MD11G1174300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0021887
Physical Location & Seq
Reverse (-)
20430673 .. 20433810
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1174300.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCAATCACCGGCGGCAACAATGGCGGCGGCGATGCTCCTCCTCCTCCTCCTGCTGATGGCGTCGATTGTGGTGACTGTGGGAGCAGCCAACACAAACAGCGTGTACAGTCCATGCTCAGACACCAAGATTCAGAGGTCCGACGGCTTCACATTCGGGATCGCATTCTCGTCCAAGAACTCCTTCTTCCTAAACGGGAACCAGTCGCATCAGCTCTCCCCCTGCGACCGCCGCCTCTCGCTGTCCTCCGCCAACTCCCAGCTCGCGCTCTTTAGACCCAAAGTCGATGAGATCTCCCTCCTCACTATCAACACCTCTTCTTTCCAACCGGATTCATATGGTGGATACATGGTGGCGTTCGCTGGACGAAAATATGCTGCAAGATCTCTCCCTGCCTTTGTTGCAAACAGCACATACATAGTGACAAGTTTTACCTTGGTACTCGAGTTTCAAAGGGGTAGGCTGCAAAACTTGTATTGGAAGAGAGATGGGTGTGCCAAATGCACAGGAAACTCCAATTTTGTTTGTCTTAACAAACAAGACTGCGCCATTAAAATGTCAAGCTGTAAAAACCGAGGGGGTTCTGTAGATTGCAGTCTCGGGATACAACTGGCTTTCTCCGGTACAGATAAACATCTGTCGGTTCTCAATTCATGGTATGAGGTGGAGAACCTTCGGCAGTATTCTCTCTATGGGTTGTATTCCAATCTCAGAGATTCTCTCACTAGCCAGTATAACAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.28

Weight (kDa)

9.21

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44150
malus_domestica MD03G1158900.v1.1 MD11G1174300.v1.1
pyrus_communis pycom03g11060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 6 cut(s) 14, 26, 29, 229, 232, 249
AclWI GGATC 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 3 cut(s) 107, 441, 625
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 452
AhdI GACNNNNNGTC 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 3 cut(s) 214, 262, 564
AluI AGCT 3 cut(s) 214, 262, 564
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 1 cut(s) 167
Ama87I CYCGRG 2 cut(s) 443, 599
ApeKI GCWGC 3 cut(s) 86, 377, 463
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 2 cut(s) 268, 548
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 2 cut(s) 443, 599
AvaII GGWCC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 431, 464
BbvI GCAGC 3 cut(s) 98, 364, 450
BccI CCATC 2 cut(s) 52, 482
BceAI ACGGC 1 cut(s) 160
BciVI GTATCC 2 cut(s) 338, 597
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 585
BfuI GTATCC 2 cut(s) 338, 597
BglII AGATCT 2 cut(s) 291, 383
BisI GCNGC 7 cut(s) 15, 27, 30, 87, 232, 378, 464
BlsI GCNGC 7 cut(s) 16, 28, 31, 88, 233, 379, 465
Bme18I GGWCC 1 cut(s) 138
BmeRI GACNNNNNGTC 1 cut(s) 281
BmeT110I CYCGRG 2 cut(s) 443, 599
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 200
BmsI GCATC 2 cut(s) 24, 217
BplI GAGNNNNNCTC 2 cut(s) 705, 737
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 435, 574
BsaWI WCCGGW 2 cut(s) 328, 620
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 3 cut(s) 202, 615, 730
Bse8I GATNNNNATC 1 cut(s) 633
BseDI CCNNGG 2 cut(s) 435, 574
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 2 cut(s) 132, 724
BseNI ACTGG 3 cut(s) 202, 615, 730
BseRI GAGGAG 5 cut(s) 29, 32, 35, 38, 290
BseXI GCAGC 3 cut(s) 98, 364, 450
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 229
BsiEI CGRYCG 1 cut(s) 229
BsiHKCI CYCGRG 2 cut(s) 443, 599
BsiSI CCGG 3 cut(s) 11, 329, 621
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 164
BsoBI CYCGRG 2 cut(s) 443, 599
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 3 cut(s) 159, 291, 383
BspACI CCGC 6 cut(s) 14, 26, 29, 229, 232, 249
BspCNI CTCAG 2 cut(s) 131, 723
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 200
BspPI GGATC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 3 cut(s) 202, 615, 730
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 435, 574
BssMI GATC 3 cut(s) 159, 291, 383
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 79, 110
Bst6I CTCTTC 2 cut(s) 322, 476
BstACI GRCGYC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 2 cut(s) 118, 710
BstFNI CGCG 1 cut(s) 266
BstHHI GCGC 2 cut(s) 268, 548
BstKTI GATC 3 cut(s) 162, 294, 386
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 3 cut(s) 159, 291, 383
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 3 cut(s) 23, 231, 401
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 3 cut(s) 98, 364, 450
BstX2I RGATCY 2 cut(s) 291, 383
BstYI RGATCY 2 cut(s) 291, 383
BsuI GTATCC 2 cut(s) 338, 597
BtgZI GCGATG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 2 cut(s) 268, 548
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 138
CseI GACGC 1 cut(s) 51
Csp6I GTAC 3 cut(s) 106, 440, 624
CviAII CATG 3 cut(s) 114, 349, 654
CviJI RGCY 8 cut(s) 89, 147, 214, 262, 463, 564, 614, 729
CviKI_1 RGCY 8 cut(s) 89, 147, 214, 262, 463, 564, 614, 729
CviQI GTAC 3 cut(s) 106, 440, 624
DdeI CTNAG 2 cut(s) 118, 710
DpnI GATC 3 cut(s) 161, 293, 385
DpnII GATC 3 cut(s) 159, 291, 383
DriI GACNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 2 cut(s) 322, 476
Eam1105I GACNNNNNGTC 1 cut(s) 281
EarI CTCTTC 2 cut(s) 322, 476
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 138
Eco88I CYCGRG 2 cut(s) 443, 599
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 3 cut(s) 117, 352, 657
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FatI CATG 3 cut(s) 113, 348, 653
FauNDI CATATG 1 cut(s) 337
Fnu4HI GCNGC 7 cut(s) 15, 27, 30, 87, 232, 378, 464
Fsp4HI GCNGC 7 cut(s) 15, 27, 30, 87, 232, 378, 464
FspBI CTAG 1 cut(s) 726
GlaI GCGC 2 cut(s) 267, 547
GluI GCNGC 7 cut(s) 15, 27, 30, 87, 232, 378, 464
GsaI CCCAGC 1 cut(s) 262
HapII CCGG 3 cut(s) 11, 329, 621
HgaI GACGC 1 cut(s) 51
HhaI GCGC 2 cut(s) 268, 548
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 3 cut(s) 117, 352, 657
Hin6I GCGC 2 cut(s) 266, 546
HinP1I GCGC 2 cut(s) 266, 546
HinfI GANTC 3 cut(s) 130, 332, 716
HpaII CCGG 3 cut(s) 11, 329, 621
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 121, 135, 142, 713
Hpy188III TCNNGA 2 cut(s) 157, 601
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 2 cut(s) 67, 146
HpyAV CCTTC 2 cut(s) 193, 683
HpyCH4III ACNGT 2 cut(s) 79, 110
HpyCH4V TGCA 6 cut(s) 4, 380, 404, 466, 504, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 231, 401
HpyF3I CTNAG 2 cut(s) 118, 710
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 3 cut(s) 117, 352, 657
HspAI GCGC 2 cut(s) 266, 546
Kzo9I GATC 3 cut(s) 159, 291, 383
LmnI GCTCC 2 cut(s) 42, 83
Lsp1109I GCAGC 3 cut(s) 98, 364, 450
LweI GCATC 2 cut(s) 24, 217
MaeI CTAG 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 73, 421
MalI GATC 3 cut(s) 161, 293, 385
MboI GATC 3 cut(s) 159, 291, 383
MboII GAAGA 3 cut(s) 178, 309, 493
MflI RGATCY 2 cut(s) 291, 383
MluCI AATT 2 cut(s) 517, 649
MmeI TCCRAC 2 cut(s) 165, 349
MroXI GAANNNNTTC 1 cut(s) 182
MseI TTAA 2 cut(s) 531, 552
MslI CAYNNNNRTG 1 cut(s) 554
MspI CCGG 3 cut(s) 11, 329, 621
Mva1269I GAATGC 1 cut(s) 164
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 3 cut(s) 23, 231, 401
NdeI CATATG 1 cut(s) 337
NdeII GATC 3 cut(s) 159, 291, 383
NlaIII CATG 3 cut(s) 117, 352, 657
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 73, 421
PaeR7I CTCGAG 1 cut(s) 443
PctI GAATGC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 3 cut(s) 130, 332, 716
PkrI GCNGC 7 cut(s) 16, 28, 31, 88, 233, 379, 465
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 1 cut(s) 138
PspXI VCTCGAGB 1 cut(s) 443
PsrI GAACNNNNNNTAC 1 cut(s) 725
PsuI RGATCY 2 cut(s) 291, 383
RsaI GTAC 3 cut(s) 107, 441, 625
RsaNI GTAC 3 cut(s) 106, 440, 624
RseI CAYNNNNRTG 1 cut(s) 554
SaqAI TTAA 2 cut(s) 531, 552
SatI GCNGC 7 cut(s) 15, 27, 30, 87, 232, 378, 464
Sau3AI GATC 3 cut(s) 159, 291, 383
Sau96I GGNCC 1 cut(s) 138
SetI ASST 8 cut(s) 140, 216, 264, 317, 437, 566, 666, 675
SfaNI GCATC 2 cut(s) 24, 217
SfcI CTRYAG 1 cut(s) 585
Sfr274I CTCGAG 1 cut(s) 443
SgrAI CRCCGGYG 1 cut(s) 10
SinI GGWCC 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 2 cut(s) 517, 649
SsiI CCGC 6 cut(s) 14, 26, 29, 229, 232, 249
SspMI CTAG 1 cut(s) 726
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 79, 110
TaqI TCGA 3 cut(s) 65, 285, 444
TasI AATT 2 cut(s) 517, 649
TatI WGTACW 1 cut(s) 105
TauI GCSGC 4 cut(s) 17, 29, 32, 234
TfiI GAWTC 3 cut(s) 130, 332, 716
Tru1I TTAA 2 cut(s) 531, 552
Tru9I TTAA 2 cut(s) 531, 552
TseFI GTSAC 2 cut(s) 73, 421
TseI GCWGC 3 cut(s) 86, 377, 463
Tsp45I GTSAC 2 cut(s) 73, 421
TspDTI ATGAA 2 cut(s) 324, 642
VpaK11BI GGWCC 1 cut(s) 138
XhoI CTCGAG 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.