Protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 105, 494 |
| AciI | CCGC | 1 cut(s) | 105 |
| AcsI | RAATTY | 3 cut(s) | 128, 176, 325 |
| AcuI | CTGAAG | 1 cut(s) | 80 |
| AgsI | TTSAA | 4 cut(s) | 25, 42, 112, 386 |
| AluBI | AGCT | 3 cut(s) | 224, 482, 610 |
| AluI | AGCT | 3 cut(s) | 224, 482, 610 |
| Alw26I | GTCTC | 3 cut(s) | 306, 321, 494 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 86 |
| ApoI | RAATTY | 3 cut(s) | 128, 176, 325 |
| AsuII | TTCGAA | 1 cut(s) | 172 |
| BbsI | GAAGAC | 2 cut(s) | 86, 363 |
| BccI | CCATC | 2 cut(s) | 223, 280 |
| BcoDI | GTCTC | 3 cut(s) | 306, 321, 494 |
| BmiI | GGNNCC | 1 cut(s) | 229 |
| BmsI | GCATC | 1 cut(s) | 531 |
| BoxI | GACNNNNGTC | 1 cut(s) | 313 |
| BpiI | GAAGAC | 2 cut(s) | 86, 363 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 149, 181 |
| Bpu14I | TTCGAA | 1 cut(s) | 172 |
| BpuEI | CTTGAG | 1 cut(s) | 468 |
| BsaI | GGTCTC | 2 cut(s) | 306, 494 |
| BsaJI | CCNNGG | 1 cut(s) | 446 |
| BsaWI | WCCGGW | 1 cut(s) | 230 |
| Bse1I | ACTGG | 1 cut(s) | 287 |
| Bse3DI | GCAATG | 1 cut(s) | 440 |
| BseDI | CCNNGG | 1 cut(s) | 446 |
| BseGI | GGATG | 2 cut(s) | 291, 546 |
| BseMI | GCAATG | 1 cut(s) | 440 |
| BseMII | CTCAG | 2 cut(s) | 78, 550 |
| BseNI | ACTGG | 1 cut(s) | 287 |
| Bsh1236I | CGCG | 2 cut(s) | 105, 494 |
| BsiSI | CCGG | 1 cut(s) | 231 |
| BsmAI | GTCTC | 3 cut(s) | 306, 321, 494 |
| BsmI | GAATGC | 1 cut(s) | 158 |
| Bso31I | GGTCTC | 2 cut(s) | 306, 494 |
| Bsp119I | TTCGAA | 1 cut(s) | 172 |
| Bsp143I | GATC | 1 cut(s) | 507 |
| Bsp19I | CCATGG | 1 cut(s) | 446 |
| BspACI | CCGC | 1 cut(s) | 105 |
| BspCNI | CTCAG | 2 cut(s) | 79, 549 |
| BspFNI | CGCG | 2 cut(s) | 105, 494 |
| BspLI | GGNNCC | 1 cut(s) | 229 |
| BspT104I | TTCGAA | 1 cut(s) | 172 |
| BspTNI | GGTCTC | 2 cut(s) | 306, 494 |
| BsrDI | GCAATG | 1 cut(s) | 440 |
| BsrI | ACTGG | 1 cut(s) | 287 |
| BssECI | CCNNGG | 1 cut(s) | 446 |
| BssMI | GATC | 1 cut(s) | 507 |
| BssT1I | CCWWGG | 1 cut(s) | 446 |
| Bst4CI | ACNGT | 5 cut(s) | 11, 312, 350, 413, 583 |
| Bst6I | CTCTTC | 1 cut(s) | 429 |
| BstBI | TTCGAA | 1 cut(s) | 172 |
| BstDEI | CTNAG | 2 cut(s) | 87, 536 |
| BstDSI | CCRYGG | 1 cut(s) | 446 |
| BstF5I | GGATG | 2 cut(s) | 291, 546 |
| BstFNI | CGCG | 2 cut(s) | 105, 494 |
| BstKTI | GATC | 1 cut(s) | 510 |
| BstMAI | GTCTC | 3 cut(s) | 306, 321, 494 |
| BstMBI | GATC | 1 cut(s) | 507 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 562 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 313 |
| BstUI | CGCG | 2 cut(s) | 105, 494 |
| BstV2I | GAAGAC | 2 cut(s) | 86, 363 |
| BtgI | CCRYGG | 1 cut(s) | 446 |
| BtsCI | GGATG | 2 cut(s) | 291, 546 |
| CaiI | CAGNNNCTG | 1 cut(s) | 86 |
| CviAII | CATG | 2 cut(s) | 250, 447 |
| CviJI | RGCY | 7 cut(s) | 55, 189, 224, 470, 482, 519, 610 |
| CviKI_1 | RGCY | 7 cut(s) | 55, 189, 224, 470, 482, 519, 610 |
| DdeI | CTNAG | 2 cut(s) | 87, 536 |
| DpnI | GATC | 1 cut(s) | 509 |
| DpnII | GATC | 1 cut(s) | 507 |
| Eam1104I | CTCTTC | 1 cut(s) | 429 |
| EarI | CTCTTC | 1 cut(s) | 429 |
| Eco130I | CCWWGG | 1 cut(s) | 446 |
| Eco31I | GGTCTC | 2 cut(s) | 306, 494 |
| Eco57I | CTGAAG | 1 cut(s) | 80 |
| EcoT14I | CCWWGG | 1 cut(s) | 446 |
| EcoT22I | ATGCAT | 1 cut(s) | 546 |
| ErhI | CCWWGG | 1 cut(s) | 446 |
| FaeI | CATG | 2 cut(s) | 253, 450 |
| FaiI | YATR | 5 cut(s) | 194, 251, 448, 546, 554 |
| FatI | CATG | 2 cut(s) | 249, 446 |
| FokI | GGATG | 2 cut(s) | 298, 553 |
| HapII | CCGG | 1 cut(s) | 231 |
| Hin1II | CATG | 2 cut(s) | 253, 450 |
| HindIII | AAGCTT | 1 cut(s) | 608 |
| HinfI | GANTC | 4 cut(s) | 83, 91, 100, 234 |
| HpaII | CCGG | 1 cut(s) | 231 |
| Hpy166II | GTNNAC | 1 cut(s) | 266 |
| Hpy188I | TCNGA | 6 cut(s) | 82, 88, 99, 134, 324, 425 |
| Hpy188III | TCNNGA | 4 cut(s) | 219, 511, 538, 591 |
| Hpy8I | GTNNAC | 1 cut(s) | 266 |
| HpyAV | CCTTC | 1 cut(s) | 48 |
| HpyCH4III | ACNGT | 5 cut(s) | 11, 312, 350, 413, 583 |
| HpyCH4V | TGCA | 3 cut(s) | 212, 544, 575 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 562 |
| HpyF3I | CTNAG | 2 cut(s) | 87, 536 |
| Hsp92II | CATG | 2 cut(s) | 253, 450 |
| Kzo9I | GATC | 1 cut(s) | 507 |
| LmnI | GCTCC | 1 cut(s) | 52 |
| LpnPI | CCDG | 5 cut(s) | 127, 210, 244, 268, 523 |
| LweI | GCATC | 1 cut(s) | 531 |
| MaeIII | GTNAC | 2 cut(s) | 344, 413 |
| MalI | GATC | 1 cut(s) | 509 |
| MboI | GATC | 1 cut(s) | 507 |
| MboII | GAAGA | 7 cut(s) | 86, 124, 364, 368, 443, 446, 491 |
| MfeI | CAATTG | 1 cut(s) | 42 |
| MluCI | AATT | 5 cut(s) | 42, 128, 176, 325, 334 |
| MlyI | GAGTC | 1 cut(s) | 100 |
| MnlI | CCTC | 5 cut(s) | 66, 78, 151, 291, 430 |
| Mph1103I | ATGCAT | 1 cut(s) | 546 |
| MseI | TTAA | 1 cut(s) | 462 |
| MslI | CAYNNNNRTG | 1 cut(s) | 445 |
| MspI | CCGG | 1 cut(s) | 231 |
| MunI | CAATTG | 1 cut(s) | 42 |
| Mva1269I | GAATGC | 1 cut(s) | 158 |
| MvnI | CGCG | 2 cut(s) | 105, 494 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 562 |
| NcoI | CCATGG | 1 cut(s) | 446 |
| NdeII | GATC | 1 cut(s) | 507 |
| NlaIII | CATG | 2 cut(s) | 253, 450 |
| NlaIV | GGNNCC | 1 cut(s) | 229 |
| NmuCI | GTSAC | 1 cut(s) | 413 |
| NsiI | ATGCAT | 1 cut(s) | 546 |
| NspV | TTCGAA | 1 cut(s) | 172 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 153 |
| PctI | GAATGC | 1 cut(s) | 158 |
| PfeI | GAWTC | 3 cut(s) | 83, 100, 234 |
| PflFI | GACNNNGTC | 1 cut(s) | 143 |
| PleI | GAGTC | 1 cut(s) | 99 |
| PpsI | GAGTC | 1 cut(s) | 99 |
| PshAI | GACNNNNGTC | 1 cut(s) | 313 |
| PspN4I | GGNNCC | 1 cut(s) | 229 |
| PstNI | CAGNNNCTG | 1 cut(s) | 86 |
| PsyI | GACNNNGTC | 1 cut(s) | 143 |
| RseI | CAYNNNNRTG | 1 cut(s) | 445 |
| SaqAI | TTAA | 1 cut(s) | 462 |
| Sau3AI | GATC | 1 cut(s) | 507 |
| SchI | GAGTC | 1 cut(s) | 100 |
| SetI | ASST | 5 cut(s) | 226, 302, 484, 490, 612 |
| SfaNI | GCATC | 1 cut(s) | 531 |
| SfuI | TTCGAA | 1 cut(s) | 172 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 445 |
| SmlI | CTYRAG | 1 cut(s) | 483 |
| SmoI | CTYRAG | 1 cut(s) | 483 |
| Sse9I | AATT | 5 cut(s) | 42, 128, 176, 325, 334 |
| SsiI | CCGC | 1 cut(s) | 105 |
| SspI | AATATT | 1 cut(s) | 75 |
| StyI | CCWWGG | 1 cut(s) | 446 |
| TaaI | ACNGT | 5 cut(s) | 11, 312, 350, 413, 583 |
| TaqI | TCGA | 3 cut(s) | 172, 506, 592 |
| TasI | AATT | 5 cut(s) | 42, 128, 176, 325, 334 |
| TfiI | GAWTC | 3 cut(s) | 83, 100, 234 |
| Tru1I | TTAA | 1 cut(s) | 462 |
| Tru9I | TTAA | 1 cut(s) | 462 |
| TseFI | GTSAC | 1 cut(s) | 413 |
| Tsp45I | GTSAC | 1 cut(s) | 413 |
| TspDTI | ATGAA | 1 cut(s) | 213 |
| Tth111I | GACNNNGTC | 1 cut(s) | 143 |
| XapI | RAATTY | 3 cut(s) | 128, 176, 325 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 608 |
| Zsp2I | ATGCAT | 1 cut(s) | 546 |