AT5G45280

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0018295
Physical Location & Seq
Forward (+)
18345534 .. 18349695
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45280.1

Sequence Viewer

Length: 1113 bp
ATGACGTGGCTAAAACAAATGTGGTCAAGCATTTTAGTGCTAGCCGTGGTGGTGATCGGAGCCAGAGCTGTGCCCATCACGTATCTTGAATCTGCAGTGGCTAAAGGAGCTGTGTGTTTGGATGGTAGCGCGCCAGCATACCATTTCGATAAGGGATCGGGCTCAGGAGTGAACAATTGGATCGTTCATATGGAAGGAGGAGGATGGTGCACTGATATAGCTACATGCGTTCAACGTAAAAGTACAATGAAGGGTTCTTCTAAGCTTATGAACAAAGACTTTGGTTTCTCCGGTATCTTGGGTGGCAAGCAAAGCACCAATCCAGACTTTTACAATTGGAATAGAATCAAAGTTCGGTATTGCGATGGTTCATCTTTCACCGGTGATATAGAAGCTGTTGATCCGACGCACAAGCTGTTCTTTCGCGGTGCTAGGGTTTGGCGTGCGGTGATCGATGATCTTATGGCTAAGGGAATGAGCAACGCTCAAAACGCTATACTCTCCGGTTGTTCAGCTGGAGCATTGGCTGCGATTCTACATTGCGACCAGTTCAAGTCCACTCTCCCTAAAACGGCTAAGGTCAAATGTGTTTCTGACGCTGGTTACTTCATCCACGGTAAGGATATCACTGGAGGATCTTACATCCAATCCTACTACGCCAAAGTCGTAGCGACCCACGGATCTGCAAAGAGTCTACCTGCTTCTTGCACCTCAAGTATGAAGCCTGATTTGTGTTTCTTCCCTCAATACGTTGCTAAGACCTTACAAACACCGCTTTTCGTCATCAATGCCGCCTTCGATTCTTGGCAGATCAAGAATGTATTGGCACCAACCTCTGTTGATAAAAGCAAAGCATGGAAGACTTGTAAGCTTGATCTTAAGAAGTGTACGGCCGCTCAGCTTCAAACCGTTCAAGAGACCAAGTGTTGGCTGCGTTGGCGCCTGTTCGATCCGCGACGACGAACGGATTGTTCTTGGACTCGTGCCATGCTCATTGCCAAGGTGGAAGCGCTGCCACTTGGTCCGGCGACAAAGGTCCCACAGTCGCGAATACGAAAATGGCTAAGGCGGTTGGAGATTGGTTCTTTGAGAGGAGTACGTTTCAGAACGTAG

Protein Analysis

370

Amino Acids

40.79

Weight (kDa)

9.73

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 23 - 311 4e-136 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19410 AT4G19410 AT5G45280 AT5G45280
rosa_chinensis RchiOBHm_Chr7g0214961
rosa_multiflora Rmu_sc0004651.1_g000006 Rmu_ssc0000134.1_g000034
rosa_samantha Rh7AG285900 Rh7CG304900 Rh7DG289100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 706
AccB1I GGYRCC 2 cut(s) 826, 939
AccB7I CCANNNNNTGG 1 cut(s) 927
AccBSI CCGCTC 1 cut(s) 896
AccI GTMKAC 1 cut(s) 694
AccII CGCG 4 cut(s) 131, 426, 955, 1048
AciI CCGC 7 cut(s) 426, 446, 773, 792, 894, 953, 1069
AclWI GGATC 6 cut(s) 163, 188, 395, 643, 688, 944
AcoI YGGCCR 1 cut(s) 891
AcyI GRCGYC 1 cut(s) 940
AfaI GTAC 3 cut(s) 244, 889, 1098
AfeI AGCGCT 1 cut(s) 1011
AfiI CCNNNNNNNGG 3 cut(s) 571, 619, 927
AflII CTTAAG 1 cut(s) 878
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 5 cut(s) 89, 233, 553, 905, 914
AjiI CACGTC 1 cut(s) 6
AluBI AGCT 9 cut(s) 68, 110, 221, 265, 395, 415, 515, 871, 901
AluI AGCT 9 cut(s) 68, 110, 221, 265, 395, 415, 515, 871, 901
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 911
Alw44I GTGCAC 1 cut(s) 208
AlwI GGATC 6 cut(s) 163, 188, 395, 643, 688, 944
Aor51HI AGCGCT 1 cut(s) 1011
AoxI GGCC 1 cut(s) 891
ApaLI GTGCAC 1 cut(s) 208
ApeKI GCWGC 3 cut(s) 527, 931, 1012
AsiGI ACCGGT 1 cut(s) 380
AspLEI GCGC 4 cut(s) 131, 133, 942, 1012
AspS9I GGNCC 2 cut(s) 1022, 1036
AsuHPI GGTGA 4 cut(s) 64, 370, 395, 460
AsuNHI GCTAGC 1 cut(s) 40
AvaII GGWCC 2 cut(s) 1022, 1036
BaeGI GKGCMC 2 cut(s) 75, 212
BanI GGYRCC 2 cut(s) 826, 939
BanII GRGCYC 1 cut(s) 164
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 514, 918, 999
BccI CCATC 4 cut(s) 83, 116, 198, 359
BceAI ACGGC 3 cut(s) 29, 588, 906
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 2 cut(s) 41, 432
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 2 cut(s) 943, 1013
BfrI CTTAAG 1 cut(s) 878
BfuAI ACCTGC 1 cut(s) 706
BisI GCNGC 5 cut(s) 528, 792, 894, 932, 1013
BlpI GCTNAGC 1 cut(s) 897
BlsI GCNGC 5 cut(s) 529, 793, 895, 933, 1014
Bme18I GGWCC 2 cut(s) 1022, 1036
BmgBI CACGTC 1 cut(s) 6
BmgT120I GGNCC 2 cut(s) 1022, 1036
BmiI GGNNCC 4 cut(s) 61, 828, 941, 1038
BmtI GCTAGC 1 cut(s) 44
BoxI GACNNNNGTC 1 cut(s) 1034
BpiI GAAGAC 1 cut(s) 866
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpmI CTGGAG 2 cut(s) 537, 651
Bpu10I CCTNAGC 4 cut(s) 163, 468, 576, 1064
Bpu1102I GCTNAGC 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 697
Bsa29I ATCGAT 1 cut(s) 453
BsaAI YACGTR 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 940
BsaI GGTCTC 1 cut(s) 911
BsaJI CCNNGG 4 cut(s) 45, 613, 676, 999
BsaWI WCCGGW 3 cut(s) 290, 380, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 571, 619, 927
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 2 cut(s) 547, 634
Bse3DI GCAATG 2 cut(s) 538, 993
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 4 cut(s) 45, 613, 676, 999
BseGI GGATG 4 cut(s) 127, 209, 609, 642
BseLI CCNNNNNNNGG 3 cut(s) 571, 619, 927
BseMI GCAATG 2 cut(s) 538, 993
BseMII CTCAG 2 cut(s) 177, 911
BseNI ACTGG 2 cut(s) 547, 634
BsePI GCGCGC 1 cut(s) 129
BseRI GAGGAG 2 cut(s) 213, 1107
BseSI GKGCMC 2 cut(s) 75, 212
BseX3I CGGCCG 1 cut(s) 891
BseXI GCAGC 3 cut(s) 514, 918, 999
Bsh1236I CGCG 4 cut(s) 131, 426, 955, 1048
Bsh1285I CGRYCG 1 cut(s) 894
BshFI GGCC 1 cut(s) 893
BshNI GGYRCC 2 cut(s) 826, 939
BshTI ACCGGT 1 cut(s) 380
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 894
BsiHKAI GWGCWC 1 cut(s) 212
BsiSI CCGG 4 cut(s) 291, 381, 504, 1025
BslFI GGGAC 1 cut(s) 1022
BslI CCNNNNNNNGG 3 cut(s) 571, 619, 927
BsmAI GTCTC 1 cut(s) 911
BsmFI GGGAC 1 cut(s) 1022
BsnI GGCC 1 cut(s) 893
Bso31I GGTCTC 1 cut(s) 911
Bsp1286I GDGCHC 3 cut(s) 75, 164, 212
Bsp1720I GCTNAGC 1 cut(s) 897
Bsp68I TCGCGA 1 cut(s) 1048
BspACI CCGC 7 cut(s) 426, 446, 773, 792, 894, 953, 1069
BspANI GGCC 1 cut(s) 893
BspCNI CTCAG 2 cut(s) 176, 910
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 4 cut(s) 131, 426, 955, 1048
BspLI GGNNCC 4 cut(s) 61, 828, 941, 1038
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 706
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 6 cut(s) 163, 188, 395, 643, 688, 944
BspT107I GGYRCC 2 cut(s) 826, 939
BspTI CTTAAG 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 538, 993
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 2 cut(s) 547, 634
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 4 cut(s) 45, 613, 676, 999
BssHII GCGCGC 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 940
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 1 cut(s) 999
Bst2BI CACGAG 1 cut(s) 981
Bst4CI ACNGT 3 cut(s) 617, 910, 1044
BstACI GRCGYC 1 cut(s) 940
BstAFI CTTAAG 1 cut(s) 878
BstAPI GCANNNNNTGC 1 cut(s) 527
BstBAI YACGTR 1 cut(s) 81
BstC8I GCNNGC 5 cut(s) 42, 131, 135, 308, 444
BstDEI CTNAG 7 cut(s) 163, 261, 468, 576, 756, 897, 1064
BstDSI CCRYGG 3 cut(s) 45, 613, 676
BstF5I GGATG 4 cut(s) 127, 209, 609, 642
BstFNI CGCG 4 cut(s) 131, 426, 955, 1048
BstH2I RGCGCY 2 cut(s) 943, 1013
BstHHI GCGC 4 cut(s) 131, 133, 942, 1012
BstMAI GTCTC 1 cut(s) 911
BstMCI CGRYCG 1 cut(s) 894
BstMWI GCNNNNNNNGC 5 cut(s) 107, 312, 491, 527, 937
BstNSI RCATGY 1 cut(s) 228
BstPAI GACNNNNGTC 1 cut(s) 1034
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 2 cut(s) 75, 212
BstUI CGCG 4 cut(s) 131, 426, 955, 1048
BstV1I GCAGC 3 cut(s) 514, 918, 999
BstV2I GAAGAC 1 cut(s) 866
BstX2I RGATCY 2 cut(s) 635, 680
BstYI RGATCY 2 cut(s) 635, 680
BstZI CGGCCG 1 cut(s) 891
Bsu15I ATCGAT 1 cut(s) 453
BsuRI GGCC 1 cut(s) 893
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 3 cut(s) 45, 613, 676
BtgZI GCGATG 1 cut(s) 378
BtrI CACGTC 1 cut(s) 6
BtsCI GGATG 4 cut(s) 127, 209, 609, 642
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 102, 210, 627
BtuMI TCGCGA 1 cut(s) 1048
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 5 cut(s) 42, 131, 135, 308, 444
CfoI GCGC 4 cut(s) 131, 133, 942, 1012
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 1022, 1036
ClaI ATCGAT 1 cut(s) 453
CseI GACGC 2 cut(s) 415, 605
Csp6I GTAC 3 cut(s) 243, 888, 1097
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 3 cut(s) 225, 855, 988
CviQI GTAC 3 cut(s) 243, 888, 1097
DdeI CTNAG 7 cut(s) 163, 261, 468, 576, 756, 897, 1064
DinI GGCGCC 1 cut(s) 941
EaeI YGGCCR 1 cut(s) 891
EagI CGGCCG 1 cut(s) 891
EclXI CGGCCG 1 cut(s) 891
Eco130I CCWWGG 1 cut(s) 999
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 911
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 1022, 1036
Eco47III AGCGCT 1 cut(s) 1011
Eco52I CGGCCG 1 cut(s) 891
EcoO109I RGGNCCY 1 cut(s) 1036
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 999
EcoT38I GRGCYC 1 cut(s) 164
EgeI GGCGCC 1 cut(s) 941
EheI GGCGCC 1 cut(s) 941
ErhI CCWWGG 1 cut(s) 999
FaeI CATG 3 cut(s) 228, 858, 991
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 1022
FatI CATG 3 cut(s) 224, 854, 987
FauNDI CATATG 1 cut(s) 189
FblI GTMKAC 1 cut(s) 694
Fnu4HI GCNGC 5 cut(s) 528, 792, 894, 932, 1013
FokI GGATG 4 cut(s) 134, 216, 596, 629
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 5 cut(s) 528, 792, 894, 932, 1013
FspBI CTAG 2 cut(s) 41, 432
GlaI GCGC 4 cut(s) 130, 132, 941, 1011
GluI GCNGC 5 cut(s) 528, 792, 894, 932, 1013
GsuI CTGGAG 2 cut(s) 537, 651
HaeII RGCGCY 2 cut(s) 943, 1013
HaeIII GGCC 1 cut(s) 893
HapII CCGG 4 cut(s) 291, 381, 504, 1025
HgaI GACGC 2 cut(s) 415, 605
HhaI GCGC 4 cut(s) 131, 133, 942, 1012
Hin1I GRCGYC 1 cut(s) 940
Hin1II CATG 3 cut(s) 228, 858, 991
Hin6I GCGC 4 cut(s) 129, 131, 940, 1010
HinP1I GCGC 4 cut(s) 129, 131, 940, 1010
HindIII AAGCTT 2 cut(s) 263, 869
HinfI GANTC 6 cut(s) 89, 345, 532, 691, 800, 979
HpaII CCGG 4 cut(s) 291, 381, 504, 1025
HphI GGTGA 4 cut(s) 64, 370, 395, 460
Hpy166II GTNNAC 5 cut(s) 172, 210, 558, 695, 888
Hpy188I TCNGA 4 cut(s) 59, 405, 595, 1106
Hpy188III TCNNGA 6 cut(s) 86, 165, 323, 814, 914, 1047
Hpy8I GTNNAC 5 cut(s) 172, 210, 558, 695, 888
Hpy99I CGWCG 3 cut(s) 409, 960, 963
HpyAV CCTTC 3 cut(s) 188, 244, 805
HpyCH4III ACNGT 3 cut(s) 617, 910, 1044
HpyCH4IV ACGT 6 cut(s) 5, 80, 235, 750, 1099, 1109
HpyCH4V TGCA 4 cut(s) 95, 210, 686, 708
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 312, 491, 527, 937
HpyF3I CTNAG 7 cut(s) 163, 261, 468, 576, 756, 897, 1064
HpySE526I ACGT 6 cut(s) 5, 80, 235, 750, 1099, 1109
Hsp92I GRCGYC 1 cut(s) 940
Hsp92II CATG 3 cut(s) 228, 858, 991
HspAI GCGC 4 cut(s) 129, 131, 940, 1010
KasI GGCGCC 1 cut(s) 939
LmnI GCTCC 3 cut(s) 59, 107, 518
Lsp1109I GCAGC 3 cut(s) 514, 918, 999
MaeI CTAG 2 cut(s) 41, 432
MaeII ACGT 6 cut(s) 5, 80, 235, 750, 1099, 1109
MaeIII GTNAC 1 cut(s) 602
MbiI CCGCTC 1 cut(s) 896
MboII GAAGA 3 cut(s) 249, 730, 871
MfeI CAATTG 2 cut(s) 175, 334
MflI RGATCY 2 cut(s) 635, 680
MhlI GDGCHC 3 cut(s) 75, 164, 212
MluCI AATT 2 cut(s) 175, 334
Mly113I GGCGCC 1 cut(s) 940
MlyI GAGTC 2 cut(s) 700, 973
MmeI TCCRAC 2 cut(s) 428, 1053
MnlI CCTC 7 cut(s) 191, 194, 626, 721, 753, 844, 1085
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 4 cut(s) 291, 381, 504, 1025
MunI CAATTG 2 cut(s) 175, 334
MvnI CGCG 4 cut(s) 131, 426, 955, 1048
MwoI GCNNNNNNNGC 5 cut(s) 107, 312, 491, 527, 937
NarI GGCGCC 1 cut(s) 940
NdeI CATATG 1 cut(s) 189
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 228, 858, 991
NlaIV GGNNCC 4 cut(s) 61, 828, 941, 1038
NruI TCGCGA 1 cut(s) 1048
NspI RCATGY 1 cut(s) 228
PauI GCGCGC 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PfeI GAWTC 4 cut(s) 89, 345, 532, 800
PflMI CCANNNNNTGG 1 cut(s) 927
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 5 cut(s) 529, 793, 895, 933, 1014
PleI GAGTC 2 cut(s) 699, 973
PluTI GGCGCC 1 cut(s) 943
PpsI GAGTC 2 cut(s) 699, 973
Ppu21I YACGTR 1 cut(s) 81
PpuMI RGGWCCY 1 cut(s) 1036
PshAI GACNNNNGTC 1 cut(s) 1034
Psp5II RGGWCCY 1 cut(s) 1036
PspN4I GGNNCC 4 cut(s) 61, 828, 941, 1038
PspPI GGNCC 2 cut(s) 1022, 1036
PspPPI RGGWCCY 1 cut(s) 1036
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 2 cut(s) 635, 680
PteI GCGCGC 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 515
RruI TCGCGA 1 cut(s) 1048
RsaI GTAC 3 cut(s) 244, 889, 1098
RsaNI GTAC 3 cut(s) 243, 888, 1097
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 5 cut(s) 528, 792, 894, 932, 1013
Sau96I GGNCC 2 cut(s) 1022, 1036
SchI GAGTC 2 cut(s) 700, 973
SduI GDGCHC 3 cut(s) 75, 164, 212
SfcI CTRYAG 1 cut(s) 93
SfoI GGCGCC 1 cut(s) 941
SgrAI CRCCGGYG 1 cut(s) 380
SinI GGWCC 2 cut(s) 1022, 1036
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 712, 878
SmoI CTYRAG 2 cut(s) 712, 878
Sse9I AATT 2 cut(s) 175, 334
SsiI CCGC 7 cut(s) 426, 446, 773, 792, 894, 953, 1069
SspDI GGCGCC 1 cut(s) 939
SspMI CTAG 2 cut(s) 41, 432
StyI CCWWGG 1 cut(s) 999
TaaI ACNGT 3 cut(s) 617, 910, 1044
TaiI ACGT 6 cut(s) 8, 83, 238, 753, 1102, 1112
TaqI TCGA 4 cut(s) 147, 453, 798, 948
TasI AATT 2 cut(s) 175, 334
TatI WGTACW 1 cut(s) 242
TauI GCSGC 2 cut(s) 794, 896
TfiI GAWTC 4 cut(s) 89, 345, 532, 800
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 3 cut(s) 102, 217, 634
TseI GCWGC 3 cut(s) 527, 931, 1012
TspDTI ATGAA 6 cut(s) 176, 263, 284, 360, 598, 734
TspGWI ACGGA 2 cut(s) 693, 980
TspRI CASTG 3 cut(s) 102, 217, 634
Van91I CCANNNNNTGG 1 cut(s) 927
Vha464I CTTAAG 1 cut(s) 878
VneI GTGCAC 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 1022, 1036
XceI RCATGY 1 cut(s) 228
XmiI GTMKAC 1 cut(s) 694
XspI CTAG 2 cut(s) 41, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.