AT3G59730

defense response to oomycetes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0017118
Physical Location & Seq
Reverse (-)
22064192 .. 22065957
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59730.2

Sequence Viewer

Length: 1572 bp
ATGTTTAGTGAAGTCAAAGTCTTACAAATAGTTCTTGTTCAGTGGCTTACTCTGTTTTCATTTACTTACAACTCCCATGGCACATATATCCTGGATGGATCTGCAGTTTTCAACGAAAATAGTTACTTGGTTTTAACGAACACCACGAAGCATTCCTACGGTCAAGCTTTTGACAACACAACATTTGAAATGAAGGACCAGTCTTTCTCCATCAACTTCTTCTTCGCTATTGTCCCTGAGCATAAGCAGCAAGGCTCTCACGGTATGACTTTCGCTTTTTCTCCTACAAGAGGCCTTCCCGGAGCTTCCTCCGATCAGTACCTCGGATTATTCAACAAAACAAACAACGGTAAAACTTCGAATCACGTGATAGCTATAGAGTTAGATATACATAAAGACGAAGAGTTTGAAGATATTGATGATAACCATGTTGGGATTAACATTAATGGCTTGAGATCTGTTGCCTCTGCTTCTGCTGGTTACTATGATGATAATGATGGAAGTTTCAAAAATCTTTCTTTGATCAGCGGAAAGTTAATGCGGCTTTCGATCGTTTATAGTCATCCGGATACAAAGCTCGATGTTACCTTATGCCCTGCTGAGTTTCTTGTTCCGCCACGCAAACCACTTTTGTCATTAAACCGAGATCTCTCGCAGTATGTTTTGAAACACATGCATATTGGCTTCACAGCATCTACGGGATCCATTAGAGCGCTCCATTATATGGTGCTGGTGTACACATATCCTGAAGCAGTCTATCAACCGTTGGAGTTCGGAAGAGTCCCCACCCTTCCTCCATATCCGAAGAAACCGTCTGACAGATTAAGGACGGTTTTGGCGGTCTGCTTAACGCTGGCCTTGTTTGCTGTGTTTCTCGCCTCAGGAATCGGTTTCGTCTTCTATTTGAGGCATAAGAAGGTTAAGGAGGTTCTTGAGGAATGGGAGATTCAATGTGGACCTCATAGGTTTTCTTATAAGGAGCTTTTCAATGCCACAAAGGGTTTCAAAGAGAAACAACTTCTTGGGAAAGGAGGCTTTGGTCAAGTCTACAAAGGAACACTTCCGGGTTCCGATGCAGAGATCGCTGTCAAACGGACTTCTCATGATTCAAGACAGGGGATGAGCGAGTTTCTAGCCGAGATATCGACCATTGGTCGTCTCAGACATCCAAATCTAGTCAGGCTTTTGGGATATTGTAAGCATAAAGAGAATCTCTACTTAGTTTATGATTTTATGCCTAATGGAAGCCTTGACAAGTATTTAAACCGTAGCAACACGAATGAGAATCAAGAACGGCTCACTTGGGAACAACGTTTCAAGATCATCAAAGACGTTGCATCTGCTCTACTCCACCTGCATCAAGAATGGGTTCAAGTCATCATTCATAGAGATATCAAACCGGCTAATGTTCTAATCGACCACGACATGAATGCGAGGCTCGGGGATTTCGGACTGGCGAAGCTGTATGATCAGGGATTTGATCCTCAGACATCGAGAGTTGCAGGAACATTCGGGTATATCGCACCAGAGTTTCTAAGAACAGGAAGAGCTGTTAGAGTAAAATTCTTTTAG

Protein Analysis

523

Amino Acids

59.31

Weight (kDa)

8.57

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 20 - 242 1.1e-54 Legume lectin domain
Lectin_L-type_dom PF18483 30 - 241 7e-09 Legume lectin beta-barrel domain
Pkinase PF00069 335 - 399 1.6e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 338 - 401 7.9e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 975
AarI CACCTGC 1 cut(s) 1362
Acc36I ACCTGC 1 cut(s) 1362
AccB7I CCANNNNNTGG 1 cut(s) 724
AccI GTMKAC 1 cut(s) 1047
AccIII TCCGGA 1 cut(s) 565
AciI CCGC 4 cut(s) 528, 541, 614, 839
AclI AACGTT 1 cut(s) 1312
AclWI GGATC 4 cut(s) 106, 696, 709, 1475
AcsI RAATTY 1 cut(s) 1562
AcuI CTGAAG 1 cut(s) 768
AcvI CACGTG 1 cut(s) 367
AfaI GTAC 2 cut(s) 320, 737
AfeI AGCGCT 1 cut(s) 714
AfiI CCNNNNNNNGG 2 cut(s) 290, 724
AhdI GACNNNNNGTC 1 cut(s) 1152
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 7 cut(s) 167, 305, 374, 577, 982, 1462, 1550
AluI AGCT 7 cut(s) 167, 305, 374, 577, 982, 1462, 1550
Alw26I GTCTC 1 cut(s) 1163
AlwI GGATC 4 cut(s) 106, 696, 709, 1475
Ama87I CYCGRG 1 cut(s) 1439
Aor13HI TCCGGA 1 cut(s) 565
Aor51HI AGCGCT 1 cut(s) 714
AoxI GGCC 2 cut(s) 292, 855
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 1562
ArsI GACNNNNNNTTYG 4 cut(s) 389, 421, 797, 829
AseI ATTAAT 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 1368
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 2 cut(s) 196, 956
AsuC2I CCSGG 2 cut(s) 300, 1065
AsuII TTCGAA 1 cut(s) 359
AvaI CYCGRG 1 cut(s) 1439
AvaII GGWCC 2 cut(s) 196, 956
AxyI CCTNAGG 1 cut(s) 880
BamHI GGATCC 1 cut(s) 701
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 889
BbvI GCAGC 1 cut(s) 259
BccI CCATC 3 cut(s) 89, 218, 491
BceAI ACGGC 1 cut(s) 1310
BcgI CGANNNNNNTGC 2 cut(s) 1412, 1446
BciT130I CCWGG 1 cut(s) 92
BciVI GTATCC 1 cut(s) 562
BclI TGATCA 2 cut(s) 522, 1468
BcnI CCSGG 2 cut(s) 300, 1065
BcoDI GTCTC 1 cut(s) 1163
BfaI CTAG 2 cut(s) 1133, 1175
BfmI CTRYAG 2 cut(s) 102, 375
BfoI RGCGCY 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 1362
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 2 cut(s) 455, 646
BisI GCNGC 2 cut(s) 248, 542
BlsI GCNGC 2 cut(s) 249, 543
Bme1390I CCNGG 3 cut(s) 92, 300, 1065
Bme18I GGWCC 2 cut(s) 196, 956
BmeRI GACNNNNNGTC 1 cut(s) 1152
BmeT110I CYCGRG 1 cut(s) 1439
BmgT120I GGNCC 2 cut(s) 196, 956
BmiI GGNNCC 2 cut(s) 703, 1069
BmrFI CCNGG 3 cut(s) 92, 300, 1065
BmsI GCATC 4 cut(s) 701, 1063, 1346, 1366
BpiI GAAGAC 1 cut(s) 889
Bpu10I CCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 472, 953
BpuMI CCSGG 2 cut(s) 300, 1065
BsaAI YACGTR 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 76, 322
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 724
Bse118I RCCGGY 1 cut(s) 1399
Bse1I ACTGG 2 cut(s) 199, 1458
Bse21I CCTNAGG 1 cut(s) 880
BseAI TCCGGA 1 cut(s) 565
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 76, 322
BseGI GGATG 4 cut(s) 100, 562, 1125, 1165
BseLI CCNNNNNNNGG 2 cut(s) 290, 724
BseMII CTCAG 5 cut(s) 228, 591, 894, 1174, 1499
BseNI ACTGG 2 cut(s) 199, 1458
BseXI GCAGC 1 cut(s) 259
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 2 cut(s) 294, 857
BsiEI CGRYCG 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 1439
BsiSI CCGG 4 cut(s) 300, 566, 1064, 1400
BslFI GGGAC 2 cut(s) 218, 767
BslI CCNNNNNNNGG 2 cut(s) 290, 724
BsmAI GTCTC 1 cut(s) 1163
BsmBI CGTCTC 1 cut(s) 1163
BsmFI GGGAC 2 cut(s) 218, 767
BsmI GAATGC 2 cut(s) 151, 1435
BsnI GGCC 2 cut(s) 294, 857
BsoBI CYCGRG 1 cut(s) 1439
Bsp119I TTCGAA 1 cut(s) 359
Bsp13I TCCGGA 1 cut(s) 565
Bsp1407I TGTACA 1 cut(s) 735
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 4 cut(s) 528, 541, 614, 839
BspANI GGCC 2 cut(s) 294, 857
BspCNI CTCAG 5 cut(s) 229, 592, 893, 1173, 1498
BspEI TCCGGA 1 cut(s) 565
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 2 cut(s) 703, 1069
BspMAI CTGCAG 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 1362
BspPI GGATC 4 cut(s) 106, 696, 709, 1475
BspQI GCTCTTC 1 cut(s) 1540
BspT104I TTCGAA 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 1399
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 2 cut(s) 199, 1458
BssAI RCCGGY 1 cut(s) 1399
BssECI CCNNGG 2 cut(s) 76, 322
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 7 cut(s) 161, 263, 350, 765, 813, 832, 1268
Bst6I CTCTTC 3 cut(s) 396, 772, 1540
BstAUI TGTACA 1 cut(s) 735
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 855
BstDEI CTNAG 7 cut(s) 237, 600, 880, 1160, 1219, 1485, 1535
BstDSI CCRYGG 1 cut(s) 76
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 4 cut(s) 100, 562, 1125, 1165
BstH2I RGCGCY 1 cut(s) 716
BstHHI GCGC 1 cut(s) 715
BstMAI GTCTC 1 cut(s) 1163
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 3 cut(s) 247, 863, 1082
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 3 cut(s) 90, 298, 1063
BstSFI CTRYAG 2 cut(s) 102, 375
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 889
BstX2I RGATCY 4 cut(s) 98, 455, 646, 701
BstYI RGATCY 4 cut(s) 98, 455, 646, 701
Bsu36I CCTNAGG 1 cut(s) 880
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 2 cut(s) 294, 857
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 4 cut(s) 100, 562, 1125, 1165
BtsIMutI CAGTG 1 cut(s) 47
BveI ACCTGC 1 cut(s) 1362
Cac8I GCNNGC 1 cut(s) 855
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 1399
Cfr13I GGNCC 2 cut(s) 196, 956
Csp6I GTAC 2 cut(s) 319, 736
CviAII CATG 5 cut(s) 77, 428, 673, 1103, 1426
CviQI GTAC 2 cut(s) 319, 736
DdeI CTNAG 7 cut(s) 237, 600, 880, 1160, 1219, 1485, 1535
DraI TTTAAA 1 cut(s) 1263
DriI GACNNNNNGTC 1 cut(s) 1152
Eam1104I CTCTTC 3 cut(s) 396, 772, 1540
Eam1105I GACNNNNNGTC 1 cut(s) 1152
EarI CTCTTC 3 cut(s) 396, 772, 1540
EciI GGCGGA 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 294
Eco32I GATATC 2 cut(s) 1143, 1393
Eco47I GGWCC 2 cut(s) 196, 956
Eco47III AGCGCT 1 cut(s) 714
Eco57I CTGAAG 1 cut(s) 768
Eco72I CACGTG 1 cut(s) 367
Eco81I CCTNAGG 1 cut(s) 880
Eco88I CYCGRG 1 cut(s) 1439
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 90
EcoRV GATATC 2 cut(s) 1143, 1393
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 1163
FaeI CATG 5 cut(s) 80, 431, 676, 1106, 1429
FalI AAGNNNNNCTT 2 cut(s) 1044, 1076
FaqI GGGAC 2 cut(s) 218, 767
FatI CATG 5 cut(s) 76, 427, 672, 1102, 1425
FbaI TGATCA 2 cut(s) 522, 1468
FblI GTMKAC 1 cut(s) 1047
Fnu4HI GCNGC 2 cut(s) 248, 542
FokI GGATG 4 cut(s) 107, 549, 1132, 1152
Fsp4HI GCNGC 2 cut(s) 248, 542
FspBI CTAG 2 cut(s) 1133, 1175
GlaI GCGC 1 cut(s) 714
GluI GCNGC 2 cut(s) 248, 542
HaeII RGCGCY 1 cut(s) 716
HaeIII GGCC 2 cut(s) 294, 857
HapII CCGG 4 cut(s) 300, 566, 1064, 1400
HhaI GCGC 1 cut(s) 715
Hin1II CATG 5 cut(s) 80, 431, 676, 1106, 1429
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HindIII AAGCTT 1 cut(s) 165
HinfI GANTC 7 cut(s) 361, 780, 885, 946, 1106, 1210, 1285
HpaII CCGG 4 cut(s) 300, 566, 1064, 1400
Hpy166II GTNNAC 4 cut(s) 736, 738, 956, 1048
Hpy188I TCNGA 9 cut(s) 313, 326, 776, 804, 817, 1072, 1163, 1451, 1488
Hpy8I GTNNAC 4 cut(s) 736, 738, 956, 1048
HpyAV CCTTC 4 cut(s) 187, 305, 800, 910
HpyCH4III ACNGT 7 cut(s) 161, 263, 350, 765, 813, 832, 1268
HpyCH4IV ACGT 3 cut(s) 366, 1312, 1332
HpyCH4V TGCA 6 cut(s) 104, 676, 1076, 1337, 1357, 1502
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 863, 1082
HpyF3I CTNAG 7 cut(s) 237, 600, 880, 1160, 1219, 1485, 1535
HpySE526I ACGT 3 cut(s) 366, 1312, 1332
Hsp92II CATG 5 cut(s) 80, 431, 676, 1106, 1429
HspAI GCGC 1 cut(s) 713
Kpn2I TCCGGA 1 cut(s) 565
Ksp22I TGATCA 2 cut(s) 522, 1468
LguI GCTCTTC 1 cut(s) 1540
LmnI GCTCC 3 cut(s) 302, 720, 979
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 4 cut(s) 701, 1063, 1346, 1366
MaeI CTAG 2 cut(s) 1133, 1175
MaeII ACGT 3 cut(s) 366, 1312, 1332
MaeIII GTNAC 3 cut(s) 122, 479, 583
MboII GAAGA 8 cut(s) 211, 214, 413, 422, 789, 817, 889, 1557
MflI RGATCY 4 cut(s) 98, 455, 646, 701
MluCI AATT 1 cut(s) 1562
MlyI GAGTC 1 cut(s) 789
MmeI TCCRAC 1 cut(s) 747
Mph1103I ATGCAT 1 cut(s) 678
MroI TCCGGA 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 1368
MseI TTAA 9 cut(s) 134, 438, 444, 536, 638, 824, 848, 921, 1262
MspA1I CMGCKG 1 cut(s) 528
MspI CCGG 4 cut(s) 300, 566, 1064, 1400
MspR9I CCNGG 3 cut(s) 92, 300, 1065
Mva1269I GAATGC 2 cut(s) 151, 1435
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 247, 863, 1082
NciI CCSGG 2 cut(s) 300, 1065
NcoI CCATGG 1 cut(s) 76
NlaIII CATG 5 cut(s) 80, 431, 676, 1106, 1429
NlaIV GGNNCC 2 cut(s) 703, 1069
NmeAIII GCCGAG 1 cut(s) 1162
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 676
NspV TTCGAA 1 cut(s) 359
PagI TCATGA 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 1362
PceI AGGCCT 1 cut(s) 294
PciSI GCTCTTC 1 cut(s) 1540
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PctI GAATGC 2 cut(s) 151, 1435
PdmI GAANNNNTTC 1 cut(s) 1368
PfeI GAWTC 6 cut(s) 361, 885, 946, 1106, 1210, 1285
PflMI CCANNNNNTGG 1 cut(s) 724
PfoI TCCNGGA 2 cut(s) 90, 298
PkrI GCNGC 2 cut(s) 249, 543
Ple19I CGATCG 1 cut(s) 552
PleI GAGTC 1 cut(s) 788
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 1 cut(s) 788
Ppu21I YACGTR 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 444
PsiI TTATAA 1 cut(s) 975
Psp1406I AACGTT 1 cut(s) 1312
Psp6I CCWGG 1 cut(s) 90
PspCI CACGTG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 703, 1069
PspPI GGNCC 2 cut(s) 196, 956
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 4 cut(s) 98, 455, 646, 701
PvuI CGATCG 1 cut(s) 552
RsaI GTAC 2 cut(s) 320, 737
RsaNI GTAC 2 cut(s) 319, 736
SapI GCTCTTC 1 cut(s) 1540
SaqAI TTAA 9 cut(s) 134, 438, 444, 536, 638, 824, 848, 921, 1262
SatI GCNGC 2 cut(s) 248, 542
Sau96I GGNCC 2 cut(s) 196, 956
SchI GAGTC 1 cut(s) 789
ScrFI CCNGG 3 cut(s) 92, 300, 1065
SfaNI GCATC 4 cut(s) 701, 1063, 1346, 1366
SfcI CTRYAG 2 cut(s) 102, 375
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 2 cut(s) 196, 956
SmlI CTYRAG 2 cut(s) 451, 932
SmoI CTYRAG 2 cut(s) 451, 932
Sse9I AATT 1 cut(s) 1562
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 4 cut(s) 528, 541, 614, 839
SspMI CTAG 2 cut(s) 1133, 1175
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 3 cut(s) 90, 298, 1063
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 7 cut(s) 161, 263, 350, 765, 813, 832, 1268
TaiI ACGT 3 cut(s) 369, 1315, 1335
TaqI TCGA 6 cut(s) 359, 548, 579, 1145, 1416, 1493
TasI AATT 1 cut(s) 1562
TatI WGTACW 1 cut(s) 735
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 6 cut(s) 361, 885, 946, 1106, 1210, 1285
Tru1I TTAA 9 cut(s) 134, 438, 444, 536, 638, 824, 848, 921, 1262
Tru9I TTAA 9 cut(s) 134, 438, 444, 536, 638, 824, 848, 921, 1262
TscAI CASTG 1 cut(s) 47
TseI GCWGC 1 cut(s) 247
TspDTI ATGAA 4 cut(s) 48, 206, 1373, 1442
TspGWI ACGGA 1 cut(s) 1108
TspRI CASTG 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 724
VpaK11BI GGWCC 2 cut(s) 196, 956
VspI ATTAAT 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 1 cut(s) 1562
XceI RCATGY 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 1047
XmnI GAANNNNTTC 1 cut(s) 1368
XspI CTAG 2 cut(s) 1133, 1175
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.