AT5G23210

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008965
Physical Location & Seq
Forward (+)
7810645 .. 7815089
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23210.1

Sequence Viewer

Length: 1500 bp
ATGGGTTCTCATAGTGTTGAGTTCTCTGTTCTTGTCCTATTTCTTGTGTCCTTTCTTCTTGGCTCAACATCAGCAGAAAAGCTGTGCAGTGACAACGACGGCGACAACGGTTGTTTCCGGTCTAGGGTTTTGGCAGCGCAACGAGCTGATCGAGTGAAAGAGCTTCCGGGACAGCCGCCAGTGAAATTCCGGCAATACGCCGGTTATGTGACGGTTAATGAAACACATGGAAGAGCATTGTTCTATTGGTTCTTTGAAGCCACTCAGAATCCAAGCAAAAAACCTGTCCTCCTTTGGCTCAATGGAGGACCGGGATGTTCATCAATCGGATTCGGAGCTGCAGAAGAACTGGGACCATTCTTCCCACAAAACTCGAGTCAGCCTAAACTCAAGCTAAATCCATACAGCTGGAACAAAGCGGCGAATTTGTTATTCTTGGAATCGCCGGTTGGAGTTGGATTCTCCTATACTAACACAAGCCGTGATATAAAACAACTCGGCGACACTGTCACAGCGAGAGACTCGTACAACTTCCTTGTCAACTGGTTCAAGAGATTTCCCCAGTACAAGTCACACGACTTCTACATTGCCGGAGAAAGCTACGCCGGCCATTACGTCCCACAACTTTCGGAGCTCATCTACAAAGAAAACAAGATTGCGTCCAAGAAAGATTTCATTAATCTCAAGGGTTTAATGATAGGAAACGCGTTATTGGATGACGAAACGGATCAAAAGGGGATGATAGAATATGCATGGGATCACGCGGTGATATCAGATGCATTGTACGAGAAAGTGAACAAAAACTGCGATTTCAAACAAAAACTTGTGACCAAGGAATGCAACGACGCTTTAGATGAGTATTTTGATGTATACAAGATCCTTGACATGTACAGTCTGTACGCTCCAAAGTGTGTCCCCACCTCCACGAACTCCTCTACTTCTCATTCAGTCGCCGGAAACCGCCCCCTACCCGCATTCCGAAGCATTCTACGACCCCGTCTCATCTCTCACAATGAAGGATGGAGGAGGATGGCCGCCGGGTACGATCCATGTGCATCAGAGTATACAGAAAAATATATGAACAGAAAAGATGTTCAGGAGGCACTTCACGCTAATGTCACCAACATCTCTTACCCTTGGACTCATTGCAGTGACACTGTCTCATTCTGGTCTGACGCTCCCGCTTCTATGCTTCCAACCTTAAGAACACTTGTTTCAGCGGGATTACGCGTATGGGTTTTCAGTGGGGATACAGACGGGCGAATCCCCGTGACCGCAACCAGATACTCACTAAAGAAGCTAGGTCTTAAGATAGTCCAAGACTGGACACCTTGGTACACCAAACTACAGGTCGGAGGGTGGACGGTGGAGTATGACGGTTTAATGTTTGTGACGGTAAGAGGAGCGGGACACCAGGTTCCGACATTTAAACCTAGAGAAGCTCTTCAGCTCATTCATCATTTTCTAGGCAATAAGAAACTTCCTACTTTTCCCTTTTGA

Protein Analysis

499

Amino Acids

56.33

Weight (kDa)

8.66

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 56 - 490 3e-148 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1406
AccI GTMKAC 2 cut(s) 870, 1064
AccII CGCG 3 cut(s) 707, 764, 1230
AclWI GGATC 4 cut(s) 735, 765, 871, 1040
AcoI YGGCCR 2 cut(s) 607, 1032
AcsI RAATTY 2 cut(s) 185, 424
AcuI CTGAAG 1 cut(s) 1430
AdeI CACNNNGTG 1 cut(s) 766
AfaI GTAC 7 cut(s) 527, 566, 785, 890, 899, 1043, 1337
AfiI CCNNNNNNNGG 1 cut(s) 124
AflII CTTAAG 2 cut(s) 1201, 1307
AflIII ACRYGT 3 cut(s) 705, 885, 1228
AgsI TTSAA 3 cut(s) 257, 550, 814
AjnI CCWGG 1 cut(s) 1413
AjuI GAANNNNNNNTTGG 4 cut(s) 432, 464, 656, 688
AloI GAACNNNNNNTCC 2 cut(s) 1401, 1433
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 3 cut(s) 513, 1004, 1165
AlwI GGATC 4 cut(s) 735, 765, 871, 1040
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 607, 1032
ApeKI GCWGC 2 cut(s) 134, 338
ApoI RAATTY 2 cut(s) 185, 424
ArsI GACNNNNNNTTYG 6 cut(s) 270, 302, 361, 393, 1335, 1367
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 2 cut(s) 671, 1443
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 2 cut(s) 308, 353
AsuC2I CCSGG 3 cut(s) 168, 312, 1039
AsuHPI GGTGA 2 cut(s) 778, 1111
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 2 cut(s) 308, 353
BanII GRGCYC 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 146, 325
BccI CCATC 2 cut(s) 1014, 1024
BceAI ACGGC 2 cut(s) 115, 465
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 1 cut(s) 1415
BciVI GTATCC 1 cut(s) 1243
BcnI CCSGG 3 cut(s) 168, 312, 1039
BcoDI GTCTC 3 cut(s) 513, 1004, 1165
BfaI CTAG 4 cut(s) 123, 1301, 1434, 1466
BfmI CTRYAG 2 cut(s) 339, 1346
BfrI CTTAAG 2 cut(s) 1201, 1307
BfuI GTATCC 1 cut(s) 1243
BisI GCNGC 5 cut(s) 135, 176, 339, 420, 1035
BlsI GCNGC 5 cut(s) 136, 177, 340, 421, 1036
Bme1390I CCNGG 4 cut(s) 168, 312, 1039, 1415
Bme18I GGWCC 2 cut(s) 308, 353
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 308, 353
BmiI GGNNCC 2 cut(s) 354, 1419
BmrFI CCNGG 4 cut(s) 168, 312, 1039, 1415
BmrI ACTGGG 2 cut(s) 359, 556
BmsI GCATC 2 cut(s) 766, 1064
BmuI ACTGGG 2 cut(s) 359, 556
BpuEI CTTGAG 2 cut(s) 374, 668
BpuMI CCSGG 3 cut(s) 168, 312, 1039
BsaBI GATNNNNATC 1 cut(s) 319
BsaJI CCNNGG 3 cut(s) 831, 1136, 1331
BsaWI WCCGGW 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 3 cut(s) 200, 445, 605
Bse1I ACTGG 5 cut(s) 179, 354, 548, 562, 1328
Bse3DI GCAATG 2 cut(s) 585, 1144
Bse8I GATNNNNATC 1 cut(s) 319
BseBI CCWGG 1 cut(s) 1415
BseDI CCNNGG 3 cut(s) 831, 1136, 1331
BseGI GGATG 5 cut(s) 320, 721, 744, 1025, 1035
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 2 cut(s) 585, 1144
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 5 cut(s) 179, 354, 548, 562, 1328
BseRI GAGGAG 3 cut(s) 922, 1039, 1416
BseXI GCAGC 2 cut(s) 146, 325
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 3 cut(s) 707, 764, 1230
BshFI GGCC 2 cut(s) 609, 1034
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 373
BslFI GGGAC 5 cut(s) 183, 366, 602, 899, 1422
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 3 cut(s) 513, 1004, 1165
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 5 cut(s) 183, 366, 602, 899, 1422
BsmI GAATGC 3 cut(s) 842, 974, 984
BsnI GGCC 2 cut(s) 609, 1034
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 636
Bsp1407I TGTACA 1 cut(s) 888
Bsp143I GATC 5 cut(s) 148, 727, 757, 876, 1045
BspANI GGCC 2 cut(s) 609, 1034
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 3 cut(s) 707, 764, 1230
BspLI GGNNCC 2 cut(s) 354, 1419
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 4 cut(s) 735, 765, 871, 1040
BspQI GCTCTTC 2 cut(s) 226, 1449
BspTI CTTAAG 2 cut(s) 1201, 1307
BsrBI CCGCTC 1 cut(s) 1406
BsrDI GCAATG 2 cut(s) 585, 1144
BsrFI RCCGGY 3 cut(s) 200, 445, 605
BsrGI TGTACA 1 cut(s) 888
BsrI ACTGG 5 cut(s) 179, 354, 548, 562, 1328
BssAI RCCGGY 3 cut(s) 200, 445, 605
BssECI CCNNGG 3 cut(s) 831, 1136, 1331
BssMI GATC 5 cut(s) 148, 727, 757, 876, 1045
BssNAI GTATAC 2 cut(s) 871, 1065
BssT1I CCWWGG 3 cut(s) 831, 1136, 1331
Bst1107I GTATAC 2 cut(s) 871, 1065
Bst2UI CCWGG 1 cut(s) 1415
Bst4CI ACNGT 8 cut(s) 110, 214, 508, 893, 1159, 1366, 1379, 1396
Bst6I CTCTTC 2 cut(s) 226, 1449
BstAFI CTTAAG 2 cut(s) 1201, 1307
BstAUI TGTACA 1 cut(s) 888
BstC8I GCNNGC 1 cut(s) 607
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 5 cut(s) 320, 721, 744, 1025, 1035
BstFNI CGCG 3 cut(s) 707, 764, 1230
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 5 cut(s) 151, 730, 760, 879, 1048
BstMAI GTCTC 3 cut(s) 513, 1004, 1165
BstMBI GATC 5 cut(s) 148, 727, 757, 876, 1045
BstMWI GCNNNNNNNGC 3 cut(s) 143, 606, 1109
BstNI CCWGG 1 cut(s) 1415
BstNSI RCATGY 1 cut(s) 889
BstSCI CCNGG 4 cut(s) 166, 310, 1037, 1413
BstSFI CTRYAG 2 cut(s) 339, 1346
BstUI CGCG 3 cut(s) 707, 764, 1230
BstV1I GCAGC 2 cut(s) 146, 325
BstX2I RGATCY 1 cut(s) 876
BstXI CCANNNNNNTGG 1 cut(s) 408
BstYI RGATCY 1 cut(s) 876
BstZ17I GTATAC 2 cut(s) 871, 1065
BsuI GTATCC 1 cut(s) 1243
BsuRI GGCC 2 cut(s) 609, 1034
BtsCI GGATG 5 cut(s) 320, 721, 744, 1025, 1035
BtsI GCAGTG 2 cut(s) 94, 1156
BtsIMutI CAGTG 6 cut(s) 94, 186, 504, 1155, 1156, 1249
Cac8I GCNNGC 1 cut(s) 607
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 3 cut(s) 200, 445, 605
Cfr13I GGNCC 2 cut(s) 308, 353
CseI GACGC 3 cut(s) 648, 854, 1184
CsiI ACCWGGT 1 cut(s) 1413
Csp6I GTAC 7 cut(s) 526, 565, 784, 889, 898, 1042, 1336
CviAII CATG 4 cut(s) 227, 753, 886, 1050
CviQI GTAC 7 cut(s) 526, 565, 784, 889, 898, 1042, 1336
DdeI CTNAG 1 cut(s) 264
DpnI GATC 5 cut(s) 150, 729, 759, 878, 1047
DpnII GATC 5 cut(s) 148, 727, 757, 876, 1045
DraI TTTAAA 1 cut(s) 1429
DraIII CACNNNGTG 1 cut(s) 766
EaeI YGGCCR 2 cut(s) 607, 1032
Eam1104I CTCTTC 2 cut(s) 226, 1449
EarI CTCTTC 2 cut(s) 226, 1449
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 3 cut(s) 831, 1136, 1331
Eco24I GRGCYC 1 cut(s) 636
Eco32I GATATC 1 cut(s) 771
Eco47I GGWCC 2 cut(s) 308, 353
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 1430
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 1413
EcoRV GATATC 1 cut(s) 771
EcoT14I CCWWGG 3 cut(s) 831, 1136, 1331
EcoT22I ATGCAT 2 cut(s) 754, 781
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 3 cut(s) 831, 1136, 1331
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 4 cut(s) 230, 756, 889, 1053
FaqI GGGAC 5 cut(s) 183, 366, 602, 899, 1422
FatI CATG 4 cut(s) 226, 752, 885, 1049
FauI CCCGC 4 cut(s) 979, 1189, 1213, 1399
FblI GTMKAC 2 cut(s) 870, 1064
Fnu4HI GCNGC 5 cut(s) 135, 176, 339, 420, 1035
FokI GGATG 5 cut(s) 327, 728, 751, 1032, 1042
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 5 cut(s) 135, 176, 339, 420, 1035
FspBI CTAG 4 cut(s) 123, 1301, 1434, 1466
GlaI GCGC 1 cut(s) 138
GluI GCNGC 5 cut(s) 135, 176, 339, 420, 1035
HaeIII GGCC 2 cut(s) 609, 1034
HgaI GACGC 3 cut(s) 648, 854, 1184
HhaI GCGC 1 cut(s) 139
Hin1II CATG 4 cut(s) 230, 756, 889, 1053
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 8 cut(s) 268, 330, 376, 440, 459, 521, 1141, 1263
HphI GGTGA 2 cut(s) 778, 1111
Hpy166II GTNNAC 6 cut(s) 541, 796, 871, 1065, 1338, 1362
Hpy188III TCNNGA 2 cut(s) 550, 1097
Hpy8I GTNNAC 6 cut(s) 541, 796, 871, 1065, 1338, 1362
Hpy99I CGWCG 2 cut(s) 101, 848
HpyAV CCTTC 1 cut(s) 1010
HpyCH4III ACNGT 8 cut(s) 110, 214, 508, 893, 1159, 1366, 1379, 1396
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 87, 341, 752, 779, 840, 1055, 1149
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 606, 1109
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 4 cut(s) 230, 756, 889, 1053
HspAI GCGC 1 cut(s) 137
KroI GCCGGC 1 cut(s) 605
KroNI GCCGGC 1 cut(s) 607
Kzo9I GATC 5 cut(s) 148, 727, 757, 876, 1045
LguI GCTCTTC 2 cut(s) 226, 1449
LmnI GCTCC 5 cut(s) 335, 631, 907, 1183, 1403
Lsp1109I GCAGC 2 cut(s) 146, 325
LweI GCATC 2 cut(s) 766, 1064
MabI ACCWGGT 1 cut(s) 1413
MaeI CTAG 4 cut(s) 123, 1301, 1434, 1466
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 9 cut(s) 89, 208, 508, 570, 826, 1117, 1151, 1270, 1390
MalI GATC 5 cut(s) 150, 729, 759, 878, 1047
MbiI CCGCTC 1 cut(s) 1406
MboI GATC 5 cut(s) 148, 727, 757, 876, 1045
MboII GAAGA 5 cut(s) 47, 243, 352, 356, 1436
MflI RGATCY 1 cut(s) 876
MhlI GDGCHC 1 cut(s) 636
MluCI AATT 2 cut(s) 185, 424
MluI ACGCGT 2 cut(s) 705, 1228
MlyI GAGTC 3 cut(s) 385, 515, 1135
MmeI TCCRAC 5 cut(s) 430, 436, 1220, 1333, 1445
MnlI CCTC 9 cut(s) 299, 299, 931, 943, 1017, 1020, 1093, 1349, 1394
Mph1103I ATGCAT 2 cut(s) 754, 781
MroNI GCCGGC 1 cut(s) 605
MroXI GAANNNNTTC 2 cut(s) 671, 1443
MseI TTAA 7 cut(s) 216, 678, 692, 1202, 1308, 1382, 1428
MslI CAYNNNNRTG 2 cut(s) 1113, 1149
MspA1I CMGCKG 2 cut(s) 408, 1220
MspCI CTTAAG 2 cut(s) 1201, 1307
MspR9I CCNGG 4 cut(s) 168, 312, 1039, 1415
Mva1269I GAATGC 3 cut(s) 842, 974, 984
MvaI CCWGG 1 cut(s) 1415
MvnI CGCG 3 cut(s) 707, 764, 1230
MwoI GCNNNNNNNGC 3 cut(s) 143, 606, 1109
NaeI GCCGGC 1 cut(s) 607
NciI CCSGG 3 cut(s) 168, 312, 1039
NdeII GATC 5 cut(s) 148, 727, 757, 876, 1045
NgoMIV GCCGGC 1 cut(s) 605
NlaIII CATG 4 cut(s) 230, 756, 889, 1053
NlaIV GGNNCC 2 cut(s) 354, 1419
NmeAIII GCCGAG 1 cut(s) 477
NmuCI GTSAC 9 cut(s) 89, 208, 508, 570, 826, 1117, 1151, 1270, 1390
NsiI ATGCAT 2 cut(s) 754, 781
NspI RCATGY 1 cut(s) 889
PaeR7I CTCGAG 1 cut(s) 373
PciI ACATGT 1 cut(s) 885
PciSI GCTCTTC 2 cut(s) 226, 1449
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PctI GAATGC 3 cut(s) 842, 974, 984
PdiI GCCGGC 1 cut(s) 607
PdmI GAANNNNTTC 2 cut(s) 671, 1443
PfeI GAWTC 5 cut(s) 268, 330, 440, 459, 1263
PflFI GACNNNGTC 3 cut(s) 506, 996, 1157
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 5 cut(s) 136, 177, 340, 421, 1036
PleI GAGTC 3 cut(s) 384, 515, 1135
PpsI GAGTC 3 cut(s) 384, 515, 1135
PscI ACATGT 1 cut(s) 885
PshBI ATTAAT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 1413
PspGI CCWGG 1 cut(s) 1413
PspN4I GGNNCC 2 cut(s) 354, 1419
PspPI GGNCC 2 cut(s) 308, 353
PspXI VCTCGAGB 1 cut(s) 373
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 876
PsyI GACNNNGTC 3 cut(s) 506, 996, 1157
PvuII CAGCTG 1 cut(s) 408
RsaI GTAC 7 cut(s) 527, 566, 785, 890, 899, 1043, 1337
RsaNI GTAC 7 cut(s) 526, 565, 784, 889, 898, 1042, 1336
RseI CAYNNNNRTG 2 cut(s) 1113, 1149
SacI GAGCTC 1 cut(s) 636
SapI GCTCTTC 2 cut(s) 226, 1449
SaqAI TTAA 7 cut(s) 216, 678, 692, 1202, 1308, 1382, 1428
SatI GCNGC 5 cut(s) 135, 176, 339, 420, 1035
Sau3AI GATC 5 cut(s) 148, 727, 757, 876, 1045
Sau96I GGNCC 2 cut(s) 308, 353
SchI GAGTC 3 cut(s) 385, 515, 1135
ScrFI CCNGG 4 cut(s) 168, 312, 1039, 1415
SduI GDGCHC 1 cut(s) 636
SexAI ACCWGGT 1 cut(s) 1413
SfaNI GCATC 2 cut(s) 766, 1064
SfcI CTRYAG 2 cut(s) 339, 1346
Sfr274I CTCGAG 1 cut(s) 373
SinI GGWCC 2 cut(s) 308, 353
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 1113, 1149
SmlI CTYRAG 5 cut(s) 373, 389, 683, 1201, 1307
SmoI CTYRAG 5 cut(s) 373, 389, 683, 1201, 1307
Sse9I AATT 2 cut(s) 185, 424
SspMI CTAG 4 cut(s) 123, 1301, 1434, 1466
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 4 cut(s) 166, 310, 1037, 1413
StyI CCWWGG 3 cut(s) 831, 1136, 1331
TaaI ACNGT 8 cut(s) 110, 214, 508, 893, 1159, 1366, 1379, 1396
TaiI ACGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 151, 374
TasI AATT 2 cut(s) 185, 424
TatI WGTACW 2 cut(s) 564, 888
TauI GCSGC 3 cut(s) 178, 422, 1037
TfiI GAWTC 5 cut(s) 268, 330, 440, 459, 1263
Tru1I TTAA 7 cut(s) 216, 678, 692, 1202, 1308, 1382, 1428
Tru9I TTAA 7 cut(s) 216, 678, 692, 1202, 1308, 1382, 1428
TscAI CASTG 6 cut(s) 94, 186, 511, 1156, 1162, 1249
TseFI GTSAC 9 cut(s) 89, 208, 508, 570, 826, 1117, 1151, 1270, 1390
TseI GCWGC 2 cut(s) 134, 338
Tsp45I GTSAC 9 cut(s) 89, 208, 508, 570, 826, 1117, 1151, 1270, 1390
TspDTI ATGAA 6 cut(s) 234, 309, 664, 1029, 1094, 1445
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 6 cut(s) 94, 186, 511, 1156, 1162, 1249
Tth111I GACNNNGTC 3 cut(s) 506, 996, 1157
Vha464I CTTAAG 2 cut(s) 1201, 1307
VpaK11BI GGWCC 2 cut(s) 308, 353
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 2 cut(s) 185, 424
XceI RCATGY 1 cut(s) 889
XhoI CTCGAG 1 cut(s) 373
XmiI GTMKAC 2 cut(s) 870, 1064
XmnI GAANNNNTTC 2 cut(s) 671, 1443
XspI CTAG 4 cut(s) 123, 1301, 1434, 1466
Zsp2I ATGCAT 2 cut(s) 754, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.