AT5G20540

BREVIS RADIX-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010246
Physical Location & Seq
Forward (+)
6947789 .. 6952038
4250 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20540.1

Sequence Viewer

Length: 1155 bp
ATGCTGACGTGTATAGCTCGTTCGAAGCGAGCAGGCGATGAATCCTCCGGTCAACCAGACGATCCAGATTCTAAAAACGCCAAATCTCTAACATCTCAGCTCAAAGATATGGCTCTGAAAGCATCAGGAGCTTACCGGCATTGTACGCCGTGTACGGCGGCACAAGGTCAGGGACAAGGACAAGGTCCGATCAAGAACAATCCGTCGTCGTCGTCGGTAAAGTCGGATTTCGAATCGGATCAACGGTTTAAAATGCTTTACGGAAGATCAAACAGTTCGATTACAGCTACGGCGGCGGTGGCGGCGACGCAACAACAACAGCCTAGGGTTTGGGGAAAGGAGATGGAAGCGAGACTAAAAGGGATTTCGAGCGGCGAAGCGACTCCGAAATCGGCGAGTGGGAGAAACCGGGTCGACCCGATTGTGTTTGTGGAGGAGAAAGAGCCTAAAGAATGGGTTGCTCAGGTTGAGCCCGGAGTTCTCATAACCTTCGTTTCTCTTCCCGGCGGTGGTAATGATCTCAAGCGGATACGTTTCAGCCGAGACATGTTCAACAAGTTACAAGCTCAACGATGGTGGGCAGATAACTATGACAAAGTAATGGAACTTTACAATGTTCAAAAACTAAGCCGCCAAGCTTTCCCGCTTCCCACCCCGCCTAGATCCGAAGACGAGAATGCAAAAGTGGAGTACCATCCAGAAGACACTCCTGCAACACCGCCTCTAAACAAAGAACGGTTGCCTCGTACTATCCATCGTCCACCTGGATTGGCTGCTTACTCATCCTCAGATTCACTCGACCATAATTCAATGCAGAGCCAGCAGTTCTATGACTCTGGTCTACTCAACTCAACTCCTAAAGTTTCAAGCATCAGTGTAGCCAAGACAGAAACTTCTTCCATAGATGCTTCCATAAGAAGCAGCTCGTCGAGAGATGCAGACCGGTCAGAGGAAATGTCGGTAAGCAATGCGAGTGATGTTGACAACGAATGGGTGGAGCAAGATGAGCCTGGCGTTTATATCACCATTAAAGTTTTACCAGGTGGGAAAAGAGAGCTTCGAAGAGTCAGATTCAGCCGAGAGAGATTCGGGGAGATGCACGCGAGATTATGGTGGGAAGAGAACAGGGCAAGGATACATGAACAATACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N

Protein Analysis

384

Amino Acids

42.88

Weight (kDa)

8.28

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRX_N PF13713 28 - 51 1.7e-11 Transcription factor BRX N-terminal domain
BRX PF08381 150 - 204 7.8e-25 Transcription factor regulating root and shoot growth via Pin3
BRX PF08381 329 - 383 9e-29 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 2 cut(s) 414, 841
AccII CGCG 1 cut(s) 1103
AclWI GGATC 3 cut(s) 56, 246, 657
AfaI GTAC 4 cut(s) 145, 154, 692, 748
AfiI CCNNNNNNNGG 2 cut(s) 509, 949
AflIII ACRYGT 2 cut(s) 8, 546
AgeI ACCGGT 1 cut(s) 942
AgsI TTSAA 4 cut(s) 553, 620, 810, 867
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 3 cut(s) 763, 1009, 1039
AluBI AGCT 8 cut(s) 17, 100, 131, 287, 566, 638, 924, 1057
AluI AGCT 8 cut(s) 17, 100, 131, 287, 566, 638, 924, 1057
Alw26I GTCTC 2 cut(s) 346, 537
AlwI GGATC 3 cut(s) 56, 246, 657
ApeKI GCWGC 2 cut(s) 773, 921
AsiGI ACCGGT 1 cut(s) 942
AspA2I CCTAGG 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 3 cut(s) 410, 474, 504
AsuHPI GGTGA 1 cut(s) 1015
AsuII TTCGAA 3 cut(s) 23, 231, 1060
AvaII GGWCC 1 cut(s) 185
AvrII CCTAGG 1 cut(s) 323
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 2 cut(s) 675, 708
BbvI GCAGC 2 cut(s) 760, 933
BccI CCATC 4 cut(s) 337, 567, 702, 762
BceAI ACGGC 3 cut(s) 133, 171, 306
BciT130I CCWGG 3 cut(s) 765, 1011, 1041
BciVI GTATCC 2 cut(s) 522, 1128
BcnI CCSGG 3 cut(s) 410, 474, 504
BcoDI GTCTC 2 cut(s) 346, 537
BfaI CTAG 2 cut(s) 324, 660
BfuI GTATCC 2 cut(s) 522, 1128
BisI GCNGC 7 cut(s) 159, 294, 303, 373, 631, 774, 922
BlnI CCTAGG 1 cut(s) 323
BlsI GCNGC 7 cut(s) 160, 295, 304, 374, 632, 775, 923
Bme1390I CCNGG 6 cut(s) 410, 474, 504, 765, 1011, 1041
Bme18I GGWCC 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 6 cut(s) 410, 474, 504, 765, 1011, 1041
BmsI GCATC 5 cut(s) 131, 879, 895, 925, 1086
BoxI GACNNNNGTC 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 675, 708
Bpu10I CCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 3 cut(s) 23, 231, 1060
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 3 cut(s) 410, 474, 504
BsaJI CCNNGG 1 cut(s) 323
BsaWI WCCGGW 2 cut(s) 47, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 949
Bse118I RCCGGY 2 cut(s) 135, 942
Bse3DI GCAATG 1 cut(s) 973
BseBI CCWGG 3 cut(s) 765, 1011, 1041
BseDI CCNNGG 1 cut(s) 323
BseGI GGATG 2 cut(s) 694, 782
BseLI CCNNNNNNNGG 2 cut(s) 509, 949
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 3 cut(s) 110, 476, 801
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 760, 933
Bsh1236I CGCG 1 cut(s) 1103
BshTI ACCGGT 1 cut(s) 942
BsiSI CCGG 6 cut(s) 48, 136, 409, 474, 504, 943
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 2 cut(s) 509, 949
BsmAI GTCTC 2 cut(s) 346, 537
BsmFI GGGAC 1 cut(s) 186
BsmI GAATGC 1 cut(s) 682
Bsp119I TTCGAA 3 cut(s) 23, 231, 1060
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 6 cut(s) 61, 189, 238, 266, 517, 662
BspCNI CTCAG 3 cut(s) 109, 475, 800
BspFNI CGCG 1 cut(s) 1103
BspPI GGATC 3 cut(s) 56, 246, 657
BspT104I TTCGAA 3 cut(s) 23, 231, 1060
BsrBI CCGCTC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 973
BsrFI RCCGGY 2 cut(s) 135, 942
BssAI RCCGGY 2 cut(s) 135, 942
BssECI CCNNGG 1 cut(s) 323
BssMI GATC 6 cut(s) 61, 189, 238, 266, 517, 662
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 3 cut(s) 765, 1011, 1041
Bst4CI ACNGT 3 cut(s) 246, 275, 738
Bst6I CTCTTC 3 cut(s) 504, 1057, 1113
BstBI TTCGAA 3 cut(s) 23, 231, 1060
BstC8I GCNNGC 4 cut(s) 30, 34, 821, 1101
BstDEI CTNAG 4 cut(s) 96, 462, 626, 787
BstF5I GGATG 2 cut(s) 694, 782
BstFNI CGCG 1 cut(s) 1103
BstKTI GATC 6 cut(s) 64, 192, 241, 269, 520, 665
BstMAI GTCTC 2 cut(s) 346, 537
BstMBI GATC 6 cut(s) 61, 189, 238, 266, 517, 662
BstMWI GCNNNNNNNGC 8 cut(s) 119, 128, 145, 293, 299, 302, 820, 1006
BstNI CCWGG 3 cut(s) 765, 1011, 1041
BstNSI RCATGY 1 cut(s) 550
BstPAI GACNNNNGTC 1 cut(s) 837
BstSCI CCNGG 6 cut(s) 408, 472, 502, 763, 1009, 1039
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 2 cut(s) 760, 933
BstV2I GAAGAC 2 cut(s) 675, 708
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BsuI GTATCC 2 cut(s) 522, 1128
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 2 cut(s) 694, 782
BtsIMutI CAGTG 1 cut(s) 880
Cac8I GCNNGC 4 cut(s) 30, 34, 821, 1101
Cfr10I RCCGGY 2 cut(s) 135, 942
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 1 cut(s) 316
CsiI ACCWGGT 1 cut(s) 1039
Csp6I GTAC 4 cut(s) 144, 153, 691, 747
CspAI ACCGGT 1 cut(s) 942
CspCI CAANNNNNGTGG 4 cut(s) 557, 592, 750, 785
CviAII CATG 2 cut(s) 547, 1139
CviQI GTAC 4 cut(s) 144, 153, 691, 747
DdeI CTNAG 4 cut(s) 96, 462, 626, 787
DpnI GATC 6 cut(s) 63, 191, 240, 268, 519, 664
DpnII GATC 6 cut(s) 61, 189, 238, 266, 517, 662
DraI TTTAAA 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 504, 1057, 1113
EarI CTCTTC 3 cut(s) 504, 1057, 1113
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 763, 1009, 1039
EcoT14I CCWWGG 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 2 cut(s) 550, 1142
FaqI GGGAC 1 cut(s) 186
FatI CATG 2 cut(s) 546, 1138
FauI CCCGC 2 cut(s) 651, 663
FblI GTMKAC 2 cut(s) 414, 841
Fnu4HI GCNGC 7 cut(s) 159, 294, 303, 373, 631, 774, 922
FokI GGATG 2 cut(s) 681, 769
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 7 cut(s) 159, 294, 303, 373, 631, 774, 922
FspBI CTAG 2 cut(s) 324, 660
GluI GCNGC 7 cut(s) 159, 294, 303, 373, 631, 774, 922
HapII CCGG 6 cut(s) 48, 136, 409, 474, 504, 943
HgaI GACGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 550, 1142
HincII GTYRAC 3 cut(s) 53, 415, 982
HindII GTYRAC 3 cut(s) 53, 415, 982
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 9 cut(s) 41, 68, 233, 382, 791, 833, 1065, 1071, 1086
HpaII CCGG 6 cut(s) 48, 136, 409, 474, 504, 943
HphI GGTGA 1 cut(s) 1015
Hpy166II GTNNAC 6 cut(s) 53, 153, 415, 761, 842, 982
Hpy188I TCNGA 9 cut(s) 117, 189, 226, 238, 387, 667, 790, 949, 1070
Hpy188III TCNNGA 5 cut(s) 65, 126, 193, 698, 930
Hpy8I GTNNAC 6 cut(s) 53, 153, 415, 761, 842, 982
Hpy99I CGWCG 6 cut(s) 208, 211, 214, 217, 310, 931
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 3 cut(s) 246, 275, 738
HpyCH4IV ACGT 2 cut(s) 8, 532
HpyCH4V TGCA 5 cut(s) 680, 713, 814, 938, 1099
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 128, 145, 293, 299, 302, 820, 1006
HpyF3I CTNAG 4 cut(s) 96, 462, 626, 787
HpySE526I ACGT 2 cut(s) 8, 532
Hsp92II CATG 2 cut(s) 550, 1142
Kzo9I GATC 6 cut(s) 61, 189, 238, 266, 517, 662
LmnI GCTCC 2 cut(s) 128, 997
Lsp1109I GCAGC 2 cut(s) 760, 933
LweI GCATC 5 cut(s) 131, 879, 895, 925, 1086
MabI ACCWGGT 1 cut(s) 1039
MaeI CTAG 2 cut(s) 324, 660
MaeII ACGT 2 cut(s) 8, 532
MaeIII GTNAC 1 cut(s) 558
MalI GATC 6 cut(s) 63, 191, 240, 268, 519, 664
MbiI CCGCTC 1 cut(s) 372
MboI GATC 6 cut(s) 61, 189, 238, 266, 517, 662
MboII GAAGA 7 cut(s) 276, 491, 680, 713, 888, 1074, 1130
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 1 cut(s) 805
MlyI GAGTC 3 cut(s) 376, 827, 1074
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 6 cut(s) 55, 427, 732, 753, 796, 943
MseI TTAA 2 cut(s) 249, 1029
MspI CCGG 6 cut(s) 48, 136, 409, 474, 504, 943
MspR9I CCNGG 6 cut(s) 410, 474, 504, 765, 1011, 1041
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 3 cut(s) 765, 1011, 1041
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 8 cut(s) 119, 128, 145, 293, 299, 302, 820, 1006
NciI CCSGG 3 cut(s) 410, 474, 504
NdeII GATC 6 cut(s) 61, 189, 238, 266, 517, 662
NlaIII CATG 2 cut(s) 550, 1142
NmeAIII GCCGAG 2 cut(s) 566, 1103
NspI RCATGY 1 cut(s) 550
NspV TTCGAA 3 cut(s) 23, 231, 1060
PciI ACATGT 1 cut(s) 546
PcsI WCGNNNNNNNCGW 3 cut(s) 25, 538, 742
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 6 cut(s) 41, 68, 233, 791, 1071, 1086
PflFI GACNNNGTC 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 942
PkrI GCNGC 7 cut(s) 160, 295, 304, 374, 632, 775, 923
PleI GAGTC 3 cut(s) 376, 827, 1073
PpsI GAGTC 3 cut(s) 376, 827, 1073
PscI ACATGT 1 cut(s) 546
PshAI GACNNNNGTC 1 cut(s) 837
Psp6I CCWGG 3 cut(s) 763, 1009, 1039
PspGI CCWGG 3 cut(s) 763, 1009, 1039
PspPI GGNCC 1 cut(s) 185
PsrI GAACNNNNNNTAC 1 cut(s) 36
PsuI RGATCY 1 cut(s) 662
PsyI GACNNNGTC 1 cut(s) 183
RsaI GTAC 4 cut(s) 145, 154, 692, 748
RsaNI GTAC 4 cut(s) 144, 153, 691, 747
SalI GTCGAC 1 cut(s) 413
SaqAI TTAA 2 cut(s) 249, 1029
SatI GCNGC 7 cut(s) 159, 294, 303, 373, 631, 774, 922
Sau3AI GATC 6 cut(s) 61, 189, 238, 266, 517, 662
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 3 cut(s) 376, 827, 1074
ScrFI CCNGG 6 cut(s) 410, 474, 504, 765, 1011, 1041
SduI GDGCHC 1 cut(s) 474
SexAI ACCWGGT 1 cut(s) 1039
SfaNI GCATC 5 cut(s) 131, 879, 895, 925, 1086
SfuI TTCGAA 3 cut(s) 23, 231, 1060
SinI GGWCC 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 1 cut(s) 805
SspMI CTAG 2 cut(s) 324, 660
StyD4I CCNGG 6 cut(s) 408, 472, 502, 763, 1009, 1039
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 3 cut(s) 246, 275, 738
TaiI ACGT 2 cut(s) 11, 535
TaqI TCGA 8 cut(s) 23, 231, 278, 368, 414, 798, 929, 1060
TasI AATT 1 cut(s) 805
TauI GCSGC 5 cut(s) 161, 296, 305, 375, 633
TfiI GAWTC 6 cut(s) 41, 68, 233, 791, 1071, 1086
Tru1I TTAA 2 cut(s) 249, 1029
Tru9I TTAA 2 cut(s) 249, 1029
TscAI CASTG 1 cut(s) 880
TseI GCWGC 2 cut(s) 773, 921
TspDTI ATGAA 2 cut(s) 54, 1155
TspGWI ACGGA 2 cut(s) 192, 276
TspRI CASTG 1 cut(s) 880
Tth111I GACNNNGTC 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 761
XmaJI CCTAGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 414, 841
XspI CTAG 2 cut(s) 324, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.