Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0011666

Physical Location & Seq

Genomic Coordinates Reverse (-)

20076738 .. 20080797

Gene Structure 4060 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g26961.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGGCAAAACTCTCCATACCCATACTGGGATTGCTACTGTTATGCTGCTTATCAGCTCTTGCAGAAGCCAAGTATTCCAAATACAAAGACCCAAAACAGCCCTTGAATGTTCGAATCAGGGACCTCATGAAGCGGATGACCCTGGCCGAGAAGATTGGCCAAATGGTCCAGATCGAGCGCGAGGTTGCTACCCCTGATGTCATGACCAAGTATTTGATAGGAAGTGTTCTCAGTGGTGGTGGGAGTGTGCCGGCTCCAAAAGCATCAGCTGCGACATGGGTCAATCTTGTCAATGGAATCCAAAAGGGATCCCTATCCACCCGTCTCGGGATTCCTATGATTTATGGGATTGATGCTGTCCATGGCCACAACAATGTCTATAATGCTACCATTTTCCCTCACAATGTTGGATTGGGAGCCACCAGAGATCCTGCGCTTATCAAGAGAATCGGAGAGGCGACTGCCCTTGAAGTCAGGGCAACAGGAATTCCTTATGCTTTTGCCCCATGTATCGCGGTTTGCAGGGATCCAAGATGGGGTAGGTGTTACGAAAGCTACAGTGAGGATCATAAGATTGTCCAAGCAATGACTGAGATCATCCCTGGTTTGCAAGGAGATATGCCTCCTAGTTTCCAAAAGGGAGCGCCTTATGTTGGTGGAAAGGGAAAGGTTGCAGCCTGTGCGAAGCACTATGTAGGAGATGGTGGCACAACCAGGGGCATCAATGAGAACAACACTGTGATTGATTGGAATGGTCTGCTTGGCATTCACATGCCCGCGTACCTCAACTCCATCCTTAAGGGTGTTGCAACAGTTATGGTCTCTTACTCCAGTTGGAATGGGAAGAAAATGCATGCTAATCAAGACCTTGTAACCGGATATCTCAAGAACAAGCTCCGTTTCAGGGGTTTCGTCATCTCAGATTGGGAGGGCATCGACAGGATTACATCTCCTCCCAAGGCTAACTATTCGTATTCAGTTCAAGCAGGAGTTAGTGCTGGAATTGACATGATCATGGTCCCCTACAACTTTACCGAGTTCATTGATGATCTGACATATCAAGTCAAGAATAATATCATCCCCATGAGCAGGATTGATGATGCTGTGAAGAGAATTTTGAGGGTTAAGTTTGTCATGGGCCTTTTTGAAAATCCCTTGGCAGATCTCAGCTTAGCCGATCAGCTAGGAAATAAGGAACATAGAGAATTGGCAAGAGAAGCGGTAAGAAAATCACTTGTTCTACTAAAGAATGGAAAATCTGCAACGAAGCCACTGCTTCCTCTATCCAAGAAAGCAGGGAAGATACTAATTGCAGGAAGTCATGCAGACAATTTGGGCTTTCAATGTGGAGGTTGGACGATCACATGGCAAGGCCTCGGTGGCAATGATCTTACAGTTGGTACCACAATCCTCAATGCGGTGAAAACTACAGTTGATCCTACTACTCAAGTTGTCTACAACGAGAGCCCTGATGCGAACTTTGTGAAGTCCAACAAATTCTCCCATGCTGTTGTTGTTGTGGGTGAACCTCCATACGCTGAAACCTTTGGAGACAGCATGAATTTGACCATATCTGAAGCAGGATTGAGCACAATCAACAATGTTTGTGGAGCTGTGAAATGTGTTGTGGTTATCATTTCGGGCCGCCCTGTTGTGGTCCAACCCTACCTTTCCAAAATTGATGCACTTGTTGCGGCTTGGCTTCCTGGAACTGAAGGTCAAGGTGTTGCTGACGTTTTATTTGGTGACTATGGATTCACCGGCAAGCTTGCACGTACATGGTTCAAATCAGTAGATCAGCTCCCAATGAATGTGGGTGATGCACATTATGACCCTCTATTTCCGTTTGGATTTGGTCTAACAACTAAGCCAACCAAGAATTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005623 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0044042 GO:0044238 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

629

Amino Acids

67.99

Weight (kDa)

9.09

Isoelectric Point (pI)

26.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	47 - 375	9.3e-67	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	412 - 621	3.9e-37	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20940 AT5G20950 AT5G20950 AT5G20950
fragaria_vesca	FvH4_3g26961
malus_domestica	MD03G1161800.v1.1 MD11G1178000.v1.1
prunus_persica	Prupe.6G150800_v2.0.a1 Prupe.6G150800_v2.0.a1
pyrus_communis	pycom11g15210
rosa_chinensis	RchiOBHm_Chr5g0049481
rosa_laevigata	RLG00000034621
rosa_multiflora	Rmu_sc0015493.1_g000005
rosa_roxburghii	Rroxscaffold_1G00032210
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325200 Rh5BG336300 Rh5DG348500
rosa_wichuraiana	Rw5G030700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1400
AccB1I	GGYRCC	1 cut(s)	1400
AccI	GTMKAC	1 cut(s)	1455
AccII	CGCG	3 cut(s)	180, 515, 779
AciI	CCGC	7 cut(s)	133, 515, 777, 1220, 1418, 1645, 1694
AclWI	GGATC	7 cut(s)	303, 316, 422, 521, 534, 573, 1430
AcoI	YGGCCR	3 cut(s)	144, 157, 364
AcsI	RAATTY	4 cut(s)	486, 1113, 1496, 1561
AcuI	CTGAAG	2 cut(s)	1596, 1734
AfaI	GTAC	3 cut(s)	782, 1402, 1777
AfiI	CCNNNNNNNGG	8 cut(s)	26, 327, 536, 653, 904, 1089, 1417, 1654
AflII	CTTAAG	1 cut(s)	797
AgsI	TTSAA	6 cut(s)	106, 470, 983, 1148, 1343, 1786
AjnI	CCWGG	4 cut(s)	141, 601, 713, 1705
AluBI	AGCT	9 cut(s)	56, 269, 555, 895, 1170, 1183, 1613, 1768, 1801
AluI	AGCT	9 cut(s)	56, 269, 555, 895, 1170, 1183, 1613, 1768, 1801
Alw21I	GWGCWC	1 cut(s)	1592
Alw26I	GTCTC	3 cut(s)	329, 826, 1545
AlwI	GGATC	7 cut(s)	303, 316, 422, 521, 534, 573, 1430
Ama87I	CYCGRG	1 cut(s)	326
AoxI	GGCC	6 cut(s)	144, 157, 364, 1138, 1372, 1642
ApeKI	GCWGC	3 cut(s)	45, 269, 674
ApoI	RAATTY	4 cut(s)	486, 1113, 1496, 1561
ArsI	GACNNNNNNTTYG	4 cut(s)	196, 228, 1724, 1756
Asp718I	GGTACC	1 cut(s)	1400
AspLEI	GCGC	3 cut(s)	180, 436, 646
AspS9I	GGNCC	6 cut(s)	121, 166, 1018, 1138, 1642, 1657
AsuHPI	GGTGA	5 cut(s)	1432, 1535, 1750, 1757, 1829
AsuII	TTCGAA	1 cut(s)	112
AvaI	CYCGRG	1 cut(s)	326
AvaII	GGWCC	4 cut(s)	121, 166, 1018, 1657
BalI	TGGCCA	2 cut(s)	159, 366
BamHI	GGATCC	2 cut(s)	308, 526
BanI	GGYRCC	1 cut(s)	1400
BanII	GRGCYC	1 cut(s)	1469
Bbv12I	GWGCWC	1 cut(s)	1592
BbvI	GCAGC	3 cut(s)	32, 256, 686
BccI	CCATC	3 cut(s)	528, 695, 800
BcgI	CGANNNNNNTGC	2 cut(s)	1255, 1289
BciT130I	CCWGG	4 cut(s)	143, 603, 715, 1707
BclI	TGATCA	1 cut(s)	1011
BcoDI	GTCTC	3 cut(s)	329, 826, 1545
BfaI	CTAG	3 cut(s)	627, 1184, 1885
BfmI	CTRYAG	2 cut(s)	556, 1428
BfoI	RGCGCY	1 cut(s)	647
BfrI	CTTAAG	1 cut(s)	797
BglI	GCCNNNNNGGC	1 cut(s)	1380
BglII	AGATCT	1 cut(s)	1162
BisI	GCNGC	5 cut(s)	46, 270, 675, 1645, 1695
BlpI	GCTNAGC	1 cut(s)	1171
BlsI	GCNGC	5 cut(s)	47, 271, 676, 1646, 1696
Bme1390I	CCNGG	4 cut(s)	143, 603, 715, 1707
Bme18I	GGWCC	4 cut(s)	121, 166, 1018, 1657
BmeT110I	CYCGRG	1 cut(s)	326
BmgT120I	GGNCC	6 cut(s)	121, 166, 1018, 1138, 1642, 1657
BmiI	GGNNCC	7 cut(s)	122, 255, 310, 418, 528, 1020, 1402
BmrFI	CCNGG	4 cut(s)	143, 603, 715, 1707
BmrI	ACTGGG	1 cut(s)	35
BmsI	GCATC	8 cut(s)	272, 343, 729, 942, 1090, 1462, 1672, 1810
BmuI	ACTGGG	1 cut(s)	35
BoxI	GACNNNNGTC	1 cut(s)	278
BpmI	CTGGAG	1 cut(s)	814
Bpu1102I	GCTNAGC	1 cut(s)	1171
Bpu14I	TTCGAA	1 cut(s)	112
BpuEI	CTTGAG	2 cut(s)	869, 1431
BsaAI	YACGTR	1 cut(s)	1775
BsaBI	GATNNNNATC	1 cut(s)	313
BsaI	GGTCTC	1 cut(s)	826
BsaJI	CCNNGG	7 cut(s)	141, 361, 601, 714, 957, 1155, 1375
BsaWI	WCCGGW	1 cut(s)	875
BsaXI	ACNNNNNCTCC	6 cut(s)	773, 803, 937, 967, 1484, 1514
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 327, 536, 653, 904, 1089, 1417, 1654
Bse118I	RCCGGY	2 cut(s)	250, 1760
Bse1I	ACTGG	2 cut(s)	30, 831
Bse3DI	GCAATG	2 cut(s)	591, 1390
Bse8I	GATNNNNATC	1 cut(s)	313
BseBI	CCWGG	4 cut(s)	143, 603, 715, 1707
BseDI	CCNNGG	7 cut(s)	141, 361, 601, 714, 957, 1155, 1375
BseGI	GGATG	4 cut(s)	141, 597, 792, 1077
BseJI	GATNNNNATC	1 cut(s)	313
BseLI	CCNNNNNNNGG	8 cut(s)	26, 327, 536, 653, 904, 1089, 1417, 1654
BseMI	GCAATG	2 cut(s)	591, 1390
BseMII	CTCAG	4 cut(s)	244, 582, 933, 1180
BseNI	ACTGG	2 cut(s)	30, 831
BseRI	GAGGAG	1 cut(s)	942
BseXI	GCAGC	3 cut(s)	32, 256, 686
Bsh1236I	CGCG	3 cut(s)	180, 515, 779
BshFI	GGCC	6 cut(s)	146, 159, 366, 1140, 1374, 1644
BshNI	GGYRCC	1 cut(s)	1400
BsiHKAI	GWGCWC	1 cut(s)	1592
BsiHKCI	CYCGRG	1 cut(s)	326
BsiSI	CCGG	3 cut(s)	251, 876, 1761
BslFI	GGGAC	2 cut(s)	134, 1004
BslI	CCNNNNNNNGG	8 cut(s)	26, 327, 536, 653, 904, 1089, 1417, 1654
BsmAI	GTCTC	3 cut(s)	329, 826, 1545
BsmBI	CGTCTC	1 cut(s)	329
BsmFI	GGGAC	2 cut(s)	134, 1004
BsmI	GAATGC	1 cut(s)	765
BsnI	GGCC	6 cut(s)	146, 159, 366, 1140, 1374, 1644
Bso31I	GGTCTC	1 cut(s)	826
BsoBI	CYCGRG	1 cut(s)	326
Bsp119I	TTCGAA	1 cut(s)	112
Bsp1286I	GDGCHC	2 cut(s)	1469, 1592
Bsp1720I	GCTNAGC	1 cut(s)	1171
Bsp19I	CCATGG	1 cut(s)	361
BspACI	CCGC	7 cut(s)	133, 515, 777, 1220, 1418, 1645, 1694
BspANI	GGCC	6 cut(s)	146, 159, 366, 1140, 1374, 1644
BspCNI	CTCAG	4 cut(s)	243, 583, 932, 1179
BspFNI	CGCG	3 cut(s)	180, 515, 779
BspHI	TCATGA	2 cut(s)	126, 201
BspLI	GGNNCC	7 cut(s)	122, 255, 310, 418, 528, 1020, 1402
BspPI	GGATC	7 cut(s)	303, 316, 422, 521, 534, 573, 1430
BspT104I	TTCGAA	1 cut(s)	112
BspT107I	GGYRCC	1 cut(s)	1400
BspTI	CTTAAG	1 cut(s)	797
BspTNI	GGTCTC	1 cut(s)	826
BsrDI	GCAATG	2 cut(s)	591, 1390
BsrFI	RCCGGY	2 cut(s)	250, 1760
BsrI	ACTGG	2 cut(s)	30, 831
BssAI	RCCGGY	2 cut(s)	250, 1760
BssECI	CCNNGG	7 cut(s)	141, 361, 601, 714, 957, 1155, 1375
BssT1I	CCWWGG	3 cut(s)	361, 957, 1155
Bst2UI	CCWGG	4 cut(s)	143, 603, 715, 1707
Bst4CI	ACNGT	6 cut(s)	39, 560, 739, 814, 1396, 1432
Bst6I	CTCTTC	1 cut(s)	1103
BstAFI	CTTAAG	1 cut(s)	797
BstAPI	GCANNNNNTGC	3 cut(s)	269, 680, 1691
BstBAI	YACGTR	1 cut(s)	1775
BstBI	TTCGAA	1 cut(s)	112
BstC8I	GCNNGC	5 cut(s)	252, 777, 855, 1766, 1770
BstDEI	CTNAG	6 cut(s)	230, 591, 919, 1166, 1171, 1866
BstDSI	CCRYGG	1 cut(s)	361
BstF5I	GGATG	4 cut(s)	141, 597, 792, 1077
BstFNI	CGCG	3 cut(s)	180, 515, 779
BstH2I	RGCGCY	1 cut(s)	647
BstHHI	GCGC	3 cut(s)	180, 436, 646
BstMAI	GTCTC	3 cut(s)	329, 826, 1545
BstMWI	GCNNNNNNNGC	6 cut(s)	260, 269, 680, 1217, 1380, 1691
BstNI	CCWGG	4 cut(s)	143, 603, 715, 1707
BstNSI	RCATGY	2 cut(s)	775, 857
BstPAI	GACNNNNGTC	1 cut(s)	278
BstSCI	CCNGG	4 cut(s)	141, 601, 713, 1705
BstSFI	CTRYAG	2 cut(s)	556, 1428
BstUI	CGCG	3 cut(s)	180, 515, 779
BstV1I	GCAGC	3 cut(s)	32, 256, 686
BstX2I	RGATCY	4 cut(s)	308, 427, 526, 1162
BstYI	RGATCY	4 cut(s)	308, 427, 526, 1162
BsuRI	GGCC	6 cut(s)	146, 159, 366, 1140, 1374, 1644
BtgI	CCRYGG	1 cut(s)	361
BtsCI	GGATG	4 cut(s)	141, 597, 792, 1077
BtsI	GCAGTG	1 cut(s)	1271
BtsIMutI	CAGTG	4 cut(s)	238, 565, 735, 1271
Cac8I	GCNNGC	5 cut(s)	252, 777, 855, 1766, 1770
CciI	TCATGA	2 cut(s)	126, 201
CfoI	GCGC	3 cut(s)	180, 436, 646
Cfr10I	RCCGGY	2 cut(s)	250, 1760
Cfr13I	GGNCC	6 cut(s)	121, 166, 1018, 1138, 1642, 1657
Csp6I	GTAC	3 cut(s)	781, 1401, 1776
CspCI	CAANNNNNGTGG	4 cut(s)	1588, 1623, 1794, 1829
CviQI	GTAC	3 cut(s)	781, 1401, 1776
DdeI	CTNAG	6 cut(s)	230, 591, 919, 1166, 1171, 1866
EaeI	YGGCCR	3 cut(s)	144, 157, 364
Eam1104I	CTCTTC	1 cut(s)	1103
EarI	CTCTTC	1 cut(s)	1103
Eco130I	CCWWGG	3 cut(s)	361, 957, 1155
Eco147I	AGGCCT	1 cut(s)	1374
Eco24I	GRGCYC	1 cut(s)	1469
Eco31I	GGTCTC	1 cut(s)	826
Eco32I	GATATC	1 cut(s)	881
Eco47I	GGWCC	4 cut(s)	121, 166, 1018, 1657
Eco57I	CTGAAG	2 cut(s)	1596, 1734
Eco88I	CYCGRG	1 cut(s)	326
EcoO109I	RGGNCCY	1 cut(s)	121
EcoRI	GAATTC	1 cut(s)	486
EcoRII	CCWGG	4 cut(s)	141, 601, 713, 1705
EcoRV	GATATC	1 cut(s)	881
EcoT14I	CCWWGG	3 cut(s)	361, 957, 1155
EcoT22I	ATGCAT	1 cut(s)	855
EcoT38I	GRGCYC	1 cut(s)	1469
ErhI	CCWWGG	3 cut(s)	361, 957, 1155
Esp3I	CGTCTC	1 cut(s)	329
FaqI	GGGAC	2 cut(s)	134, 1004
FauI	CCCGC	1 cut(s)	784
FbaI	TGATCA	1 cut(s)	1011
FblI	GTMKAC	1 cut(s)	1455
Fnu4HI	GCNGC	5 cut(s)	46, 270, 675, 1645, 1695
FokI	GGATG	4 cut(s)	148, 584, 779, 1064
FriOI	GRGCYC	1 cut(s)	1469
Fsp4HI	GCNGC	5 cut(s)	46, 270, 675, 1645, 1695
FspBI	CTAG	3 cut(s)	627, 1184, 1885
GlaI	GCGC	3 cut(s)	179, 435, 645
GluI	GCNGC	5 cut(s)	46, 270, 675, 1645, 1695
GsuI	CTGGAG	1 cut(s)	814
HaeII	RGCGCY	1 cut(s)	647
HaeIII	GGCC	6 cut(s)	146, 159, 366, 1140, 1374, 1644
HapII	CCGG	3 cut(s)	251, 876, 1761
HhaI	GCGC	3 cut(s)	180, 436, 646
Hin6I	GCGC	3 cut(s)	178, 434, 644
HinP1I	GCGC	3 cut(s)	178, 434, 644
HindIII	AAGCTT	1 cut(s)	1766
HinfI	GANTC	5 cut(s)	114, 297, 331, 447, 1755
HpaII	CCGG	3 cut(s)	251, 876, 1761
HphI	GGTGA	5 cut(s)	1432, 1535, 1750, 1757, 1829
Hpy166II	GTNNAC	2 cut(s)	1456, 1526
Hpy188I	TCNGA	4 cut(s)	452, 922, 1053, 1576
Hpy188III	TCNNGA	8 cut(s)	127, 169, 202, 328, 442, 863, 886, 1066
Hpy8I	GTNNAC	2 cut(s)	1456, 1526
HpyAV	CCTTC	1 cut(s)	1709
HpyCH4III	ACNGT	6 cut(s)	39, 560, 739, 814, 1396, 1432
HpyCH4IV	ACGT	2 cut(s)	1734, 1774
HpyF10VI	GCNNNNNNNGC	6 cut(s)	260, 269, 680, 1217, 1380, 1691
HpyF3I	CTNAG	6 cut(s)	230, 591, 919, 1166, 1171, 1866
HpySE526I	ACGT	2 cut(s)	1734, 1774
HspAI	GCGC	3 cut(s)	178, 434, 644
KpnI	GGTACC	1 cut(s)	1404
KroI	GCCGGC	1 cut(s)	250
KroNI	GCCGGC	1 cut(s)	252
Ksp22I	TGATCA	1 cut(s)	1011
LmnI	GCTCC	6 cut(s)	259, 416, 641, 900, 1610, 1806
Lsp1109I	GCAGC	3 cut(s)	32, 256, 686
LweI	GCATC	8 cut(s)	272, 343, 729, 942, 1090, 1462, 1672, 1810
MaeI	CTAG	3 cut(s)	627, 1184, 1885
MaeII	ACGT	2 cut(s)	1734, 1774
MaeIII	GTNAC	3 cut(s)	545, 871, 1745
MboII	GAAGA	4 cut(s)	163, 856, 1120, 1312
MflI	RGATCY	4 cut(s)	308, 427, 526, 1162
MhlI	GDGCHC	2 cut(s)	1469, 1592
MlsI	TGGCCA	2 cut(s)	159, 366
MluNI	TGGCCA	2 cut(s)	159, 366
MmeI	TCCRAC	5 cut(s)	388, 815, 1334, 1515, 1684
Mox20I	TGGCCA	2 cut(s)	159, 366
Mph1103I	ATGCAT	1 cut(s)	855
MroNI	GCCGGC	1 cut(s)	250
MscI	TGGCCA	2 cut(s)	159, 366
MseI	TTAA	2 cut(s)	798, 1125
MslI	CAYNNNNRTG	5 cut(s)	372, 770, 1013, 1082, 1777
Msp20I	TGGCCA	2 cut(s)	159, 366
MspA1I	CMGCKG	1 cut(s)	269
MspCI	CTTAAG	1 cut(s)	797
MspI	CCGG	3 cut(s)	251, 876, 1761
MspR9I	CCNGG	4 cut(s)	143, 603, 715, 1707
Mva1269I	GAATGC	1 cut(s)	765
MvaI	CCWGG	4 cut(s)	143, 603, 715, 1707
MvnI	CGCG	3 cut(s)	180, 515, 779
MwoI	GCNNNNNNNGC	6 cut(s)	260, 269, 680, 1217, 1380, 1691
NaeI	GCCGGC	1 cut(s)	252
NcoI	CCATGG	1 cut(s)	361
NgoMIV	GCCGGC	1 cut(s)	250
NlaIV	GGNNCC	7 cut(s)	122, 255, 310, 418, 528, 1020, 1402
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	1 cut(s)	1745
NsiI	ATGCAT	1 cut(s)	855
NspI	RCATGY	2 cut(s)	775, 857
NspV	TTCGAA	1 cut(s)	112
PaeI	GCATGC	1 cut(s)	857
PagI	TCATGA	2 cut(s)	126, 201
PceI	AGGCCT	1 cut(s)	1374
PctI	GAATGC	1 cut(s)	765
PdiI	GCCGGC	1 cut(s)	252
PfeI	GAWTC	5 cut(s)	114, 297, 331, 447, 1755
PfoI	TCCNGGA	1 cut(s)	1705
PkrI	GCNGC	5 cut(s)	47, 271, 676, 1646, 1696
Ppu21I	YACGTR	1 cut(s)	1775
PpuMI	RGGWCCY	1 cut(s)	121
PshAI	GACNNNNGTC	1 cut(s)	278
Psp5II	RGGWCCY	1 cut(s)	121
Psp6I	CCWGG	4 cut(s)	141, 601, 713, 1705
PspGI	CCWGG	4 cut(s)	141, 601, 713, 1705
PspN4I	GGNNCC	7 cut(s)	122, 255, 310, 418, 528, 1020, 1402
PspPI	GGNCC	6 cut(s)	121, 166, 1018, 1138, 1642, 1657
PspPPI	RGGWCCY	1 cut(s)	121
PsuI	RGATCY	4 cut(s)	308, 427, 526, 1162
PvuII	CAGCTG	1 cut(s)	269
RsaI	GTAC	3 cut(s)	782, 1402, 1777
RsaNI	GTAC	3 cut(s)	781, 1401, 1776
RseI	CAYNNNNRTG	5 cut(s)	372, 770, 1013, 1082, 1777
SaqAI	TTAA	2 cut(s)	798, 1125
SatI	GCNGC	5 cut(s)	46, 270, 675, 1645, 1695
Sau96I	GGNCC	6 cut(s)	121, 166, 1018, 1138, 1642, 1657
ScrFI	CCNGG	4 cut(s)	143, 603, 715, 1707
SduI	GDGCHC	2 cut(s)	1469, 1592
SfaNI	GCATC	8 cut(s)	272, 343, 729, 942, 1090, 1462, 1672, 1810
SfcI	CTRYAG	2 cut(s)	556, 1428
SfuI	TTCGAA	1 cut(s)	112
SinI	GGWCC	4 cut(s)	121, 166, 1018, 1657
SmiMI	CAYNNNNRTG	5 cut(s)	372, 770, 1013, 1082, 1777
SmlI	CTYRAG	3 cut(s)	797, 884, 1446
SmoI	CTYRAG	3 cut(s)	797, 884, 1446
SphI	GCATGC	1 cut(s)	857
SseBI	AGGCCT	1 cut(s)	1374
SsiI	CCGC	7 cut(s)	133, 515, 777, 1220, 1418, 1645, 1694
SspMI	CTAG	3 cut(s)	627, 1184, 1885
StuI	AGGCCT	1 cut(s)	1374
StyD4I	CCNGG	4 cut(s)	141, 601, 713, 1705
StyI	CCWWGG	3 cut(s)	361, 957, 1155
TaaI	ACNGT	6 cut(s)	39, 560, 739, 814, 1396, 1432
TaiI	ACGT	2 cut(s)	1737, 1777
TaqI	TCGA	3 cut(s)	112, 174, 936
TauI	GCSGC	2 cut(s)	1647, 1697
TfiI	GAWTC	5 cut(s)	114, 297, 331, 447, 1755
Tru1I	TTAA	2 cut(s)	798, 1125
Tru9I	TTAA	2 cut(s)	798, 1125
TscAI	CASTG	4 cut(s)	238, 565, 742, 1278
TseFI	GTSAC	1 cut(s)	1745
TseI	GCWGC	3 cut(s)	45, 269, 674
Tsp45I	GTSAC	1 cut(s)	1745
TspDTI	ATGAA	4 cut(s)	143, 1030, 1574, 1823
TspGWI	ACGGA	2 cut(s)	887, 1833
TspRI	CASTG	4 cut(s)	238, 565, 742, 1278
Vha464I	CTTAAG	1 cut(s)	797
VpaK11BI	GGWCC	4 cut(s)	121, 166, 1018, 1657
XapI	RAATTY	4 cut(s)	486, 1113, 1496, 1561
XceI	RCATGY	2 cut(s)	775, 857
XcmI	CCANNNNNNNNNTGG	1 cut(s)	22
XmiI	GTMKAC	1 cut(s)	1455
XspI	CTAG	3 cut(s)	627, 1184, 1885
Zsp2I	ATGCAT	1 cut(s)	855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.