AT5G22200

defense response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0023602
Physical Location & Seq
Forward (+)
7355570 .. 7357122
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22200.1

Sequence Viewer

Length: 633 bp
ATGACGGGGAGATACTGCGACCAACACAACGGCTATGAGGAGAGGAGGATGAGGATGATGATGAGACGCATAGCATGGGCATGTCTTGGCCTAATCGTGGCGGTTGCGTTCGTGGTGTTCTTGGTGTGGGCTATACTTCACCCGCACGGACCGCGTTTTGTCCTCCAAGACGTAACCATTAACGACTTCAACGTTTCTCAGCCAAATTTCCTCTCTTCAAATCTCCAGGTCACTGTATCTTCTCGCAATCCAAACGACAAGATCGGCATTTTTTATGATCGTCTCGACATCTACGTCACATACCGGAACCAAGAGGTAACGTTGGCGAGGCTCTTGCCGTCAACGTATCAAGGACATCTGGAAGTAACGGTGTGGTCACCATTTCTGATTGGCTCCGCCGTGCCGGTGGCTCCTTACCTATCTTCGGCATTGAACGAAGACTTATTCGCCGGGTTGGTGCTTCTCAACATCAAAATAGACGGTTGGGTTAGGTGGAAAGTGGGCTCGTGGGTCTCTGGCAGTTATCGCCTCCACGTTAACTGTCCTGCGTTCATTACCGTCACCGGAAAGTTAACCGGCACCGGTCCGGCGATCAAGTACCAGCTTGTTCAGCGATGTGCTGTTGACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

210

Amino Acids

23.74

Weight (kDa)

9.14

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 78 - 181 2.9e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023602)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44220 AT5G22200
prunus_persica Prupe.6G141400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 578
AccII CGCG 1 cut(s) 154
AciI CCGC 4 cut(s) 101, 143, 152, 396
AclI AACGTT 2 cut(s) 192, 320
AcsI RAATTY 1 cut(s) 205
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 2 cut(s) 97, 424
AgeI ACCGGT 1 cut(s) 581
AgsI TTSAA 3 cut(s) 190, 219, 433
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw26I GTCTC 3 cut(s) 58, 287, 517
AoxI GGCC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 205
AsiGI ACCGGT 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 149, 584
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 3 cut(s) 131, 369, 553
AvaII GGWCC 2 cut(s) 149, 584
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 506
BauI CACGAG 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 444
BceAI ACGGC 3 cut(s) 46, 322, 383
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 58, 287, 517
Bme1390I CCNGG 2 cut(s) 227, 451
Bme18I GGWCC 2 cut(s) 149, 584
BmgT120I GGNCC 2 cut(s) 149, 584
BmiI GGNNCC 4 cut(s) 308, 394, 411, 580
BmrFI CCNGG 2 cut(s) 227, 451
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 451
BsaI GGTCTC 1 cut(s) 517
BsaWI WCCGGW 3 cut(s) 303, 563, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 424
Bse118I RCCGGY 3 cut(s) 403, 575, 581
BseBI CCWGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 54, 60
BseLI CCNNNNNNNGG 2 cut(s) 97, 424
BseMII CTCAG 1 cut(s) 212
BseRI GAGGAG 2 cut(s) 53, 58
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 578
BshTI ACCGGT 1 cut(s) 581
BsiSI CCGG 7 cut(s) 304, 404, 450, 564, 576, 582, 587
BslI CCNNNNNNNGG 2 cut(s) 97, 424
BsmAI GTCTC 3 cut(s) 58, 287, 517
BsmBI CGTCTC 2 cut(s) 58, 287
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 3 cut(s) 261, 277, 591
BspACI CCGC 4 cut(s) 101, 143, 152, 396
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 4 cut(s) 308, 394, 411, 580
BspT107I GGYRCC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 517
BsrFI RCCGGY 3 cut(s) 403, 575, 581
BssAI RCCGGY 3 cut(s) 403, 575, 581
BssMI GATC 3 cut(s) 261, 277, 591
BssSI CACGAG 1 cut(s) 505
Bst2BI CACGAG 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 5 cut(s) 235, 370, 482, 542, 559
Bst6I CTCTTC 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 198
BstEII GGTNACC 1 cut(s) 375
BstF5I GGATG 2 cut(s) 54, 60
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 3 cut(s) 264, 280, 594
BstMAI GTCTC 3 cut(s) 58, 287, 517
BstMBI GATC 3 cut(s) 261, 277, 591
BstMWI GCNNNNNNNGC 3 cut(s) 151, 525, 610
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 84
BstPI GGTNACC 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 225, 449
BstUI CGCG 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 444
BsuRI GGCC 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 628
BtsCI GGATG 2 cut(s) 54, 60
BtsIMutI CAGTG 1 cut(s) 231
Cfr10I RCCGGY 3 cut(s) 403, 575, 581
Cfr13I GGNCC 2 cut(s) 149, 584
CpoI CGGWCCG 2 cut(s) 149, 584
CseI GACGC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 598
CspAI ACCGGT 1 cut(s) 581
CspI CGGWCCG 2 cut(s) 149, 584
CviAII CATG 2 cut(s) 75, 81
CviJI RGCY 9 cut(s) 33, 90, 131, 202, 331, 393, 410, 504, 604
CviKI_1 RGCY 9 cut(s) 33, 90, 131, 202, 331, 393, 410, 504, 604
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 1 cut(s) 198
DpnI GATC 3 cut(s) 263, 279, 593
DpnII GATC 3 cut(s) 261, 277, 591
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 506
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 149, 584
Eco91I GGTNACC 1 cut(s) 375
EcoO65I GGTNACC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 506
Esp3I CGTCTC 2 cut(s) 58, 287
FaeI CATG 2 cut(s) 78, 84
FaiI YATR 8 cut(s) 36, 71, 76, 82, 134, 276, 301, 631
FatI CATG 2 cut(s) 74, 80
FauI CCCGC 1 cut(s) 150
FokI GGATG 2 cut(s) 61, 67
FriOI GRGCYC 1 cut(s) 506
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 1 cut(s) 90
HapII CCGG 7 cut(s) 304, 404, 450, 564, 576, 582, 587
HgaI GACGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 78, 84
HincII GTYRAC 4 cut(s) 342, 538, 573, 625
HindII GTYRAC 4 cut(s) 342, 538, 573, 625
HpaI GTTAAC 2 cut(s) 538, 573
HpaII CCGG 7 cut(s) 304, 404, 450, 564, 576, 582, 587
HphI GGTGA 3 cut(s) 131, 369, 553
Hpy166II GTNNAC 4 cut(s) 342, 538, 573, 625
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 2 cut(s) 284, 359
Hpy8I GTNNAC 4 cut(s) 342, 538, 573, 625
HpyCH4III ACNGT 5 cut(s) 235, 370, 482, 542, 559
HpyCH4IV ACGT 7 cut(s) 171, 192, 294, 320, 344, 534, 627
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 525, 610
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 7 cut(s) 171, 192, 294, 320, 344, 534, 627
Hsp92II CATG 2 cut(s) 78, 84
KspAI GTTAAC 2 cut(s) 538, 573
Kzo9I GATC 3 cut(s) 261, 277, 591
LmnI GCTCC 2 cut(s) 398, 415
MaeII ACGT 7 cut(s) 171, 192, 294, 320, 344, 534, 627
MaeIII GTNAC 7 cut(s) 172, 229, 295, 316, 364, 375, 559
MalI GATC 3 cut(s) 263, 279, 593
MboI GATC 3 cut(s) 261, 277, 591
MboII GAAGA 4 cut(s) 207, 231, 414, 449
MhlI GDGCHC 1 cut(s) 506
MluCI AATT 1 cut(s) 205
MnlI CCTC 9 cut(s) 31, 36, 39, 45, 173, 221, 307, 321, 539
MseI TTAA 3 cut(s) 180, 537, 572
MslI CAYNNNNRTG 1 cut(s) 79
MspI CCGG 7 cut(s) 304, 404, 450, 564, 576, 582, 587
MspR9I CCNGG 2 cut(s) 227, 451
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 3 cut(s) 151, 525, 610
NciI CCSGG 1 cut(s) 451
NdeII GATC 3 cut(s) 261, 277, 591
NlaIII CATG 2 cut(s) 78, 84
NlaIV GGNNCC 4 cut(s) 308, 394, 411, 580
NmuCI GTSAC 4 cut(s) 229, 295, 375, 559
NspI RCATGY 1 cut(s) 84
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 291
PinAI ACCGGT 1 cut(s) 581
Psp1406I AACGTT 2 cut(s) 192, 320
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 375
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 4 cut(s) 308, 394, 411, 580
PspPI GGNCC 2 cut(s) 149, 584
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
RseI CAYNNNNRTG 1 cut(s) 79
Rsr2I CGGWCCG 2 cut(s) 149, 584
RsrII CGGWCCG 2 cut(s) 149, 584
SaqAI TTAA 3 cut(s) 180, 537, 572
Sau3AI GATC 3 cut(s) 261, 277, 591
Sau96I GGNCC 2 cut(s) 149, 584
ScrFI CCNGG 2 cut(s) 227, 451
SduI GDGCHC 1 cut(s) 506
SinI GGWCC 2 cut(s) 149, 584
SmiMI CAYNNNNRTG 1 cut(s) 79
Sse9I AATT 1 cut(s) 205
SsiI CCGC 4 cut(s) 101, 143, 152, 396
StyD4I CCNGG 2 cut(s) 225, 449
TaaI ACNGT 5 cut(s) 235, 370, 482, 542, 559
TaiI ACGT 7 cut(s) 174, 195, 297, 323, 347, 537, 630
TaqI TCGA 1 cut(s) 285
TasI AATT 1 cut(s) 205
Tru1I TTAA 3 cut(s) 180, 537, 572
Tru9I TTAA 3 cut(s) 180, 537, 572
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 4 cut(s) 229, 295, 375, 559
Tsp45I GTSAC 4 cut(s) 229, 295, 375, 559
TspDTI ATGAA 1 cut(s) 541
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 149, 584
XapI RAATTY 1 cut(s) 205
XceI RCATGY 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.