AT3G54180
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014914
Physical Location & Seq
Forward (+)
20059671 .. 20061488
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54180.1

Sequence Viewer

Length: 930 bp
ATGGAGAAGTACGAGAAGCTAGAGAAGGTCGGAGAAGGAACATACGGGAAAGTCTACAAAGCGATGGAGAAAGGAACTGGTAAGCTTGTTGCTCTGAAGAAAACTCGTCTCGAGATGGACGAAGAAGGTATTCCACCAACTGCTCTTCGTGAGATCTCGCTTCTCCAGATGTTATCAACATCGATCTATGTTGTTCGATTACTCTGCGTCGAACATGTTCATCAACCATCAACCAAATCTCAATCTACCAAATCCAATCTCTATCTCGTTTTCGAGTATCTCGATACTGATCTTAAGAAATTCATCGATTCGTATAGGAAAGGACCTAATCCTAAGCCTCTTGAGCCTTTTTTGATTCAGAAGTTGATGTTTCAGCTTTGTAAAGGTGTTGCGCATTGTCATAGTCATGGTGTGCTTCACCGTGATCTTAAACCGCAGAATCTTCTTCTGGTGAAAGATAAAGAGCTTCTTAAGATTGCTGATTTGGGTCTTGGTCGTGCTTTTACTGTTCCTCTTAAGTCTTATACGCATGAGATTGTTACTCTTTGGTATAGAGCTCCTGAAGTTCTTCTTGGATCTACTCATTATTCAACTGGTGTTGACATGTGGTCTGTTGGTTGTATCTTTGCTGAGATGGTTCGGAGGCAAGCTCTTTTCCCTGGTGATTCTGAGTTTCAGCAATTGCTTCATATCTTCAGGTTGCTAGGAACACCAACTGAGCAGCAATGGCCGGGTGTTTCCACACTGCGTGACTGGCATGTTTACCCTAAGTGGGAGCCGCAAGACTTAACTCTTGCTGTTCCTTCTCTTTCACCTCAAGGAGTTGATCTTCTCACGAAAATGCTCAAGTACAATCCAGCCGAAAGAATTTCAGCAAAAACAGCACTTGATCACCCATATTTTGACAGCCTTGACAAGTCTCAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

309

Amino Acids

35.32

Weight (kDa)

8.67

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 301 3.5e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 7 - 215 7.4e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38620 AT2G38620 AT2G38620 AT2G38620 AT3G54180
fragaria_vesca FvH4_6g09190
malus_domestica MD12G1195200.v1.1
prunus_persica Prupe.6G299900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0459391
rosa_laevigata RLG00000025084
rosa_multiflora Rmu_sc0025882.1_g000001
rosa_roxburghii Rroxscaffold_6G00420190
rosa_rugosa Rorug03G0036200
rosa_samantha Rh3AG094100 Rh3DG097800
rosa_wichuraiana Rw3G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 393
AccI GTMKAC 1 cut(s) 54
AciI CCGC 2 cut(s) 434, 779
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 2 cut(s) 299, 867
AcuI CTGAAG 3 cut(s) 116, 582, 679
AdeI CACNNNGTG 1 cut(s) 749
AfaI GTAC 2 cut(s) 11, 851
AfiI CCNNNNNNNGG 1 cut(s) 772
AflII CTTAAG 3 cut(s) 293, 470, 515
AflIII ACRYGT 2 cut(s) 214, 603
AgsI TTSAA 1 cut(s) 591
AhdI GACNNNNNGTC 1 cut(s) 607
AjnI CCWGG 1 cut(s) 658
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 6 cut(s) 19, 85, 376, 466, 557, 650
AluI AGCT 6 cut(s) 19, 85, 376, 466, 557, 650
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 2 cut(s) 113, 924
AlwI GGATC 1 cut(s) 583
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 1 cut(s) 728
ApeKI GCWGC 1 cut(s) 721
ApoI RAATTY 2 cut(s) 299, 867
Asp700I GAANNNNTTC 3 cut(s) 129, 216, 567
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 1 cut(s) 732
AsuHPI GGTGA 5 cut(s) 410, 463, 674, 804, 884
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 559
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 1 cut(s) 733
BccI CCATC 4 cut(s) 58, 109, 235, 628
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 1 cut(s) 732
BcoDI GTCTC 2 cut(s) 113, 924
BfaI CTAG 2 cut(s) 20, 704
BfrI CTTAAG 3 cut(s) 293, 470, 515
BglII AGATCT 1 cut(s) 153
BisI GCNGC 2 cut(s) 722, 779
BlsI GCNGC 2 cut(s) 723, 780
Bme1390I CCNGG 2 cut(s) 660, 732
Bme18I GGWCC 1 cut(s) 323
BmeRI GACNNNNNGTC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 2 cut(s) 660, 732
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 362, 801, 830
BpuMI CCSGG 1 cut(s) 732
Bsa29I ATCGAT 2 cut(s) 182, 306
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 658
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse1I ACTGG 3 cut(s) 82, 598, 758
Bse3DI GCAATG 1 cut(s) 731
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 2 cut(s) 182, 306
BseDI CCNNGG 1 cut(s) 658
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 1 cut(s) 731
BseMII CTCAG 3 cut(s) 621, 660, 708
BseNI ACTGG 3 cut(s) 82, 598, 758
BseXI GCAGC 1 cut(s) 733
BshFI GGCC 1 cut(s) 730
BshVI ATCGAT 2 cut(s) 182, 306
BsiHKAI GWGCWC 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 731
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 113, 924
BsmBI CGTCTC 1 cut(s) 113
BsnI GGCC 1 cut(s) 730
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
BspACI CCGC 2 cut(s) 434, 779
BspANI GGCC 1 cut(s) 730
BspCNI CTCAG 3 cut(s) 622, 661, 709
BspDI ATCGAT 2 cut(s) 182, 306
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 1 cut(s) 583
BspQI GCTCTTC 1 cut(s) 150
BspTI CTTAAG 3 cut(s) 293, 470, 515
BsrDI GCAATG 1 cut(s) 731
BsrI ACTGG 3 cut(s) 82, 598, 758
BssECI CCNNGG 1 cut(s) 658
BssMI GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 422, 508
Bst6I CTCTTC 1 cut(s) 150
BstAFI CTTAAG 3 cut(s) 293, 470, 515
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 6 cut(s) 333, 630, 669, 717, 768, 921
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 7 cut(s) 156, 186, 292, 427, 578, 829, 892
BstMAI GTCTC 2 cut(s) 113, 924
BstMBI GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
BstMWI GCNNNNNNNGC 4 cut(s) 343, 727, 754, 881
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 3 cut(s) 218, 607, 761
BstSCI CCNGG 2 cut(s) 658, 730
BstV1I GCAGC 1 cut(s) 733
BstX2I RGATCY 2 cut(s) 153, 575
BstYI RGATCY 2 cut(s) 153, 575
Bsu15I ATCGAT 2 cut(s) 182, 306
BsuRI GGCC 1 cut(s) 730
BsuTUI ATCGAT 2 cut(s) 182, 306
BtgZI GCGATG 1 cut(s) 77
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 1 cut(s) 743
Cac8I GCNNGC 1 cut(s) 648
CfoI GCGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 323
ClaI ATCGAT 2 cut(s) 182, 306
CseI GACGC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 10, 850
CviAII CATG 5 cut(s) 215, 407, 530, 604, 758
CviQI GTAC 2 cut(s) 10, 850
DdeI CTNAG 6 cut(s) 333, 630, 669, 717, 768, 921
DpnI GATC 7 cut(s) 155, 185, 291, 426, 577, 828, 891
DpnII GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
DraIII CACNNNGTG 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 557
Eco57I CTGAAG 3 cut(s) 116, 582, 679
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 559
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 5 cut(s) 218, 410, 533, 607, 761
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 555, 587
FatI CATG 5 cut(s) 214, 406, 529, 603, 757
FbaI TGATCA 1 cut(s) 889
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 722, 779
FriOI GRGCYC 1 cut(s) 559
Fsp4HI GCNGC 2 cut(s) 722, 779
FspBI CTAG 2 cut(s) 20, 704
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 1 cut(s) 393
GluI GCNGC 2 cut(s) 722, 779
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 1 cut(s) 730
HapII CCGG 1 cut(s) 731
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 394
Hin1II CATG 5 cut(s) 218, 410, 533, 607, 761
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 4 cut(s) 308, 355, 439, 665
HpaII CCGG 1 cut(s) 731
HphI GGTGA 5 cut(s) 410, 463, 674, 804, 884
Hpy166II GTNNAC 3 cut(s) 55, 601, 763
Hpy188I TCNGA 6 cut(s) 32, 96, 360, 642, 670, 929
Hpy188III TCNNGA 8 cut(s) 110, 112, 149, 166, 281, 341, 560, 835
Hpy8I GTNNAC 3 cut(s) 55, 601, 763
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 4 cut(s) 19, 29, 119, 813
HpyCH4III ACNGT 2 cut(s) 422, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 343, 727, 754, 881
HpyF3I CTNAG 6 cut(s) 333, 630, 669, 717, 768, 921
Hsp92II CATG 5 cut(s) 218, 410, 533, 607, 761
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 2 cut(s) 562, 775
Lsp1109I GCAGC 1 cut(s) 733
MaeI CTAG 2 cut(s) 20, 704
MaeIII GTNAC 2 cut(s) 538, 749
MalI GATC 7 cut(s) 155, 185, 291, 426, 577, 828, 891
MboI GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
MboII GAAGA 8 cut(s) 109, 134, 137, 434, 437, 560, 685, 821
MfeI CAATTG 1 cut(s) 680
MflI RGATCY 2 cut(s) 153, 575
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 3 cut(s) 299, 680, 867
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 4 cut(s) 348, 522, 636, 825
MroXI GAANNNNTTC 3 cut(s) 129, 216, 567
MseI TTAA 5 cut(s) 294, 429, 471, 516, 788
MslI CAYNNNNRTG 2 cut(s) 405, 839
MspCI CTTAAG 3 cut(s) 293, 470, 515
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 660, 732
MunI CAATTG 1 cut(s) 680
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 4 cut(s) 343, 727, 754, 881
NciI CCSGG 1 cut(s) 732
NdeII GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
NlaIII CATG 5 cut(s) 218, 410, 533, 607, 761
NlaIV GGNNCC 1 cut(s) 777
NmuCI GTSAC 1 cut(s) 749
NsbI TGCGCA 1 cut(s) 393
NspI RCATGY 3 cut(s) 218, 607, 761
PaeR7I CTCGAG 1 cut(s) 110
PciI ACATGT 2 cut(s) 214, 603
PciSI GCTCTTC 1 cut(s) 150
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 279
PdmI GAANNNNTTC 3 cut(s) 129, 216, 567
PfeI GAWTC 4 cut(s) 308, 355, 439, 665
PkrI GCNGC 2 cut(s) 723, 780
PpuMI RGGWCCY 1 cut(s) 323
PscI ACATGT 2 cut(s) 214, 603
Psp124BI GAGCTC 1 cut(s) 559
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PsuI RGATCY 2 cut(s) 153, 575
RsaI GTAC 2 cut(s) 11, 851
RsaNI GTAC 2 cut(s) 10, 850
RseI CAYNNNNRTG 2 cut(s) 405, 839
SacI GAGCTC 1 cut(s) 559
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 5 cut(s) 294, 429, 471, 516, 788
SatI GCNGC 2 cut(s) 722, 779
Sau3AI GATC 7 cut(s) 153, 183, 289, 424, 575, 826, 889
Sau96I GGNCC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 660, 732
SduI GDGCHC 1 cut(s) 559
Sfr274I CTCGAG 1 cut(s) 110
SinI GGWCC 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 405, 839
SmlI CTYRAG 7 cut(s) 110, 293, 341, 470, 515, 816, 845
SmoI CTYRAG 7 cut(s) 110, 293, 341, 470, 515, 816, 845
Sse9I AATT 3 cut(s) 299, 680, 867
SsiI CCGC 2 cut(s) 434, 779
SspMI CTAG 2 cut(s) 20, 704
SstI GAGCTC 1 cut(s) 559
StyD4I CCNGG 2 cut(s) 658, 730
TaaI ACNGT 2 cut(s) 422, 508
TaqI TCGA 7 cut(s) 111, 182, 196, 210, 273, 282, 306
TasI AATT 3 cut(s) 299, 680, 867
TatI WGTACW 1 cut(s) 849
TauI GCSGC 1 cut(s) 781
TfiI GAWTC 4 cut(s) 308, 355, 439, 665
Tru1I TTAA 5 cut(s) 294, 429, 471, 516, 788
Tru9I TTAA 5 cut(s) 294, 429, 471, 516, 788
TscAI CASTG 1 cut(s) 750
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 1 cut(s) 721
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 3 cut(s) 209, 292, 677
TspRI CASTG 1 cut(s) 750
Vha464I CTTAAG 3 cut(s) 293, 470, 515
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 2 cut(s) 299, 867
XceI RCATGY 3 cut(s) 218, 607, 761
XhoI CTCGAG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 3 cut(s) 129, 216, 567
XspI CTAG 2 cut(s) 20, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.