AT4G19860

Lecithin:cholesterol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016182
Physical Location & Seq
Reverse (-)
10777512 .. 10780470
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G19860.1

Sequence Viewer

Length: 1608 bp
ATGTCTCTATTACTGGAAGAGATCATTAGATCAGTAGAGGCTTTGCTGAAGCTCAGAAATCGGAATCAGGAACCCTATGTTGACCCGAATCTAAACCCGGTTCTTCTAGTTCCAGGAATCGCTGGATCAATTCTAAACGCCGTTGATCATGAGAACGGGAACGAAGAACGTGTTTGGGTTAGGATCTTTGGTGCTGATCATGAGTTTCGAACAAAGATGTGGTCTCGATTTGATCCTTCAACTGGTAAAACGATATCTCTTGATCCAAAAACGAGTATTGTTGTTCCTCAAGACAGAGCTGGGCTACATGCAATTGATGTCTTAGACCCTGATATGATTGTTGGCCGTGAGTCTGTGTACTATTTCCATGAGATGATTGTTGAGATGATCGGATGGGGATTTGAAGAAGGGAAAACCCTTTTTGGTTTTGGTTATGATTTCCGCCAAAGCAACAGACTGCAGGAAACGTTGGACCAGTTTGCTAAAAAGTTGGAAACTGTTTATAAAGCCTCAGGAGAGAAGAAGATTAATGTTATTAGTCATTCTATGGGAGGACTATTGGTGAAATGTTTCATGGGTCTGCATAGTGATATATTCGAGAAGTATGTACAGAATTGGATTGCTATTGCTGCTCCATTTCGAGGTGCTCCTGGATATATCACATCGACTTTATTGAATGGAATGTCGTTTGTCAATGGTTGGGAACAGAACTTTTTCGTCTCTAAGTGGAGCATGCATCAGCTGCTTATTGAGTGTCCATCCATATATGAGCTGATGTGTTGTCCGTATTTTAAATGGGAGCTCCCTCCCGTCTTAGAGCTGTGGAGAGAGAAAGAGAGCAATGATGGAGTTGGAACCTCTTATGTTGTTCTTGAGTCTTACTGTAGCCTGGAGAGCCTTGAAGTTTTTACGAAATCTCTTTCGAATAATACAGCTGATTATTGTGGAGAGTCGATCGATCTTCCTTTTAACTGGAAGATCATGGAGTGGGCTCACAAAACCAAGCAAGTATTAGCCTCTGCCAAGCTGCCTCCGAAAGTTAAATTCTATAACATATATGGGACCAATCTAGAAACCCCTCATAGTGTTTGCTATGGGAATGAGAAGATGCCCGTTAAAGATCTAACGAATCTAAGATACTTCCAGCCGACATATATATGCGTGGATGGTGATGGCACAGTCCCGATGGAATCTGCCATGGCGGATGGGCTTGAAGCAGTAGCAAGAGTTGGAGTCCCTGGTGAGCACCGAGGAATCCTCAACGATCACCGTGTCTTCCGAATGCTCAAAAAATGGCTAAATGTAGGCGAACCAGACCCGTTCTACAACCCAGTAAACGATTATGTCATCCTTCCCACCACATATGAATTTGAGAAATTCCATGAGAATGGACTCGAGGTTGCTTCCGTGAAAGAATCGTGGGACATCATATCAGATGACAACAATATCGGCACAACCGGGTCAACCGTGAACTCCATATCAGTCTCTCAACCTGGAGATGATCAAAACCCTCAAGCTGAAGCTCGTGCAACCTTAACCGTCCAACCACAAAGCGATGGTAGACAACATGTAGAGCTCAATGCTGTAAGTGTCTCTGTTGATGCATAA

Protein Analysis

535

Amino Acids

60.43

Weight (kDa)

5.04

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 99 - 399 4e-28 Lecithin:cholesterol acyltransferase
DUF676 PF05057 164 - 238 8.3e-06 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19860
fragaria_vesca FvH4_3g12200
malus_domestica MD05G1252800.v1.1 MD10G1233900.v1.1
prunus_persica Prupe.4G111100_v2.0.a1
pyrus_communis pycom05g23100 pycom10g19620
rosa_chinensis RchiOBHm_Chr5g0020001
rosa_laevigata RLG00000032492
rosa_multiflora Rmu_sc0002250.1_g000005
rosa_roxburghii Rroxscaffold_1G00058190
rosa_rugosa Rorug05G0056100
rosa_samantha Rh5AG146300 Rh5BG145800
rosa_wichuraiana Rw5G012920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AccI GTMKAC 1 cut(s) 1561
AciI CCGC 2 cut(s) 442, 1202
AclI AACGTT 1 cut(s) 467
AclWI GGATC 4 cut(s) 133, 191, 227, 257
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 3 cut(s) 1043, 1367, 1376
AcuI CTGAAG 2 cut(s) 68, 1539
AfaI GTAC 2 cut(s) 359, 609
AfiI CCNNNNNNNGG 2 cut(s) 242, 641
AflIII ACRYGT 2 cut(s) 169, 1567
AgsI TTSAA 5 cut(s) 240, 404, 676, 902, 1214
AjnI CCWGG 5 cut(s) 112, 649, 888, 1237, 1492
Alw21I GWGCWC 4 cut(s) 649, 804, 1248, 1578
Alw26I GTCTC 5 cut(s) 9, 228, 724, 1489, 1597
AlwI GGATC 4 cut(s) 133, 191, 227, 257
Ama87I CYCGRG 1 cut(s) 1394
AoxI GGCC 1 cut(s) 343
ApeKI GCWGC 3 cut(s) 629, 742, 1027
ApoI RAATTY 3 cut(s) 1043, 1367, 1376
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AseI ATTAAT 1 cut(s) 528
Asp700I GAANNNNTTC 2 cut(s) 569, 713
AspS9I GGNCC 2 cut(s) 472, 1062
AsuC2I CCSGG 2 cut(s) 98, 1459
AsuHPI GGTGA 4 cut(s) 574, 1181, 1253, 1259
AsuII TTCGAA 2 cut(s) 208, 923
AvaI CYCGRG 1 cut(s) 1394
AvaII GGWCC 2 cut(s) 472, 1062
AxyI CCTNAGG 1 cut(s) 511
BanII GRGCYC 3 cut(s) 804, 994, 1578
BauI CACGAG 1 cut(s) 1524
BbsI GAAGAC 1 cut(s) 1267
Bbv12I GWGCWC 4 cut(s) 649, 804, 1248, 1578
BbvI GCAGC 3 cut(s) 616, 729, 1014
BccI CCATC 8 cut(s) 387, 766, 839, 1160, 1166, 1180, 1199, 1550
BceAI ACGGC 2 cut(s) 125, 330
BciT130I CCWGG 5 cut(s) 114, 651, 890, 1239, 1494
BclI TGATCA 3 cut(s) 145, 196, 1501
BcnI CCSGG 2 cut(s) 98, 1459
BcoDI GTCTC 5 cut(s) 9, 228, 724, 1489, 1597
BfaI CTAG 2 cut(s) 107, 1070
BfmI CTRYAG 2 cut(s) 458, 883
BglII AGATCT 1 cut(s) 1120
BisI GCNGC 3 cut(s) 630, 743, 1028
BlsI GCNGC 3 cut(s) 631, 744, 1029
Bme1390I CCNGG 7 cut(s) 98, 114, 651, 890, 1239, 1459, 1494
Bme18I GGWCC 2 cut(s) 472, 1062
BmeT110I CYCGRG 1 cut(s) 1394
BmgT120I GGNCC 2 cut(s) 472, 1062
BmiI GGNNCC 3 cut(s) 72, 856, 1063
BmrFI CCNGG 7 cut(s) 98, 114, 651, 890, 1239, 1459, 1494
BmrI ACTGGG 1 cut(s) 1325
BmsI GCATC 3 cut(s) 745, 1098, 1591
BmuI ACTGGG 1 cut(s) 1325
BpiI GAAGAC 1 cut(s) 1267
BplI GAGNNNNNCTC 2 cut(s) 1242, 1274
BpmI CTGGAG 2 cut(s) 911, 1515
Bpu14I TTCGAA 2 cut(s) 208, 923
BpuEI CTTGAG 3 cut(s) 273, 893, 1497
BpuMI CCSGG 2 cut(s) 98, 1459
Bsa29I ATCGAT 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 3 cut(s) 1197, 1237, 1249
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 641
Bse1I ACTGG 5 cut(s) 18, 247, 475, 977, 1331
Bse21I CCTNAGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 847
BseBI CCWGG 5 cut(s) 114, 651, 890, 1239, 1494
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 3 cut(s) 1197, 1237, 1249
BseGI GGATG 5 cut(s) 398, 758, 1171, 1210, 1347
BseLI CCNNNNNNNGG 2 cut(s) 242, 641
BseMI GCAATG 1 cut(s) 847
BseMII CTCAG 2 cut(s) 67, 525
BseNI ACTGG 5 cut(s) 18, 247, 475, 977, 1331
BseXI GCAGC 3 cut(s) 616, 729, 1014
BseYI CCCAGC 1 cut(s) 299
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 1 cut(s) 345
BshVI ATCGAT 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 4 cut(s) 649, 804, 1248, 1578
BsiHKCI CYCGRG 1 cut(s) 1394
BsiSI CCGG 2 cut(s) 98, 1458
BslFI GGGAC 4 cut(s) 1075, 1166, 1220, 1436
BslI CCNNNNNNNGG 2 cut(s) 242, 641
BsmAI GTCTC 5 cut(s) 9, 228, 724, 1489, 1597
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 4 cut(s) 1075, 1166, 1220, 1436
BsmI GAATGC 1 cut(s) 1287
BsnI GGCC 1 cut(s) 345
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 1394
Bsp119I TTCGAA 2 cut(s) 208, 923
Bsp1286I GDGCHC 5 cut(s) 649, 804, 994, 1248, 1578
Bsp1407I TGTACA 1 cut(s) 607
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 2 cut(s) 442, 1202
BspANI GGCC 1 cut(s) 345
BspCNI CTCAG 2 cut(s) 66, 524
BspDI ATCGAT 1 cut(s) 957
BspHI TCATGA 2 cut(s) 148, 199
BspLI GGNNCC 3 cut(s) 72, 856, 1063
BspMAI CTGCAG 1 cut(s) 462
BspPI GGATC 4 cut(s) 133, 191, 227, 257
BspT104I TTCGAA 2 cut(s) 208, 923
BspTNI GGTCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 847
BsrGI TGTACA 1 cut(s) 607
BsrI ACTGG 5 cut(s) 18, 247, 475, 977, 1331
BssECI CCNNGG 3 cut(s) 1197, 1237, 1249
BssSI CACGAG 1 cut(s) 1524
BssT1I CCWWGG 1 cut(s) 1197
Bst2BI CACGAG 1 cut(s) 1524
Bst2UI CCWGG 5 cut(s) 114, 651, 890, 1239, 1494
Bst4CI ACNGT 6 cut(s) 499, 884, 1180, 1271, 1468, 1540
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 742
BstAUI TGTACA 1 cut(s) 607
BstBI TTCGAA 2 cut(s) 208, 923
BstC8I GCNNGC 1 cut(s) 734
BstDEI CTNAG 6 cut(s) 53, 322, 511, 723, 814, 1133
BstDSI CCRYGG 1 cut(s) 1197
BstF5I GGATG 5 cut(s) 398, 758, 1171, 1210, 1347
BstMAI GTCTC 5 cut(s) 9, 228, 724, 1489, 1597
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 3 cut(s) 629, 742, 894
BstNI CCWGG 5 cut(s) 114, 651, 890, 1239, 1494
BstNSI RCATGY 3 cut(s) 311, 736, 1571
BstSCI CCNGG 7 cut(s) 96, 112, 649, 888, 1237, 1457, 1492
BstSFI CTRYAG 2 cut(s) 458, 883
BstV1I GCAGC 3 cut(s) 616, 729, 1014
BstV2I GAAGAC 1 cut(s) 1267
BstX2I RGATCY 2 cut(s) 183, 1120
BstXI CCANNNNNNTGG 1 cut(s) 1388
BstYI RGATCY 2 cut(s) 183, 1120
Bsu15I ATCGAT 1 cut(s) 957
Bsu36I CCTNAGG 1 cut(s) 511
BsuRI GGCC 1 cut(s) 345
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 1197
BtgZI GCGATG 1 cut(s) 1569
BtsCI GGATG 5 cut(s) 398, 758, 1171, 1210, 1347
Cac8I GCNNGC 1 cut(s) 734
CciI TCATGA 2 cut(s) 148, 199
Cfr13I GGNCC 2 cut(s) 472, 1062
ClaI ATCGAT 1 cut(s) 957
Csp6I GTAC 2 cut(s) 358, 608
CviQI GTAC 2 cut(s) 358, 608
DdeI CTNAG 6 cut(s) 53, 322, 511, 723, 814, 1133
DraI TTTAAA 1 cut(s) 793
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 2 cut(s) 431, 1217
Ecl136II GAGCTC 2 cut(s) 802, 1576
Eco130I CCWWGG 1 cut(s) 1197
Eco24I GRGCYC 3 cut(s) 804, 994, 1578
Eco31I GGTCTC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 472, 1062
Eco53kI GAGCTC 2 cut(s) 802, 1576
Eco57I CTGAAG 2 cut(s) 68, 1539
Eco81I CCTNAGG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 1394
EcoICRI GAGCTC 2 cut(s) 802, 1576
EcoRII CCWGG 5 cut(s) 112, 649, 888, 1237, 1492
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 1197
EcoT22I ATGCAT 2 cut(s) 738, 1606
EcoT38I GRGCYC 3 cut(s) 804, 994, 1578
ErhI CCWWGG 1 cut(s) 1197
Esp3I CGTCTC 1 cut(s) 724
FaqI GGGAC 4 cut(s) 1075, 1166, 1220, 1436
FauNDI CATATG 1 cut(s) 1363
FbaI TGATCA 3 cut(s) 145, 196, 1501
FblI GTMKAC 1 cut(s) 1561
Fnu4HI GCNGC 3 cut(s) 630, 743, 1028
FokI GGATG 5 cut(s) 405, 745, 1178, 1217, 1334
FriOI GRGCYC 3 cut(s) 804, 994, 1578
Fsp4HI GCNGC 3 cut(s) 630, 743, 1028
FspBI CTAG 2 cut(s) 107, 1070
GluI GCNGC 3 cut(s) 630, 743, 1028
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 2 cut(s) 911, 1515
HaeIII GGCC 1 cut(s) 345
HapII CCGG 2 cut(s) 98, 1458
HincII GTYRAC 2 cut(s) 82, 1464
HindII GTYRAC 2 cut(s) 82, 1464
HpaII CCGG 2 cut(s) 98, 1458
HphI GGTGA 4 cut(s) 574, 1181, 1253, 1259
Hpy166II GTNNAC 6 cut(s) 82, 358, 1336, 1464, 1471, 1562
Hpy188I TCNGA 6 cut(s) 56, 63, 392, 1035, 1280, 1435
Hpy8I GTNNAC 6 cut(s) 82, 358, 1336, 1464, 1471, 1562
HpyAV CCTTC 3 cut(s) 246, 401, 1361
HpyCH4III ACNGT 6 cut(s) 499, 884, 1180, 1271, 1468, 1540
HpyCH4IV ACGT 2 cut(s) 169, 467
HpyCH4V TGCA 6 cut(s) 311, 460, 583, 736, 1529, 1604
HpyF10VI GCNNNNNNNGC 3 cut(s) 629, 742, 894
HpyF3I CTNAG 6 cut(s) 53, 322, 511, 723, 814, 1133
HpySE526I ACGT 2 cut(s) 169, 467
Ksp22I TGATCA 3 cut(s) 145, 196, 1501
LmnI GCTCC 5 cut(s) 637, 652, 729, 799, 807
Lsp1109I GCAGC 3 cut(s) 616, 729, 1014
LweI GCATC 3 cut(s) 745, 1098, 1591
MaeI CTAG 2 cut(s) 107, 1070
MaeII ACGT 2 cut(s) 169, 467
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 2 cut(s) 183, 1120
MhlI GDGCHC 5 cut(s) 649, 804, 994, 1248, 1578
MluCI AATT 6 cut(s) 129, 312, 613, 1043, 1367, 1376
MlyI GAGTC 5 cut(s) 359, 884, 959, 1242, 1386
MmeI TCCRAC 5 cut(s) 450, 471, 832, 1210, 1567
Mph1103I ATGCAT 2 cut(s) 738, 1606
MroXI GAANNNNTTC 2 cut(s) 569, 713
MseI TTAA 6 cut(s) 528, 792, 969, 1041, 1116, 1535
MslI CAYNNNNRTG 2 cut(s) 1156, 1386
MspA1I CMGCKG 2 cut(s) 742, 935
MspI CCGG 2 cut(s) 98, 1458
MspR9I CCNGG 7 cut(s) 98, 114, 651, 890, 1239, 1459, 1494
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 1287
MvaI CCWGG 5 cut(s) 114, 651, 890, 1239, 1494
MwoI GCNNNNNNNGC 3 cut(s) 629, 742, 894
NciI CCSGG 2 cut(s) 98, 1459
NcoI CCATGG 1 cut(s) 1197
NdeI CATATG 1 cut(s) 1363
NlaIV GGNNCC 3 cut(s) 72, 856, 1063
NsiI ATGCAT 2 cut(s) 738, 1606
NspI RCATGY 3 cut(s) 311, 736, 1571
NspV TTCGAA 2 cut(s) 208, 923
PaeI GCATGC 1 cut(s) 736
PaeR7I CTCGAG 1 cut(s) 1394
PagI TCATGA 2 cut(s) 148, 199
PciI ACATGT 1 cut(s) 1567
PctI GAATGC 1 cut(s) 1287
PdmI GAANNNNTTC 2 cut(s) 569, 713
PfeI GAWTC 7 cut(s) 64, 88, 117, 1129, 1190, 1254, 1415
PfoI TCCNGGA 2 cut(s) 112, 649
PkrI GCNGC 3 cut(s) 631, 744, 1029
Ple19I CGATCG 1 cut(s) 957
PleI GAGTC 5 cut(s) 358, 883, 958, 1241, 1386
PpsI GAGTC 5 cut(s) 358, 883, 958, 1241, 1386
PscI ACATGT 1 cut(s) 1567
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 504
Psp124BI GAGCTC 2 cut(s) 804, 1578
Psp1406I AACGTT 1 cut(s) 467
Psp6I CCWGG 5 cut(s) 112, 649, 888, 1237, 1492
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 5 cut(s) 112, 649, 888, 1237, 1492
PspN4I GGNNCC 3 cut(s) 72, 856, 1063
PspPI GGNCC 2 cut(s) 472, 1062
PspXI VCTCGAGB 1 cut(s) 1394
PstI CTGCAG 1 cut(s) 462
PsuI RGATCY 2 cut(s) 183, 1120
PvuI CGATCG 1 cut(s) 957
PvuII CAGCTG 2 cut(s) 742, 935
RsaI GTAC 2 cut(s) 359, 609
RsaNI GTAC 2 cut(s) 358, 608
RseI CAYNNNNRTG 2 cut(s) 1156, 1386
SacI GAGCTC 2 cut(s) 804, 1578
SaqAI TTAA 6 cut(s) 528, 792, 969, 1041, 1116, 1535
SatI GCNGC 3 cut(s) 630, 743, 1028
Sau96I GGNCC 2 cut(s) 472, 1062
SchI GAGTC 5 cut(s) 359, 884, 959, 1242, 1386
ScrFI CCNGG 7 cut(s) 98, 114, 651, 890, 1239, 1459, 1494
SduI GDGCHC 5 cut(s) 649, 804, 994, 1248, 1578
SfaNI GCATC 3 cut(s) 745, 1098, 1591
SfcI CTRYAG 2 cut(s) 458, 883
Sfr274I CTCGAG 1 cut(s) 1394
SfuI TTCGAA 2 cut(s) 208, 923
SinI GGWCC 2 cut(s) 472, 1062
SlaI CTCGAG 1 cut(s) 1394
SmiMI CAYNNNNRTG 2 cut(s) 1156, 1386
SmlI CTYRAG 4 cut(s) 288, 872, 1394, 1512
SmoI CTYRAG 4 cut(s) 288, 872, 1394, 1512
SphI GCATGC 1 cut(s) 736
Sse9I AATT 6 cut(s) 129, 312, 613, 1043, 1367, 1376
SsiI CCGC 2 cut(s) 442, 1202
SspMI CTAG 2 cut(s) 107, 1070
SstI GAGCTC 2 cut(s) 804, 1578
StyD4I CCNGG 7 cut(s) 96, 112, 649, 888, 1237, 1457, 1492
StyI CCWWGG 1 cut(s) 1197
TaaI ACNGT 6 cut(s) 499, 884, 1180, 1271, 1468, 1540
TaiI ACGT 2 cut(s) 172, 470
TaqI TCGA 9 cut(s) 208, 226, 597, 640, 665, 923, 953, 957, 1395
TasI AATT 6 cut(s) 129, 312, 613, 1043, 1367, 1376
TatI WGTACW 2 cut(s) 357, 607
TfiI GAWTC 7 cut(s) 64, 88, 117, 1129, 1190, 1254, 1415
Tru1I TTAA 6 cut(s) 528, 792, 969, 1041, 1116, 1535
Tru9I TTAA 6 cut(s) 528, 792, 969, 1041, 1116, 1535
TseI GCWGC 3 cut(s) 629, 742, 1027
TspDTI ATGAA 2 cut(s) 562, 1380
TspGWI ACGGA 2 cut(s) 774, 1396
VpaK11BI GGWCC 2 cut(s) 472, 1062
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 3 cut(s) 1043, 1367, 1376
XbaI TCTAGA 1 cut(s) 1069
XceI RCATGY 3 cut(s) 311, 736, 1571
XhoI CTCGAG 1 cut(s) 1394
XmiI GTMKAC 1 cut(s) 1561
XmnI GAANNNNTTC 2 cut(s) 569, 713
XspI CTAG 2 cut(s) 107, 1070
Zsp2I ATGCAT 2 cut(s) 738, 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.