Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0016182

Physical Location & Seq

Genomic Coordinates Forward (+)

33001544 .. 33006212

Gene Structure 4669 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1233900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGGCGATGCTGTTGGAGGATATTTTGAAGTCGGTGGAGCTCTGGTTGAAGCTCATCAAGAAGCCCCAGCCCTACGTGGACCCTCATCTCGACCCGGTTCTGCTGGTTCCTGGTATCGCCGGTTCGGTCTTGAACGCTGTCGATGAAGAGAGTGGTGCGGAGGAGCGCGTCTGGGTTCGGATCCTTAGTGCTGACTACACGTTTCGGACCAAGCTTTGGTCTCGTTTCGATCCTTCAACAGGTAAAACTGAGTCTATGGATCCAAAAGCAAGGATAGTGGTCCCTGAAGGTAGATATGGACTTCAGGCAATTGACGCCTTGGACCCTGACATGGTGATTGGACAGGAAGGTGTATATTATTTCCATGACATGATTGTTGAATTGACCAAGTGGGGTTTCCAAGAGGGGAAAACAATTTTTGGGTTTGGATATGATTTCCGCCAAAGCAACAGGCTGCAGGAGACATTGGATCGCTTAGCTGAAAAATTAGAGGCAGTGTATGAGGCTTCTGGAGGGAAAAAGATAACCATCATAACTCATTCTATGGGGGGCCTTCTTGTGAAATGTTTCATGTGCTTGCATAGTGATGTTTTTGAGAAATACGTGAACAAGTGGATTGCAATTGCTGCACCATTCCAGGGTGCACCTGGATATGTCACATCTACCTTTTTAAATGGAATGTCATTTGTTGATGGGTGGGAACAGAATTTTTTTATATCAAAATGGAGCATGCACCAACTGCTTATTGAGTGCCCATCAATATACGAGTTGATGGCTTGTCTTAATTTTCAATGGGAACATCCTCCGCTTCTGGAAATGTGGAGAGGTAGGCCTGATGGTGACGGGAAATCTCAAATTATGCTAGAGTCTTACCCCCTGGTAGAGAGTGTACAGATTTTTAAGGAAGCTCTTGCAAATAATACGGTCAATCATAACGGTGAAGATATTCCCCTACCATTTAATATGGAAATCTTGAAATGGGCTAATGAAACACGCAAGATCATATCTCAGGCGAAACTTCCTCCTCAAGTTAAATTCTACAATATATATGGGATGAATCTCGAGACACCTCATAGTGTTTGCTATGGGAATGAAGGAACACCTGTCACAGATCTACAACAGCTGCGATATTTCCAGCCTAATTATGTATGCGTTGATGGTGATGGGACAGTCCCAGTGGAATCTGCTAAGGCAGATGGGCTTAATGCTGCCGCAAGGGTTGGAGTCCCTGGCGAGCACCGAGGAATTCTCTGTGAGCACCATGTATTCCGGATACTGAAGCACTGGCTGAAGGCAGACCATGACCCGTACTACAACCCTGTAAACGATTATGTGATTCTACCCACTGCATTTGAAATGGAGAAGCACAAAGAGAAAGGTCTGGAAGTAACTTCTCTAAAAGAGGAGTGGGAAATCATCTCAGAATGCCAAGACGCTGATCACAAGAATTCTCATTCAGCCGCCGCTGATGAAAAGCCTGTGGTGAGTTCCATATCCGTTTCTGAAGTGGGAGCTGAGGCCTGCGCAACTCTTACTGTTCACCCTCAGAGCGAGGGAAAGCAGCATGTGGAGCTGAATGCTCTAAGTGTGTCAGTCGATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009056 GO:0009395 GO:0009987 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046434 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110

Pfam Domains

Protein Families

LCAT

Protein Analysis

535

Amino Acids

60.13

Weight (kDa)

5.12

Isoelectric Point (pI)

36.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LCAT	PF02450	76 - 397	9.3e-28	Lecithin:cholesterol acyltransferase
DUF676	PF05057	158 - 245	6.6e-06	Putative serine esterase (DUF676)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19860
fragaria_vesca	FvH4_3g12200
malus_domestica	MD05G1252800.v1.1 MD10G1233900.v1.1
prunus_persica	Prupe.4G111100_v2.0.a1
pyrus_communis	pycom05g23100 pycom10g19620
rosa_chinensis	RchiOBHm_Chr5g0020001
rosa_laevigata	RLG00000032492
rosa_multiflora	Rmu_sc0002250.1_g000005
rosa_roxburghii	Rroxscaffold_1G00058190
rosa_rugosa	Rorug05G0056100
rosa_samantha	Rh5AG146300 Rh5BG145800
rosa_wichuraiana	Rw5G012920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1525
AccB7I	CCANNNNNTGG	1 cut(s)	216
AccII	CGCG	1 cut(s)	168
AccIII	TCCGGA	1 cut(s)	1269
AciI	CCGC	6 cut(s)	158, 439, 806, 1212, 1461, 1464
AclWI	GGATC	6 cut(s)	175, 188, 224, 254, 267, 477
AcsI	RAATTY	4 cut(s)	706, 1034, 1245, 1447
AcuI	CTGAAG	5 cut(s)	287, 306, 1298, 1310, 1524
AcyI	GRCGYC	1 cut(s)	315
AfaI	GTAC	2 cut(s)	891, 1310
AfiI	CCNNNNNNNGG	4 cut(s)	216, 239, 331, 638
AflIII	ACRYGT	1 cut(s)	198
AgsI	TTSAA	8 cut(s)	28, 49, 133, 237, 380, 791, 976, 1355
AjnI	CCWGG	5 cut(s)	109, 636, 646, 876, 1228
AjuI	GAANNNNNNNTTGG	2 cut(s)	380, 412
AluBI	AGCT	8 cut(s)	40, 52, 214, 479, 908, 1123, 1514, 1573
AluI	AGCT	8 cut(s)	40, 52, 214, 479, 908, 1123, 1514, 1573
Alw21I	GWGCWC	4 cut(s)	42, 646, 1239, 1260
Alw26I	GTCTC	3 cut(s)	225, 455, 1058
Alw44I	GTGCAC	1 cut(s)	642
AlwI	GGATC	6 cut(s)	175, 188, 224, 254, 267, 477
Ama87I	CYCGRG	1 cut(s)	1061
Aor13HI	TCCGGA	1 cut(s)	1269
AoxI	GGCC	3 cut(s)	550, 830, 1518
ApaLI	GTGCAC	1 cut(s)	642
ApeKI	GCWGC	5 cut(s)	454, 626, 1123, 1208, 1561
ApoI	RAATTY	4 cut(s)	706, 1034, 1245, 1447
Asp700I	GAANNNNTTC	2 cut(s)	566, 945
AspLEI	GCGC	2 cut(s)	168, 1526
AspS9I	GGNCC	5 cut(s)	79, 207, 280, 322, 550
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	6 cut(s)	346, 851, 950, 1172, 1495, 1532
AvaI	CYCGRG	1 cut(s)	1061
AvaII	GGWCC	4 cut(s)	79, 207, 280, 322
BaeGI	GKGCMC	2 cut(s)	646, 755
BamHI	GGATCC	2 cut(s)	180, 259
BanII	GRGCYC	1 cut(s)	42
Bbv12I	GWGCWC	4 cut(s)	42, 646, 1239, 1260
BbvCI	CCTCAGC	1 cut(s)	1515
BbvI	GCAGC	5 cut(s)	441, 613, 1110, 1195, 1573
BccI	CCATC	8 cut(s)	536, 686, 763, 766, 830, 1151, 1157, 1190
BciT130I	CCWGG	5 cut(s)	111, 638, 648, 878, 1230
BciVI	GTATCC	1 cut(s)	1266
BclI	TGATCA	1 cut(s)	1438
BcnI	CCSGG	1 cut(s)	95
BcoDI	GTCTC	3 cut(s)	225, 455, 1058
BfaI	CTAG	1 cut(s)	863
BfmI	CTRYAG	1 cut(s)	455
BfuI	GTATCC	1 cut(s)	1266
BglII	AGATCT	1 cut(s)	1111
BisI	GCNGC	8 cut(s)	455, 627, 1124, 1209, 1212, 1461, 1464, 1562
BlpI	GCTNAGC	1 cut(s)	475
BlsI	GCNGC	8 cut(s)	456, 628, 1125, 1210, 1213, 1462, 1465, 1563
Bme1390I	CCNGG	6 cut(s)	95, 111, 638, 648, 878, 1230
Bme18I	GGWCC	4 cut(s)	79, 207, 280, 322
BmeT110I	CYCGRG	1 cut(s)	1061
BmgT120I	GGNCC	5 cut(s)	79, 207, 280, 322, 550
BmiI	GGNNCC	7 cut(s)	81, 108, 182, 261, 282, 324, 551
BmrFI	CCNGG	6 cut(s)	95, 111, 638, 648, 878, 1230
BmrI	ACTGGG	1 cut(s)	1169
BmsI	GCATC	1 cut(s)	1588
BmuI	ACTGGG	1 cut(s)	1169
BplI	GAGNNNNNCTC	2 cut(s)	1233, 1265
BpmI	CTGGAG	1 cut(s)	531
Bpu10I	CCTNAGC	2 cut(s)	1188, 1515
Bpu1102I	GCTNAGC	1 cut(s)	475
BpuEI	CTTGAG	1 cut(s)	1011
BpuMI	CCSGG	1 cut(s)	95
BsaAI	YACGTR	2 cut(s)	76, 604
BsaBI	GATNNNNATC	1 cut(s)	527
BsaHI	GRCGYC	1 cut(s)	315
BsaI	GGTCTC	1 cut(s)	225
BsaJI	CCNNGG	5 cut(s)	318, 637, 876, 1228, 1240
BsaWI	WCCGGW	1 cut(s)	1269
BsaXI	ACNNNNNCTCC	2 cut(s)	29, 59
Bsc4I	CCNNNNNNNGG	4 cut(s)	216, 239, 331, 638
Bse118I	RCCGGY	1 cut(s)	119
Bse1I	ACTGG	2 cut(s)	1175, 1289
Bse8I	GATNNNNATC	1 cut(s)	527
BseAI	TCCGGA	1 cut(s)	1269
BseBI	CCWGG	5 cut(s)	111, 638, 648, 878, 1230
BseDI	CCNNGG	5 cut(s)	318, 637, 876, 1228, 1240
BseGI	GGATG	2 cut(s)	799, 1059
BseJI	GATNNNNATC	1 cut(s)	527
BseLI	CCNNNNNNNGG	4 cut(s)	216, 239, 331, 638
BseMII	CTCAG	5 cut(s)	240, 1022, 1434, 1506, 1559
BseNI	ACTGG	2 cut(s)	1175, 1289
BseRI	GAGGAG	3 cut(s)	176, 1014, 1418
BseSI	GKGCMC	2 cut(s)	646, 755
BseXI	GCAGC	5 cut(s)	441, 613, 1110, 1195, 1573
BseYI	CCCAGC	1 cut(s)	66
BsgI	GTGCAG	1 cut(s)	612
Bsh1236I	CGCG	1 cut(s)	168
BshFI	GGCC	3 cut(s)	552, 832, 1520
BsiHKAI	GWGCWC	4 cut(s)	42, 646, 1239, 1260
BsiHKCI	CYCGRG	1 cut(s)	1061
BsiSI	CCGG	3 cut(s)	95, 120, 1270
BslFI	GGGAC	4 cut(s)	266, 1157, 1180, 1211
BslI	CCNNNNNNNGG	4 cut(s)	216, 239, 331, 638
BsmAI	GTCTC	3 cut(s)	225, 455, 1058
BsmFI	GGGAC	4 cut(s)	266, 1157, 1180, 1211
BsmI	GAATGC	2 cut(s)	1430, 1582
BsnI	GGCC	3 cut(s)	552, 832, 1520
Bso31I	GGTCTC	1 cut(s)	225
BsoBI	CYCGRG	1 cut(s)	1061
Bsp1286I	GDGCHC	5 cut(s)	42, 646, 755, 1239, 1260
Bsp13I	TCCGGA	1 cut(s)	1269
Bsp1407I	TGTACA	1 cut(s)	889
Bsp143I	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
Bsp1720I	GCTNAGC	1 cut(s)	475
BspACI	CCGC	6 cut(s)	158, 439, 806, 1212, 1461, 1464
BspANI	GGCC	3 cut(s)	552, 832, 1520
BspCNI	CTCAG	5 cut(s)	241, 1021, 1433, 1507, 1558
BspEI	TCCGGA	1 cut(s)	1269
BspFNI	CGCG	1 cut(s)	168
BspLI	GGNNCC	7 cut(s)	81, 108, 182, 261, 282, 324, 551
BspMAI	CTGCAG	1 cut(s)	459
BspPI	GGATC	6 cut(s)	175, 188, 224, 254, 267, 477
BspTNI	GGTCTC	1 cut(s)	225
BsrFI	RCCGGY	1 cut(s)	119
BsrGI	TGTACA	1 cut(s)	889
BsrI	ACTGG	2 cut(s)	1175, 1289
BssAI	RCCGGY	1 cut(s)	119
BssECI	CCNNGG	5 cut(s)	318, 637, 876, 1228, 1240
BssMI	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
BssNI	GRCGYC	1 cut(s)	315
BssT1I	CCWWGG	1 cut(s)	318
Bst2UI	CCWGG	5 cut(s)	111, 638, 648, 878, 1230
Bst4CI	ACNGT	4 cut(s)	925, 938, 1171, 1537
Bst6I	CTCTTC	1 cut(s)	141
BstACI	GRCGYC	1 cut(s)	315
BstAPI	GCANNNNNTGC	2 cut(s)	626, 739
BstAUI	TGTACA	1 cut(s)	889
BstBAI	YACGTR	2 cut(s)	76, 604
BstC8I	GCNNGC	4 cut(s)	578, 731, 1235, 1522
BstDEI	CTNAG	9 cut(s)	185, 249, 475, 1008, 1188, 1420, 1515, 1545, 1583
BstENI	CCTNNNNNAGG	1 cut(s)	237
BstF5I	GGATG	2 cut(s)	799, 1059
BstFNI	CGCG	1 cut(s)	168
BstHHI	GCGC	2 cut(s)	168, 1526
BstKTI	GATC	7 cut(s)	183, 232, 262, 472, 1002, 1114, 1441
BstMAI	GTCTC	3 cut(s)	225, 455, 1058
BstMBI	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
BstMWI	GCNNNNNNNGC	4 cut(s)	314, 626, 739, 1570
BstNI	CCWGG	5 cut(s)	111, 638, 648, 878, 1230
BstNSI	RCATGY	2 cut(s)	733, 1568
BstSCI	CCNGG	6 cut(s)	93, 109, 636, 646, 876, 1228
BstSFI	CTRYAG	1 cut(s)	455
BstSLI	GKGCMC	2 cut(s)	646, 755
BstUI	CGCG	1 cut(s)	168
BstV1I	GCAGC	5 cut(s)	441, 613, 1110, 1195, 1573
BstX2I	RGATCY	3 cut(s)	180, 259, 1111
BstYI	RGATCY	3 cut(s)	180, 259, 1111
BsuI	GTATCC	1 cut(s)	1266
BsuRI	GGCC	3 cut(s)	552, 832, 1520
BtgZI	GCGATG	1 cut(s)	20
BtsCI	GGATG	2 cut(s)	799, 1059
BtsI	GCAGTG	2 cut(s)	501, 1344
BtsIMutI	CAGTG	4 cut(s)	501, 1182, 1282, 1344
Cac8I	GCNNGC	4 cut(s)	578, 731, 1235, 1522
CfoI	GCGC	2 cut(s)	168, 1526
Cfr10I	RCCGGY	1 cut(s)	119
Cfr13I	GGNCC	5 cut(s)	79, 207, 280, 322, 550
CseI	GACGC	3 cut(s)	157, 323, 1442
Csp6I	GTAC	2 cut(s)	890, 1309
CspCI	CAANNNNNGTGG	4 cut(s)	258, 293, 1333, 1368
CviAII	CATG	8 cut(s)	331, 365, 370, 571, 730, 1262, 1301, 1565
CviQI	GTAC	2 cut(s)	890, 1309
DdeI	CTNAG	9 cut(s)	185, 249, 475, 1008, 1188, 1420, 1515, 1545, 1583
DpnI	GATC	7 cut(s)	182, 231, 261, 471, 1001, 1113, 1440
DpnII	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
DraI	TTTAAA	1 cut(s)	672
Eam1104I	CTCTTC	1 cut(s)	141
EarI	CTCTTC	1 cut(s)	141
EciI	GGCGGA	1 cut(s)	428
Ecl136II	GAGCTC	1 cut(s)	40
Eco130I	CCWWGG	1 cut(s)	318
Eco147I	AGGCCT	2 cut(s)	832, 1520
Eco24I	GRGCYC	1 cut(s)	42
Eco31I	GGTCTC	1 cut(s)	225
Eco47I	GGWCC	4 cut(s)	79, 207, 280, 322
Eco53kI	GAGCTC	1 cut(s)	40
Eco57I	CTGAAG	5 cut(s)	287, 306, 1298, 1310, 1524
Eco88I	CYCGRG	1 cut(s)	1061
EcoICRI	GAGCTC	1 cut(s)	40
EcoNI	CCTNNNNNAGG	1 cut(s)	237
EcoO109I	RGGNCCY	1 cut(s)	550
EcoRI	GAATTC	2 cut(s)	1245, 1447
EcoRII	CCWGG	5 cut(s)	109, 636, 646, 876, 1228
EcoT14I	CCWWGG	1 cut(s)	318
EcoT38I	GRGCYC	1 cut(s)	42
ErhI	CCWWGG	1 cut(s)	318
FaeI	CATG	8 cut(s)	334, 368, 373, 574, 733, 1265, 1304, 1568
FaqI	GGGAC	4 cut(s)	266, 1157, 1180, 1211
FatI	CATG	8 cut(s)	330, 364, 369, 570, 729, 1261, 1300, 1564
FbaI	TGATCA	1 cut(s)	1438
Fnu4HI	GCNGC	8 cut(s)	455, 627, 1124, 1209, 1212, 1461, 1464, 1562
FokI	GGATG	2 cut(s)	786, 1066
FriOI	GRGCYC	1 cut(s)	42
Fsp4HI	GCNGC	8 cut(s)	455, 627, 1124, 1209, 1212, 1461, 1464, 1562
FspBI	CTAG	1 cut(s)	863
FspI	TGCGCA	1 cut(s)	1525
GlaI	GCGC	2 cut(s)	167, 1525
GluI	GCNGC	8 cut(s)	455, 627, 1124, 1209, 1212, 1461, 1464, 1562
GsaI	CCCAGC	1 cut(s)	70
GsuI	CTGGAG	1 cut(s)	531
HaeIII	GGCC	3 cut(s)	552, 832, 1520
HapII	CCGG	3 cut(s)	95, 120, 1270
HgaI	GACGC	3 cut(s)	157, 323, 1442
HhaI	GCGC	2 cut(s)	168, 1526
Hin1I	GRCGYC	1 cut(s)	315
Hin1II	CATG	8 cut(s)	334, 368, 373, 574, 733, 1265, 1304, 1568
Hin6I	GCGC	2 cut(s)	166, 1524
HinP1I	GCGC	2 cut(s)	166, 1524
HindIII	AAGCTT	1 cut(s)	212
HinfI	GANTC	6 cut(s)	251, 866, 1057, 1181, 1224, 1336
HpaII	CCGG	3 cut(s)	95, 120, 1270
HphI	GGTGA	6 cut(s)	346, 851, 950, 1172, 1495, 1532
Hpy166II	GTNNAC	6 cut(s)	79, 607, 644, 890, 1324, 1540
Hpy188I	TCNGA	5 cut(s)	180, 207, 1423, 1504, 1548
Hpy8I	GTNNAC	6 cut(s)	79, 607, 644, 890, 1324, 1540
HpyAV	CCTTC	6 cut(s)	243, 281, 341, 563, 1088, 1285
HpyCH4III	ACNGT	4 cut(s)	925, 938, 1171, 1537
HpyCH4IV	ACGT	3 cut(s)	75, 200, 603
HpyCH4V	TGCA	8 cut(s)	457, 580, 620, 629, 644, 733, 914, 1349
HpyF10VI	GCNNNNNNNGC	4 cut(s)	314, 626, 739, 1570
HpyF3I	CTNAG	9 cut(s)	185, 249, 475, 1008, 1188, 1420, 1515, 1545, 1583
HpySE526I	ACGT	3 cut(s)	75, 200, 603
Hsp92I	GRCGYC	1 cut(s)	315
Hsp92II	CATG	8 cut(s)	334, 368, 373, 574, 733, 1265, 1304, 1568
HspAI	GCGC	2 cut(s)	166, 1524
Kpn2I	TCCGGA	1 cut(s)	1269
Ksp22I	TGATCA	1 cut(s)	1438
Kzo9I	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
LmnI	GCTCC	5 cut(s)	37, 163, 726, 1511, 1570
Lsp1109I	GCAGC	5 cut(s)	441, 613, 1110, 1195, 1573
LweI	GCATC	1 cut(s)	1588
MaeI	CTAG	1 cut(s)	863
MaeII	ACGT	3 cut(s)	75, 200, 603
MaeIII	GTNAC	4 cut(s)	655, 839, 1105, 1387
MalI	GATC	7 cut(s)	182, 231, 261, 471, 1001, 1113, 1440
MboI	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
MboII	GAAGA	2 cut(s)	158, 953
MfeI	CAATTG	2 cut(s)	309, 621
MflI	RGATCY	3 cut(s)	180, 259, 1111
MhlI	GDGCHC	5 cut(s)	42, 646, 755, 1239, 1260
MlyI	GAGTC	3 cut(s)	260, 875, 1233
MmeI	TCCRAC	1 cut(s)	1201
MroI	TCCGGA	1 cut(s)	1269
MroXI	GAANNNNTTC	2 cut(s)	566, 945
MseI	TTAA	6 cut(s)	671, 783, 900, 960, 1032, 1203
MslI	CAYNNNNRTG	2 cut(s)	585, 936
MspA1I	CMGCKG	2 cut(s)	1123, 1466
MspI	CCGG	3 cut(s)	95, 120, 1270
MspR9I	CCNGG	6 cut(s)	95, 111, 638, 648, 878, 1230
MunI	CAATTG	2 cut(s)	309, 621
Mva1269I	GAATGC	2 cut(s)	1430, 1582
MvaI	CCWGG	5 cut(s)	111, 638, 648, 878, 1230
MvnI	CGCG	1 cut(s)	168
MwoI	GCNNNNNNNGC	4 cut(s)	314, 626, 739, 1570
NciI	CCSGG	1 cut(s)	95
NdeII	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
NlaIII	CATG	8 cut(s)	334, 368, 373, 574, 733, 1265, 1304, 1568
NlaIV	GGNNCC	7 cut(s)	81, 108, 182, 261, 282, 324, 551
NmuCI	GTSAC	3 cut(s)	655, 839, 1105
NsbI	TGCGCA	1 cut(s)	1525
NspI	RCATGY	2 cut(s)	733, 1568
PaeI	GCATGC	1 cut(s)	733
PaeR7I	CTCGAG	1 cut(s)	1061
PceI	AGGCCT	2 cut(s)	832, 1520
PctI	GAATGC	2 cut(s)	1430, 1582
PdmI	GAANNNNTTC	2 cut(s)	566, 945
PfeI	GAWTC	3 cut(s)	1057, 1181, 1336
PflMI	CCANNNNNTGG	1 cut(s)	216
PkrI	GCNGC	8 cut(s)	456, 628, 1125, 1210, 1213, 1462, 1465, 1563
PleI	GAGTC	3 cut(s)	259, 874, 1232
PpsI	GAGTC	3 cut(s)	259, 874, 1232
Ppu21I	YACGTR	2 cut(s)	76, 604
Psp124BI	GAGCTC	1 cut(s)	42
Psp6I	CCWGG	5 cut(s)	109, 636, 646, 876, 1228
PspFI	CCCAGC	1 cut(s)	66
PspGI	CCWGG	5 cut(s)	109, 636, 646, 876, 1228
PspN4I	GGNNCC	7 cut(s)	81, 108, 182, 261, 282, 324, 551
PspPI	GGNCC	5 cut(s)	79, 207, 280, 322, 550
PsrI	GAACNNNNNNTAC	2 cut(s)	106, 138
PstI	CTGCAG	1 cut(s)	459
PsuI	RGATCY	3 cut(s)	180, 259, 1111
PvuII	CAGCTG	1 cut(s)	1123
RsaI	GTAC	2 cut(s)	891, 1310
RsaNI	GTAC	2 cut(s)	890, 1309
RseI	CAYNNNNRTG	2 cut(s)	585, 936
SacI	GAGCTC	1 cut(s)	42
SaqAI	TTAA	6 cut(s)	671, 783, 900, 960, 1032, 1203
SatI	GCNGC	8 cut(s)	455, 627, 1124, 1209, 1212, 1461, 1464, 1562
Sau3AI	GATC	7 cut(s)	180, 229, 259, 469, 999, 1111, 1438
Sau96I	GGNCC	5 cut(s)	79, 207, 280, 322, 550
SchI	GAGTC	3 cut(s)	260, 875, 1233
ScrFI	CCNGG	6 cut(s)	95, 111, 638, 648, 878, 1230
SduI	GDGCHC	5 cut(s)	42, 646, 755, 1239, 1260
SfaNI	GCATC	1 cut(s)	1588
SfcI	CTRYAG	1 cut(s)	455
Sfr274I	CTCGAG	1 cut(s)	1061
SinI	GGWCC	4 cut(s)	79, 207, 280, 322
SlaI	CTCGAG	1 cut(s)	1061
SmiMI	CAYNNNNRTG	2 cut(s)	585, 936
SmlI	CTYRAG	2 cut(s)	1026, 1061
SmoI	CTYRAG	2 cut(s)	1026, 1061
SphI	GCATGC	1 cut(s)	733
SseBI	AGGCCT	2 cut(s)	832, 1520
SsiI	CCGC	6 cut(s)	158, 439, 806, 1212, 1461, 1464
SspMI	CTAG	1 cut(s)	863
SstI	GAGCTC	1 cut(s)	42
StuI	AGGCCT	2 cut(s)	832, 1520
StyD4I	CCNGG	6 cut(s)	93, 109, 636, 646, 876, 1228
StyI	CCWWGG	1 cut(s)	318
TaaI	ACNGT	4 cut(s)	925, 938, 1171, 1537
TaiI	ACGT	3 cut(s)	78, 203, 606
TaqI	TCGA	5 cut(s)	90, 141, 228, 1062, 1596
TaqII	GACCGA	1 cut(s)	115
TatI	WGTACW	1 cut(s)	889
TauI	GCSGC	3 cut(s)	1214, 1463, 1466
TfiI	GAWTC	3 cut(s)	1057, 1181, 1336
Tru1I	TTAA	6 cut(s)	671, 783, 900, 960, 1032, 1203
Tru9I	TTAA	6 cut(s)	671, 783, 900, 960, 1032, 1203
TscAI	CASTG	4 cut(s)	501, 1182, 1289, 1351
TseFI	GTSAC	3 cut(s)	655, 839, 1105
TseI	GCWGC	5 cut(s)	454, 626, 1123, 1208, 1561
Tsp45I	GTSAC	3 cut(s)	655, 839, 1105
TspDTI	ATGAA	6 cut(s)	159, 559, 1002, 1070, 1107, 1485
TspGWI	ACGGA	1 cut(s)	1486
TspRI	CASTG	4 cut(s)	501, 1182, 1289, 1351
Van91I	CCANNNNNTGG	1 cut(s)	216
VneI	GTGCAC	1 cut(s)	642
VpaK11BI	GGWCC	4 cut(s)	79, 207, 280, 322
XagI	CCTNNNNNAGG	1 cut(s)	237
XapI	RAATTY	4 cut(s)	706, 1034, 1245, 1447
XceI	RCATGY	2 cut(s)	733, 1568
XcmI	CCANNNNNNNNNTGG	1 cut(s)	644
XhoI	CTCGAG	1 cut(s)	1061
XmnI	GAANNNNTTC	2 cut(s)	566, 945
XspI	CTAG	1 cut(s)	863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.