAGC 1 cut(s) 380
BccI CCATC 3 cut(s) 134, 305, 427
BceAI ACGGC 2 cut(s) 26, 560
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciVI GTATCC 1 cut(s) 588
BcnI CCSGG 2 cut(s) 320, 613
BcoDI GTCTC 3 cut(s) 63, 471, 593
BfmI CTRYAG 1 cut(s) 583
BfrI CTTAAG 1 cut(s) 665
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 1 cut(s) 282
BisI GCNGC 3 cut(s) 223, 237, 369
BlsI GCNGC 3 cut(s) 224, 238, 370
Bme1390I CCNGG 2 cut(s) 320, 613
BmeT110I CYCGRG 1 cut(s) 590
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 2 cut(s) 320, 613
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 2 cut(s) 320, 613
BsaJI CCNNGG 1 cut(s) 576
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 417
Bse21I CCTNAGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 576
BseNI ACTGG 1 cut(s) 417
BseRI GAGGAG 2 cut(s) 17, 281
BseXI GCAGC 1 cut(s) 380
Bsh1236I CGCG 1 cut(s) 353
BshNI GGYRCC 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 710
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 320, 383, 612
BslFI GGGAC 2 cut(s) 159, 628
BsmAI GTCTC 3 cut(s) 63, 471, 593
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 159, 628
BsmI GAATGC 2 cut(s) 161, 347
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 710
Bsp143I GATC 2 cut(s) 134, 282
BspACI CCGC 9 cut(s) 39, 66, 75, 220, 223, 237, 309, 353, 632
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 574
BspPI GGATC 1 cut(s) 129
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 1 cut(s) 417
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 2 cut(s) 134, 282
BssSI CACGAG 1 cut(s) 643
Bst2BI CACGAG 1 cut(s) 643
Bst4CI ACNGT 4 cut(s) 55, 199, 252, 584
Bst6I CTCTTC 3 cut(s) 13, 172, 467
BstAFI CTTAAG 1 cut(s) 665
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 1 cut(s) 61
BstDSI CCRYGG 1 cut(s) 576
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 2 cut(s) 137, 285
BstMAI GTCTC 3 cut(s) 63, 471, 593
BstMBI GATC 2 cut(s) 134, 282
BstMWI GCNNNNNNNGC 2 cut(s) 72, 392
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 318, 611
BstSFI CTRYAG 1 cut(s) 583
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 380
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 134, 282
BstYI RGATCY 2 cut(s) 134, 282
Bsu36I CCTNAGG 1 cut(s) 61
BsuI GTATCC 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 576
Cac8I GCNNGC 1 cut(s) 603
CaiI CAGNNNCTG 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 382
CseI GACGC 1 cut(s) 477
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 2 cut(s) 111, 241
CviJI RGCY 6 cut(s) 59, 386, 454, 555, 601, 708
CviKI_1 RGCY 6 cut(s) 59, 386, 454, 555, 601, 708
DdeI CTNAG 1 cut(s) 61
DpnI GATC 2 cut(s) 136, 284
DpnII GATC 2 cut(s) 134, 282
Eam1104I CTCTTC 3 cut(s) 13, 172, 467
EarI CTCTTC 3 cut(s) 13, 172, 467
EciI GGCGGA 3 cut(s) 28, 81, 298
Ecl136II GAGCTC 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 710
Eco53kI GAGCTC 1 cut(s) 708
Eco57I CTGAAG 1 cut(s) 440
Eco81I CCTNAGG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 2 cut(s) 114, 244
FaiI YATR 9 cut(s) 112, 188, 242, 330, 339, 341, 380, 651, 684
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 2 cut(s) 159, 628
FatI CATG 2 cut(s) 110, 240
FauI CCCGC 1 cut(s) 346
Fnu4HI GCNGC 3 cut(s) 223, 237, 369
FriOI GRGCYC 1 cut(s) 710
Fsp4HI GCNGC 3 cut(s) 223, 237, 369
GluI GCNGC 3 cut(s) 223, 237, 369
HapII CCGG 3 cut(s) 320, 383, 612
HgaI GACGC 1 cut(s) 477
Hin1II CATG 2 cut(s) 114, 244
HinfI GANTC 6 cut(s) 46, 126, 144, 460, 502, 661
HpaII CCGG 3 cut(s) 320, 383, 612
HphI GGTGA 2 cut(s) 99, 442
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 4 cut(s) 118, 139, 444, 459
Hpy188III TCNNGA 2 cut(s) 592, 703
Hpy8I GTNNAC 1 cut(s) 580
Hpy99I CGWCG 2 cut(s) 281, 493
HpyAV CCTTC 3 cut(s) 190, 322, 415
HpyCH4III ACNGT 4 cut(s) 55, 199, 252, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 392
HpyF3I CTNAG 1 cut(s) 61
Hsp92II CATG 2 cut(s) 114, 244
Kzo9I GATC 2 cut(s) 134, 282
LmnI GCTCC 1 cut(s) 119
LpnPI CCDG 7 cut(s) 69, 226, 333, 396, 430, 587, 625
Lsp1109I GCAGC 1 cut(s) 380
MaeIII GTNAC 3 cut(s) 86, 215, 412
MalI GATC 2 cut(s) 136, 284
MboI GATC 2 cut(s) 134, 282
MboII GAAGA 6 cut(s) 25, 159, 175, 194, 307, 484
MflI RGATCY 2 cut(s) 134, 282
MhlI GDGCHC 1 cut(s) 710
MluCI AATT 3 cut(s) 244, 508, 730
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 9 cut(s) 38, 71, 187, 242, 302, 316, 366, 563, 710
MseI TTAA 3 cut(s) 522, 666, 713
MspA1I CMGCKG 2 cut(s) 75, 601
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 3 cut(s) 320, 383, 612
MspR9I CCNGG 2 cut(s) 320, 613
Mva1269I GAATGC 2 cut(s) 161, 347
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 72, 392
NciI CCSGG 2 cut(s) 320, 613
NdeII GATC 2 cut(s) 134, 282
NlaIII CATG 2 cut(s) 114, 244
NlaIV GGNNCC 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 215
NspV TTCGAA 1 cut(s) 11
PctI GAATGC 2 cut(s) 161, 347
PfeI GAWTC 6 cut(s) 46, 126, 144, 460, 502, 661
PfoI TCCNGGA 2 cut(s) 318, 611
PkrI GCNGC 3 cut(s) 224, 238, 370
PshAI GACNNNNGTC 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 710
PspN4I GGNNCC 1 cut(s) 574
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 2 cut(s) 134, 282
PvuII CAGCTG 1 cut(s) 601
SacI GAGCTC 1 cut(s) 710
SaqAI TTAA 3 cut(s) 522, 666, 713
SatI GCNGC 3 cut(s) 223, 237, 369
Sau3AI GATC 2 cut(s) 134, 282
ScrFI CCNGG 2 cut(s) 320, 613
SduI GDGCHC 1 cut(s) 710
SfcI CTRYAG 1 cut(s) 583
SfuI TTCGAA 1 cut(s) 11
SmlI CTYRAG 2 cut(s) 665, 701
SmoI CTYRAG 2 cut(s) 665, 701
Sse9I AATT 3 cut(s) 244, 508, 730
SsiI CCGC 9 cut(s) 39, 66, 75, 220, 223, 237, 309, 353, 632
SstI GAGCTC 1 cut(s) 710
StyD4I CCNGG 2 cut(s) 318, 611
TaaI ACNGT 4 cut(s) 55, 199, 252, 584
TaqI TCGA 3 cut(s) 11, 276, 358
TasI AATT 3 cut(s) 244, 508, 730
TauI GCSGC 2 cut(s) 225, 239
TfiI GAWTC 6 cut(s) 46, 126, 144, 460, 502, 661
Tru1I TTAA 3 cut(s) 522, 666, 713
Tru9I TTAA 3 cut(s) 522, 666, 713
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 4 cut(s) 175, 257, 397, 666
TspGWI ACGGA 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 665
XapI RAATTY 2 cut(s) 244, 508
XcmI CCANNNNNNNNNTGG 2 cut(s) 137, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.