AT4G19410

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018295
Physical Location & Seq
Reverse (-)
10580893 .. 10585861
4969 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G19410.1

Sequence Viewer

Length: 1176 bp
ATGGGGAGGCTTAAGCAATGTTGGTCAAGTCTGCTAGTATTGGCAGTGTTGGTGATCGGAACTGGAGCCGTTCCCATCACTTATCTCCAAAGCGCCGTCGCTAAAGGAGCCGTGTGTTTGGATGGGAGTGCACCAGCTTACCATTTTGACAAAGGGTTTGGTTCGGGTGTTAACAATTGGATCGTCCACATGGAGGGAGGAGGATGGTGTACCGACGTTGCTTCATGTAATGAACGTAAGGGTACAATGAAGGGTTCTTCCAAATTCATGAACAAAGACTTTGGATTTTCCGGTATCTTGGGTGGCAAGCAAAGCACTAACCCAGATTTCTACAATTGGAACAGAATCAAAGTACGATATTGTGACGGATCATCCTTTACCGGCAATGTAGAAGCCGTTAATCCGGCAAACAAGCTGTTCTTCCGCGGTGCCCGAGTTTGGCGTGCGGTGGTTGATGATCTTATGGCTAAAGGAATGAAAAACGCTCAAAACGCTATACTCTCCGGTTGTTCAGCTGGAGCATTGGCTGCGATTCTTCATTGTGACACTTTCCGTGCCATACTTCCCCGAACAGCTAGCGTCAAATGTGTTTCTGATGCCGGTTACTTTATCCACGGTAAAGATATCACAGGAGGATCATACATTCAATCATATTACAGCAAAGTCGTCGCACTCCACGGATCTGCAAAGAGTCTTCCTGTTTCTTGTACCTCAAAAATGAAACCAGAACTCTGTTTCTTCCCGCAATACGTCGTTCCTTCCATGCGAACACCGCTCTTTGTCATCAATGCCGCTTTTGATTCTTGGCAGATCAAGAACGTTTTGGCTCCAACTGCTGTTGATAAGGGAAAAGAGTGGAAGAATTGTAAGCTTGATCTTAAGAAATGTAGTGCCGCTCAGCTCAAAACCGTCCAAGGGTTTAGAGATCAGATGATGCGAGCATTGTCACCGGTTCACAGTACACCGTCTAGAGGATTGTTCTTAGACTCGTGTCACGCTCATTGCCAAGGAGGAAGCGCCGCCTCTTGGTCCGGTGACAAAGGTCCCCAAGTCGCCAATACGAGAATTGCGAAAGCAGTCGGAAACTGGTTTTATGGCCGGAGTGCATTTCAGAAGATAGATTGTCCGTCGCCGACTTGTAATCCTACTTGTCCTGCTATCTCTACTGAAGACTAG

Protein Analysis

391

Amino Acids

42.13

Weight (kDa)

9.31

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 23 - 385 1.4e-165 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19410 AT4G19410 AT5G45280 AT5G45280
rosa_chinensis RchiOBHm_Chr7g0214961
rosa_multiflora Rmu_sc0004651.1_g000006 Rmu_ssc0000134.1_g000034
rosa_samantha Rh7AG285900 Rh7CG304900 Rh7DG289100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AccBSI CCGCTC 2 cut(s) 775, 896
AccII CGCG 1 cut(s) 426
AciI CCGC 8 cut(s) 424, 426, 446, 743, 773, 792, 894, 1020
AclI AACGTT 1 cut(s) 819
AclWI GGATC 4 cut(s) 188, 376, 643, 688
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 5 cut(s) 211, 244, 354, 709, 961
AfiI CCNNNNNNNGG 5 cut(s) 193, 438, 915, 971, 1026
AflII CTTAAG 2 cut(s) 11, 878
AgeI ACCGGT 1 cut(s) 949
AgsI TTSAA 1 cut(s) 647
AluBI AGCT 6 cut(s) 137, 415, 515, 575, 871, 901
AluI AGCT 6 cut(s) 137, 415, 515, 575, 871, 901
Alw21I GWGCWC 1 cut(s) 133
Alw44I GTGCAC 1 cut(s) 129
AlwI GGATC 4 cut(s) 188, 376, 643, 688
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 1096
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 1 cut(s) 263
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AsiGI ACCGGT 1 cut(s) 949
AspLEI GCGC 2 cut(s) 95, 1019
AspS9I GGNCC 2 cut(s) 1029, 1043
AsuHPI GGTGA 3 cut(s) 64, 939, 1046
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 2 cut(s) 1029, 1043
BaeGI GKGCMC 2 cut(s) 133, 433
BanI GGYRCC 1 cut(s) 428
BauI CACGAG 1 cut(s) 988
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 514
BccI CCATC 3 cut(s) 83, 116, 198
BceAI ACGGC 4 cut(s) 53, 80, 95, 380
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BfaI CTAG 4 cut(s) 35, 576, 969, 1174
BfoI RGCGCY 2 cut(s) 96, 1020
BfrI CTTAAG 2 cut(s) 11, 878
BisI GCNGC 4 cut(s) 528, 792, 894, 1020
BlpI GCTNAGC 1 cut(s) 897
BlsI GCNGC 4 cut(s) 529, 793, 895, 1021
Bme18I GGWCC 2 cut(s) 1029, 1043
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 2 cut(s) 1029, 1043
BmiI GGNNCC 5 cut(s) 67, 109, 430, 828, 1045
BmsI GCATC 2 cut(s) 586, 924
BmtI GCTAGC 1 cut(s) 579
BoxI GACNNNNGTC 2 cut(s) 990, 1041
BpiI GAAGAC 1 cut(s) 686
BpmI CTGGAG 2 cut(s) 84, 537
Bpu1102I GCTNAGC 1 cut(s) 897
BsaJI CCNNGG 5 cut(s) 424, 613, 676, 913, 1006
BsaWI WCCGGW 4 cut(s) 290, 503, 949, 1031
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 438, 915, 971, 1026
Bse118I RCCGGY 3 cut(s) 380, 599, 949
Bse1I ACTGG 2 cut(s) 67, 1091
Bse3DI GCAATG 3 cut(s) 23, 391, 1000
BseDI CCNNGG 5 cut(s) 424, 613, 676, 913, 1006
BseGI GGATG 3 cut(s) 127, 209, 371
BseLI CCNNNNNNNGG 5 cut(s) 193, 438, 915, 971, 1026
BseMI GCAATG 3 cut(s) 23, 391, 1000
BseMII CTCAG 1 cut(s) 911
BseNI ACTGG 2 cut(s) 67, 1091
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 2 cut(s) 133, 433
BseXI GCAGC 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 1098
BshNI GGYRCC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 949
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 8 cut(s) 291, 381, 404, 504, 600, 950, 1032, 1099
BslFI GGGAC 1 cut(s) 1029
BslI CCNNNNNNNGG 5 cut(s) 193, 438, 915, 971, 1026
BsmFI GGGAC 1 cut(s) 1029
BsnI GGCC 1 cut(s) 1098
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 133, 433
Bsp143I GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
Bsp1720I GCTNAGC 1 cut(s) 897
BspACI CCGC 8 cut(s) 424, 426, 446, 743, 773, 792, 894, 1020
BspANI GGCC 1 cut(s) 1098
BspCNI CTCAG 1 cut(s) 910
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 5 cut(s) 67, 109, 430, 828, 1045
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 4 cut(s) 188, 376, 643, 688
BspT107I GGYRCC 1 cut(s) 428
BspTI CTTAAG 2 cut(s) 11, 878
BsrBI CCGCTC 2 cut(s) 775, 896
BsrDI GCAATG 3 cut(s) 23, 391, 1000
BsrFI RCCGGY 3 cut(s) 380, 599, 949
BsrI ACTGG 2 cut(s) 67, 1091
BssAI RCCGGY 3 cut(s) 380, 599, 949
BssECI CCNNGG 5 cut(s) 424, 613, 676, 913, 1006
BssMI GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 2 cut(s) 913, 1006
Bst2BI CACGAG 1 cut(s) 988
Bst4CI ACNGT 4 cut(s) 617, 910, 959, 966
BstAFI CTTAAG 2 cut(s) 11, 878
BstAPI GCANNNNNTGC 1 cut(s) 527
BstC8I GCNNGC 4 cut(s) 308, 444, 577, 939
BstDEI CTNAG 2 cut(s) 897, 982
BstDSI CCRYGG 3 cut(s) 424, 613, 676
BstF5I GGATG 3 cut(s) 127, 209, 371
BstFNI CGCG 1 cut(s) 426
BstH2I RGCGCY 2 cut(s) 96, 1020
BstHHI GCGC 2 cut(s) 95, 1019
BstKTI GATC 9 cut(s) 57, 183, 371, 460, 638, 683, 813, 877, 928
BstMBI GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
BstMWI GCNNNNNNNGC 6 cut(s) 107, 312, 491, 527, 772, 833
BstPAI GACNNNNGTC 2 cut(s) 990, 1041
BstSLI GKGCMC 2 cut(s) 133, 433
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 1 cut(s) 1098
BtgI CCRYGG 3 cut(s) 424, 613, 676
BtsCI GGATG 3 cut(s) 127, 209, 371
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 308, 444, 577, 939
CciI TCATGA 1 cut(s) 267
CfoI GCGC 2 cut(s) 95, 1019
Cfr10I RCCGGY 3 cut(s) 380, 599, 949
Cfr13I GGNCC 2 cut(s) 1029, 1043
Cfr42I CCGCGG 1 cut(s) 427
CseI GACGC 1 cut(s) 568
Csp6I GTAC 5 cut(s) 210, 243, 353, 708, 960
CspAI ACCGGT 1 cut(s) 949
CviAII CATG 4 cut(s) 190, 225, 268, 763
CviQI GTAC 5 cut(s) 210, 243, 353, 708, 960
DdeI CTNAG 2 cut(s) 897, 982
DpnI GATC 9 cut(s) 56, 182, 370, 459, 637, 682, 812, 876, 927
DpnII GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
EaeI YGGCCR 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 913, 1006
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 1029, 1043
Eco88I CYCGRG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 1043
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 913, 1006
ErhI CCWWGG 2 cut(s) 913, 1006
FaeI CATG 4 cut(s) 193, 228, 271, 766
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 1029
FatI CATG 4 cut(s) 189, 224, 267, 762
FauI CCCGC 1 cut(s) 750
Fnu4HI GCNGC 4 cut(s) 528, 792, 894, 1020
FokI GGATG 3 cut(s) 134, 216, 358
Fsp4HI GCNGC 4 cut(s) 528, 792, 894, 1020
FspBI CTAG 4 cut(s) 35, 576, 969, 1174
GlaI GCGC 2 cut(s) 94, 1018
GluI GCNGC 4 cut(s) 528, 792, 894, 1020
GsuI CTGGAG 2 cut(s) 84, 537
HaeII RGCGCY 2 cut(s) 96, 1020
HaeIII GGCC 1 cut(s) 1098
HapII CCGG 8 cut(s) 291, 381, 404, 504, 600, 950, 1032, 1099
HgaI GACGC 1 cut(s) 568
HhaI GCGC 2 cut(s) 95, 1019
Hin1II CATG 4 cut(s) 193, 228, 271, 766
Hin6I GCGC 2 cut(s) 93, 1017
HinP1I GCGC 2 cut(s) 93, 1017
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 5 cut(s) 345, 532, 691, 800, 986
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 8 cut(s) 291, 381, 404, 504, 600, 950, 1032, 1099
HphI GGTGA 3 cut(s) 64, 939, 1046
Hpy166II GTNNAC 6 cut(s) 131, 172, 187, 210, 955, 962
Hpy188I TCNGA 5 cut(s) 59, 595, 930, 1082, 1113
Hpy188III TCNNGA 3 cut(s) 268, 814, 969
Hpy8I GTNNAC 6 cut(s) 131, 172, 187, 210, 955, 962
Hpy99I CGWCG 5 cut(s) 101, 218, 671, 755, 1132
HpyAV CCTTC 2 cut(s) 244, 768
HpyCH4III ACNGT 4 cut(s) 617, 910, 959, 966
HpyCH4IV ACGT 4 cut(s) 216, 235, 750, 819
HpyCH4V TGCA 3 cut(s) 131, 686, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 312, 491, 527, 772, 833
HpyF3I CTNAG 2 cut(s) 897, 982
HpySE526I ACGT 4 cut(s) 216, 235, 750, 819
Hsp92II CATG 4 cut(s) 193, 228, 271, 766
HspAI GCGC 2 cut(s) 93, 1017
KspAI GTTAAC 1 cut(s) 172
KspI CCGCGG 1 cut(s) 427
Kzo9I GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
LmnI GCTCC 4 cut(s) 65, 107, 518, 832
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 2 cut(s) 586, 924
MaeI CTAG 4 cut(s) 35, 576, 969, 1174
MaeII ACGT 4 cut(s) 216, 235, 750, 819
MaeIII GTNAC 6 cut(s) 362, 542, 602, 945, 992, 1034
MalI GATC 9 cut(s) 56, 182, 370, 459, 637, 682, 812, 876, 927
MbiI CCGCTC 2 cut(s) 775, 896
MboI GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
MboII GAAGA 7 cut(s) 249, 412, 527, 686, 730, 871, 1126
MfeI CAATTG 2 cut(s) 175, 334
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 2 cut(s) 133, 433
MluCI AATT 5 cut(s) 175, 263, 334, 862, 1065
MlyI GAGTC 2 cut(s) 700, 980
MmeI TCCRAC 2 cut(s) 854, 1060
MnlI CCTC 8 cut(s) 187, 191, 194, 626, 721, 965, 1004, 1033
MseI TTAA 4 cut(s) 12, 171, 399, 879
MspA1I CMGCKG 2 cut(s) 426, 515
MspCI CTTAAG 2 cut(s) 11, 878
MspI CCGG 8 cut(s) 291, 381, 404, 504, 600, 950, 1032, 1099
MunI CAATTG 2 cut(s) 175, 334
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 6 cut(s) 107, 312, 491, 527, 772, 833
NdeII GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
NheI GCTAGC 1 cut(s) 575
NlaIII CATG 4 cut(s) 193, 228, 271, 766
NlaIV GGNNCC 5 cut(s) 67, 109, 430, 828, 1045
NmuCI GTSAC 5 cut(s) 362, 542, 945, 992, 1034
PagI TCATGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 1067
PfeI GAWTC 3 cut(s) 345, 532, 800
PinAI ACCGGT 1 cut(s) 949
PkrI GCNGC 4 cut(s) 529, 793, 895, 1021
PleI GAGTC 2 cut(s) 699, 980
PpsI GAGTC 2 cut(s) 699, 980
PpuMI RGGWCCY 1 cut(s) 1043
PshAI GACNNNNGTC 2 cut(s) 990, 1041
Psp1406I AACGTT 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 1043
PspN4I GGNNCC 5 cut(s) 67, 109, 430, 828, 1045
PspPI GGNCC 2 cut(s) 1029, 1043
PspPPI RGGWCCY 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 5 cut(s) 211, 244, 354, 709, 961
RsaNI GTAC 5 cut(s) 210, 243, 353, 708, 960
SacII CCGCGG 1 cut(s) 427
SaqAI TTAA 4 cut(s) 12, 171, 399, 879
SatI GCNGC 4 cut(s) 528, 792, 894, 1020
Sau3AI GATC 9 cut(s) 54, 180, 368, 457, 635, 680, 810, 874, 925
Sau96I GGNCC 2 cut(s) 1029, 1043
SchI GAGTC 2 cut(s) 700, 980
SduI GDGCHC 2 cut(s) 133, 433
SfaNI GCATC 2 cut(s) 586, 924
Sfr303I CCGCGG 1 cut(s) 427
SgrBI CCGCGG 1 cut(s) 427
SinI GGWCC 2 cut(s) 1029, 1043
SmlI CTYRAG 2 cut(s) 11, 878
SmoI CTYRAG 2 cut(s) 11, 878
Sse9I AATT 5 cut(s) 175, 263, 334, 862, 1065
SsiI CCGC 8 cut(s) 424, 426, 446, 743, 773, 792, 894, 1020
SspMI CTAG 4 cut(s) 35, 576, 969, 1174
StyI CCWWGG 2 cut(s) 913, 1006
TaaI ACNGT 4 cut(s) 617, 910, 959, 966
TaiI ACGT 4 cut(s) 219, 238, 753, 822
TasI AATT 5 cut(s) 175, 263, 334, 862, 1065
TatI WGTACW 1 cut(s) 959
TauI GCSGC 3 cut(s) 794, 896, 1022
TfiI GAWTC 3 cut(s) 345, 532, 800
Tru1I TTAA 4 cut(s) 12, 171, 399, 879
Tru9I TTAA 4 cut(s) 12, 171, 399, 879
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 5 cut(s) 362, 542, 945, 992, 1034
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 5 cut(s) 362, 542, 945, 992, 1034
TspDTI ATGAA 8 cut(s) 213, 246, 256, 263, 284, 491, 527, 734
TspGWI ACGGA 4 cut(s) 381, 542, 693, 1116
TspRI CASTG 1 cut(s) 51
Vha464I CTTAAG 2 cut(s) 11, 878
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 2 cut(s) 1029, 1043
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 968
XspI CTAG 4 cut(s) 35, 576, 969, 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.