AT5G20950

Lysosomal beta glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011666
Physical Location & Seq
Reverse (-)
7107272 .. 7111453
4182 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20950.3

Sequence Viewer

Length: 1875 bp
ATGGGGACTTTGAGTAAGGTGCTATGTCTCATGCTGCTTTGCTGTATCGTGGCTGCTGCTGAGGGTACTTTGAAGTACAAGGACCCAAAGCAGCCTTTGGGTGCAAGAATCAGAGATTTGATGAACCGTATGACTCTACAAGAGAAGATTGGTCAGATGGTTCAGATCGAACGCAGCGTCGCTACACCTGAAGTCATGAAGAAATACTTCATTGGGAGTGTTTTGAGTGGTGGAGGAAGTGTGCCCTCGGAAAAAGCTACTCCTGAAACTTGGGTGAACATGGTCAATGAGATTCAAAAGGCGTCTCTTTCGACCCGCCTTGGGATCCCTATGATCTACGGGATTGATGCTGTTCACGGTCACAACAATGTGTATGGCGCCACCATTTTCCCGCACAATGTAGGCCTTGGAGTCACCAGGGATCCTAACCTTGTTAAGAGGATTGGGGCAGCAACCGCGCTTGAAGTCAGAGCGACTGGAATCCCATATGCCTTTGCCCCGTGTATTGCAGTTTGTAGGGATCCAAGATGGGGAAGATGCTACGAGAGCTACAGCGAGGATTATAGAATTGTCCAACAGATGACTGAGATTATACCCGGTTTGCAAGGCGACCTTCCTACCAAGCGAAAGGGTGTTCCCTTTGTTGGTGGAAAGACAAAAGTTGCAGCTTGTGCAAAGCATTTTGTTGGAGATGGAGGTACAGTGAGAGGAATTGATGAGAACAACACAGTGATTGACTCGAAGGGCCTGTTCGGAATTCACATGCCTGGATATTACAATGCTGTAAACAAGGGTGTTGCAACAATAATGGTGTCTTACTCTGCCTGGAACGGTTTGAGAATGCATGCTAACAAGGAACTTGTCACGGGTTTCCTCAAGAACAAGTTGAAGTTCAGGGGTTTTGTCATCTCTGATTGGCAGGGGATTGATAGGATCACGACTCCTCCTCATCTTAACTATTCATACTCTGTTTACGCGGGAATCAGTGCTGGAATTGACATGATTATGGTTCCGTACAACTACACTGAGTTCATAGACGAAATCAGCAGTCAGATACAGAAAAAGCTTATTCCGATTAGCAGGATCGATGACGCCCTGAAGAGAATCTTAAGGGTCAAATTCACAATGGGACTCTTTGAGGAACCACTGGCTGATCTCAGCTTTGCCAACCAGCTTGGTAGCAAGGAACATAGGGAACTAGCTCGTGAAGCCGTGAGGAAGTCTCTGGTGCTGCTCAAGAATGGTAAGACAGGTGCTAAACCTCTGCTTCCTCTGCCTAAGAAATCAGGGAAGATCCTTGTCGCGGGAGCACATGCTGATAACTTGGGATATCAATGTGGTGGCTGGACCATCACCTGGCAAGGCCTTAATGGCAACGACCATACCGTTGGTACAACAATCCTAGCGGCTGTGAAGAATACAGTGGCTCCTACCACACAAGTCGTCTACAGCCAAAACCCCGATGCGAACTTTGTGAAGTCGGGTAAATTTGACTATGCCATTGTGGTAGTCGGGGAGCCACCTTATGCTGAAATGTTTGGGGACACCACGAACCTGACCATAAGTGATCCTGGTCCGAGCATAATAGGAAACGTGTGCGGATCAGTGAAATGTGTGGTGGTTGTGGTCTCTGGCCGTCCCGTGGTGATTCAGCCTTATGTTTCGACCATTGACGCTCTCGTGGCGGCTTGGCTTCCGGGAACAGAAGGTCAAGGAGTGGCTGATGCTCTGTTTGGTGATTACGGGTTCACAGGAAAGTTGGCCCGGACGTGGTTTAAGTCGGTGAAGCAGCTACCGATGAATGTTGGAGATCGGCATTACGACCCATTGTACCCATTCGGTTTCGGGTTGACCACAAAACCATACAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

624

Amino Acids

67.93

Weight (kDa)

9.23

Isoelectric Point (pI)

24.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 123 - 450 1e-68 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 487 - 696 2.5e-37 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011666)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20940 AT5G20950 AT5G20950 AT5G20950
fragaria_vesca FvH4_3g26961
malus_domestica MD03G1161800.v1.1 MD11G1178000.v1.1
prunus_persica Prupe.6G150800_v2.0.a1 Prupe.6G150800_v2.0.a1
pyrus_communis pycom11g15210
rosa_chinensis RchiOBHm_Chr5g0049481
rosa_laevigata RLG00000034621
rosa_multiflora Rmu_sc0015493.1_g000005
rosa_roxburghii Rroxscaffold_1G00032210
rosa_rugosa Rorug05G0248700
rosa_samantha Rh5AG325200 Rh5BG336300 Rh5DG348500
rosa_wichuraiana Rw5G030700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 1356
AccI GTMKAC 1 cut(s) 1446
AccII CGCG 3 cut(s) 458, 977, 1304
AciI CCGC 8 cut(s) 316, 392, 456, 977, 1304, 1406, 1599, 1685
AcoI YGGCCR 1 cut(s) 1633
AcsI RAATTY 3 cut(s) 756, 1118, 1487
AcuI CTGAAG 2 cut(s) 210, 1118
AcyI GRCGYC 3 cut(s) 302, 378, 1092
AfaI GTAC 6 cut(s) 67, 77, 700, 1016, 1393, 1832
AfiI CCNNNNNNNGG 7 cut(s) 321, 530, 644, 1303, 1356, 1642, 1770
AflII CTTAAG 1 cut(s) 1108
AflIII ACRYGT 1 cut(s) 1593
AgsI TTSAA 4 cut(s) 73, 296, 464, 889
AjiI CACGTC 1 cut(s) 1770
AjnI CCWGG 5 cut(s) 416, 766, 824, 1355, 1570
AluBI AGCT 8 cut(s) 257, 549, 668, 1066, 1161, 1174, 1202, 1792
AluI AGCT 8 cut(s) 257, 549, 668, 1066, 1161, 1174, 1202, 1792
Alw21I GWGCWC 1 cut(s) 1312
Alw26I GTCTC 4 cut(s) 32, 309, 1227, 1633
AoxI GGCC 5 cut(s) 403, 745, 1363, 1633, 1761
ApeKI GCWGC 9 cut(s) 34, 53, 56, 91, 174, 449, 665, 1231, 1789
ApoI RAATTY 3 cut(s) 756, 1118, 1487
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 2 cut(s) 380, 460
AspS9I GGNCC 5 cut(s) 82, 745, 1347, 1574, 1762
AsuC2I CCSGG 3 cut(s) 597, 1698, 1765
AsuHPI GGTGA 6 cut(s) 286, 406, 1345, 1657, 1748, 1795
AvaII GGWCC 3 cut(s) 82, 1347, 1574
BaeGI GKGCMC 1 cut(s) 246
BamHI GGATCC 3 cut(s) 324, 421, 520
BanI GGYRCC 1 cut(s) 377
BauI CACGAG 2 cut(s) 1203, 1679
Bbv12I GWGCWC 1 cut(s) 1312
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 9 cut(s) 21, 40, 43, 103, 186, 461, 677, 1218, 1801
BccI CCATC 4 cut(s) 151, 522, 686, 1358
BceAI ACGGC 2 cut(s) 1196, 1620
BciT130I CCWGG 5 cut(s) 418, 768, 826, 1357, 1572
BcnI CCSGG 3 cut(s) 597, 1698, 1765
BcoDI GTCTC 4 cut(s) 32, 309, 1227, 1633
BfaI CTAG 2 cut(s) 1199, 1403
BfmI CTRYAG 2 cut(s) 550, 1447
BfoI RGCGCY 1 cut(s) 381
BfrI CTTAAG 1 cut(s) 1108
BglI GCCNNNNNGGC 1 cut(s) 1371
Bme1390I CCNGG 8 cut(s) 418, 597, 768, 826, 1357, 1572, 1698, 1765
Bme18I GGWCC 3 cut(s) 82, 1347, 1574
BmgBI CACGTC 1 cut(s) 1770
BmgT120I GGNCC 5 cut(s) 82, 745, 1347, 1574, 1762
BmiI GGNNCC 9 cut(s) 84, 326, 379, 423, 522, 1011, 1143, 1428, 1518
BmrFI CCNGG 8 cut(s) 418, 597, 768, 826, 1357, 1572, 1698, 1765
BmsI GCATC 4 cut(s) 337, 527, 1453, 1714
BplI GAGNNNNNCTC 2 cut(s) 1207, 1239
Bpu10I CCTNAGC 1 cut(s) 60
BpuEI CTTGAG 2 cut(s) 860, 1220
BpuMI CCSGG 3 cut(s) 597, 1698, 1765
Bsa29I ATCGAT 1 cut(s) 1086
BsaHI GRCGYC 3 cut(s) 302, 378, 1092
BsaI GGTCTC 1 cut(s) 1633
BsaJI CCNNGG 5 cut(s) 246, 319, 406, 417, 1641
BsaXI ACNNNNNCTCC 8 cut(s) 225, 255, 687, 717, 928, 958, 1411, 1441
Bsc4I CCNNNNNNNGG 7 cut(s) 321, 530, 644, 1303, 1356, 1642, 1770
Bse1I ACTGG 2 cut(s) 481, 1152
BseBI CCWGG 5 cut(s) 418, 768, 826, 1357, 1572
BseCI ATCGAT 1 cut(s) 1086
BseDI CCNNGG 5 cut(s) 246, 319, 406, 417, 1641
BseLI CCNNNNNNNGG 7 cut(s) 321, 530, 644, 1303, 1356, 1642, 1770
BseMII CTCAG 4 cut(s) 51, 576, 1017, 1171
BseNI ACTGG 2 cut(s) 481, 1152
BseRI GAGGAG 2 cut(s) 933, 936
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 9 cut(s) 21, 40, 43, 103, 186, 461, 677, 1218, 1801
Bsh1236I CGCG 3 cut(s) 458, 977, 1304
BshFI GGCC 5 cut(s) 405, 747, 1365, 1635, 1763
BshNI GGYRCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 1086
BsiHKAI GWGCWC 1 cut(s) 1312
BsiSI CCGG 3 cut(s) 597, 1697, 1765
BslFI GGGAC 4 cut(s) 19, 1143, 1556, 1623
BslI CCNNNNNNNGG 7 cut(s) 321, 530, 644, 1303, 1356, 1642, 1770
BsmAI GTCTC 4 cut(s) 32, 309, 1227, 1633
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 4 cut(s) 19, 1143, 1556, 1623
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 5 cut(s) 405, 747, 1365, 1635, 1763
Bso31I GGTCTC 1 cut(s) 1633
Bsp1286I GDGCHC 2 cut(s) 246, 1312
BspACI CCGC 8 cut(s) 316, 392, 456, 977, 1304, 1406, 1599, 1685
BspANI GGCC 5 cut(s) 405, 747, 1365, 1635, 1763
BspCNI CTCAG 4 cut(s) 52, 577, 1018, 1170
BspDI ATCGAT 1 cut(s) 1086
BspFNI CGCG 3 cut(s) 458, 977, 1304
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 9 cut(s) 84, 326, 379, 423, 522, 1011, 1143, 1428, 1518
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 1 cut(s) 1633
BsrI ACTGG 2 cut(s) 481, 1152
BssECI CCNNGG 5 cut(s) 246, 319, 406, 417, 1641
BssNI GRCGYC 3 cut(s) 302, 378, 1092
BssSI CACGAG 2 cut(s) 1203, 1679
BssT1I CCWWGG 2 cut(s) 319, 406
Bst2BI CACGAG 2 cut(s) 1203, 1679
Bst2UI CCWGG 5 cut(s) 418, 768, 826, 1357, 1572
Bst4CI ACNGT 7 cut(s) 128, 359, 703, 730, 833, 1387, 1423
Bst6I CTCTTC 1 cut(s) 1094
BstACI GRCGYC 3 cut(s) 302, 378, 1092
BstAFI CTTAAG 1 cut(s) 1108
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 846
BstDEI CTNAG 5 cut(s) 60, 585, 1026, 1157, 1278
BstDSI CCRYGG 1 cut(s) 1641
BstFNI CGCG 3 cut(s) 458, 977, 1304
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 2 cut(s) 380, 460
BstMAI GTCTC 4 cut(s) 32, 309, 1227, 1633
BstMWI GCNNNNNNNGC 7 cut(s) 455, 546, 671, 1208, 1273, 1371, 1682
BstNI CCWGG 5 cut(s) 418, 768, 826, 1357, 1572
BstNSI RCATGY 3 cut(s) 766, 848, 1316
BstSCI CCNGG 8 cut(s) 416, 595, 766, 824, 1355, 1570, 1696, 1763
BstSFI CTRYAG 2 cut(s) 550, 1447
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 3 cut(s) 458, 977, 1304
BstV1I GCAGC 9 cut(s) 21, 40, 43, 103, 186, 461, 677, 1218, 1801
BstX2I RGATCY 4 cut(s) 324, 421, 520, 1293
BstXI CCANNNNNNTGG 1 cut(s) 1388
BstYI RGATCY 4 cut(s) 324, 421, 520, 1293
Bsu15I ATCGAT 1 cut(s) 1086
BsuRI GGCC 5 cut(s) 405, 747, 1365, 1635, 1763
BsuTUI ATCGAT 1 cut(s) 1086
BtgI CCRYGG 1 cut(s) 1641
BtrI CACGTC 1 cut(s) 1770
BtsIMutI CAGTG 7 cut(s) 708, 735, 991, 1023, 1145, 1428, 1611
Cac8I GCNNGC 1 cut(s) 846
CciI TCATGA 1 cut(s) 195
CfoI GCGC 2 cut(s) 380, 460
Cfr13I GGNCC 5 cut(s) 82, 745, 1347, 1574, 1762
ClaI ATCGAT 1 cut(s) 1086
CseI GACGC 4 cut(s) 166, 291, 1100, 1682
Csp6I GTAC 6 cut(s) 66, 76, 699, 1015, 1392, 1831
CviAII CATG 7 cut(s) 31, 196, 280, 763, 845, 1000, 1313
CviQI GTAC 6 cut(s) 66, 76, 699, 1015, 1392, 1831
DdeI CTNAG 5 cut(s) 60, 585, 1026, 1157, 1278
DinI GGCGCC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 1633
Eam1104I CTCTTC 1 cut(s) 1094
EarI CTCTTC 1 cut(s) 1094
Eco130I CCWWGG 2 cut(s) 319, 406
Eco147I AGGCCT 2 cut(s) 405, 1365
Eco31I GGTCTC 1 cut(s) 1633
Eco32I GATATC 1 cut(s) 1331
Eco47I GGWCC 3 cut(s) 82, 1347, 1574
Eco57I CTGAAG 2 cut(s) 210, 1118
EcoO109I RGGNCCY 2 cut(s) 82, 745
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 5 cut(s) 416, 766, 824, 1355, 1570
EcoRV GATATC 1 cut(s) 1331
EcoT14I CCWWGG 2 cut(s) 319, 406
EcoT22I ATGCAT 1 cut(s) 846
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 319, 406
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 7 cut(s) 34, 199, 283, 766, 848, 1003, 1316
FalI AAGNNNNNCTT 6 cut(s) 191, 223, 597, 629, 1091, 1123
FaqI GGGAC 4 cut(s) 19, 1143, 1556, 1623
FatI CATG 7 cut(s) 30, 195, 279, 762, 844, 999, 1312
FauI CCCGC 4 cut(s) 323, 399, 970, 1297
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 1446
FspBI CTAG 2 cut(s) 1199, 1403
GlaI GCGC 2 cut(s) 379, 459
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 5 cut(s) 405, 747, 1365, 1635, 1763
HapII CCGG 3 cut(s) 597, 1697, 1765
HgaI GACGC 4 cut(s) 166, 291, 1100, 1682
HhaI GCGC 2 cut(s) 380, 460
Hin1I GRCGYC 3 cut(s) 302, 378, 1092
Hin1II CATG 7 cut(s) 34, 199, 283, 766, 848, 1003, 1316
Hin6I GCGC 2 cut(s) 378, 458
HinP1I GCGC 2 cut(s) 378, 458
HincII GTYRAC 1 cut(s) 1851
HindII GTYRAC 1 cut(s) 1851
HindIII AAGCTT 1 cut(s) 1064
HpaII CCGG 3 cut(s) 597, 1697, 1765
HphI GGTGA 6 cut(s) 286, 406, 1345, 1657, 1748, 1795
Hpy166II GTNNAC 7 cut(s) 277, 355, 787, 973, 1447, 1749, 1851
Hpy188III TCNNGA 6 cut(s) 196, 263, 877, 937, 1205, 1237
Hpy8I GTNNAC 7 cut(s) 277, 355, 787, 973, 1447, 1749, 1851
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 623, 736, 1700
HpyCH4III ACNGT 7 cut(s) 128, 359, 703, 730, 833, 1387, 1423
HpyCH4IV ACGT 2 cut(s) 1593, 1769
HpyCH4V TGCA 7 cut(s) 104, 509, 604, 665, 674, 800, 844
HpyF10VI GCNNNNNNNGC 7 cut(s) 455, 546, 671, 1208, 1273, 1371, 1682
HpyF3I CTNAG 5 cut(s) 60, 585, 1026, 1157, 1278
HpySE526I ACGT 2 cut(s) 1593, 1769
Hsp92I GRCGYC 3 cut(s) 302, 378, 1092
Hsp92II CATG 7 cut(s) 34, 199, 283, 766, 848, 1003, 1316
HspAI GCGC 2 cut(s) 378, 458
KasI GGCGCC 1 cut(s) 377
LmnI GCTCC 3 cut(s) 1307, 1432, 1516
Lsp1109I GCAGC 9 cut(s) 21, 40, 43, 103, 186, 461, 677, 1218, 1801
LweI GCATC 4 cut(s) 337, 527, 1453, 1714
MaeI CTAG 2 cut(s) 1199, 1403
MaeII ACGT 2 cut(s) 1593, 1769
MaeIII GTNAC 3 cut(s) 359, 412, 862
MboII GAAGA 6 cut(s) 157, 211, 546, 1111, 1303, 1426
MflI RGATCY 4 cut(s) 324, 421, 520, 1293
MhlI GDGCHC 2 cut(s) 246, 1312
MluCI AATT 6 cut(s) 567, 711, 756, 993, 1118, 1487
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 5 cut(s) 127, 420, 731, 934, 1125
MmeI TCCRAC 3 cut(s) 598, 667, 1786
Mph1103I ATGCAT 1 cut(s) 846
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 5 cut(s) 435, 954, 1109, 1368, 1776
MslI CAYNNNNRTG 2 cut(s) 366, 1004
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 3 cut(s) 597, 1697, 1765
MspR9I CCNGG 8 cut(s) 418, 597, 768, 826, 1357, 1572, 1698, 1765
Mva1269I GAATGC 1 cut(s) 846
MvaI CCWGG 5 cut(s) 418, 768, 826, 1357, 1572
MvnI CGCG 3 cut(s) 458, 977, 1304
MwoI GCNNNNNNNGC 7 cut(s) 455, 546, 671, 1208, 1273, 1371, 1682
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 3 cut(s) 597, 1698, 1765
NdeI CATATG 1 cut(s) 487
NlaIII CATG 7 cut(s) 34, 199, 283, 766, 848, 1003, 1316
NlaIV GGNNCC 9 cut(s) 84, 326, 379, 423, 522, 1011, 1143, 1428, 1518
NmuCI GTSAC 3 cut(s) 359, 412, 862
NsiI ATGCAT 1 cut(s) 846
NspI RCATGY 3 cut(s) 766, 848, 1316
PaeI GCATGC 1 cut(s) 848
PagI TCATGA 1 cut(s) 195
PceI AGGCCT 2 cut(s) 405, 1365
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 1383
PctI GAATGC 1 cut(s) 846
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 6 cut(s) 108, 292, 480, 981, 1104, 1648
PflMI CCANNNNNTGG 1 cut(s) 1356
PfoI TCCNGGA 1 cut(s) 1696
PleI GAGTC 5 cut(s) 127, 419, 731, 934, 1125
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 5 cut(s) 127, 419, 731, 934, 1125
PpuMI RGGWCCY 1 cut(s) 82
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 5 cut(s) 416, 766, 824, 1355, 1570
PspGI CCWGG 5 cut(s) 416, 766, 824, 1355, 1570
PspN4I GGNNCC 9 cut(s) 84, 326, 379, 423, 522, 1011, 1143, 1428, 1518
PspPI GGNCC 5 cut(s) 82, 745, 1347, 1574, 1762
PspPPI RGGWCCY 1 cut(s) 82
PsuI RGATCY 4 cut(s) 324, 421, 520, 1293
RsaI GTAC 6 cut(s) 67, 77, 700, 1016, 1393, 1832
RsaNI GTAC 6 cut(s) 66, 76, 699, 1015, 1392, 1831
RseI CAYNNNNRTG 2 cut(s) 366, 1004
SaqAI TTAA 5 cut(s) 435, 954, 1109, 1368, 1776
Sau96I GGNCC 5 cut(s) 82, 745, 1347, 1574, 1762
SchI GAGTC 5 cut(s) 127, 420, 731, 934, 1125
ScrFI CCNGG 8 cut(s) 418, 597, 768, 826, 1357, 1572, 1698, 1765
SduI GDGCHC 2 cut(s) 246, 1312
SfaNI GCATC 4 cut(s) 337, 527, 1453, 1714
SfcI CTRYAG 2 cut(s) 550, 1447
SfoI GGCGCC 1 cut(s) 379
SinI GGWCC 3 cut(s) 82, 1347, 1574
SmiMI CAYNNNNRTG 2 cut(s) 366, 1004
SmlI CTYRAG 3 cut(s) 875, 1108, 1235
SmoI CTYRAG 3 cut(s) 875, 1108, 1235
SphI GCATGC 1 cut(s) 848
Sse9I AATT 6 cut(s) 567, 711, 756, 993, 1118, 1487
SseBI AGGCCT 2 cut(s) 405, 1365
SsiI CCGC 8 cut(s) 316, 392, 456, 977, 1304, 1406, 1599, 1685
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 2 cut(s) 1199, 1403
StuI AGGCCT 2 cut(s) 405, 1365
StyD4I CCNGG 8 cut(s) 416, 595, 766, 824, 1355, 1570, 1696, 1763
StyI CCWWGG 2 cut(s) 319, 406
TaaI ACNGT 7 cut(s) 128, 359, 703, 730, 833, 1387, 1423
TaiI ACGT 2 cut(s) 1596, 1772
TaqI TCGA 5 cut(s) 168, 311, 740, 1086, 1664
TasI AATT 6 cut(s) 567, 711, 756, 993, 1118, 1487
TatI WGTACW 1 cut(s) 75
TauI GCSGC 2 cut(s) 1409, 1688
TfiI GAWTC 6 cut(s) 108, 292, 480, 981, 1104, 1648
Tru1I TTAA 5 cut(s) 435, 954, 1109, 1368, 1776
Tru9I TTAA 5 cut(s) 435, 954, 1109, 1368, 1776
TscAI CASTG 7 cut(s) 708, 735, 991, 1030, 1152, 1428, 1611
TseFI GTSAC 3 cut(s) 359, 412, 862
TseI GCWGC 9 cut(s) 34, 53, 56, 91, 174, 449, 665, 1231, 1789
Tsp45I GTSAC 3 cut(s) 359, 412, 862
TspDTI ATGAA 6 cut(s) 137, 199, 212, 951, 1021, 1814
TspGWI ACGGA 1 cut(s) 1002
TspRI CASTG 7 cut(s) 708, 735, 991, 1030, 1152, 1428, 1611
Van91I CCANNNNNTGG 1 cut(s) 1356
Vha464I CTTAAG 1 cut(s) 1108
VpaK11BI GGWCC 3 cut(s) 82, 1347, 1574
XapI RAATTY 3 cut(s) 756, 1118, 1487
XceI RCATGY 3 cut(s) 766, 848, 1316
XmiI GTMKAC 1 cut(s) 1446
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 1199, 1403
Zsp2I ATGCAT 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.